Simultaneous Determination of Four Compounds, Campesterol, Emodin8-O-β-D-Glucopyranoside, Quercetin, and Isoquercitrin in <i>Reynoutria sachalinensis</i> by High-performance Liquid Chromatography-Diode Array Detector

Eom, Min Rye,Weon, Jin Bae,Jung, Youn Sik,Ryu, Ga Hee,Yang, Woo Seung,Ma, Choong Je Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.50

<P><B>Background:</B></P><P><I>Reynoutria sachalinensis</I> is a well-known and used herbal medicine to treatment of arthralgia, jaundice, amenorrhea, coughs, carbuncles, and sores.</P><P><B>Objective:</B></P><P>We have developed high-performance liquid chromatography analysis method for simultaneous determination of isolated four compounds, campesterol, emodin8-O-β-D-glucopyranoside, quercetin, and isoquercitrin from <I>R. sachalinensis</I> is.</P><P><B>Materials and Methods:</B></P><P>The four compounds were separated on Shiseido C<SUB>18</SUB> column (S-5 μm, 4.6 mm I.D. ×250 mm) at a column temperature of 25°C. The mobile phase composed of water and methanol with gradient elution system, and flow rate is 1.0 ml/min. The detection wavelength was set at 205 nm.</P><P><B>Results:</B></P><P>Validation of this analytical method was evaluated by linearity, precision, and accuracy test. This established method had good linearity (<I>R</I><SUP>2</SUP> > 0.997). The relative standard deviation values of intra- and inter-day testing were indicated that <2%, and accuracy is 91.66%–103.31% at intraday and 91.69%–103.31% at intraday. The results of recovery test were 92.60%–108.99%.</P><P><B>Conclusion:</B></P><P>In these results, developed method was accurate and reliable to the quality evaluation of campesterol, emodin 8-O-β-D-glucopyranoside, quercetin, and isoquercitrin isolated from <I>R. sachalinensis</I>.</P><P><B>SUMMARY</B></P><P><P>We have developed high-performance liquid analysis method for simultaneous determination of 4 compounds of <I>Reynoutria sachalinensis</I>.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> HPLC: High-performance liquid chromatography, DAD: Diode array detector, LOD: Limit of detection, LOQ: Limit of quantitation, ICH: International Conference on Harmonisation.</P>

Endoscopically Applied Biodegradable Stent in a Rabbit Model of Pediatric Tracheomalacia

최지숙,석준걸,Min Rye Eom,정은지,박수아,주상민,전여진,손길원,권성근 대한이비인후과학회 2021 Clinical and Experimental Otorhinolaryngology Vol.14 No.3

Objectives. A polydioxanone (PDO) stent was developed to treat tracheomalacia in pediatric patients. However, its safety and efficacy need to be verified in animal studies before clinical trials in patients can be conducted. This study evaluated the safety and efficacy of a PDO stent in normal and tracheomalacia-model rabbits. Methods. In total, 29 New Zealand white rabbits were used: 13 for evaluating the biocompatibility of the PDO stent in normal rabbits and 16 for the creation of a tracheomalacia model. The tracheomalacia model was successfully established in 12 rabbits, and PDO stents were placed in eight of those rabbits. Results. The PDO stent was successfully positioned in the trachea of the normal rabbits using an endoscopic approach, and its degradation was observed 10 weeks later. The stent fragments did not induce distal airway obstruction or damage, and the mucosal changes that occurred after stent placement were reversed after degradation. The same procedure was performed on the tracheomalacia-model rabbits. The survival duration of the tracheomalacia rabbits with and without stents was 49.0±6.8 and 1.0±0.8 days, respectively. Thus, the PDO stent yielded a significant survival gain (P=0.001). In the tracheomalacia rabbits, stent degradation and granulation tissue were observed 7 weeks after placement, leading to airway collapse and death. Conclusion. We successfully developed a PDO stent and an endoscopic guide placement system. The degradation time of the stent was around 10 weeks in normal rabbits, and its degradation was accelerated in the tracheomalacia model. The mucosal changes associated with PDO stent placement were reversible. Placement of the PDO stent prolonged survival in tracheomalacia-model rabbits.

Multiple stimulation with spheroids comprising salivary gland and adipose-derived stem cells enhances regeneration of radiation-damaged salivary glands

Jooyoung Kim,Min Rye Eom,Eun Ji Jeong,최지숙,권성근 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

Tissue engineering approaches using a single stem cell type have been unsuccessful in achievingregeneration of radiation-damaged salivary glands (SGs). Therefore, we combined adult SG stem cells(SGSCs) and adipose-derived stem cells (ASCs) in optimized spheroids to enhance the regeneration ofdamaged SGs in mice. SGSC/ASC spheroids survived longer and secreted more vascular endothelialgrowth factor (VEGF) than did SGSC spheroids. To enhance cell viability and differentiation, SGSC/ASCspheroids were stimulated with the Wnt/b-catenin signaling activator CHIR99021 and retinoic acid inhibitorBMS431 to maintain stemness. FGF7/10 induced the differentiation of multiple stimulated SGSC/ASC spheroids. SGSC/ASC spheroids exhibited improved viability, calcium channel function, differentiation,and VEGF secretion after multiple, sequential stimulation. The 21-day survival rate of multiply stimulatedSGSC/ASC spheroids was two-fold that of non-treated SGSC/ASC spheroids. Optimized SGSC/ASCspheroids enhanced the in vivo regeneration and functional recovery of SG tissue in mice by promotingneovascularization. Genes related to VEGF, such as Vegfa and Vegfb, were highly expressed 26 times forin vitro and 1.5 times for in vivo experiment in the multiply stimulated SGSC/ASC spheroids. Therefore,SGSC/ASC spheroids that have undergone multiple stimulation may be useful for healing radiationdamagedSGs.

Steamed and Fermented Ethanolic Extract from <i> Codonopsis lanceolata</i> Attenuates Amyloid- <i>β</i> -Induced Memory Impairment in Mice

Weon, Jin Bae,Eom, Min Rye,Jung, Youn Sik,Hong, Eun-Hye,Ko, Hyun-Jeong,Lee, Hyeon Yong,Park, Dong-Sik,Ma, Choong Je Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P><I>Codonopsis lanceolata</I> (<I>C. lanceolata</I>) is a traditional medicinal plant used for the treatment of certain inflammatory diseases such as asthma, tonsillitis, and pharyngitis. We evaluated whether steamed and fermented<I> C. lanceolata</I> (SFC) extract improves amyloid-<I>β</I>- (A<I>β</I>-) induced learning and memory impairment in mice. The Morris water maze and passive avoidance tests were used to evaluate the effect of SFC extract. Moreover, we investigated acetylcholinesterase (AChE) activity and brain-derived neurotrophic factor (BDNF), cyclic AMP response element-binding protein (CREB), and extracellular signal-regulated kinase (ERK) signaling in the hippocampus of mice to determine a possible mechanism for the cognitive-enhancing effect. Saponin compounds in SFC were identified by Ultra Performance Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS). SFC extract ameliorated amyloid-<I>β</I>-induced memory impairment in the Morris water maze and passive avoidance tests. SFC extract inhibited AChE activity and also significantly increased the level of CREB phosphorylation, BDNF expression, and ERK activation in hippocampal tissue of amyloid-<I>β</I>-treated mice. Lancemasides A, B, C, D, E, and G and foetidissimoside A compounds present in SFC were determined by UPLC-Q-TOF-MS. These results indicate that SFC extract improves A<I>β</I>-induced memory deficits and that AChE inhibition and CREB/BDNF/ERK expression is important for the effect of the SFC extract. In addition, lancemaside A specifically may be responsible for efficacious effect of SFC.</P>

Hyaluronic Acid Coating on Hydrophobic Tracheal Scaffold Enhances Mesenchymal Stem Cell Adhesion and Tracheal Regeneration

Choi Ji Suk,Lee Min Sang,Kim Jooyoung,Eom Min Rye,Jeong Eun Ji,Lee Minhyung,Park Su A,Jeong Ji Hoon,Kwon Seong Keun 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.2

BACKGROUND: Long segmental tracheal repair is challenging in regenerative medicine due to low adhesion of stem cells to tracheal scaffolds. Optimal transplantation of stem cells for tracheal defects has not been established. We evaluated the role of hyaluronic acid (HA) coating of tracheal scaffolds in mesenchymal stem cell (MSC) adhesion and tracheal regeneration in a rabbit model. METHODS: A three-dimensionally printed tubular tracheal prosthesis was incubated with dopa-HA-fluorescein isothiocyanate in phosphate-buffered saline for 2 days. MSCs were incubated with an HA-coated scaffold, and their adhesion was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HA coated scaffolds with or without MSC seeding were transplanted at the circumferential tracheal defect in rabbits, and survival, rigid bronchoscopy, radiologic findings, and histologic findings were compared between the two groups. RESULTS: HA-coated scaffolds showed better MSC adhesion than non-coated scaffolds. The HA-coated scaffolds with MSC group showed a wider airway and greater mucosal regeneration compared to the HA-coated scaffolds without MSC group. CONCLUSION: HA coating of scaffolds can promote MSC adhesion and tracheal regeneration. BACKGROUND: Long segmental tracheal repair is challenging in regenerative medicine due to low adhesion of stem cells to tracheal scaffolds. Optimal transplantation of stem cells for tracheal defects has not been established. We evaluated the role of hyaluronic acid (HA) coating of tracheal scaffolds in mesenchymal stem cell (MSC) adhesion and tracheal regeneration in a rabbit model. METHODS: A three-dimensionally printed tubular tracheal prosthesis was incubated with dopa-HA-fluorescein isothiocyanate in phosphate-buffered saline for 2 days. MSCs were incubated with an HA-coated scaffold, and their adhesion was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HA coated scaffolds with or without MSC seeding were transplanted at the circumferential tracheal defect in rabbits, and survival, rigid bronchoscopy, radiologic findings, and histologic findings were compared between the two groups. RESULTS: HA-coated scaffolds showed better MSC adhesion than non-coated scaffolds. The HA-coated scaffolds with MSC group showed a wider airway and greater mucosal regeneration compared to the HA-coated scaffolds without MSC group. CONCLUSION: HA coating of scaffolds can promote MSC adhesion and tracheal regeneration.

Injection laryngoplasty of human adipose-derived stem cell spheroids with hyaluronic acid-based hydrogel improves the morphological and functional characteristics of geriatric larynx

이도영,Young Hwan Choi,최지숙,Min Rye Eom,권성근 한국생체재료학회 2022 생체재료학회지 Vol.26 No.2

Aim: As the geriatric population increased, the need of treatment for laryngeal atrophy and dysfunction increased. This study was performed to evaluate the effects of injection of human adipose-derived stem cell (hASC) spheroidloaded catechol-conjugated hyaluronic acid (HA-CA) hydrogel on therapeutic rejuvenation of the geriatric larynx. Methods: Stem cell spheroids with hyaluronic acid-based hydrogel were injected into the laryngeal muscles of 18-month-old Sprague–Dawley rats. The effects of hASC spheroids were examined in the following four groups: SHAM, injected with PBS; GEL, injected with HA-CA hydrogel; MONO, injected with single hASCs in HA-CA hydrogel; and SP, injected with hASCs spheroids in HA-CA hydrogel. The rejuvenation efficacy in geriatric laryngeal muscle tissues at 12 weeks postinjection was evaluated and compared by histology, immunofluorescence staining, and functionality analysis. Results: Total myofiber cross-sectional area and myofiber number/density, evaluated by detection of myosin heavy chain with antibodies against laminin and fast myosin heavy chain, were significantly higher in the SP group than in the other groups. The lamina propria of the larynx was evaluated by alcian blue staining, which showed that the HA was increased significantly in the SP group compared to the other groups. In functional analysis, the glottal gap area was significantly reduced in the SP group compared to the other groups. The phase difference in the vocal fold during vibration was also smaller in the SP group than in the other groups, but the difference did not reach statistical significance. Conclusion: Injection of hASC spheroids with hyaluronic acid-based hydrogel improves the morphological and functional characteristics of geriatric larynx.

Dexamethasone loaded bilayered 3D tubular scaffold reduces restenosis at the anastomotic site of tracheal replacement: <i>in vitro</i> and <i>in vivo</i> assessments

Lee, Sang Jin,Choi, Ji Suk,Eom, Min Rye,Jo, Ha Hyeon,Kwon, Il Keun,Kwon, Seong Keun,Park, Su A The Royal Society of Chemistry 2020 Nanoscale Vol.12 No.8

<P>Despite recent developments in the tracheal tissue engineering field, the creation of a patient specific substitute possessing both appropriate mechanical and biointerfacial properties remains challenging. Most tracheal replacement therapies fail due to restenosis at the anastomosis site. In this study, we designed a robust, biodegradable, 3D tubular scaffold by combining electrospinning (ELSP) and 3D (three-dimensional) printing techniques for use in transplantation therapy. After that, we loaded dexamethasone (DEX) onto the 3D tubular scaffold using mild surface modification reactions by using polydopamine (PDA), polyethyleneimine (PEI), and carboxymethyl-β-cyclodextrin (βCD). As a result, the fabricated 3D tubular scaffold had robust mechanical properties and the chemical modifications were confirmed to have proceeded successfully by physico-chemical analysis. The surface treatments allowed for a larger amount of DEX to be loaded onto the βCD modified scaffold as compared to the bare group. <I>In vitro</I> and <I>in vivo</I> studies demonstrated that the DEX loaded 3D tubular scaffold exhibited significantly enhanced anti-inflammation activity, enhanced tracheal mucosal regeneration, and formation of a patent airway. From our results, we believe that our system may represent an innovative paradigm in tracheal tissue engineering by providing proper mechanical properties and successful formation of tracheal tissue as a means of remodeling and healing tracheal defects for use in transplantation therapy.</P>

The Ameliorating Effect of Steamed and Fermented <i>Codonopsis lanceolata</i> on Scopolamine-Induced Memory Impairment in Mice

Weon, Jin Bae,Yun, Bo-Ra,Lee, Jiwoo,Eom, Min Rye,Kim, Ji Seon,Lee, Hyeon Yong,Park, Dong-Sik,Chung, Hee-Chul,Chung, Jae Youn,Ma, Choong Je Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P><I>Codonopsis lanceolata</I> (Campanulaceae) have been traditionally used to treat lung inflammatory diseases, such as asthma, tonsillitis, and pharyngitis. The present study was performed to evaluate the cognitive-enhancing effects of steamed and fermented <I>C. lanceolata</I> in scopolamine-induced memory impairments in mice. Cognitive abilities were determined by the Morris water maze and passive avoidance tests. Mice orally received fermented <I>C. lanceolata</I> extract at doses of 100, 300, or 500 mg/kg body weight. Fermented <I>C. lanceolata</I> extract (500 mg/kg body weight, p.o.) significantly shortened the escape latency times that were increased by scopolamine on the 4th day of trial sessions in the Morris water maze task. In addition, it exerted longer step-through latency times than those of the scopolamine-treated group in the passive avoidance test. Furthermore, the neuroprotective effects of fermented <I>C. lanceolata</I> extract on glutamate-induced neurocytotoxicity were investigated in HT22 cells. Fermented <I>C. lanceolata</I> extract showed a relative protection ratio of 59.62% at 500 <I><I>μ</I></I>g/mL. In conclusion, fermented <I>C. lanceolata</I> extract ameliorated scopolamine-induced memory impairments, exerted neuroprotective effects, and improved activity compared to that found with original <I>C. lanceolata</I>. Further study will be required to investigate the mechanisms underlying this cognitive-enhancing activity.</P>

Adipose-derived mesenchymal stem cell spheroid sheet accelerates regeneration of ulcerated oral mucosa by enhancing inherent therapeutic properties

Jennifer Sang-jee Lee,Se-Jeong Kim,최지숙,Min Rye Eom,Heungsoo Shin,Seong Keun Kwon 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.91 No.-

The goal of this study was to investigate the effect of transplantation of an adipose-derived stem cell(ASC) spheroid sheet on the regeneration of ulcerated oral mucosa. The ASC spheroid sheet wasconstructed by seeding three-dimensional (3D) spheroidal ASCs on a temperature-responsive hydrogel,where cell adhesion and spreading were improved by incorporatingfibronectin. The 3D culture of ASCsenhanced their inherent paracrine properties for up to 1 week after transferring to two-dimensional (2D)culture conditions. Expansion of the hydrogel through temperature change promoted effective deliveryof ASCs to the site of injury, and viability was maintained after transferring ASCs to the target substrate. Treatment with the ASC spheroid sheet accelerated wound closure and regeneration of the stratifiedmulti-layered epithelium. The regenerated epithelium not only had structural integrity, but alsodisplayed phenotypic polarization across the mucosa; basal epithelial cells expressed cytokeratin 5,whereas cytokeratin 13-positive cells were located in the supra-basal layers. In addition, by day 7 aftertreatment, the mucosa that received the ASC spheroid sheet had significantly decreased inflammation,similar to that of the normal mucosa. These results demonstrate that the ASC spheroid sheet is aneffective treatment for ulcers in the oral cavity.

Tranilast-loaded tubular scaffold and surgical suture for suppression of stenosis after tracheal prosthesis transplantation

최지숙,Beom Kang Huh,Sang Jin Lee,Mi-Jung Han,Min Rye Eom,Hee-Jin Ahn,Young-Ju Jin,Su A Park,Young Bin Choy,Seong Keun Kwon 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.82 No.-

Most tracheal prosthesis implantation fail because of restenosis at the anastomotic site. Tranilast, whichis approved as an anti-allergic drug, has been found to inhibit the proliferation offibroblasts. In this study,use of a tracheal scaffold and surgical suture loaded with tranilast was proposed. A three-dimensionallyprinted tubular scaffold was designed as an artificial tracheal implant, both ends of which werefixed withsurgical sutures. The formation of granulation tissue at both ends of the scaffold were compared amongfour animal groups that were transplanted with a drug-loaded or bare scaffold and sutured with drugloadedor bare thread. In animal models with a tracheal defect, the inhibitory effect of tranilast onstenosis at an anastomotic site was observed to be synergistically enhanced when both the scaffold andsuture were loaded with tranilast, allowing for better survival after tracheal implantation. Histologicevaluation also revealed that more M2 phenotype macrophages, which are known to be responsible foranti-inflammation and tissue regeneration, were recruited around the scaffold than pro-inflammatoryM1 macrophages that otherwise would stimulatefibrosis. Therefore, these results suggest that trachealimplantation using a tubular scaffold and suture, both loaded with tranilast, is a promising strategy forsuppression of tracheal stenosis.