Establishment of immortalized human ovarian surface epithelial cells using the lentiviral system from Korea Gynecologic Cancer Bank

( Jinkyoung Kong ),( Hyunja Kwon ),( Eun Ju Lee ),( Ha-yeon Shin ),( Wookyeom Yang ),( Jae-hoon Kim ),( Doo Byung Chay ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: Immortalization is a key difference that distinguishes cancer cell form normal cell. In cell line studies, normal cells like human ovarian surface epithelial cells (HOSE) are difficult to culture and limited due to the senescence nature of normal cell. Thus for the expansion of biospecimen from Korea Gynecologic Cancer Bank, the purpose of this study was to establish immortalize human ovarian surface epithelial cells (IHOSE). 방법: Immortalize human ovarian surface epithelial cells (IHOSE) were established by transfecting HPV E6/E7 and SV40 T antigen to short cultured human ovarian surface epithelial cells (HOSE) using the lentiviral system. Cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) in the presence of 10% fetal bovine serum (FBS) and were cultured in 5% CO2 balanced air at 37°C. After confirmation of successful passages, immortalize human ovarian surface epithelial cells (IHOSE) were analyzed for alteration of gene expressions by DNA fingerprinting method. 결과: Five new human ovarian surface epithelial cells that succeeded ten passages were established using the lentiviral system (IHOSE). For molecular expression, 6% differential gene expression was noted compared to three human ovarian surface epithelial cells (HOSE). 결론: Newly established human ovarian surface epithelial cells, from Korea Gynecologic Cancer Bank, can be important research resources for a mimic model against molecular alternation of ovarian cancer.

The utility of 2-antibody panel using MSH6 and PMS2 for screening of Lynch syndrome in endometrial cancer patients

( Jisup Kim ),( Jinkyoung Kong ),( Soohyn Kim ),( Min Ae Cho ),( Doo Byung Chay ),( Soon Won Hong ),( Jae-hoon Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: Lynch syndrome (LS) is an autosomal dominant genetic condition which increases the risk of developing colorectal, endometrial, and various other cancers. Inefficiency of selective screening method based on personal/family history has led to general use of universal screening method with 4-antibody panel (MLH1, MSH2, MSH6, PMS2) for detecting Mismatch Repair (MMR) gene deficiency. For cost-effectiveness of universal Lynch screening the use of 2-antibody panel composed of MSH6 and PMS2 have been suggested in patients with colorectal cancer. The purpose of this study is to evaluate the immunohistochemisrty results of missmatch repair gene expression in endometrial cancer. 방법: 120 endometrial carcinoma specimens derived from patients who performed hysterectomy from 2009 to 2016 were stained with 4-antibody panel (MLH1, MSH2, MSH6, PMS2) 결과: Overall, 49 out of 120 cases (40.8%) showed loss expression in at least one MMR genes. 16 case (21.3%) showed loss of MLH1 and PMS2. 11case (9.2%) showed loss of MSH2 and MSH6. 11 case (9.2%) showed loss of MSH6. 1 case showed loss of PMS2 expression. There was no case showing isolated loss of MLH1 or MSH2. 결론: Our finding proves the utility of 2-antibody panel (MSH6, PMS2) in screening of Lynch syndrome in endometrial cancer. Considering the cost-effectiveness, 2-antibody panel should be implemented for universal screening for Lynch syndrome in endometrial cancer patients.

Controlled epitaxial growth modes of ZnO nanostructures using different substrate crystal planes

Hong, Young Joon,Yoo, Jinkyoung,Doh, Yong-Joo,Kang, Suk Hoon,Kong, Ki-jeong,Kim, Miyoung,Lee, Dong Ryeol,Oh, Kyu Hwan,Yi, Gyu-Chul Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.7

<P>A combined experimental and theoretical investigation has clarified the nanometre-scale vapour-phase epitaxial growth of ZnO nanostructures on different crystal planes of GaN substrates. Under typical growth conditions, ZnO nanorods grow perpendicular to the GaN(0001) plane, but thin flat films form on GaN(101&cmb.macr;1), (101&cmb.macr;0) and (112&cmb.macr;0). High-resolution X-ray diffraction data and transmission electron microscopy confirm the heteroepitaxial relationship between the ZnO nanostructures and GaN substrates. These results are consistent with first-principles theoretical calculations, indicating that the ZnO surface morphologies are mainly influenced by highly anisotropic GaN/ZnO interface energies. As a result of the large surface energy gradients, different ZnO nanostructures grow by preferential heteroepitaxial growth on different facets of regular GaN micropattern arrays. High-resolution transmission electron microscopy shows that ZnO nanotubes develop epitaxially on micropyramid tips, presumably as a result of enhanced nucleation and growth about the edges.</P> <P>Graphic Abstract</P><P>Combined experimental and theoretical investigations have clarified the controlled catalyst-free vapour-phase epitaxial growth mode of ZnO nanorods, nanotubes, and thin films on different crystal planes of GaN substrates. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b816034a'> </P>

Controlled epitaxial growth modes of ZnO nanostructures using different crystal planes

Young Joon Hong,Jinkyoung Yoo,Yong-Joo Doh,Suk Hoon Kang,Ki-jeong Kong,Miyoung Kim,Dong Ryeol Lee,Kyu Hwan Oh,Gyu-Chul Yi 한국진공학회 2009 한국진공학회 학술발표회초록집 Vol.36 No.-

GO-24 : Forkhead box P1 (Foxp1) in cervical cancer

( Hyeong Ju Kim ),( Ji Yun Lee ),( Jinkyoung Kong ),( Ji Hee Choi ),( Geum Seon Sohn ),( Eun Ji Nam ),( Sang Wun Kim ),( Doo Byung Chay ),( Sunghoon Kim ),( Jae Hoon Kim ),( Young Tae Kim ),( Han Byou 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: The forkhead box protein 1 (FOXP1) is considered as both a tumor suppressor candidate and a potential oncogene. Here, we investigated FOXP1 expression in cervical cancer, and the clinical significance of FOXP1 and it`s mechanism of action in cervical cancer. 방법: FOXP1`s functional role was investigated by employing lentiviral-mediated overexpression and knockdown in cervical cancer cell lines. Immunohistochemical staining for FOXP1 was performed on a cervical cancer tissue microarray consisting of 158 primary cervical cancers, 280 cervical intraepithelial neoplasias (CINs), and 378 matched normal tissues. 결과: FOXP1 overexpression promoted cell proliferation and tumorigenesis, whereas FOXP1 knockdown inhibited these properties in HeLa and CaSki cell lines. By immunohistochemical staining, FOXP1 expression increased during the normal to tumor transition of cervical carcinoma (p< 0.001), and this increased expression was significantly associated with tumor stage (p=0.009) and tumor grade (p< 0.001). In multivariate analysis, FOXP1+ (p=0.031) and tumor stage (p=0.032) were independent prognostic factors for overall survival. 결론: Taken together, our data indicate that FOXP1 has a crucial role in cervical cancer progression, and its overexpression is associated with poor prognosis, supporting that FOXP1 may be used as a promising novel target for therapeutic interventions.

GO-17 : Makorin Ring Finger Protein 1 (MKRN1) as an adjunct marker in liquid-based cervical cytology

( Ji Yun Lee ),( Hyeong Ju Kim ),( Jinkyoung Kong ),( Ji Hee Choi ),( Geum Seon Sohn ),( Hanbyoul Cho ),( Eun Ji Nam ),( Sang Wun Kim ),( Doo Byung Chay ),( Sunghoon Kim ),( Young Tae Kim ),( Jae Hoon 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: The aim of the study was to test the ability of MKRN1 to detect cervical lesion in a screening setting and its use as a surrogate marker of HPV infection. 방법: We conducted PROspective specimen collection and retrospective Blinded Evaluation (PROBE) study. Liquid-based cytology samples were collected from 187 women. A cell block was prepared from residual cervical cytology specimen for immunocytochemistry of MKRN1 and P16 INK4a.Liquid-based cervical cytology, MKRN1, P16 INK4a, P21, P57, KI-67 immunostaining on cell block sections, HPV hybrid capture and real time Polymerase chain reaction (PCR) were performed and analyzed with pathologic results.Clinical performances [sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV)] were calculated for all women and subgroup analysis was performed for patients with ASCUS or LSIL. 결과: MKRN1 positivity increased with histologic severity, from 32.4% in CIN1, 60.0% in CIN2, 80.0% in CIN3 to 92.3% in invasive cancer. The sensitivity, specificity, PPV and NPV of MKRN1 to detect CIN2+ was 73.8%, 76.8% 75.6%, and 75.0%, respectively. However, liquid-based cytology showed poorer clinical performance than MKRN1 immunostaining (61.3%, 69.5%, 66.2% and 64.8%, respectively) and HPV testing had lower specificity than MKRN1 (67.7%). When combining two screening tests, MKRN1 + HPV showed the highest sensitivity, specificity, PPV and NPV (71.8%, 85.5%, 82.3%, 76.5%, respectively), while there were no significant differences between cytology + HPV (60.6%, 81.8%, 75.4%, 69.2%) and cytology + MKRN1 (58.8%, 84.1%, 78.3%, 67.7%) in all aspects. In cases of ASCUS/LSIL, MKRN1 + HPV (100%, 72.7%, 73.9%, 100%, respectively) showed the best clinical performances followed by MKRN1 + cervical cytology (100%, 50.0%, 60.7%, 100%, respectively). 결론: MKRN1 was more sensitive and specific than liquid-based cytology, and it was more specific than HPV test. Our study showed that MKRN1 + HPV has the highest sensitivity and specificity when combining two diagnostic tests. As part of screening procedure, MKRN1 could be used as a satisfactory biomarker for the primary screening of cervical cytology.

GO-23 : Chemosensitivity testing based on gene expression profiling in patients with ovarian cancer

( Geum Seon Sohn ),( Hyeong Ju Kim ),( Ji Yun Lee ),( Jinkyoung Kong ),( Ji Hee Choi ),( Hanbyoul Cho ),( Eun Ji Nam ),( Sang Wun Kim ),( Sunghoon Kim ),( Jae Hoon Kim ),( Young Tae Kim ),( Doo Byung 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: To evaluate the association between clinical response of treatment agents and results of chemosensitivity testing in ovarian cancer. 방법: Tissue was obtained from 21 ovarian cancer patients and gene expression was evaluated by quantitative real-time polymerase chain reaction (PCR). Selected gene panel with expression of specific genes in the pathways that are related to drug responses in ovarian cancer were analyzed( AKT, Aurora A, BCRP, CD31, ERCC1, GSTpi, HER2, MDR1, Mitosin, PI3 Kinase, RRM1, Survivin, TOP1, TOP2A, TS, VEGF, VEGFR2, XIAP, P73). Gene expression were matched with therapeutic agent including Platinum, Taxanes, Bevacizumab, Gemcitabine, Topoisomerase I Inhibitors, Topoisomerase II Inhibitors, Cyclophosphamide, Herceptin, and 5-fluorouracil for chemosensitivity. 결과: Chemosensitivity testing revealed sensitivity rate of 66%, 81%, 96%, 56% and 61% for Platinum, Taxanes, Topoisomerase I Inhibitors, Topoisomerase II Inhibitors, and Bevacizumab, respectively. Treatment response rate was 70% (Complete Response: 40%, Partial Response: 30%). Treatment response was not significantly increased in the platinum sensitive patients (p=0.613), and overall response rate did not significantly differ according to the chemosensitivity test. 결론: This study may provide useful information in optimizing individual chemotherapy in the treatment of ovarian cancer.