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Dong-Shan Fu,Yan-Liang Xu 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.5
In order to overcome the manufacturing difficulty of the transverse-flux permanent magnet linear motor (TFPMLM) and enhance its performance much better, a novel TFPMLM with Ecore and 3 dimension (3D) magnetic structures is proposed in this paper. Firstly, its basic structure and operating principle are presented. Then the equivalent 2D configuration of the TFPMLM is transformed, so that the Schwarz-Christoffel (SC) mapping method can be used to analyze the motor. Furthermore, the air gap flux density distribution is solved by SC mapping method, based on which, the EMF waveform, no-load cogging force waveform and load force waveform are obtained. Finally, the prototyped TLPMLM is manufactured and the results are obtained from the experiment and 3D FEM, respectively, which are used to compare with those from SC mapping method.
Analysis on Response Prediction of a Single Pile and Pile Groups Based on the Runge-Kutta Method
Dong-dong Pan,Qian-qing Zhang,Shan-wei Liu,Shi-min Zhang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.1
The conventional load-transfer approach can not consider the interaction of piles, and can not be directly used in the analysis of the response of pile groups. The interactive effects among piles can be taken into account in the shear displacement method based on the principle of superposition. In the present analysis, the shaft displacement of a single pile at a given depth is assumed to be composed of the pile-soil relative displacement developed at the disturbed soil around pile and the elastic vertical soil displacement developed in the soil mass. A new load-transfer function which is very similar to a hyperbolic model is then established to describe the relationship between unit skin friction and shaft displacement at a given depth. Furthermore, a hyperbolic model is used to simulate the relationship between unit end resistance and pile end displacement. As to the analysis of the response of pile groups, the interactive effects among piles are considered by using the shear displacement method. For an individual pile in pile groups, new load-transfer functions are established to capture the relationship between unit skin friction and shaft displacement and the relationship between end resistance and pile end displacement. Based on the fourth order Runge-Kutta method, a modified loadtransfer method is proposed to analyze the response of pile groups considering the interactive effects among piles. The reliability of the present method is checked by using comparisons of the present calculated results, the measured results and the computed values derived from other methods.
Gram-Scale Production of Ginsenoside F1 Using a Recombinant Bacterial β-Glucosidase
( Dong-shan An ),( Chang-hao Cui ),( Muhammad Zubair Siddiqi ),( Hong Shan Yu ),( Feng-xie Jin ),( Song-gun Kim ),( Wan-taek Im ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.9
Naturally occurring ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) is rare. Here, we produced gram-scale quantities of ginsenoside F1 from a crude protopanaxatriol saponin mixture comprised mainly of Re and Rg1 through enzyme-mediated biotransformation using recombinant β-glucosidase (BgpA) cloned from a soil bacterium, Terrabacter ginsenosidimutans Gsoil 3082<sup>T</sup>. In a systematic step-by-step process, the concentrations of substrate, enzyme, and NaCl were determined for maximal production of F1. At an optimized NaCl concentration of 200 mM, the protopanaxatriol saponin mixture (25 mg/ml) was incubated with recombinant BgpA (20 mg/ml) for 3 days in a 2.4 L reaction. Following octadecylsilyl silica gel column chromatography, 9.6 g of F1 was obtained from 60 g of substrate mixture at 95% purity, as assessed by chromatography. These results represent the first report of gram-scale F1 production via recombinant enzyme-mediated biotransformation.
Dong-Shan An,Muhammad Zubair Siddiqi,김경호,Hong Shan Yu,임완택 한국미생물학회 2018 The journal of microbiology Vol.56 No.1
A taxonomic study was conducted on BR7-21T, a bacterial strain isolated from the soil of a ginseng field in Baekdu Mountain. Comparative studies of the 16S rRNA gene sequence showed that the isolate was most closely related to Conexibacter woesei DSM 14684T, Solirubrobacter pauli ATCC BAA-492T, Patulibacter minatonensis JCM 12834T, with 93.8%, 92.4%, and 91.5% sequence similarity, respectively; each genus represented a family in the order Solirubrobacterales. Strain BR7-21T was Gram-reaction positive, non-spore forming, aerobic, non-motile, and short rod-shaped. It grew well on half-strength R2A medium. The G + C content of the genomic DNA was 73.9%. It contained meso-diaminopimelic acid in the cell wall and the major menaquinones were MK-7(H4) and MK-8(H4). The major fatty acids were summarized as (C16:1 ω7c/iso-C15:0 2-OH), iso-C16:0, and C17:0 cyclo. On the basis of polyphasic evidence, it was proposed that strain BR7- 21T should be placed in a new genus and species, for which the name Baekduia soli gen. nov., sp. nov. was proposed with the type strain BR7-21T (= KCTC 22257T = LMG 24797T). The family Baekduiaceae fam. nov. is proposed to encompass the genus Baekduia gen. nov.
Shan, Ying,Dong, Ying,Jiang, Dong 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6
Antibiotic peptides are a battery of broad-spectrum antibacterial cationic polypeptides widely distributed in the plant and animal kingdoms. Among them, the human defensins are the first line of defense against pathogens and Mytilin, which is isolated from mussel serum, plays a key role in the mussel defense system. The antibacterial activity of these two peptides is generally ascribed to their overall positive charge, which enables them to disrupt bacterial membrane integrity and function. The aim of this study was to develop an effective method for the biosynthesis of a fusion peptide containing human a-defensin 5 (HD5) and Mytilin-1 in Escherichia coli to improve the antimicrobial activity. The individual HD5 and Mytilin-1 peptides were also synthesized for comparison with the fusion peptide. All the peptides, expressed as soluble fusions with the peptide thioredoxin, were isolated using a three-step purification strategy involving nickel-Sepharose chromatography, enterokinase cleavage, and cationic exchange chromatography. The identity of the peptides was confirmed by SDS-PAGE. Antimicrobial activity assays demonstrated that all the recombinant peptides had strong bactericidal properties and that the HD5 and Mytilin-1 fusion protein displayed higher activity against E. coli and Staphylococcus aureus. The results of this study provide a platform for the development of novel cationic peptides for biological studies.
Cough Inhibition Activity of Schisandra chinensis in Guinea Pigs
Shan Zhong,Li-Ping Bai,Xiao-Dong Liu,Die-Yi Cai,Lee-Fong Yau,Chu-Qin Huang,Jia-Qi Zhang,Ke-Fang Lai,Nan-Shan Zhong 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.4
Chronic cough is very common in respiratory clinics, and no effective drugs are available. Schisandra chinensis (Turcz.) Baill. (S. chinensis), an important traditional Chinese medicine, has been extensively prescribed for patients with a persistent cough. Preliminary research indicated that 95% ethanol extracts (EE) of S. chinensis showed remarkable antitussive activity in guinea pigs exposed to cigarette smoke (CS). To find out the antitussive ingredients of S. chinensis, EE was divided into four fractions according to the polarity: petroleum ether extract (PEE), ethyl acetate extract (ECE), n-butyl alcohol extract, and residue extract. The antitussive, antioxidant, and anti-inflammatory effects of the four fractions were evaluated in a guinea pig model of cough hypersensitivity induced by CS exposure. Eighteen main constituents of the two effective fractions, PEE and ECE, were identified using ultra-high-pressure liquid chromatography electronic spray ion time-of-flight mass spectrometry. The cough inhibition activities of compound 1, 3, 9, 10, 17 were evaluated on citric acid induced acute cough guinea pigs. The results showed that the antitussive activity of EE was almost all contained in PEE and ECE. The 16 major peaks in PEE were identified as 15 lignans (1–12 and 14–16) and 1 triterpene (compound 13), and 3 major peaks (1, 17, and 18) in ECE were also identified as lignans. Three doses of five compounds brought about a significant decrease in number of cough efforts (P < .01), and the cough inhibition rates were between 40.9% and 85.1%. Therefore, lignans are the antitussive ingredients of S. chinensis.
The treatment effect of novel hGHRH homodimer to male infertility hamster
Xu-Dong Zhang,Xiao-Yuan Guo,Jing-Xuan Tang,Lin-Na Yue,Juan-Hui Zhang,Tao Liu,Yu-Xia Dong,Song-Shan Tang 대한생리학회-대한약리학회 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.6
Extra-hypothalamic growth hormone-releasing hormone (GHRH) plays an important role in reproduction. To study the treatment effect of Grin (a novel hGHRH homodimer), the infertility models of 85 male Chinese hamsters were established by intraperitoneally injecting 20 mg/kg of cyclophosphamide once in a week for 5 weeks and the treatment with Grin or human menopausal gonadotropin (hMG) as positive control was evaluated by performing a 3-week mating experiment. 2-8 mg/kg of Grin and 200 U/kg of hMG showed similar effect and different pathological characteristics. Compared to the single cyclophosphamide group (0%), the pregnancy rates (H-, M-, L-Grin 26.7, 30.8, 31.3%, and hMG 31.3%) showed significant difference, but there was no difference between the hMG and Grin groups. The single cyclophosphamide group presented loose tubules with pathologic vacuoles and significant TUNEL positive cells. Grin induced less weight of body or testis, compactly aligned tubules with little intra-lumens, whereas hMG caused more weight of body or testis, enlarging tubules with annular clearance. Grin presented a dose-dependent manner or cell differentiation-dependentincrease in testicular GHRH receptor, and did not impact the levels of blood and testicular GH, testosterone. Grin promotes fertility by proliferating and differentiating primitive cells through up-regulating testicular GHRH receptor without triggering GH secretion, which might solve the etiology of oligoasthenozoospermia.
Fu, Dong-Shan,Xu, Yan-Liang The Korean Institute of Electrical Engineers 2017 Journal of Electrical Engineering & Technology Vol.12 No.5
In order to overcome the manufacturing difficulty of the transverse-flux permanent magnet linear motor (TFPMLM) and enhance its performance much better, a novel TFPMLM with E-core and 3 dimension (3D) magnetic structures is proposed in this paper. Firstly, its basic structure and operating principle are presented. Then the equivalent 2D configuration of the TFPMLM is transformed, so that the Schwarz-Christoffel (SC) mapping method can be used to analyze the motor. Furthermore, the air gap flux density distribution is solved by SC mapping method, based on which, the EMF waveform, no-load cogging force waveform and load force waveform are obtained. Finally, the prototyped TLPMLM is manufactured and the results are obtained from the experiment and 3D FEM, respectively, which are used to compare with those from SC mapping method.
An, Dong-Shan,Im, Wan-Taek,Yang, Hee-Chan,Lee, Sung-Taik Society for General Microbiology 2006 International journal of systematic and evolutiona Vol.56 No.2
<P>The taxonomic position of a novel bacterial strain, Ch06T, isolated from an upflow anaerobic sludge blanket reactor was determined. Strain Ch06T was Gram-negative, aerobic, motile and oxidase- and catalase-positive. A comparative 16S rRNA gene sequence analysis showed a clear affiliation of strain Ch06T to the Alphaproteobacteria and it was most closely related to Zoogloea ramigera ATCC 19623 and Mycoplana dimorpha IAM 13154T (97.9 and 96.3% sequence similarity, respectively). The major respiratory quinone was Q-10 and the predominant fatty acids were C16:0, 3-OH C16:0, C18:0, C19:0 cyclo omega8c and summed feature 7 (C18:1omega7c/omega9t/omega12t, C18:1omega7c/omega9c/omega12t). On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the novel isolate was assigned to a new genus, Shinella gen. nov., as Shinella granuli gen. nov., sp. nov. (type strain Ch06T=KCTC 12237T=JCM 13254T). It is proposed that Zoogloea ramigera ATCC 19623 is reclassified into the novel genus Shinella as Shinella zoogloeoides sp. nov. (type strain ATCC 19623T=IAM 12669T=I-16-MT).</P>