Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

인접한 고층건물의 豊荷重 상호간섭에 관한 연구

조강표,정진택,정명채,김윤석 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Wind load is evaluated based on Standard Design Loads for Building of AIK(Architectural Institute of Korea), which was obtained from wind-tunnel experiment for an isolated building in open country, actual wind load can be different from the code because of terrain, building geometry, wind direction, separation distance between the buildings. Among them. separation distance is the most important factor. This paper presents the interference factor for sqaue section high-rise building structure, focusing on the separation distance. Interference factor was obtain from the force balance test in a wind tunnel.

Lipopolysaccharide로 유발된 생쥐 무릎 관절염 완화에 미치는 진통산의 효과 : 윤활관절막과 섬유관절막의 형태 변화를 중심으로 Based on the Morphological Change of Synovial Membrane and Fibrous Membrane

김진택,최홍식,안상현,박인식,강윤호,김호현,이해풍 동국대학교 경주대학 1999 東國論集 Vol.18 No.-

Knee joint BALB/C mice were administered with JINTONGSAN(JTS) extract after Lipopolysaccaride(LPS) injection were observed to investigate the anti-inflammatiory effect of JTS for arthritis. The JTS extract(3.3 ㎖/㎏/day) were daily administered to mice suffered from arthritis of knee joint induced by LPS injection, as dose of 300㎖/㎏. The specimen were fixed in 4% paraformaldehyde and were decalcificate in EDTA solution for 4 Weeks. In synovial membrane, the hyperplasia of synovial lining cell(fibroblast like synoviocytes) on JTS group were diminished then LPS group. The number of synovial lining cell(synovial phagocytic cells) with filopodia and the infiltrated of lymphocyte on JTS group were decreased. In fibrous membrane, The region of fibrosis on JTS group were mitigated than LPS group and the appearance of fibroblast and migrated cell, as neutrophil leukocytes and lymphocyte, were decreased. As results indicated that JTS was effective in anti-inflammatory reaction for LPS-induced arthritis.

剛體要素法으로 휨이 考慮된 쉘要素를 利用한 內壓容器의 彈塑性解析

權宅鎭,金勝德,朴剛根 成均館大學校 科學技術硏究所 1986 論文集 Vol.37 No.2

The Rigid Element Method(R.E.M) is a kind of the numerical discretization, techniques, such as the finite element method or the boundary element method, for getting the approximate solution of continuum mechanics problem. This method is originally developed for elasto-plastic analysis of wall-type precast reinforced concrete structures. In R.E.M., each discreted element is regarded as rigid body and the elements are interconnected with virtual springs with the stiffness of element. The springs transmit the axial force, the shear force and the normal force. The degree of freedom is placed in an arbitrary point within the element and the rigid displacements of this point affect other element through the springs being around the element. In this paper, it is derived the generalized adaptation matrix of shell elements which consider the effect of bending moment in thickness direction and developed a computer program using the present theory. Also, it is analyzed the behaviors of structure, that is, the distribution of stresses, the progress of cracks, the shape of deformation and the mode of fractures, for the hemispherical shell supported on cylinder under internal pressure.

뇌혈관질환을 동반한 당뇨병 환자에서 지속적 혈당측정의 유용성 평가

정성훈,강인구,박철영,류미숙,우정택,김성운,김진우,김영설 대한당뇨병학회 2002 임상당뇨병 Vol.3 No.2

연구배경: 뇌혈관질환을 동반한 고령의 당뇨병환자는 이들의 신경학적 결손으로 경고 증상없이 심한 저혈당에 노출될 가능성이 크다. 실제로 하루 4번 정도의 자가 혈당 측정으로는 이를 감지하기 어려울 뿐 아니라 검사에 따른 고통으로 적극적인 혈당 조절에 제한이 따르게 된다. 이에 저자들은 Mini Med사의 지속적 혈당측정기(CGMS, continuous glucose monitoring system)를 이용하여 뇌혈관질환을 동반한 당뇨병 환자에서 24시간 혈당의 변동양상을 알아보고 각기 다른 인슐린 치료방침에 따른 혈당조절의 효율성을 비교하고자 하였다. 방법: 2001년 1월부터 6월까지 경희대학교 부속병원에 입원해 있는 10명의 뇌혈관질환을 동반한 제 2형 당뇨병환자를 대상으로 하였다. 이 들은 모두 튜브식이를 받고 있엇고 최소 1개월 이상 인슐린 치료를 시행 받았다. 지속적 혈당측정기를 착용하고 3일간의 연속적인 혈당을 측정하였고, 치료 첫 날은 NPH, 둘째 날은 속효성 인슐린과 NPH, 마지막 날은 혼합형 인슐린제제로 치료하여 그 효과를 비교하였다. 이들 각기 다른 인슐린 제재의 하루 총량에 차이는 없었다. 혈당치가 3.3mmol/L 미만이거나 7.8mmol/L를 초과하는 경우 그 차이값의 총합을 시간으로 나누어 이 값(△Glu)이 적을수록 치료효과가 우수한 것으로 판정하였다. 또한 당 수치가 3.3mmol/L 미만인 경우를 저혈당 event, 16.7mmol/L 초과한 경우를 고혈당 event로 임의로 정의하였고, 이를 통해 지속적 혈당측정의 유용성을 간접적으로 평가하고자 하였다. 결과: 평균 △Glu값은 첫째 날이 0.93 ± 0.43mmol/Lㆍmin?¹, 둘째날이 0.71 ± 0.29mmol/Lㆍmin?¹ 마지막 날이 0.58 ± 0.29mmol/Lㆍmin?¹로서 서로 다른 3가지 치료방침사이에 통계학적으로 유의한 차이는 없었다(p=0.115). 10명의 환자들 중 자가 혈당측정기로는 1명에서 저혈당 event, 2명에서 고혈당 event를 인지하였으나 지속적 혈당측정기로는 각각 9명에서 event를 확인하였다. 결론: 지속적 혈당측정기를 이용한 각각의 인슐린 치료방침에 따른 혈당조절 효율성의 차이는 없었으나, 자가혈당 측정기로는 알 수 없었던 일 중 혈당 변동사항을 정확하게 알 수 있어, 뇌혈관질환을 동반한 당뇨병 환자에서 보다 적극적인 인슐린 치료 및 관리를 할 수 있을 것으로 생각된다. Background: Diabetic stroke patients are susceptible to hypoglycemia. However, there are many limitations in detecting hypoglycemic events, even though glucoses levels are checked 3 to 4 times per day using the fingerstick method. Therefore, we investigated the glycemic excursions and pattern in diabetic stroke patients using the continuous glucose monitoring system (CGMS, MiniMed) and its clinical utility. The other objective was to compare the treatment dfficacy between insulin regimens. Methods: From jan 2001 to jun 2001, 10 adult type 2 diabetic stroke patients wore CGMS for consecutive 3 days, which continuously checked the glucose level. NPH insulin, multiple daily injection of regular insulin ± NPH and the mixed type(Novolet 30/70, Novo Nordisk) were used on the first, second and third days of the study, respectively. If the mean delta glucose(△Glu = A+B/time, A=∑(hlucose-7.8), glucose > 7.8 mmol/l, B=∑(3.3-glucose) if, glucose < 3.3 mmol/l) was calculated in order to recognize the degree of individual glycemic changes and to compare the efficacy of each treatment diverse insulin regimen. We arbitrarily defined a glucose level less than 3.3mmol/l, as a hypoglycemic event, and more than 16.7mmol/l as a hyperglycemic event. Results: Variable patterns of glycemic changes were observed among patients with the same dosage, but a different insulin regimen. The mean △Glu was 0.93±0.43 on the first day, 0.71±0.29 on the second day and 0.58±0.29 on the third. There was no significant difference in treatment efficacy between the 3 different insulin modalities(p=0.115). Of the 10 patients, a hypoglycemic event was detected in 1 with the fingerstick method, in contrast to 9 with CGMS, with hyperglycemic events being detedted in 2 and 9, respectively. Conclusion: Continuous glucose monitoring maybe useful in providing the information necessary for optimal glycemic control in the diabetic stroke patients.

有限要蘇法에 依한 多孔板의 應力解析

呂白楡,權宅鎭,姜文明 啓明大學校 産業技術硏究所 1981 産業技術硏究所 論文報告集 Vol.3 No.-

The perforated elastic body have been dealt with the stress distribution along with stress con-centration, and boundary value. And not a few analytic studies were carried out and reported. Theoretically, the top-slab of nuclear reactor, a kind of perforated plate equipped with small hoes of regular arrangement, is too complicated to be analyzed except in particular forms of plates. In this study the boundary conditions and unit patterns of perforated plates are assumed as shown in figure 2-1 and figure 2-3. Then this unit pattern is divided into some triangular elements shown in figure 2-4. For the numerical analyses of the pattern a suitable program of F. E. M. is constructed using the stiffness matrix of Constant Strain Triangular Element. Using this program four models (ie A. B, C and D) are evaluated in this paper. And factors of stress concentration, states of stress distribution and effective elastic constants of these models are shown in graphic forms so that design of structure might be done easily. F. E. M. program of this paper may be regarded as useful to two-dimensional stress problems under the conditions shown in figure 2-3.

葉成分과 土壤의 理化學的性質이 林木生長에 미치는 영향

李光洙,鄭永觀,朴南昌,崔載彩,姜眞擇 경상대학교 농과대학 부속연습림 2001 演習林硏究報告 Vol.- No.11

This study was carried out to analyze physical and chemical properties of soil and leaf analysis to estimating amount of tree growth in Chamaecyparis pisifera, Jinhae city, Gyeongnam province. The results were summarize as follows; As the results from analysis of relation of physical-chemical properties of soil and leaf component, the relationship p component inside soil and n component inside leaf showed positive correlation with determination coefficients(R^2=0.9986), and also relation of nitrogen component inside soil and component of Na_2O inside leaf showed positive correlation with determination coefficients(R^2=0.9987), but the relation of component Ca^2+ inside soil and component of Na_2O inside leaf showed negative correlation with determination coefficients(R^2=-0.9984), and also relation of p component inside soil and Fe component inside leaf showed negative correlation with determination coefficients(R^2=-0.7215). The results from analysis of relation of leaf component and tree growth, showed correlation of determination coefficient(R^2=0.4256) in height growth, determination coefficient (R^2=0.6248) in DBH growth, determination coefficient(R^2=0.6248,) in volume growth, respectively. In each tree growth of recent five years, N and P components showed positive correlation of determination coefficient(R^2=0.6170, 0.5780, respectively) in height growth, and also N and Na^+ components showed positive correlation of determination coefficient(R^2=0.6016, R^2=0.8727, respectively) in DBH growth, but K= component showed negative correlation in DBH growth As the results from analysis of stepwise regression to estimating amount of DBH growth, sequence of inputted variables entered in order of N, Na+, Mg++, ,and estimated equation was Y=44.791N-56.640Mg+0.6867Na-26.47, determination coefficients of 69%.

Comparison of Seed Vigour Test by Several Methods in Pinus rigida x P. taeda

Jin-Taek Kang,Kyung-Hwan Jang,Byung-Sil Lee,Iin-Sik Kim,Tae-Su Kim,Tae-Dong Kim 한국종자연구회 2006 종자과학과 산업 Vol.3 No.1

Ribosomal Synthesis of Modified Histone H3 Tails

Taek Jin KANG 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.4