Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Group Size And Measure Of Group-Level Properties

Kim,Kyoung-Su,Kim In-Sook,Jun,Gyung-Ju,Yang,Dong-Min 한국인적자원관리학회 2009 인적자원관리연구 Vol.16 No.4

Bliese와 Halverson(1998)은 집단수준의 효과를 분석하는데 있어서 기존에 사용되어 온 eta-squared 보다는 eta-squared 값이 사용되어야만 한다는 것을 주장하였다 그러나 이들이 제시한 수정된 eta-squared 접근은 다음과 같은 한계점을 가지게 되는 바 첫째, 이 접근법은 개인수준의 데이터를 집단 수준의 데이터로 만드는데 있어서의 정당성을 제공하는 기준을 구체적으로 제시하고 있지 못하다는 것을 들 수 있고, 둘째, 이 접근법은 또한 집단 내 효과를 용인하고 분석하는 기준을 제공하고 있지 못하다는 점을 들 수 있다 본 논문에서는 이러한 한계점을 극복할 수 있는 몇 가지의 대안을 제시하고 있으며, 이와 더불어 개인수준의 데이터를 집단 수준으로 만드는 것에 대한 정당성을 위한 또 하나의 추가적인 절차를 제시하였다 Bliese and Halverson(1998) argue that corrected eta-squared, rather than eta-squared, should be used for analyzing group-level effects However, their proposed corrected eta-squared approach has the following limitations (1) it fails to specify a criterion for justifying the aggregation of individual-level data to the group level and (2) it fails to specify a criterion for analyzing and allowing for within-group effects In this paper, we suggest several alternatives that overcome these limitations We suggest an additional procedure for justifying the aggregation of individual-level data to the group level

광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwanaju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.

남자 대학생의 음주 형태 변화에 영향을 미치는 유전적, 심리사회적 요인

김시경,이수경,김민경,이상익 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.4

Objectives : This study aimed to determine the general predictive factors of change in drinking behavior and to provide materials for preventing drinking problems during early adulthood through examining genetic and psychosocial factors affecting the change of drinldng behavior in college students. Methods : The subjects were 101 male college students, apart of 534 students who had completed the previous study in 2000. In the present study as a 6-years follow up, we reassessed the drinking pattem and psychosocial variables and compared the results with previous data of the same subjects. To identify factors affecting the current drinking pattem, we used stepwise multiple regession and logistic regression analysis. Results : D allele (ALDH2) was found to reduce the degree of drinking and suppress problematic drinking, and C allele (TPH) had a suppression effect on problematic drinking. Drinking motive had a direct effect on the degree of drinking and Pro-blematic drinking. Negative cognitive expectancy had a direct effect on problematic drinking. Conelusion : Authors found some factors affecting the change of alcohol drinking behavior in college students and confirmed that there were hierarchies of significance among these factors. These may be applicable as variables for predicting drinking behavior in early adulthood.

Antiadhesive effect and safety of oxidized regenerated cellulose after thyroidectomy

Kyoung Sik Park,Kyu Eun Lee,Do Hoon Ku,Su-Jin Kim,Won Seo Park2,Hoon Yub Kim3,,Mi Ra Kwon1,Yeo-Kyu Youn1 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.6

Purpose: To evaluate the antiadhesive effects and safety of an oxidized regenerated cellulose (Interceed) after thyroidectomy. Methods: Seventy-six thyroidectomized patients were prospectively randomized into two groups with regard to the use of Interceed. We evaluated each group for their adhesive symptoms using four subjective and four objective items at the 2nd week, 3rd and 6th month after thyroidectomy. All patients were examined for vocal cord motility by indirect laryngoscope at each period. Results: Total adhesion scores at each postoperative follow-up period decreased with time, but were not significantly different in each group. The median score for swallowing discomfort for liquid was significantly lower in the Interceed group than in the control group 2 weeks after surgery. In addition, the severity of skin adhesion to the trachea was reduced in the Interceed group compared with the control group 6 months after surgery. During the study, there were no adverse effects or significant differences in postoperative complications between the groups. Conclusion: Interceed appeared to be safe and effective in improving neck discomfort at early postoperative periods and preventing skin adhesion to the trachea 6 months after thyroidectomy.

수도권 소재 산부인과의원의 제왕절개율과 관련 요인분석

김윤미,고수경 여성건강간호학회 2002 여성건강간호학회지 Vol.8 No.3

This study aims to find factors that affect variations in cesarean section frequency rates among OBGY clinics in Metropolitan areas. The factors include patient. medical supplier characteristics and economic factors. This study is a cross-sectional analysis using health insurance delivery claims from July to December 2000 and files of the NHIC(national health insurance corporation). Multiple regression was used to analyze the dependent variable of cesarean section frequency rate at each clinic. The results are as follows' Cesarean section frequency rate is increasing in proportion to the number of the following patients repeated caesarean section. disproportion. obstructed labour, fetal distress. emergency caesarean section and self-employed patients. There are geographic variations as well. Cesarean section frequency rates are higher in Inchon and Gyonggi province than in Seoul. The higher number of total delivery the clinic has. the lower rate of cesarean section it has. Clinics with high frequency rates in 1999 showed higher rates the next year. Further research is required to develop evidence based delivery modes and change strategies for increasing normal delivery and activating midwife clinics.

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

천은사의 이승휴 유적지 발굴기

金慶洙 중앙대학교 인문과학연구소 2001 人文學硏究 Vol.32 No.-

천은사는 삼척에 있는 절이다. 이 절은 백두 대간의 줄기인 두타산 자락에 있다. 절경 으로 이름난 두타산이 바다로 향해 뻗어 가다가 삼척의 미로면에 이르러 세 봉우리로 뭉쳐지는데 그 봉우리 아래쪽 양지 바른 곳에 위치하고 있다. 내가 이곳을 찾은 것은 1992년이었다. 그때 나는 울산의 처용 설화에 심취해 있었다. 그 중에서도 처용 설화에 나오는 망 해사의 존재에 대하여 사회사적 해석을 할 수 없을까 하고 고심하고 있었다. 절이란 불교포교만을 위해서 운용되는 것이 아니라는 생각이 삼국유사를 읽으면서 여러 차례 생각났기때문이었다.

3D Dental-CT를 이용한 상악 매복 과잉치의 진단 : 증례보고

김재곤,김수경,양연미,백병주,김성희 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

과잉치는 법랑기(enamel organ)가 치배형성을 지속하거나 과도한 세포증식을 함으로써 발생한다. 과잉치는 상악 전방부에서 종종 발견되며, 유치열기와 혼합치열기에서 다양한 문제를 야기한다. 과잉치는 형태에 따라서 보충치(supplemental tooth)와 흔적치(rudimental tooth)로 나눌 수 있다. 보충치는 정상 절치와 크기 및 형태가 유사하나, 흔적치는 비정삭적인 형태와 작은 크기를 갖고 있다. 보충치는 영구 측절치 부위에서 호발하며 정상 전치와 유사한 형태와 크기를 갖고 있으므로, 발치시 주의를 기울여야 한다. 본 3증례는 상악 전방부에 정상 전치 형태의 과잉치를 갖는 증례들이다. 모든 증례에서, 치근단, 교합, 파노라마 방사선 사진 등의 기존 방사선 사진 촬영 외에 3Dimensional Dental-Computed Tomography(3D Dental-CT)를 촬영하여 진단하였다. 기존 일반 방사선 사진들(conventional radiographs)과 3D Dental-CT를 비교시, 일반 방사선 사진들은 2차원적인 영상만을 제공하여 상의 해석에 있어서 술자의 주관이 개입되는 한계가 있다. 반면 3D Dental-CT는 과잉치의 정확한 위치와 형태에 대한 객관적인 정보를 제공하며, 좀 더 보존적인 수술을 시행하고, 수술 시간과 스트레스를 줄이는데 유용하다. 그러므로 일반 방사선 사진 촬영 후 과잉치의 위치가 불분명하고 인접 영구치의 손상이 의심되는 경우 3D Dental-CT를 이용한 진단이 고려된다. Supernumerary teeth are teeth which result from the continued budding of the enamel organ of the preceding tooth or from excessive proliferation of cells. They are most often found in the maxillary anterior region. And they can be responsible for a variey of irregularities in the primary and transitional dentition. There are two morphological types of supernumerary teeth: supplemental and rudimentary. Supplemental teeth have normal shape and size. In contrast, rudimental teeth have abnormal shape and smaller size. Supplemental supernumerary teeth are most common in permanent lateral incisor area. Its extration must be decided more carefully with differential diagnosis between normal teeth, because it has nomal shape and size. We reports 3 cases of the normal incisor shaped teeth in the maxillary anterior region. In all cases, we used the 3D Dental-CT as well as the conventionl plain film such as periapical, occlusal, and panoramic ridiograph. Consequently, 3D Dental-CT was valualble to figure out the exact position and morphology of supernumerary teeth, to do more conservative surgery, and to reduce surgery stress and time.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.