Oral Session : Comparison of Physical Fitness between Chinese and Korean Collegians

( Zong Ping Wang ),( Cheng Jun Cui ),( Byoung Goo Ko ) 한국체육학회 2013 In Pursuit of Evidence-Based Sport and Exercise Sc Vol.0 No.-

Asymmetric Reduction of Ethyl Acetoacetate Catalyzed by Immobilized Acetobacter sp. CCTCC M209061 Cells in Hydrophilic Ionic Liquid Hybrid System

Ping Wei,Pei Xu,Xiao-Ting Wang,Wen-Yong Lou,Min-Hua Zong 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.2

The asymmetric reduction of ethyl acetoacetate (EAA) to ethyl (R)-3-hydroxybutyrate [(R)-EHB] using immobilized Acetobacter sp. CCTCC M209061 cells was successfully conducted in a hydrophilic ionic liquid (IL)- containing system. The best one of all the tested watermiscible ILs was 1-(hydroxyethyl)-3-methylimidazolium hydrochloride (C2OHMIM·Cl). In C2OHMIM·Cl-aqueous buffer hybrid system, it was found that the optimal IL concentration, substrate and co-substrate concentration, reaction temperature, buffer pH and shaking rate were 0.5mol/L, 45 mmol/L, 80 mmol/L, 35oC, pH 5.5 and 200 rpm, respectively. Under the optimized reaction conditions, the initial reaction rate, the yield and the product e.e. reached 4.90 μmol/min, 95.3 and > 99.0%, respectively, which were much higher than the corresponding values reported previously. The efficient biocatalytic process mediated by the immobilized cells was feasible on 500 mL preparative scale, and the biocatalysts showed good operational stability and could be recycled for at least 10 batches.

ZNF488 Enhances the Invasion and Tumorigenesis in Nasopharyngeal Carcinoma Via the Wnt Signaling Pathway Involving Epithelial Mesenchymal Transition

Dan Zong,Li Yin,Qian Zhong,Wen-jie Guo,Jian-hua Xu,Ning Jiang,Zhi-rui Lin,Man-zhi Li,Ping Han,Lin Xu,Xia He,Mu-sheng Zeng 대한암학회 2016 Cancer Research and Treatment Vol.48 No.1

Purpose The purpose of this study was to investigate the function of Zinc finger protein 488 (ZNF488) in nasopharyngeal carcinoma (NPC). Materials and Methods The endogenous expression of ZNF488 in NPC tissues, normal nasopharyngeal epithelium tissues and NPC cell lines were detected by quantitative reverse transcription polymerase chain reaction. ZNF488 over-expressing and knock-down NPC cell line models were estab- lished through retroviral vector pMSCV mediated over-expression and small interfering RNA (siRNA) mediated knock-down. The invasion and migration capacities were evaluated by wound healing and transwell invasion assays in ZNF488 over-expressing and control cell lines. Soft-agar colony formation and a xenograft experiment were performed to study tumorigenic ability in vitro and in vivo. Immunofluorescence and western blotting analysis were used to examine protein changes followed by ZNF488 over-expression. Microarray analysis was performed to explore gene expression profilings, while luciferase reporter assay to evaluate the transcriptive activity of Tcf/Lef. Results ZNF488 was over-expressed in NPC tissues compared with normal tissues, especially higher in 5-8F and S18, which are well-established high metastatic NPC clones. Functional studies indicate that over-expression of ZNF488 provokes invasion, whereas knock-down of ZNF488 alleviates invasive capability. Moreover, over-expression of ZNF488 promotes NPC tumor growth both in vitro and in vivo. Our data further show that over-expression of ZNF488 induces epithelial mesenchymal transition (EMT) by activating the WNT/β -catenin signaling pathway. Conclusion Our data strongly suggest that ZNF488 acts as an oncogene, promoting invasion and tumorigenesis by activating the Wnt/β -catenin pathway to induce EMT in NPC.

DO BURSAL AND ARTICULAR SIDE OF THE SUPRASPINATUS TENOON HAVE SAME COMPRESSIVE COMPLIANCE?

이석범,Zong-Ping Luo,Larry J. Berglund,Yi-Wyen Chang,Kai-Nan An 대한견주관절의학회 2000 대한견주관절학회 학술대회논문집 Vol.2000 No.5

Is Folate Status a Risk Factor for Asthma or Other Allergic Diseases?

Ting Wang,Zong-An Liang,Yu-Lin Ji,Hong-Ping Zhang,Xin Zhang,Gang Wang 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.6

Purpose: It is controversial whether folate status is a risk factor for the development of asthma or other allergic diseases. This study was conducted to investigate whether indirect or direct exposure to folate and impaired folate metabolism, reflected as methylene-tetrahydrofolate reductase (MTHFR) C677T polymorphism, would contribute to the development of asthma and other allergic diseases. Methods: Electronic databases were searched to identify all studies assessing the association between folate status and asthma or other allergic diseases. Two reviewers independently assessed the eligibility of studies and extracted data. The relative risk (RR) or odds ratio (OR) with 95% confidence intervals (CI) was calculated and pooled. Results: Twenty-six studies (16 cohort, 7 case-control, and 3 cross-sectional studies) were identified. Maternal folic acid supplementation was not associated with the development of asthma, atopic dermatitis (AD), eczema, and sensitization in the offspring, whereas exposure during early pregnancy was related to wheeze occurrence in the offspring (RR=1.06, 95% CI=[1.02-1.09]). The TT genotype of MTHFR C677T polymorphism was at high risk of asthma (OR=1.41, 95% CI=[1.07-1.86]). Conclusions: It is indicated that maternal folic acid supplementation during early pregnancy may increase the risk of wheeze in early childhood and that the TT genotype of MTHFR C677T polymorphism impairing folic acid metabolism would be at high risk of asthma development. These results might provide additional information for recommendations regarding forced folate consumption or folic acid supplements during pregnancy based on its well-established benefits for the prevention of congenital malformations. However, currently available evidence is of low quality which is needed to further elucidate.

Nonlinear vibration analysis of an embedded multi-walled carbon nanotube

Wu, Chih-Ping,Chen, Yan-Hong,Hong, Zong-Li,Lin, Chia-Hao Techno-Press 2018 Advances in nano research Vol.6 No.2

Based on the Reissner mixed variational theorem (RMVT), the authors present a nonlocal Timoshenko beam theory (TBT) for the nonlinear free vibration analysis of multi-walled carbon nanotubes (MWCNT) embedded in an elastic medium. In this formulation, four different edge conditions of the embedded MWCNT are considered, two different models with regard to the van der Waals interaction between each pair of walls constituting the MWCNT are considered, and the interaction between the MWCNT and its surrounding medium is simulated using the Pasternak-type foundation. The motion equations of an individual wall and the associated boundary conditions are derived using Hamilton's principle, in which the von $K{\acute{a}}rm{\acute{a}}n$ geometrical nonlinearity is considered. Eringen's nonlocal elasticity theory is used to account for the effects of the small length scale. Variations of the lowest frequency parameters with the maximum modal deflection of the embedded MWCNT are obtained using the differential quadrature method in conjunction with a direct iterative approach.

An IBR Approach Schema of Image Synthesis for Virtual Environment Rendering

Guo Ming-en,Ou Zong-ying,Hu Zhi-ping,Zhang Jing (사)한국CDE학회 2005 한국CAD/CAM학회 국제학술발표 논문집 Vol.2005 No.8

Study on the Gas-liquid Flow Characteristics in the Reversing Bucket of Water Jet Propulsion

Zhongyong Pan,Chunhai Cen,Ping Zong,Fall Ibra,Bo Yang 한국유체기계학회 2020 International journal of fluid machinery and syste Vol.13 No.2

To investigate the gas-liquid two-phase flow characteristics in the reversing bucket of water jet propulsion, the numerical simulations based on the Euler-Euler inhomogeneous model was conducted to the internal flow field of the reversing bucket under 1%~12% different inlet gas volume fractions (α). Based on that, the gas distribution, gas-liquid velocity streamlines and thrust changing were obtained to discuss the gas-liquid flow characteristics and the influence on the thrust of the reversing bucket. The calculation results show that the gas mainly distributes in the inner part of the reversing bucket flow passage under different inlet gas volume fractions. On the condition that the inlet gas volume fraction is more than 2%, the gas accumulation has occurred in the inlet section of the flow passage. With the increasing of inlet gas volume fraction, average velocity and pressure increase gradually at each cross-section of reversing bucket flow passage, and at the higher gas concentration in each cross-section, where the vortex will exist, indicating that the gas concentration has a great relationship with the vortex aggregation in the reversing bucket flow passage. When the inlet gas volume fraction is 7%, the thrust reaches the maximum. It shows that the appropriate gas can effectively improve the thrust efficiency of the reversing bucket.

N-acetylcysteine protects against cadmium-induced oxidative stress in rat hepatocytes

Jicang Wang,Huali Zhu,Xue-Zhong Liu,Zong-Ping Liu 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4

Cadmium (Cd) is a well-known hepatotoxic environmentalpollutant. We used rat hepatocytes as a model to studyoxidative damage induced by Cd, effects on the antioxidantsystems, and the role of N-acetylcysteine (NAC) in protectingcells against Cd toxicity. Hepatocytes were incubated for 12and 24 h with Cd (2.5, 5, 10 μM). Results showed that Cd caninduce cytotoxicity: 10 μM resulted in 36.2% mortality after12 h and 47.8% after 24 h. Lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase activitiesincreased. Additionally, reactive oxygen species (ROS)generation increased in Cd-treated hepatocytes along withmalondialdehyde levels. Glutathione concentrationssignificantly decreased after treatment with Cd for 12 h butincreased after 24 h of Cd exposure. In contrast, glutathioneperoxidase activity significantly increased after treatmentwith Cd for 12 h but decreased after 24 h. superoxidedismutase and catalase activities increased at 12 h and 24 h. glutathione S-transferase and glutathione reductase activitiesdecreased, but not significantly. Rat hepatocytes incubatedwith NAC and Cd simultaneously had significantly increasedviability and decreased Cd-induced ROS generation. Ourresults suggested that Cd induces ROS generation that leads to oxidative stress. Moreover, NAC protects rat hepatocytes from cytotoxicity associated with Cd.

Characterization of the Methylation Status of Pax7 and Myogenic Regulator Factors in Cell Myogenic Differentiation

Chao, Zhe,Zheng, Xin-Li,Sun, Rui-Ping,Liu, Hai-Long,Huang, Li-Li,Cao, Zong-Xi,Deng, Chang-Yan,Wang, Feng Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.7

Epigenetic processes in the development of skeletal muscle have been appreciated for over a decade. DNA methylation is a major epigenetic modification important for regulating gene expression and suppressing spurious transcription. Up to now, the importance of epigenetic marks in the regulation of Pax7 and myogenic regulatory factors (MRFs) expression is far less explored. In the present study, semi-quantitative the real-time polymerase chain reaction (RT-PCR) analyses showed MyoD and Myf5 were expressed in activated and quiescent C2C12 cells. MyoG was expressed in a later stage of myogenesis. Pax7 was weakly expressed in differentiated C2C12 cells. To further understand the regulation of expression of these genes, the DNA methylation status of Pax7, MyoD, and Myf5 was determined by bisulfite sequencing PCR. During the C2C12 myoblasts fusion process, the changes of promoter and exon 1 methylation of Pax7, MyoD, and Myf5 genes were observed. In addition, an inverse relationship of low methylation and high expression was found. These results suggest that DNA methylation may be an important mechanism regulating Pax7 and MRFs transcription in cell myogenic differentiation.