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Enhanced shoot and bulblet proliferation of Garlic (Allium sativum L.) in bioreactor system
Kim, E. K.,Hahn, H. N.,Murthy, H. N.,Paek, K. Y 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-
Two types of Ballon Type Bubble Bioreactors (BTBB, air lift type), continuous immersion cultures (with and without raft) and temporary immersion cultures (ebb and flood) were used for mass multiplication of shoots and bulblets of Garlic cv. Danyang. Murashige and Skoog liquid medium supplemented with 2% sucrose was used for shoot proliferation and shoot proliferation was achieved in three weeks. MS liquid medium supplemented with 11% sucrose and 0.1 mgl-1 naphthalene acetic acid was used for bulblet induction and growth and bulblet growth was achieved in nine weeks in bioreactor cultures. The continuous immersion type of bioreactors was found suitable for shoot proliferation and twenty-seven shoots were developed from each explant. Biomass of shoots was also highest in this method. Inoculation density of fifteen shoots per culture was suitable and aeration of bioreactors with sparger (0.1 air volume/medium volume/min) influenced the biomass production. Immersion type (with raft) bioreactor was suitable for bulblet formation, The ratio of innoculum size and media volume affected the growth values. Cold treatment of cultures at 40C for three weeks enhanced the formation of bulbelts. The bulblets were harvested at the end of culture period and were given cold treatment 40C for eight weeks, transferred to a potting mixture of peat moss and perlite (1:1). The bulblets, which were more than 0.2 g, sprouted (90%) in two weeks of trnsplantation.
Murthy, Indu K.,Bhat, Savithri,Sathyanarayan, Vani,Patgar, Sridhar,M., Beerappa,Bhat, P.R.,Bhat, D.M.,Gopalakrishnan, Ranjith,Jayaraman, Mathangi,Munsi, Madhushree,N.H., Ravindranath,M.A., Khalid,M., Institute of Forest Science 2014 Journal of Forest Science Vol.30 No.2
The tropical wet evergreen, tropical semi evergreen and moist deciduous forest types are projected to be impacted by climate change. In the Western Ghats region, a biodiversity hotspot, evergreen forests including semi evergreen account for 30% of the forest area and according to climate change impact model projections, nearly a third of these forest types are likely to undergo vegetation type change. Similarly, tropical moist deciduous forests which account for about 28% of the forest area are likely to experience change in about 20% of the area. Thus climate change could adversely impact forest biodiversity and product flow to the forest dependent households and communities in Uttara Kannada district of the Western Ghats. This study analyses the distribution of non-timber forest product yielding tree species through a network of twelve 1-ha permanent plots established in the district. Further, the extent of dependence of communities on forests is ascertained through questionnaire surveys. On an average 21% and 28% of the tree species in evergreen and deciduous forest types, respectively are, non-timber forest product yielding tree species, indicating potential high levels of supply of products to communities. Community dependence on non-timber forest products is significant, and it contributes to Rs. 1199 and Rs. 3561/household in the evergreen and deciduous zones, respectively. Given that the bulk of the forest grids in Uttara Kannada district are projected to undergo change, bulk of the species which provide multiple forest products are projected to experience die back and even mortality. Incorporation of climate change projections and impacts in forest planning and management is necessary to enable forest ecosystems to enhance resilience.
Cui, H.Y.,Murthy, H.N.,Moh, S.H.,Cui, Y.Y.,Lee, E.J.,Paek, K.Y. Elsevier 2014 Industrial crops and products Vol.53 No.-
Protocorm suspension cultures of Dendrobium candidum were established in balloon type bubble bioreactors for the production of biomass and bioactive compounds. Various culture parameters namely culture method, inoculum density and aeration volume were optimized. Immersion cultures found to be superior as compared to raft and ebb and flood methods in terms of accumulation of optimum biomass and bioactive compounds. An inoculums density of 50gl<SUP>-1</SUP> was found suitable for accumulation of biomass and bioactive compounds. An aeration volume of 0.1vvm was found suitable for the accumulation of biomass, whereas aeration volume of 0.3vvm was found suitable for production of polysaccharides, coumarins, polyphenolics, flavonoids, vitamin C and vitamin E. The results of the current study are useful for scale-up process for the production of bioactive compounds from D. candidum protocorm suspension cultures.
Thanh, N.T.,Murthy, H.N.,Paek, K.Y. Elsevier 2014 INDUSTRIAL CROPS AND PRODUCTS Vol.60 No.-
Ginseng cell suspension culture was established in 5l capacity balloon type airlift bioreactors using Murashige and Skoog medium supplemented with 7mgl<SUP>-1</SUP> indole butyric acid, 0.5mgl<SUP>-1</SUP> kinetin and 30gl<SUP>-1</SUP> sucrose. Various parameters such as types of bioreactors (cylinder, balloon, bulb and cone type bioreactors), aeration volume (0.05, 0.1, 0.2, 0.3vvm constant air supply or the amount air supply was increased from 0.05 to 0.3vvm at 6-day intervals), and inoculum density (40, 60, 80 and 100gl<SUP>-1</SUP>) were optimized. Balloon type airlift bioreactors, aeration volume of 0.1vvm, and inoculum density of 60gl<SUP>-1</SUP> were found suitable for accumulation of ginseng cell biomass and ginsenosides. Based on optimized results, 500l capacity large-scale drum type and balloon type bioreactors were established for the production of ginsenosides. Optimal yield of 187kg and 400kg fresh biomass and 6.2kg and 13.3kg dry biomass, 7.86mgg<SUP>-1</SUP> DW and 7.75 mgg<SUP>-1</SUP> DW total ginsenosides could be achieved in 500l capacity drum and balloon type bioreactors respectively. These results are useful for commercial production of ginseng cell biomass and secondary metabolites.