IRE1α protects against osteoarthritis by regulating progranulin-dependent XBP1 splicing and collagen homeostasis

Liang Li,Zhang Fengmei,Feng Naibo,Kuang Biao,Fan Mengtian,Chen Cheng,Pan Yiming,Zhou Pengfei,Geng Nana,Li Xingyue,Xian Menglin,Deng Lin,Li Xiaoli,Kuang Liang,Luo Fengtao,Tan Qiaoyan,Xie Yangli,Guo Fen 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Osteoarthritis (OA) is a full-joint, multifactorial, degenerative and inflammatory disease that seriously affects the quality of life of patients due to its disabling and pain-causing properties. ER stress has been reported to be closely related to the progression of OA. The inositol-requiring enzyme 1α/X-box-binding protein-1 spliced (IRE1α/XBP1s) pathway, which is highly expressed in the chondrocytes of OA patients, promotes the degradation and refolding of abnormal proteins during ER stress and maintains the stability of the ER environment of chondrocytes, but its function and the underlying mechanisms of how it contributes to the progression of OA remain unclear. This study investigates the role of IRE1α/ERN1 in OA. Specific deficiency of ERN1 in chondrocytes spontaneously resulted in OA-like cartilage destruction and accelerated OA progression in a surgically induced arthritis model. Local delivery of AdERN1 relieved degradation of the cartilage matrix and prevented OA development in an ACLT-mediated model. Mechanistically, progranulin (PGRN), an intracellular chaperone, binds to IRE1α, promoting its phosphorylation and splicing of XBP1u to generate XBP1s. XBP1s protects articular cartilage through TNF-α/ERK1/2 signaling and further maintains collagen homeostasis by regulating type II collagen expression. The chondroprotective effect of IRE1α/ERN1 is dependent on PGRN and XBP1s splicing. ERN1 deficiency accelerated cartilage degeneration in OA by reducing PGRN expression and XBP1s splicing, subsequently decreasing collagen II expression and triggering collagen structural abnormalities and an imbalance in collagen homeostasis. This study provides new insights into OA pathogenesis and the UPR and suggests that IRE1α/ERN1 may serve as a potential target for the treatment of joint degenerative diseases, including OA.

Expression and Characterization of RNA-dependent RNA Polymerase of Dendrolimus punctatus Tetravirus

Zhou, Liang,Zhang, Jiamin,Wang, Xiaochun,Jiang, Hong,Yi, Fuming,Hu, Yuanyang 생화학분자생물학회 2006 Journal of biochemistry and molecular biology Vol.39 No.5

Dendrolimus punctatus tetravirus (DpTV) has been identified as a new member of the genus Omegatetravirus of the family Tetraviridae that may be related serologically to Nudaurelia capensis virus ($N{\omega}V$). To establish the function of DpTV RNA genome and to better understand the mechanism of viral replication, the putative RNA-dependent RNA polymerase (RdRp) domain has been cloned and expressed in Escherichia coli. The recombinant protein was purified on a Ni-chelating HisTrap affinity column and demonstrated to initiate viral RNA synthesis in a primer-independent manner but not by terminal nucleotidyle transferase activity in the presence of $Mg^{2+}$ and RNA template. Mutation of the GDD to GAA interferes with the residues at the polymerase active site and metal ions, and thus renders the polymerase inactive.

Expression and Characterization of RNA-dependent RNA Polymerase of Dendrolimus punctatus Tetravirus

( Zhou Liang ),( Jia Min Zhang ),( Xiao Chun Wang ),( Hong Jiang ),( Fu Ming Yi ),( Yuan Yang Hu ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.5

HOCl Oxidation-modified CT26 Cell Vaccine Inhibits Colon Tumor Growth in a Mouse Model

Zhou, Rui,Huang, Wen-Jun,Ma, Cong,Zhou, Yan,Yao, Yu-Qin,Wang, Yu-Xi,Gou, Lan-Tu,Yi, Chen,Yang, Jin-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Despite progress in elucidating mechanisms associated with colorectal cancer and improvement of treatment methods, it remains a frequent cause of death worldwide. New and more effective therapies are therefore urgently needed. Recent studies have shown that immunogenicity of whole ovarian tumor cells and subsequent T cell response were potentiated by oxidation modification with hypochlorous acid (HOCl) in vitro and ex vivo. These results prompted us to investigate the protective antitumor response with an HOCl treated CT26 colorectal cancer cell vaccine in an in vivo mouse model. Administration of HOCl modified vaccine triggered robust antitumor immunity to autologous tumor cells in mice and prolonged survival period significantly. In addition, increased necrosis and apoptosis were found in tumor tissue from the oxidation group. Interestingly, ELISPOT assays showed that specific T cell responses were not elicited in response to the immunizing cellular antigen, in contrast to raising sera antibody titer and antibody binding activity shown by ELISA assay and flow cytometry. Further evaluation of the mechanisms underlying HOCl modified vaccine mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results combined with previous studies suggest that HOCl oxidation modified whole cell vaccine has wide applicability as a cancer vaccine because it can target both T cell- and B cell-specific responses. It may thus represent a promising approach for the immunotherapy of colorectal cancer.

Mechanical behavior of steel tube encased high-strength concrete composite walls under constant axial load and cyclically increasing lateral load: Experimental investigation and modeling

Liang Bai,Huilin Wei,Bin Wang,Fangfang Liao,Tianhua Zhou,Xing-Wen Liang 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.1

This paper presented an investigation into steel tubes encased high-strength concrete (STHC) composite walls, wherein steel tubes were embedded at the boundary elements of high-strength concrete walls. A series of cyclic loading tests was conducted to evaluate the failure pattern, hysteresis characteristics, load-bearing capacity, deformability, and strain distribution of STHC composite walls. The test results demonstrated that the bearing capacity and ductility of the STHC composite walls improved with the embedding of steel tubes at the boundary elements. An analytical method was then established to predict the flexural bearing capacity of the STHC composite walls, and the calculated results agreed well with the experimental values, with errors of less than 10%. Finally, a finite element modeling (FEM) was developed via the OpenSees program to analyze the mechanical performance of the STHC composite wall. The FEM was validated through test results; additionally, the influences of the axial load ratio, steel tube strength, and shear-span ratio on the mechanical properties of STHC composite walls were comprehensively investigated.

Robust Channel Estimation for OFDM based WLANs

Liang Zhou,Miyoshi Saito 대한전자공학회 2004 ITC-CSCC :International Technical Conference on Ci Vol.2004 No.7

Removal of Chromium (Ⅵ) by Escherichia coli Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study

( Xiaofeng Zhou ),( Jianghui Li ),( Weilong Wang ),( Fan Yang ),( Bingqian Fan ),( Chenlu Zhang ),( Xiaojun Ren ),( Feng Liang ),( Rong Cheng ),( Fengying Jiang ),( Huaibin Zhou ),( Juanjuan Yang ),( 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7

Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorptiondesorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.

《春秋》的记事特征及其对《左传》、《国语》的影响

李洲良(Zhou-Liang Li) 중국어문논역학회 2014 中國語文論譯叢刊 Vol.0 No.35

Whether the documents of our times or the literature unearthed, we have not found out the record feature of sublime words with deep meaning in Chuen-Qiou in accordance with any material in the pre-Qin period by far. In spite of the following objective conditions such as the troublesome words and strenuous writing instruments are trapped, carapare-bone and copper ware scripts have the style of contracted writings, which has to be simple in order to meet the need of abridged words. The author of Chuen-Qiou has omitted the origin, course and many details in accidents, cutdown the dialogues, speech of characters and interior presentation so as to the abundant vivid historical narrative is evacuated and drained for the descendants to recover. Although the record feature of sublime words with deep meaning has the root with the simple style of carapare-bone and copper ware scripts, which are on purpose in need of profound truth contained in subtle words. The writing of simple diction and appraised meaning formed by record feature in Chuen-Qiou has a direct effect on narrative in tso chuan and Mandarin, as turns in to the basic style of Chinese anciant narrative, that is, Chuen-Qiou writing style.

Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing

Chaoyue Liang,Zhuolin Wu,Xiaohong Gan,Yuanbin Liu,You You,Chenxian Liu,Chengzhi Zhou,Ying Liang,Haiyun Mo,Allen M. Chen,Jiexia Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.1

Purpose: This study aimed to identify potential epidermal growth factor receptor (EGFR) gene mutations in non-small cell lung cancer that went undetected by amplification refractory mutation system-Scorpion real-time PCR (ARMS-PCR). Materials and Methods: A total of 200 specimens were obtained from the First Affiliated Hospital of Guangzhou Medical University from August 2014 to August 2015. In total, 100 ARMS-negative and 100 ARMS-positive specimens were evaluated for EGFR gene mutations by Sanger sequencing. The methodology and sensitivity of each method and the outcomes of EGFR-tyrosine kinase inhibitor (TKI) therapy were analyzed. Results: Among the 100 ARMS-PCR-positive samples, 90 were positive by Sanger sequencing, while 10 cases were considered negative, because the mutation abundance was less than 10%. Among the 100 negative cases, three were positive for a rare EGFR mutation by Sanger sequencing. In the curative effect analysis of EGFR-TKIs, the progression-free survival (PFS) analysis based on ARMS and Sanger sequencing results showed no difference. However, the PFS of patients with a high abundance of EGFR mutationwas 12.4 months [95% confidence interval (CI), 11.6−12.4 months], which was significantly higher than that of patients with a low abundance of mutations detected by Sanger sequencing (95% CI, 10.7−11.3 months) (p<0.001). Conclusion: The ARMS method demonstrated higher sensitivity than Sanger sequencing, but was prone to missing mutations due to primer design. Sanger sequencing was able to detect rare EGFR mutations and deemed applicable for confirming EGFR status. A clinical trial evaluating the efficacy of EGFR-TKIs in patients with rare EGFR mutations is needed.

Method and application of spatial positioning for valid temperature-measuring optical fibers in concrete dams

Huawei Zhou,Chunju Zhao,Zhipeng Liang,Yihong Zhou,Fang Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

Spatial positioning of Valid Temperature-measuring Optical Fiber (VTOF) is of great significancefor accurately grasping the real temperature distribution of a concrete dam. However, there is barely an effective way to accurately locate the measuring points of optical fiber in concrete dams. A comprehensive data symmetry analysis technique is presented for determining the optimal spatial positioning of valid temperature-measuring optical fibers (VTOFs) in concrete dams. This approach includes a mathematical model to confirm the center of symmetry in monitoring data, which is required to reconstruct the real spatial temperature distribution. This process can identify VTOF locations and verify the reliability of temperature predictions. Experimental validation was conducted using findings from research on a new mathematical model, site measurements from a concrete dam, and corresponding data from distributed optical fibers. These results were successfully applied to the construction of the Baihetan dam; the concrete temperature from 405 pouring blocks in 5 typical dam monoliths was accurately monitored, and the temperature-measuring points of VTOFs were more than 24711. Meanwhile, the optical fiber temperature measurement and temperature field analysis system of Baihetan dam located in southwest China was developed based on accurately acquired temperature measurement data, which provides data basis and engineering application platform support for temperature evolution and temperature field analysis and precise temperature control management of pouring blocks.