Enterobacter aerogenes ZDY01 Attenuates Choline-Induced Trimethylamine N-Oxide Levels by Remodeling Gut Microbiota in Mice

( Liang Qiu ),( Dong Yang ),( Xueying Tao ),( Jun Yu ),( Hua Xiong ),( Hua Wei ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.8

Trimethylamine N-oxide (TMAO), which is transformed from trimethylamine (TMA) through hepatic flavin-containing monooxygenases, can promote atherosclerosis. TMA is produced from dietary carnitine, phosphatidylcholine, and choline via the gut microbes. Previous works have shown that some small molecules, such as allicin, resveratrol, and 3,3-dimethyl-1- butanol, are used to reduce circulating TMAO levels. However, the use of bacteria as an effective therapy to reduce TMAO levels has not been reported. In the present study, 82 isolates were screened from healthy Chinese fecal samples on a basal salt medium supplemented with TMA as the sole carbon source. The isolates belonged to the family Enterobacteriaceae, particularly to genera Klebsiella, Escherichia, Cronobacter, and Enterobacter. Serum TMAO and cecal TMA levels were significantly decreased in choline-fed mice treated with Enterobacter aerogenes ZDY01 compared with those in choline-fed mice treated with phosphate-buffered saline. The proportions of Bacteroidales family S24-7 were significantly increased, whereas the proportions of Helicobacteraceae and Prevotellaceae were significantly decreased through the administration of E. aerogenes ZDY01. Results indicated that the use of probiotics to act directly on the TMA in the gut might be an alternative approach to reduce serum TMAO levels and to prevent the development of atherosclerosis and “fish odor syndrome” through the effect of TMA on the gut microbiota.

Nonlegumes Respond to Rhizobial Nod Factors by Suppressing the Innate Immune Response

Liang, Yan,Cao, Yangrong,Tanaka, Kiwamu,Thibivilliers, Sandra,Wan, Jinrong,Choi, Jeongmin,Kang, Chang ho,Qiu, Jing,Stacey, Gary American Association for the Advancement of Scienc 2013 Science Vol.341 No.6152

<P><B>Stealth Nod Factor Recognition</B></P><P>Legumes' symbiotic interaction with nitrogen fixing bacteria supplies the plant with nitrogen. Many important crop plants, however, cannot establish these symbioses and, thus, agriculture depends on externally applied fertilizers. Surprisingly, <B>Liang <I>et al.</I></B> (p. 1384, published online 5 September) found that several nonleguminous plants, including <I>Arabidopsis</I>, tomato, and corn, were able to respond to the same Nod factors that initiate the microbial symbiosis in soybean.</P>

종역학간종교학술적정통여이단론쟁(從易學看宗敎學術的正統與異端論爭) -기력사성화현실성(其曆史性和現實性)

구량휘 ( Liang Hui Qiu ) 율곡학회 2010 율곡학연구 Vol.21 No.-

주역의 음양 변화 법칙에서 출발하여 기독교 문화 현상에 나타난 정통과 이단의 논쟁문제를 해독하고, 그 역사성과 현실성을 탐구 토론했다. Base on the principle of Yin and Yang changes in I-Ching,we interpret the debate appearing orthodoxy and heresy of Christian culture phenomenon, and discuss its history and reality.

關于現代易學的思考

구량휘 ( Qiu Liang-hui ) 동양사회사상학회 2008 사회사상과 문화 Vol.17 No.-

역학은 중화문화의 근본이다. 고대에는 ‘하역연산夏易連山’, ‘상역귀장商易歸藏’, ‘주역경전周易經傳’이 있었다. 역대역학에는 또한 상象, 수數, 의義, 리理의 각 학파가 있었다. 당대에 들어서는 자각적이든 그렇지 않든 모두 현대역학문화를 건설하고자 한다. 본문은 현대역학의 전개과정을 반성해 보고 향후 역학이 어떤 방향으로 진행되어야 하는가를 탐색해 보고자 한다. 그래서 본 연구는 기존의 역학에 대한 관리적 해석, 건축학적 해석, 인생론적 해석, 의료양생학, 인생철학에 대한 연구를 통해 현대 역학의 발전, 특성, 내함과 독창성에 대해 탐색하고 현대 역학 연구에 관한 토론 자료를 제공하고자 한다. 미래역학의 연구는 인문이념과 자연정신의 관점에서 주역의 긍정적인 측면과 부정적인 측면을 가려내어 긍정적인 측면을 계승 발전시켜야 할 것이다. 그리고 이 연구는 또한 현대 서양과학이 안고 있는 문제점을 해결하여 새로운 과학이론을 창출할 수도 있어야 할 것이다. 그리고 주역문화와 과학문화를 유기적으로 결합시켜 21세기 새로운 문화를 창조해야 할 것이다. I-Ching is the foundation of Chinese culture. After ancient Lianshan of Xia Dynasty, Guizang of Shang Dynasty, Jingzhuan of Zhou Dynasty and all previous dynasties, I-Ching developed different academic system, for example Xiang, Shu, Yi and Li. We consciously and unconsciously establish and develop modern I-Ching in present age. This paper proposes the opinion of characteristic, content and innovation of modern I-Ching. It is the preliminary summary of modern I-Ching.

Network Traffic Prediction Based on SVR Improved By Chaos Theory and Ant Colony Optimization

Yonglin Liang,Lirong Qiu 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.1

Network traffic prediction is one of the significant issues. The model for network traffic prediction should meet the following requirements. First, the model should be taken into consideration the characteristics of the network flow such as burstiness, long-range dependence, periodicity and self-similarity. To achieve this, we decompose the original flow in a multi-scale manner into a set of linear and stable representations, and introduce chaos theory to improve the diversity and search coverage. Second, the model should be efficient and accurate. To this end, we propose a prediction model based on SVR, and utilize Ant Colony Optimization (ACO) algorithm for parameter selection of SVR. Besides, we conduct experiments to evaluate the proposed model.

关于现代易学的思考

丘亮?(Qiu Liang-Hui) 동양사회사상학회 2008 사회사상과 문화 Vol.17 No.-

  역학은 중화문화의 근본이다. 고대에는 ‘하역연산夏易連山’, ‘상역귀장商易歸藏’, ‘주역경전周易經傳’이 있었다. 역대역학에는 또한 상象, 수數, 의義, 리理의 각 학파가 있었다. 당대에 들어서는 자각적이든 그렇지 않든 모두 현대역학문화를 건설하고자 한다. 본문은 현대역학의 전개과정을 반성해 보고 향후 역학이 어떤 방향으로 진행되어야 하는가를 탐색해 보고자 한다. 그래서 본 연구는 기존의 역학에 대한 관리적 해석, 건축학적 해석, 인생론적 해석, 의료양생학, 인생철학에 대한 연구를 통해 현대 역학의 발전, 특성, 내함과 독창성에 대해 탐색하고 현대 역학 연구에 관한 토론 자료를 제공하고자 한다. 미래역학의 연구는 인문이념과 자연정신의 관점에서 주역의 긍정적인 측면과 부정적인 측면을 가려내어 긍정적인 측면을 계승 발전시켜야 할 것이다. 그리고 이 연구는 또한 현대 서양과학이 안고 있는 문제점을 해결하여 새로운 과학이론을 창출할 수도 있어야 할 것이다. 그리고 주역문화와 과학문화를 유기적으로 결합시켜 21세기 새로운 문화를 창조해야 할 것이다.   I-Ching is the foundation of Chinese culture. After ancient Lianshan of Xia Dynasty, Guizang of Shang Dynasty, Jingzhuan of Zhou Dynasty and all previous dynasties, I-Ching developed different academic system, for example Xiang, Shu, Yi and Li. We consciously and unconsciously establish and develop modern I-Ching in present age. This paper proposes the opinion of characteristic, content and innovation of modern I-Ching. It is the preliminary summary of modern I-Ching.

Ganoderma Lucidum Polysaccharides Target a Fas/Caspase Dependent Pathway to Induce Apoptosis in Human Colon Cancer Cells

Liang, Zengenni,Guo, Yu-Tong,Yi, You-Jin,Wang, Ren-Cai,Hu, Qiu-Long,Xiong, Xing-Yao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Ganoderma lucidum polysaccharides (GLP) extracted from Ganoderma lucidum have been shown to induce cell death in some kinds of cancer cells. This study investigated the cytotoxic and apoptotic effect of GLP on HCT-116 human colon cancer cells and the molecular mechanisms involved. Cell proliferation, cell migration, lactate dehydrogenase (LDH) levels and intracellular free calcium levels ($[Ca^{2+}]i$) were determined by MTT, wound-healing, LDH release and fluorescence assays, respectively. Cell apoptosis was observed by scanning and transmission electron microscopy. For the mechanism studies, caspase-8 activation, and Fas and caspase-3 expression were evaluated. Treatment of HCT-116 cells with various concentrations of GLP (0.625-5 mg/mL) resulted in a significant decrease in cell viability (P< 0.01). This study showed that the antitumor activity of GLP was related to cell migration inhibition, cell morphology changes, intracellular $Ca^{2+}$ elevation and LDH release. Also, increase in the levels of caspase-8 activity was involved in GLP-induced apoptosis. Western blotting indicated that Fas and caspase-3 protein expression was up-regulated after exposure to GLP. This investigation demonstrated for the first time that GLP shows prominent anticancer activities against the HCT-116 human colon cancer cell line through triggering intracellular calcium release and the death receptor pathway.

Pretreatment Serum Amyloid A and C-reactive Protein Comparing with Epstein-Barr Virus DNA as Prognostic Indicators in Patients with Nasopharyngeal Carcinoma: A Prospective Study

Qiu-Yan Chen,Qing-Nan Tang,Lin-Quan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Chao-Feng Li,Yang Li,Yu-Jing Liang,Xue-Song Sun,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Yu-Ying Fan,Yan He,Ming-Yuan C 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose The measuring Epstein-Barr virus (EBV) DNA is an important predictor of nasopharyngeal carcinoma (NPC). This study evaluated the predictive value of pretreatment serum amyloid A (SAA) and C-reactive protein (CRP) comparing with EBV DNA in patients with NPC. Materials and Methods In an observational study of 419 non-metastatic NPC patients, we prospectively evaluated the prognostic effects of pretreatment SAA, CRP, and EBV DNA on survival. The primary endpoint was progress-free survival (PFS). Results The median level of SAA and CRP was 4.28 mg/L and 1.88 mg/L, respectively. For the high- SAA group (> 4.28 mg/L) versus the low-SAA ( 4.28 mg/L) group and the high-CRP group (> 1.88 mg/L) versus the low-CRP ( 1.88 mg/L) group, the 5-year PFS was 64.5% versus 73.1% (p=0.013) and 65.2% versus 73.3% (p=0.064), respectively. EBV DNA detection showed a superior predictive result, the 5-year PFS in the EBV DNA  1,500 copies/mL group was obviously different than the EBV DNA < 1,500 copies/mL group (62.2% versus 77.8%, p < 0.001). Multifactorial Cox regression analysis confirmed that in the PFS, the independent prognostic factors were including EBV DNA (hazard ratio [HR], 1.788; p=0.009), tumour stage (HR, 1.903; p=0.021), and node stage (HR, 1.498; p=0.049), but the SAA and CRP were not included in the independent prognostic factors. Conclusion The results of SAA and CRP had a certain relationship with the prognosis of NPC, and the prognosis of patients with high level of SAA and CRP were poor. However, the predictive ability of SAA and CRP was lower than that of EBV DNA.

Analysis of Multi-Harmonic Magnetic Field Coupling Characteristics of Bearingless Permanent Magnet Synchronous Motor

Qiu Hongbo,Chen Si,Liang Guangchuan,Yang Cunxiang 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.3

The suspension performance is an important evaluation index of the bearingless motor, which is the key to avoiding shutdown caused by stator and rotor collisions, as well as the safe and stable operation of the motor. In this paper, an Accurate Decoupling Mathematical Analysis Method for Multi-harmonic Magnetic Field Based on Maxwell Tensor Method. On the basis of the mathematical analysis model of the suspension performance and the analysis of the air gap multi-magnetic feld, the weight classifcation of the infuence of coupling magnetic feld on radial suspension force is studied. The spatial distribution and temporal variation of the suspension force and its fuctuation are analyzed according to the coupling relationship involving the constant, fuctuating components and the space-time vector in the space-time vector expression of the suspension force, and the relationship between the space–time coupled magnetic feld and the suspension performance is obtained. Further combined with numerical analysis, the efect of polar arc eccentricity on suspension performance is analyzed, the optimal eccentricity for suspension performance is found, and the efect of the harmonic magnetic feld of permanent magnets on the fuctuation of levitation force is verifed. Finally, the prototype is made, the experimental platform was built, and the experimental platform is constructed to test the static and the dynamic performance of the prototype.

Protective effect of liensinine on periodontitis through its antioxidant effect in mice

Liang Pang,Kai Zhu,Xia Feng,Weiwei Liu,Deguang Peng,Lihua Qiu,Xiang Gao,Jiang Deng,Ying Li,Xin Zhao 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

Liensinine is the most important functional compound in lotus seeds; its protective effect on periodontitis was determined and the mechanism of its antioxidant effect was also explained. During the experiment, we could observe that liensinine could reduce the gingival index in periodontitis-induced mice, and these effects were similar to that during the treatment of periodontitis with the metronidazole drug. After the treatment with liensinine, the serum Immunoglobulin G1 (IgG1) level was found to increase and IgG2a level was found to decrease compared to the periodontitis control mice. Liensinine and metronidazole could increase the superoxide dismutase (SOD) serum levels in the periodontitis-induced mice, and both the drugs at the same concentration (200 mg/kg) showed similar SOD activities, which was found to increase. Liensinine also increased the oxidationrelated factor of catalase (CAT), glutathione peroxidase, and decreased nitric oxide, malondialdehyde, and endothelin in the serum of mice, compared to the periodontitis control mice. The serum cytokine levels of Interleukin-6, tumor necrosis factor-a, and Interferon-c in liensinine-treated mice were lower than the control mice, but higher than the normal mice. The serum IL-4 cytokine level of liensinine-treated mice was reversed, i.e., higher than the control mice. In the periodontal tissue, the mRNA and protein expressions of inhibitor of kappa B-a, transforming growth factor beta 1, Interleukin-10, Mn-SOD, Gu/Zn-SOD, and CAT were increased by the treatment with liensinine compared to the control group mice, and nuclear factor kappa B expressions showed the opposite trend. Liensinine is a good functional compound in the treatment of periodontitis, similar to metronidazole drug. Higher concentration of liensinine had better effects.