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- 저자 : Zhuolin Wu (검색결과 3 건)
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Fuzzy Keyword Search Method over Ciphertexts supporting Access Control
( Zhuolin Mei ),( Bin Wu ),( Shengli Tian ),( Yonghui Ruan ),( Zongmin Cui ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.11
With the rapid development of cloud computing, more and more data owners are motivated to outsource their data to cloud for various benefits. Due to serious privacy concerns, sensitive data should be encrypted before being outsourced to the cloud. However, this results that effective data utilization becomes a very challenging task, such as keyword search over ciphertexts. Although many searchable encryption methods have been proposed, they only support exact keyword search. Thus, misspelled keywords in the query will result in wrong or no matching. Very recently, a few methods extends the search capability to fuzzy keyword search. Some of them may result in inaccurate search results. The other methods need very large indexes which inevitably lead to low search efficiency. Additionally, the above fuzzy keyword search methods do not support access control. In our paper, we propose a searchable encryption method which achieves fuzzy search and access control through algorithm design and Ciphertext-Policy Attribute-based Encryption (CP-ABE). In our method, the index is small and the search results are accurate. We present word pattern which can be used to balance the search efficiency and privacy. Finally, we conduct extensive experiments and analyze the security of the proposed method.
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Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing
Chaoyue Liang,Zhuolin Wu,Xiaohong Gan,Yuanbin Liu,You You,Chenxian Liu,Chengzhi Zhou,Ying Liang,Haiyun Mo,Allen M. Chen,Jiexia Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.1
Purpose: This study aimed to identify potential epidermal growth factor receptor (EGFR) gene mutations in non-small cell lung cancer that went undetected by amplification refractory mutation system-Scorpion real-time PCR (ARMS-PCR). Materials and Methods: A total of 200 specimens were obtained from the First Affiliated Hospital of Guangzhou Medical University from August 2014 to August 2015. In total, 100 ARMS-negative and 100 ARMS-positive specimens were evaluated for EGFR gene mutations by Sanger sequencing. The methodology and sensitivity of each method and the outcomes of EGFR-tyrosine kinase inhibitor (TKI) therapy were analyzed. Results: Among the 100 ARMS-PCR-positive samples, 90 were positive by Sanger sequencing, while 10 cases were considered negative, because the mutation abundance was less than 10%. Among the 100 negative cases, three were positive for a rare EGFR mutation by Sanger sequencing. In the curative effect analysis of EGFR-TKIs, the progression-free survival (PFS) analysis based on ARMS and Sanger sequencing results showed no difference. However, the PFS of patients with a high abundance of EGFR mutationwas 12.4 months [95% confidence interval (CI), 11.6−12.4 months], which was significantly higher than that of patients with a low abundance of mutations detected by Sanger sequencing (95% CI, 10.7−11.3 months) (p<0.001). Conclusion: The ARMS method demonstrated higher sensitivity than Sanger sequencing, but was prone to missing mutations due to primer design. Sanger sequencing was able to detect rare EGFR mutations and deemed applicable for confirming EGFR status. A clinical trial evaluating the efficacy of EGFR-TKIs in patients with rare EGFR mutations is needed.
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Zhou Jie,Xu Min,Chen ZhaoZhao,Huang LinLin,Wu ZhuoLin,Huang ZhongPei,Liu Lin 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.7
BACKGROUND: This study aims to explore the potential mechanism of action of the newly discovered hsa_- circ_0128899 (circSPEF2) in diffuse large B-cell lymphoma (DLBCL). METHODS: circSPEF2, miR-16-5p and BTB and CNC homologue 2 (BACH2) expression patterns in DLBCL patients and cell lines were studied by RT-qPCR. The biological function of circSPEF2 in vitro and in vivo was investigated by function acquisition experiments. The proliferation activity of lymphoma cells was detected by MTT. Bax, Caspase-3, and Bcl-2 were determined by Western Blot. Apoptosis and the ratio of CD4 to Treg of immune cells in the co-culture system were analyzed by flow cytometry. The mechanism of action of circSPEF2 in DLBCL progression was further investigated by RIP and dual luciferase reporter experiments. RESULTS: circSPEF2 was a circRNA with abnormally down-regulated expression in DLBCL. Increasing circSPEF2 expression inhibited the proliferative activity and induced apoptosis of lymphoma cells in vitro and in vivo, as well as increased CD4?T cells and decreased Treg cell proportion of immune cells in the tumor microenvironment. Mechanically, circSPEF2 was bound to miR-16-5p expression, while BACH2 was targeted by miR-16-5p. circSPEF2 overexpressionmediated effects on lymphoma progression were reversible by upregulating miR-16-5p or downregulating BACH2. CONCLUSIONS: circSPEF2 can influence DLBCL progression by managing cellular proliferation and apoptosis and the proportion of immune cells Treg and CD4 through the miR-16-5p/BACH2 axis.
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