Oil Contents and Fatty Acid Composition of Korean Perilla (Perilla ocimoides L.) Collections

Hong,Seong-Taek,Son,Suk-Yeong,Jong,Seung-keun,Rho,Chang-Woo,Yun,Jong-Sun 한국자원식물학회 2003 Plant Resources Vol.6 No.3

This study was carried out to obtain informations on the variations of oil content and fatty acid composition among 90 Korean perilla collections. Average oil content of 90 perilla collections was 44.2% with a range from 29.7% to 61.9%. Perilla collections with late-maturing, super-large seed and gray seed coat showed higher oil content than other types in general. Average saturated fatty acid content in perilla oil was 9.0% with a range from 8.2% to 10.7%, while average unsaturated fatty acid content varied from 89.3% to 91.8% with a mean of 91.0%. Contents of saturated and unsaturated fatty acids were not related to maturity. There were no differences in the contents of saturated and unsaturated fatty acids according to maturity. Linoleic acid and linolenic acid contents were the highest in the super large-sized group(15.5%) and in the large-sized seed group(61.4%), respectively, while contents of fatty acids among the perilla collections were variable with different seed coat colors. Most of the traits studied were not significantly correlated with oil content, but linoleic acid($\omega$ 수식 이미지-6) content was negatively correlated (r=-0.217*) with linolenic acid(ω-3) content.

인체 대장암 및 자궁경부암에서 PCR-SSCP법을 이용한 Ki-ras 암유전자의 점돌연변이에 관한 연구

박영홍,백낙환,김현찬,김상효,홍관희,김기태,이기영 인제대학교 1994 仁濟醫學 Vol.15 No.2

인체 대장암 20례와 자궁경부암 10례의 암조직을 대상으로 c-Ki-ras 유전자 codon 12와 13에서의 점돌연변이를 알아보고자 polymerase chain reaction(PCR)과 single-strand conformation polymorphism(SSCP) 검사법을 시행하였다. 대장암의 경우 20례중 9례(45%)에서, 자궁경부암의 경우 10례중 1례(10%)에서 양성으로 나왔다. 대장암에서는 codon 12에서 GGT가 TGT로의 치환이 4례로서 가장 많았으며, AGT, CGT로의 치환이 각 1례였고 codon 13에서는 GGC가 GAC로의 치환이 2례, TGC로의 치환이 1례인 것으로 나타났다. 자궁경부암의 경우 codon 12에서 GGT가 AGT로의 치환이 1례인 것으로 나타났다. In an attempt to clarify the role of genetic alteration in the genesis of human colorectal and cervical cancers, tissue specimens from 20 patients with colorectal cancer and 10 patients with cervical cancer were examined for the presence of point mutation in K-ras2 exon 1 by single strand conformation polymorphism analysis of PCR product. Exon I of c-Ki-ras2 was amplified by polymerase chain reaction(PCR) and comparison was made between the normal and mutated genes by nondenaturing polyacrylamide gel electrophoresis(PAGE) of PCR product and nucleotide sequence analysis using asymmetric PCR with direct sequencing. Genomic DNA from white blood cells were used as normal control and those from A427 cell line were used as mutated control. 1.PCR product from A427 cell line showed a distinct migration shift pattern compared to the normal control in PAGE and the direct sequencing indicated that nucleotide sequence of codon 12 was mutated from GGT to GAT. In addition, there was a loss of normal allele in A427 cell line. 2.PCR product from 9 cases(45%) out of a 20 colon cancer patients showed migration shifts in PAGE, and all of these 9 patients invariably demonstrated mutation of ras gene, either in codon 12(6 cases:30%) or 13(3 cases:15%). The base substitutions in codon 12 were: from GGT to AGT(1), CGT(1), or TGT(4). Base changes in codon 13 were from GGC to TGC(1) or GAC(2). 3.PCR product from 1 cases(10%) out of a 10 cervical cancer patients showed migration shrifts in PAGE, Invariably demonstrated mutation of ras gene in codon 12. The base substitutions was from GGT to AGT(1) TQE incidence of point mutation of c-Ki-ras in colon cancer was high, however, it was low in cervical cancer, which showed the relation of ruts gene mutation with colon cancer. PCR -SSCP analysis is a simple, rapid and efficient method of detection of point mutation, especially when dealing with multiple samples.

超微量 銅의 螢光光度定量法

洪永大 고신대학교 자연과학연구소 1992 고신대학교 자연과학연구소 논문집 Vol.2 No.-

A spectrofluorometric method for the ultra microdetermination of copper was established. An excess amount of 2-(o-hydroxypheny1)benzoxazole(HPB) is added to precipitate cu(HPB)₂ with copper ion. Copper ion forms a chelate with HPB and quenches the fluorescence of HPB. The amount of copper is determined by measuring the fluorescence of the remaining HPB. The optimum conditions for these reactions were examined at a very low concentration range of the reactants. By the proposed method, 0.025~5 nmol copper content was determined with a accuracy of 2.1_(7)% relative standard deviation at 2 nmol Cu level.

대학 종합 관리 정보 시스템 개발에 관한 사례 연구 : 진주전문대학 입시관리시스템을 중심으로 Entrance Examination Management System of Chinju College

홍영표 진주여자전문대학 1995 論文集 Vol.18 No.-

The primary purpose of this study is to present CTMIS(College Total Management Information System) project for Enterance Examination that makes use of structured development techniques, in sufficient detail to use in a real project. The results of this study are summerized as follows: 1. Anaysis and design of College Total Management Information System. 2. Anaysis and design of the Entrance Examination System. 3. Apply of the structured analysis and design methodologies. 4. Development of the Entrance Examination System.

수면 부유 방법에 의한 연초 육묘

洪淳達,石泳善,朴孝澤 충북대학교 엽연초연구소 1999 煙草硏究 Vol.17 No.-

Carbonized rice hull, perlite, and vermiculite were mixed and investigated for the probability of tobacco transplant production by means of plug and transfer float system. These seedling bed materials in condition of float system have gaseous phase ranged from 6% to 13% showing a proper growth of tobacco seedling. However compost media mixed with soil, sand, and compost in condition of float system led to the shortage of respiration in root system by very low proportion of gaseous phase skewing a wicked growth of seedling. Absorption of heat by floating water for the daytime increased rhizosphere temperature in condition of float system during the night and low temperature season resulting in better growth of tobacco seedling. Consequently transplant production of tobacco seedling by float system with mixed materials of carbonized rice hull, perlite, and vermiculite was thought to be desirable method for manufacturing production system of transplant considering the effective management of water, nutrients, and temperature of seedling bed materials.

웹기반 저작도구 시스템 설계 및 구현

홍영표 진주여자전문대학 2000 論文集 Vol.23 No.-

To contract cycle education on the web, the instructor should be able to use web-related tools. And the contents so far have fixed screen composition and menu, which made the learner bored at class because they have to use same screen composition in each subject. This study makes the instructor conduct cycle education even if he can not use web tools. The instructor can compose various contents easily only by choosing an clicking menu for his class. He can also add functions by connecting what he whats to URL. For the learners, m will be composed of menu required only for the chosen class and screen composition will be different for each subject. This learners will have better studying environment because they have different screen composition for each subjects.

생물계면활성제를 이용한 중금속 및 유기물 오염 토양의 정화

홍석정,현재혁,김영철 한국공업화학회 2002 응용화학 Vol.6 No.1

A study on the soil remediation was performed by using a biosurfactant. The surfactant solutions were applied to the soil flushing of the artificially contaminated soil with copper ion, cadmium ion and phenol. The optimal concentration of the surfactant was determined by the lab.-scale column tests using the solutions of various concentrations. The characteristic behavior of the contaminant removal was studied and the efficiencies of the soil flushing were determined for the contaminants.

超微量 亞鉛의 螢光光度定量法

洪永大,李正禹 고신대학교 자연과학연구소 1997 고신대학교 자연과학연구소 논문집 Vol.7 No.-

超微量의 Zn을 定量하기 위하여 2-(o-hydroxyphenyl)benzoxazole (HPB)을 利用하는 高感度의 螢光分析法이 確立되었다. 0.65㎍ 以下의 Zn(Ⅱ) 이온을 含有하는 試料溶液에 이것에 比하여 一定過量의 HPB 溶液을 加하여 約 pH 9.4에서 反應시키면 螢光이 없고 安定度가 큰 Zn(HPB)_2 의 킬레이트 化合物을 만들어 定量的으로 沈澱한다. 그런 다음, 溶液 中에 남아 있는 HPB의 螢光强度를 測定함으로써 Zn(Ⅱ) 이온의 濃度를 間接 測定하였다. 本 硏究에서는 nmol level의 超微量 濃度에서 Zn(Ⅱ) 이온과 HPB의 反應이 化學量論的으로 일어 나는데 必要한 最適條件들을 綿密히 檢討한 結果, 0.5-10 nmol의 Zn을 感度좋게 定量할 수 있었다. 本 定量法을 使用하여 5 nmol의 Zn을 反復 測定하여 본 結果, 相對標準偏差 2.1%의 精密度로서 測定할 수 있었다. A spectrofluorometric method for the ultra microdetermination of zinc was established. An excess amount of 2- (o-hydroxt'phenyl)benzoxazole(HPB) is added to precipitate Zn(HPB)₂ with zinc ion. Zinc ion forms a chelate with HPB and quenches the fluorescence of HPB. The amount of zinc is determined by measuring the fluorescence of the remaining HPB. The optimum conditions for these reactions were examined at a very low concentration range of the reactants. By the proposed method, 0.5-10 nmol zinc content was determined with a accuracy of 2.1% relative standard deviation at 5 nmol Zn level.

重크롬酸칼륨과 鹽化바륨의 混酸溶液에 의한 微量 黃酸이온의 吸光光度定量

洪永大 고신대학교 자연과학연구소 1991 고신대학교 자연과학연구소 논문집 Vol.1 No.-

徵量의 黃酸이온을 보다 迅速正確하고 簡便하게 測定할 수 있는 定量法이 重크롬酸 칼륨과 鹽化바륨을 鹽酸-醋酸에 녹인 混酸溶液을 定量試樂으로 使用함으로선 確立되었다. 黃酸바륨의 溶解度以下에 該當하는 微量 黃酸이온의 測定을 위해서는, 沈沈澱反應에 影響을 미치는 共通이온의 添加量. pH, 溫度, 溶媒 그리고 反應時間 等을 詳細히 檢討하여 얻어진 最適條件을 定量에 利用함으로서 黃酸바륨 및 크롬酸염바륨의 溶解度가 크게 減少혀었다. 特히, 混酸溶液試藥은 化學量論的으로 計劃된 過量의 重크롬酸이온 을 一定量의 바륨이온이 含有된 混酸溶液에 添加함으로서 準備되었다. 本法에 의하면, 水溶液中의 0-50 ppm 黃酸이온이 定量的으로 測定可能하다. A more rapid, correct and simple method for the microdetermination of sulfate ions in natural water was established by using potassium dichromate - barium chloride in the mixed acid solution of hydrochloric acid and acetic acid. To determine the sulfate ion of micro amounts corresponding to the one which is less than solubility of barium sulfate, the solubilities of barium sulfate and barium chromate were strongly suppressed by using the optimal conditions of addition amount of common ions, pH, temperature, solvents and reaction time which have influences on the precipitation reaction. Especially, the stoichiometrically designed excess amount of the dichromate ions was added to the mixed acid solution containing a fixed amount of barium ions for the preparation of the mixed acid solution reagent. By the proposed method, 0 to 50 ppm of sulfate ions in aqueous solution can be quantitatively determined.

웹 기반 가상대학에 있어서의 학습관리시스템

홍영표 진주여자전문대학 1998 論文集 Vol.21 No.-

In the informative society the classroom is not the only place for education and the given curriculum and texts are not the only educational contents. Education possible wherever the computer is installed. In the existing web-based cyber university, however, the contents of lecture are distributed at a time in advance, so level-based learning and education are impossible. Therefore, this study tries to solve problems through the feedback between the professors and the students by the interactive learning on the web, and suggests a system model which makes level-based learning possible. The suggested system model has the learning session and the evaluation session in each unit. After learning each unit, the learner evaluates him learning with the extracted questions from the database. When he does not reach the professor's expected scores, he repeats the same process. So, the learner can control the learning progress and thus level-based learning and evaluation become possible.