Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

全北地方의 藥用作物 재배 및 生産現況

郭晙洙,金昌洙,宋英恩,金柱,朴珍華,韓宗鉉,宋昊俊 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

본격적인 지방화 시대를 맞이하면서 지역특화작물의 개발과 농가소득증대를 위한 새로운 기술개발이 절실한 이때 주요 소득원작물의 하나인 약용작물의 재배 및 생산현황을 파악하여 지역특성에 맞는 새로운 작목개발 및 정책 입안 자료로 활용하고자 조사분석한 결과는 다음과 같다. 1. 생산량을 기준으로한 전북지방의 주요 약용작물을 특화계수 분석결과 총 31개작목 중 독활, 백지, 양유, 오미자, 지황 등은 핵심품목으로 길경, 두층, 방풍, 작약, 천마 등은 주산품목으로 조사되었으며 강활 등 21개 작목은 비특화 작물로 분석되었다. 재배면적을 기준으로 한결과는 독활, 백지, 양유, 지황 등이 핵심품목으로 길경, 두층, 목단, 방풍, 백출, 오미자, 의이인, 작약 등은 주산품목으로 강활 등 19개 품목은 비특화 작물로 분석되었다. 2. 주요 약용작물 재배 계획면적 조사에서는 당귀, 두층, 오미자, 독활, 천궁 등이 계획 대비 실제 재배면적의 차이가 큰 작목으로서 이들은 일반적으로 상품가격의 영향을 많이 받는 것으로 분석되었다. 3. '96 재배면적 조사 결과 전북지방의 주요 약용작물 재배 계획 면적은 총 1,692.7ha였으며 이중 독활이 426ha로 가장 많았고, 길경(283ha), 양유(179ha), 작약(165ha), 두층(154ha), 당귀(113ha), 오미자 의이인, 지황, 산수유, 천궁, 방풍 등의 순이었으며 기타작목으로 백지, 황기, 백하수오 등 28작목이 조사되었다. With the beginning of the new localization era, epoch-making techniques in local agriculture are needed to improve farm income and devlop a locally specialized crops. On the basis of these trends in local agriculture, the status quo of production in medicinal plants which are one of the major income crops were surveyed and analyzed the data for developing a new crops and drafting a policy. The major results are as follows; 1. The results analyzed by Location Quotient of production rate in Chonbuk were classified three catagories. 5 items including Aralia continentalis K_ITAGAWA among 31 items were so rted out as a core crops, another 5 items including Platycodon grandiflotum(J_ACQ.) A.DC. were as a main crops and other 21 items were as a non-specialized crops. Analyzed by cultivation area, 4 items were sorted out as a core crops, another 8 items were as a main crops and the other 19 items were as a non-specialized crops. 2. In comparison with the gap between intended cultivation area and real cultivation area, 5 items including Eucommia ulmoides OLIVER were showed more larger than the others. The above mentioned items were assumed to be more influenced by the price. 3. Farmer's intended cultivation areas of the major medidinal plants in 1996 were surveyed totals of 1.692 ha in Chonbuk. Among them, Aralia corolata was surveyed as the top totals of 426 ha. in area.

Time Marching 방법을 이용한 익렬내의 천이음속 유동해석

곽용민,이진호,조강래 연세대학교 산업기술연구소 1989 논문집 Vol.21 No.1

In this study, A computer program is developed for the analysis of transonic flow field, using Denton's improved time marching scheme. The results are compared with Ecer's results and those obtained by flow anylysis program of General Electric Co. From the comparison of these results, it was found that the developed program can be applied to the transonic flow field analysis.

유아 발달 수준 검사 개발 연구

곽노의,김진호,유구종 열린유아교육학회 1998 열린유아교육연구 Vol.3 No.1

This study is Research Task supported by National Education Assessment Institute in 1996. 「Developmental Level Test in Early Childhood」 is standardized assessment instrument examining validity and reliability developed by researchers. Subjects were 3,136 preschooler(age : 4.0∼7.0) sampled in National area. This assessment instrument consist of 77 items(for teacher) and 67 items(for parent). Data reveals that Construct Validity and Content Validity is high(p<.01). Data, employing factor analysis, reveals that a seven-factor solution is the best fit for the Developmental Level Test in Early Childhood. Cronbach α is .98(for teacher). This analysis indicates that This Instrument is acceptable for general use in Korea.

고속 주행 모드에서 자동차 디퓨저의 스트레이크 개수와 배치에 따른 공기저항 유동해석

이진호,박성하,최동석,한영진,곽나영,이상욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

This study proposes an optimized diffuser strake design that can improve driving performance for cars in high-speed driving mode. The speed of the vehicle is set to 160km/h, and the number of strake is set to 4, 6 and 8, and the angle of the diffuser is set to 4, 5 and 6 degrees. The arrangement of the strake is centered, edged and equally arranged. The optimized diffuser design induced with the Ansys Fluent analysis shows a drag coefficient of 16% less than the basic diffuser-free model at a diffuser angle of 6 degree, 8 strakes, and equal arrangement.

자폐장애 환자에서 FMR-1 유전 삼염기 반복의 분자생물학적 분석

곽호순,정철호,전효진,장은진,김희철,김정범,박영남 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.1

연구목적 : 자폐장애의 원인을 유전학적으로 규명하려는 연구가 시도되고 있으며, 그 중 fragile-X 증후군과의 연관성에 대한 연구가 활발히 진행되고 있다. fragile-X 염색체(Xq27.3)는 세포유전학적 방법으로 증명할 수 있으나 검사에 많은 제약과 단점이 있으므로, 본 연구에서는 보다 신뢰성이 높은 분자 생물학적 방법으로 FMR-1 유전자내 CGG 삼염기 반복부위를 분석하여 자폐장애와 fragile-X 증후군의 연관성을 규명하고자 하였다. 방법 : 자폐장애 환아(99명)와 정상대조군(8명)을 대상으로 FMR-1 유전자의 CGG 반복배열 부위를 sense와 antisense primer를 이용하여 PCR법으로 분석하엿으며, 동시에 세포유전학적 검사도 시행하였다. PCR 분석에서 CGG 반복수가 50 이상인 경우에 대해서는 StB12.3 혹은 Pfxa3 probe를 이용한 Sourthern blot hybridization으로 확인하였다. 결과 : FMR-1 유전자의 CGG 반복배열에 대한 PCR 분석 결과 CGG 삼염기의 반복배열의 수는 자폐장애 환자군과 정상대조군 사이에 통계적으로 유의한 차이가 없었다(p=0.207). 자폐장애 환자에서 CGG 반복수가 50회 이상인 조기변이(premutation) 환자가 2명 있었으나 Sourthern blot hybridization 결과 완전변이(full mutation)로 판정할 수 있는 경우는 없었다. 세포유전학적 검사에서 환자군 모두에서 정상 핵형을 나타내었으며 fragile-X 염색체는 확인되지 않았다. 결론 : 이상의 결과에서 자페장애 환자가 FMR-1 유전자의 CGG 삼염기 반복부위 이상, 즉 fragile-X 염색체 이상을 동반하지 않았음을 증명할 수 있었다. 이는 fragile-X 증후군을 자�장애의 직접적인 원인이라고 보기에는 어려움이 있음을 시사한다. Objectives : There has been a rapid expansion of studies aimed at elucidating the genetic basis of autistic disorder, especially it's relationship to fragile-X syndrome. The detection of fragile X chromosome(Xq27.3) by cytogenetic analysis has revealed many difficulties in testing. Therefore, to explore the relationship between autistic disorder and fragile X syndrome, this study administered molecular biologic methods which examined an unstable CGG repeat within the fragile X mental retardation-1(FMR-1) gene. Methods : Ninety nine autistic children and eight normal control children were tested. The number of CGG repeats within FMR-1 gene was measured after amplification by PCR, and cytogenetic analysis was also carried out to detect fragile site Xq27.3. Sourthern blot hybridization, using StB12.3 and/or Pfxa3 probe, was done for the patients showing expansion of more than 50 CGG repeats(premutation). Results : All but two autistic patients had no expansion in CGG repeats by PCR and there was no significant statistical difference in number of CGG repeat in comparison with normal control. Two autistic patients, considered as premutation by PCR analysis, had no full mutation or premutation by Southern blot hybridization. All autistic children tested did not have any abnormal karyotype or fragile site Xq27.3. Conclusions : These results suggest that autistic patients may not have abnormality in FMR-1 gene or abnormal expansion in CGG repeat. In conclusion, fragile X syndrome may not be antecedent of autistic disorder. KEY WORDS : Autistic disorder·FMR-1 gene trinucleotide repeats·Molecular biologic analysis.

全北地方의 藥用 資源植物 分布

郭晙洙,金昌洙,宋英恩,金柱,朴珍華,韓宗鉉,宋昊俊 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

국민 소득이 늘어나고 건강에 대한 관심이 높아지면서 한약재에 대한 수요가 증가하게 되고 WTO체제의 출범과 함께 선진각국의 유전자원 확보전이 치열해지면서 국내에서도 약용자원식물의 분포상황 및 이들 유전자원 식물의 보전이 중요한 과제로 대두되고 있다. 따라서 전북지방의 약초자원식물의 분포상황 및 이들 유전자원식물의 보전이 중요한 과제로 대두되고 있다. 따라서 전북지방의 약초자원식물의 분포현황을 조사하여 그에 대한 보전대책 수립 및 연구의 기초자료를 얻고자 수행한 본연구의 주요결과는 다음과 같다. 1. 전북지방에 분포하는 약용 자원식물의 목록을 작성한 결과 총 40목 129과에 걸쳐 762종류의 식물이 정리되었다. 2. 이들 분포식물중 항암효과가 보고된 약용식물은 36과 74종류였다. 3. 지역적으로는 덕유산지역에 가장 다양한 식물이 반포하고 다음으로는 남원, 변산반도, 모악산, 무주군, 완주고산, 내장산, 장안산, 선운산, 비안도, 적상산 등의 순으로 나타났다. 4. 식물군별로는 쌍자엽식물강의 이판화군과 합판화군이 가장 다양하였고, 각 목별 과(科) 수는 통화식물목이 10개 과로서 가장 많았으며, 각 목별 종류 수에 있어서는 장미목이 99종으로 가장 많았고, 초롱꽃목(77종), 통화식물목(67종), 백합목(53종), 미나리아재비목(43종), 산형화목(39종) 등이 비교적 많은 종들을 포함하는 것으로 분석되었다. 5. 각 과별 종수 분석에서는 국화과가 70종으로 가장 많았고, 장미과(46종), 콩과(40종), 백합과(40종)등으로 나타나 이들 4개과의 합계 종수가 196종류로서 전체의 25.72%를 차지하였다. 그 다음으로 산형과(27종), 미나리아재비과(26종), 꿀풀과(25종), 벼과(22종), 마디풀과(19종), 현삼과(15종), 석죽과(12종), 제비꽃과(11종), 난초과(11종) 등이 비교적 많은 종수를 가지는 과(科)들이었다. With the improving of the people's standard of life and the heightening of the concerns of health, the demands of medicinal plants are more rapidly increase. Also, with the inauguration of World Trade Organization, and acceleration of openness in domestic markets, the procurement competitions of gene pools in medicinal plants are more intense among the countries. With these trends of the world, an acute subjects are coming up for us such as distribution status quo and preservation of medicinal plants in domestic area. Therefore this paper surveyed the distribution status quo of medicinal plants in Chonbuk province, arranged the catalogue and analyzed the data by plants and regions. The major results are as follows; 1. The total amounts of 762 species, 129 families, 40 orders of medicinal plants distributed in Chonbuk province. 2. The number of medicinal plants which are assumed to contain antineoplastic agents totals 36 families and 74 species. 3. In distribution of medicinal plants by region, the most diverse flora showed in Mt. Dukyoo followed by circumferences of Namwon, Byun-san, Mt. Moak, Muju, Dosan, Mt. Naejang, Mt. Jangan, Mt. Seon-woon, Beeando island and Mt. Juksang etc. in Chonbuk province.

Azoloquinoxaline類의 간편한 合成에 관한 硏究 : 第1報

郭美玉,南順花,金恩慶,金眞姬,韓盛旭,李萬佶,朴義煥,金浩植 고신대학교 자연과학연구소 1993 고신대학교 자연과학연구소 논문집 Vol.3 No.-

The reaction of 2, 6-dichoroquinoxaline with m-chloroperbenzoic acid gave 2, 6-dichloroquinoxaline 4-oxide, whose reaction with sodium azide provided 7-chlorotetrazolo[1, 5-a]quinoxaline 5-oxide and not the isomeric azide. The structures of the synthesized compounds were confirmed on the basis of IR, ¹H-NMR, elemental analysis and mass spectral data.

부인과적 진단 복강경술에 대한 임상적 고찰

곽재환,박진완,황태영,김현호,이원기 동아대학교의과대학 1992 동아의대학술지 Vol.4 No.1

여자 500m 쇼트트랙 스피드 스케이팅의 스타트 기술분석

백진호,곽창수,정남주 한국운동역학회 2004 한국운동역학회지 Vol.14 No.3

J. H. BACK, C. S. KWAK, N. J. CHUNG. Analysis of the Female 500m Sprint Starting Motion in Short Track Speed Skating. Korean Journal of Sport Biomechanics, Vol. 14, No. 3, pp. 285-299, 2004. The purpose of this study is to identify female 500m sprint start motion by the center of gravity position in short track speed skating. The center of gravity position ratio was divided into three type(type A front : 80%-back : 20%, type B front : 70%-back : 30%, type C front: 50%-back : 50%). Three video cameras were used for 3D motion analysis with DLT method and the results were as follows: The elapsed time in starting motion was appeared that type B was the shortest and type A was the longest. It was appear that the stroke length of type A was longer than that type B and C during starting phase. This result was similar to displacement of center of gravity. It was appeared that skill type of center of gravity position ratio type B' ankle and knee joint angle were lower than that of type A and C. Observing these results it was conclusion that skill type B of center of gravity position ratio was more faster than that of type A and C. But it is important that these skill type needed to verifying more subjects.