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Isolation and Characterization of Novel Alginate-Degrading Pseudoalteromonas sp. Y-4
Cho, Hyeon-Ah,Kim, Hyun-Woo,Kim, Young-Mog The Korean Society of Fisheries and Aquatic Scienc 2012 Fisheries and Aquatic Sciences Vol.15 No.3
To isolate an alginate-degrading bacterium, we conducted a single colony isolation using a solid medium containing alginate as the sole carbon source. A marine bacterium Y-4 capable of degrading alginate was isolated from seawater. The strain was identified to be Pseudoalteromonas sp., based on morphological, biochemical, 16S rDNA homology, and phylogenetic analyses. Moreover, Pseudoalteromonas sp. Y-4 exhibited alginate lyase activity in the presence of 4% alginate even though many known alginate-degrading bacteria degrade in the range of 0.5-1% alginate. The optimum culture conditions for the Y-4 strain were 2% alginate, pH 8.0, and 3% NaCl at $30^{\circ}C$. The highest alginate lyase activity was also observed under the same conditions. To our knowledge, this is the first reported isolation of a marine bacterium degrading high concentrations of alginate.
Isolation and Characterization of Novel Alginate-Degrading Pseudoalteromonas sp. Y-4
( Hyeon Ah Cho ),( Hyun Woo Kim ),( Young Mog Kim ) 한국수산과학회(구 한국수산학회) 2012 Fisheries and Aquatic Sciences Vol.15 No.3
To isolate an alginate-degrading bacterium, we conducted a single colony isolation using a solid medium containing alginate as the sole carbon source. A marine bacterium Y-4 capable of degrading alginate was isolated from seawater. The strain was identified to be Pseudoaberomonas sp., based on morphological, biochemical, 1 6S rDNA homology, and phylogenetic analyses. Moreover, Pseudoalleromonas sp. Y-4 exhibited alginate lyase activity in the presence of 4% alginate even though many known alginatedegrading bacteria degrade in the range of 0.5-1% alginate. The optimum culture conditions for the Y-4 strain were 2% alginate, pH 8.0, and 3% NaCl at 30°C. The highest alginate lyase activity was also observed under the same conditions. To our knowledge, this is the first reported isolation of a marine bacterium degrading high concentrations of alginate.
단보 : Chinese Hamster Lung Cell의 소핵시험을 이용한 식물추출물 유기농업자재의 유전독성평가
조현조 ( Hyeon Jo Cho ),박경훈 ( Kyung Hun Park ),정미혜 ( Mi Hye Jeong ),박수진 ( Soo Jin Park ),오진아 ( Jin Ah Oh ),김원일 ( Won Il Kim ),조남준 ( Nam Jun Cho ),류재기 ( Jae Gee Ryu ),백민경 ( Min Kyoung Paik ) 한국환경농학회 2014 한국환경농학회지 Vol.33 No.2
님추출물과 고삼추출물은 식물추출물 유기농업자재로 우리나라에 등록되어 친환경농산물 재배에 널리 사용되고 있다. 본 연구에서는 님추출물 2종과 고삼추출물 2종에 대한 안전성을 확인하고자 Chinese hamster lung cells을 이용한 in vitro 소핵시험(vitMN)을 통해 유전독성을 평가하였다. 시험은 cytochalasin B 적용군과 비적용군으로 구분하여 시험하였으며, 양성대조물질로 mitomycin C와 colchicine을 사용하였다. 시험 결과, 님추출물 시료 2종과 고삼추출물 시료 2종은 처리 농도에 따라 소핵발생 비율이 증가하였으나 모든 시료 처리군에서 전체 발생률이 2.2% 미만으로, 음성대조군대비 2배 미만의 소핵이 관찰되었다. 따라서 님추출물 및 고삼추출물 시료는 본 시험의 vitMN 결과 최종적으로 음성으로 확인되었다. 또한 본 연구와 동일한 시료를 이용한 선행연구에서 복귀돌연변이 시험에서 음성을 보인 결과를 근거로 battery system에 의거해 종합적으로 평가하였을 때 님추출물과 고삼추출물 시료는 유전독성을 일으키지 않는 것으로 최종 판정하였다. BACKGROUND: Azadirachta Indica extract(AIE) and Sophorae radix extract(SRE) are widely used as environment-friendly organic materials of plant origin in South Korea. METHODS AND RESULTS: In this study, the in vitro micronucleus(vitMN) tests of two samples of AIE and SRE were conducted to evaluate their genotoxicity using the Chinese hamster lung(CHL) cell. This study was composed of two parts; cytochalasin B(cyto B) test and non-cyto B test. Mitomycin C and colchicine were used as positive controls. As a result, the incidence of micronucleus(MN) in all AIE and SRE treated groups increased in dose- dependent manner, but were less than 2.2% in 1,000 binucleated cells. In addition, there were no significant increases of MN incidence in all AIE and SRE treated groups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samples and SRE samples used in this study may have no genotoxicity in the in vitro micronucleus test using the CHL cells. In our previous study, we reported that AIE and SRE did not cause genotoxicity in Ames test. According to the genotoxicity battery system, we concluded that AIE and SRE used in this study have no genotoxic effects to humans.
국내 표준화 환자를 활용한 시뮬레이션 교육에 관한 주제범위 문헌고찰
조현아(Hyeon Ah Cho),박선정(Sun Jung Park) 한국간호연구학회 2022 한국간호연구학회지 Vol.6 No.3
Purpose : This study is a scoping literature review on simulation education using domestic standardized patients based on the scoping literature review protocol presented by The Joanna Briggs Institute (JBI), and provides basic data for future simulation education using standardized patients. Method : The literature search was conducted through 4 domestic sources (DBpia, KISS, KMBASE, NDSL), and documents published from January 2011 to December 2021. The main search terms were ‘standardized patient’ and ‘simulation’. As a result, 21 articles that met the selection criteria were selected. Results : The results show that quantitative research was the largest with 17 (81.0%) out of a total of 21 articles, and that nursing simulation education as the main topic (title) had the most articles with 7 (33.3%). Communication ability was found to be the most common variable with 8 pieces (38.1%). Conclusion : By means of the analysis results of the literature review on the subject range of simulation education using domestic standardized patients, it will be possible to use them as meaningful data to improve the effectiveness and quality of simulation education using standardized patients.
조현아(Hyeon Ah Cho),박선정(Sun Jung Park),우희영(Hee Yeong Woo),장영은(Young Eun Jang),이선우(Sunwoo Lee),배소현(So Hyun Bae) 한국간호연구학회 2021 한국간호연구학회지 Vol.5 No.2
Purpose : This study aims to provide basic data for future research direction on infection related to nursing students by analyzing and confirming the trends of infection-related research targeting domestic nursing students. Methods : Literature search was conducted through four databases (KERIS, Nanet, KoreaMed, KISS), and literatures published from 2011 to April 2021 were targeted. Key words were searched by combining ‘infection’, ‘nursing infection’, ‘infection education’, and ‘infection education for nursing college students’. 54 research papers that meet the selection criteria were selected for the final analysis. Results : As a result of the study, the most common studies related to the guidelines of standard attention were found to have significant effects on the knowledge, attitude and performance of infection, which are the main factors. Conclusion : This study is meaningful in developing infection-related research in nursing college students by closely analyzing and presenting the trends of infection-related research.
Cho Yuri,Choi Jin Woo,권훈,Kim Kun Yung,Lee Byung Chan,Chu Hee Ho,Lee Dong Hyeon,Lee Han Ah,Kim Gyoung Min,Oh Jung Suk,Hyun Dongho,Lee In Joon,Rhim Hyunchul 대한영상의학회 2023 Korean Journal of Radiology Vol.24 No.7
Transarterial chemoembolization (TACE) was introduced in 1977 with the administration of chemotherapeutic agent to gelatin sponge particles through the hepatic artery in patients with hepatocellular carcinoma (HCC) and was established as conventional TACE using Lipiodol in the 1980s. In the 2000s, drug-eluting beads were developed and applied clinically. Currently, TACE is a commonly used non-surgical treatment modality for patients with HCC who are unsuitable for curative treatment. Considering the vital role of TACE in the management of HCC, it is crucial to organize current knowledge and expert opinions regarding patient preparation, procedural techniques, and post-treatment care in TACE, which can enhance therapeutic efficacy and safety. A group of 12 experts in the fields of interventional radiology and hepatology, convened by the Research Committee of the Korean Liver Cancer Association (KLCA), has developed expert consensus-based practical recommendations in TACE. These recommendations have been endorsed by the Korean Society of Interventional Radiology and provide useful information and direction in performing TACE procedure as well as pre- and post- procedural patient care.