Interleukin-1B(1L-1B) polymorphisms and gastric mucosal levels of IL-Iβ cytokine in Korean patients with gastric cancer

Chang, Young-Woon,Jang, Jae-Young,Kim, Nam-Hoon,Lee, Jae Won,Lee, Hyo Jung,Jung, Woon Won,Dong, Seok-Ho,Kim, Hyo-Jong,Kim, Byung-Ho,Lee, Joung-Il,Rin Chang KYUNG HEE UNIVERSITY MEDICAL CENTER 2006 고황의학상 수상논문집 Vol.21-22 No.-

Interleukin-1B and IL-1 receptor antagonist gene polymorphisms are associated with an increased risk of gastric cancer (GC) in Caucasian populations. However, recent studies could not find any association between IL-1B-511T polymorphism and the risk of GC in Asians. We tested for an association between IL-1 loci polymorphisms with increased gastric mucosal levels of IL-1β and an increased risk of developing GC in a Korean population. Polymorphisms of IL-1A-889, IL-1B-31, IL-1B-511 and IL-1RN were genotyped in 434 controls and 234 patients with GC. Mucosal IL-1β cytokine was measured using an ELISA. The frequencies of IL-1A, IL-1B-511, IL-1B-31 and IL-1RN were not statistically different between controls and all patients with GC. After subclassification of GC, only patients with intestinal-type GC showed a higher frequency of IL-1B-31T homozygotes (OR = 2.2; 95% CI = 1.1-4.3) compared with controls. Risk was also significantly increased in these patients for IL-1B-31T homozygotes compared with patients with diffuse-type GC (OR = 3.4; 95% CI = 1.5-7.7). As in Caucasian populations, linkage disequilibrium between IL-1B-31 and IL-1B-511 was nearly complete, but the pattern of haplotype related to the risk of GC (IL-1B-31T/IL-1B-511C) was opposite (IL-1B-511T/IL-1B-31C). Mucosal IL-1β levels in H. pylori-infected GC patients were higher in patients homozygous for IL-1B-31T compared with IL-1B-31C/T and IL-1B-31C/C. Thus, the combined effects of H. pylori infection and IL-1B-31T/IL-1B-511C polymorphisms with enhanced mucosal IL-1β production contributed to the development of intestinal-type GC in this Korean population.

간장 ( 肝腸 ) 및 담도 ( 膽道 ) : B형 활동성 간염 환자에서 단기 Prednisolone과 ARA - AMP 겸용 치료의 경과에 따른 면역 지표의 변화에 관한 연구

민영일(Young Il Min),장린(Rin Chang),김병호(Byung Ho Kim),장영운(Young Woon Chang),이정일(Joung Il Lee),황이숙(Yi Sook Hwang),김정원(Jeong Won Kim),김효종(Hyo Jong Kim) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.3

N/A Withdrawal of corticosteroids in patients with chronic type B hepatitis is frequently associated with enhanced cellular immune response to hepatitis B virus. This immunolgic rebound generally results in a transient reduction in levels of HBV-DNA and DNA polymerase, and seldom results in clearance of HBeAg. There was a pilot study that the combination of a short course of prednisone followed by ARA-AMP was potentially synergistic. The object of this study was to determine the effect of short term prednisolone followed by ARA-AMP on the immune system in patients with chronic active ltepatitis-B. Patients were started 40 mg of prednisolone daily for 4 weeks. After prednisolone therapy was discontinued, patients received no medication for 2 weeks. ARA-AMP was then given for 4 weeks at a dose of 500 mg daily. Immune parameters such as T cell subsets, IL, production, NK cell activeity, and LAK cell activity were tested before and during treatement period. The resullts were as follows. 1) Basal IL2 production in CAH-B (71.3 unit/ml) was lower than that of normal control (p<0.025). Basal NK activity, T4/T8 ratio and LAK activity of CAH-B were similar to those of normal control. 2) After prednisolone treatment, IL2 production, NK cell activity and LAK cell activity were significantly lower than those of basal value. 3) After induction of immune rebound, IL, production increased to 84.8 unit/ml (basal 71.3 unit/ml,

Immune-Enhancing Effect of Nanometric Lactobacillus plantarum nF1 (nLp-nF1) in a Mouse Model of Cyclophosphamide-Induced Immunosuppression

( Dae-woon Choi ),( Sun Young Jung ),( Jisu Kang ),( Young-do Nam ),( Seong-il Lim ),( Ki Tae Kim ),( Hee Soon Shin ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.2

Nanometric Lactobacillus plantarum nF1 (nLp-nF1) is a biogenics consisting of dead L. plantarum cells pretreated with heat and a nanodispersion process. In this study, we investigated the immune-enhancing effects of nLp-nF1 in vivo and in vitro. To evaluate the immunostimulatory effects of nLp-nF1, mice immunosuppressed by cyclophosphamide (CPP) treatment were administered with nLp-nF1. As expected, CPP restricted the immune response of mice, whereas oral administration of nLp-nF1 significantly increased the total IgG in the serum, and cytokine production (interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-α)) in bone marrow cells. Furthermore, nLp-nF1 enhanced the production of splenic cytokines such as IL-12, TNF-α, and interferon gamma (IFN-γ). In vitro, nLp-nF1 stimulated the immune response by enhancing the production of cytokines such as IL-12, TNF-α, and IFN-γ. Moreover, nLp-nF1 given a food additive enhanced the immune responses when combined with various food materials in vitro. These results suggest that nLp-nF1 could be used to strengthen the immune system and recover normal immunity in people with a weak immune system, such as children, the elderly, and patients.

Comparison of Cytokine and Nitric Oxide Induction in Murine Macrophages between Whole Cell and Enzymatically Digested Bifidobacterium sp. Obtained from Monogastric Animals

Dong Woon Kim,Sung Back Cho,Hyun Jeong Lee,Wan Tae Chung,Kyoung Hoon Kim,Jong Hwangbo,In Sik Nam,Young Il Cho,양만표,Il Byung Chung 한국미생물학회 2007 The journal of microbiology Vol.45 No.4

The principal objective of this study was to compare the effects of whole and hydrolyzed cells(bifidobacteria) treated with gastrointestinal digestive enzymes on the activation of cloned macrophages. Seven different strains of Bifidobacterium obtained from swine, chickens, and rats, were digested with pepsin followed by pancreatin and the precipitate (insoluble fraction) and supernatant (soluble fraction) obtained via centrifugation. The RAW 264.7 murine macrophages were incubated with either whole cells, the precipitate, or supernatant at various concentrations. Pronounced increases in the levels of nitric oxide (NO), interleukin (IL)-1β, IL-6, IL-12, and tumor necrosis factor (TNF)-α were observed in the whole cells and precipitates, but these effects were less profound in the supernatants. The precipitates also evidenced a slight, but significant, inductive activity for NO and all tested cytokines, with the exception of TNF-α in the macrophage model as compared with the whole cells. By way of contrast, TNF-α production when cultured with whole cells (100 ng/ml) resulted in marked increases as compared with what was observed with the precipitates. The results of this study indicated, for the first time, that digested Bifidobacterium sp. can induce the production of NO and several cytokines in RAW 264.7 murine macrophage cells. In the current study, it was demonstrated that Bifidobacterium strains treated with digestive enzymes, as compared with whole cells, are capable of stimulating the induction of macrophage mediators, which reflects that they may be able to modulate the gastrointestinal immune functions of the host.

Comparison of Cytokine and Nitric Oxide Induction in Murine Macrophages between Whole Cell and Enzymatically Digested Bifidobacterium sp. Obtained from Monogastric Animals

Kim, Dong-Woon,Cho, Sung-Back,Lee, Hyun-Jeong,Chung, Wan-Tae,Kim, Kyoung-Hoon,HwangBo, Jong,Nam, In-Sik,Cho, Yong-Il,Yang, Mhan-Pyo,Chung, Il-Byung The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.4

The principal objective of this study was to compare the effects of whole and hydrolyzed cells (bifidobacteria) treated with gastrointestinal digestive enzymes on the activation of cloned macrophages. Seven different strains of Bifidobacterium obtained from swine, chickens, and rats, were digested with pepsin followed by pancreatin and the precipitate (insoluble fraction) and supernatant (soluble fraction) obtained via centrifugation. The RAW 264.7 murine macrophages were incubated with either whole cells, the precipitate, or supernatant at various concentrations. Pronounced increases in the levels of nitric oxide (NO), interleukin $(IL)-1{\beta}$, IL-6, IL-12, and tumor necrosis factor $(TNF)-{\alpha}$ were observed in the whole cells and precipitates, but these effects were less profound in the supernatants. The precipitates also evidenced a slight, but significant, inductive activity for NO and all tested cytokines, with the exception of $(TNF)-{\alpha}$ in the macrophage model as compared with the whole cells. By way of contrast, $(TNF)-{\alpha}$ production when cultured with whole cells (100 ng/ml) resulted in marked increases as compared with what was observed with the precipitates. The results of this study indicated, for the first time, that digested Bifidobacterium sp. can induce the production of NO and several cytokines in RAW 264.7 murine macrophage cells. In the current study, it was demonstrated that Bifidobacterium strains treated with digestive enzymes, as compared with whole cells, are capable of stimulating the induction of macrophage mediators, which reflects that they may be able to modulate the gastrointestinal immune functions of the host.

Reduction of allergenicity of irradiated ovalbumin in ovalbumin-allergic mice

Seo, Ji-Hyun,Lee, Ju-Woon,Kim, Jae-Hun,Byun, Eui-Baek,Lee, Soo-Young,Kang, Il-Jun,Byun, Myung-Woo Elsevier 2007 Radiation physics and chemistry Vol.76 No.11

<P><B>Abstract</B></P><P>Egg allergy is one of the most serious of the immediate hypersensitivity reactions to foods. Such an allergic disorder is mediated by IgE antibodies stimulated by T-helper type 2 (Th2) lymphocytes. This study was undertaken to evaluate changes of allergenicity and cytokine profiles by exposure of irradiated ovalbumin (OVA), a major allergen of egg white, in the OVA-allergic mice model. OVA solutions (2mg/ml in 0.01M phosphate buffered saline (PBS) were gamma-irradiated to 50 and 100kGy. The allergenicity in the OVA-allergy-induced mice model was remarkably reduced when challenged with irradiated OVA. Cultures of spleen cells harvested from OVA-sensitized mice showed a significant decrease in Th2 cytokine levels of ILs-4 and -5 with a concomitant increase in Th1 cytokine levels of IL-12 when co-cultured with irradiated OVA. However, IFN-<I>γ</I> level decreased dependant on the radiation dose of co-cultured OVA. The levels of IgEs and Th2-cytokine were reduced dependant on the radiation dose. These data show that the irradiated OVA could downregulate the activity of Th2 lymphocytes in OVA-sensitized mice.</P>

Metagonimus Takahashii와 Heterophyes Nocens의 혼합인체감염 1례

손운목,고원규,조규일 인제대학교 1992 仁濟醫學 Vol.13 No.2

1991년 8원 16일 부산백병원 종합검진센타를 찾은 27세 남자의 대변에서 異形吸蟲類의 충란이 검출되었다. 한달후 parziquantel과 황산 마그네슘을 투여하여 치료하였던 바, 587 마리의 M. takahashii와 8 마리의 H. nocens가 검출되었다. 이에 생물학적, 역학적 性狀이 다른 두 이형흡충류의 혼합인체감염 1례를 보고함과 아울러 분류학적으로 이견이 많은 M. takahashii의 형태학적 특징을 재기술하였다. A case of natural human infection by Metagonimus takahashii and Heterophyes nocens was confirmed by collection of adult warms after praziquatel treatment on August, 1991, and in order to clarify the species identity, the morphological characteristics of M. takahashii were rediscribed. The patient was a 27-year old male residing in Hadong-gun, Kyungnam Province, Korea. He had the clinical complaints of fatigue, indigestion and arrhythmia, and said to have eaten raw flesh of several kinds of marine and/or freshwater fish. Praziquantel in single dose 10mg/kg was given followed by purgation with magnesium salt, and 587 M.takahashii and 8 H. nocens were collected from the diarrheal stools. M. takahashii were dorsoventrally flattened, 0.998×0.573mm in average size, and they had the following morphological characteristics : no posteriormost location of right testes ; separated left testis from the right one ; distribution of uterine tubules over the left testis and intertesticular junction ; distribution of the vitellaria passing through the posteriormost portion of the body ; larger size of the eggs. H. nocens were also dorsoventrally flattened, 0.924×0.543mm in average size, and they had a well-developed ventral sucker and a genital sucker bearing the 54 chitinous rodlets. From the above results, a case of human infection by M. takahashii and H. nocens was identified. This study may be the 32nd human case of H. nocens infection in the literature in Korea.

HFC-134a와 대체냉매 HFC-152a에 CF_3I를 혼합한 자동차냉매 특성

권일욱,박찬수,홍경한,문기선,최운수,서의경,윤갑식,이종인,하옥남 朝鮮大學校 機械技術硏究所 2002 機械技術硏究 Vol.5 No.1

These days, an attention of environment has been increasing all over the world. to prevent the green house effect and destruction of an ozone layer, an ozone destruction potential(ODP) be zero and a refrigerant for low global warming potential(GWP) is needed. HFC-134a, in which hydrogen is mixed instead of chlorine is a refrigerant used for automobile conditioners and its destruction potential is ecologically zero. However, it is not considered as a perfect substitutive refrigerant as its GWP is high. It is studied refrigerant mixtures in which HFC-134a, HFC-152a and CF_3I with low GWP and zero ODP are mixed by experimentally and concluded as follows; 1. with the variation of speed of compressor outside temperature and flow rate, the heat of evaporator and compressor and coefficient of performance was varied, and influenced the air conditioner. 2. The pressure of evaporator was decreased with increasing the speed of compressor and that of the refrigerant HFC-134a was higher 21% than that of azotrope refrigerant mixed with CF_3I. The overall capacity of condensor and evaporator was increased and HGC-134a was higher than azotrope refrigerant mixed with CF_3I 3. The capacity of evaporator with the refrigerant HFC-134a was higher 6% than azotrope refrigerant mixed with CF31 and the required power with zaotrope refrigerant mixed with CF_3I was higher 11% than that of HFC-134a. 4. The COP of the refrigerant FHC-134a was higher 31% in 1000RPM. when outside temperature was increased, COP was decreased. 5. Performance of HFC-134a is better than azotrope refrigerant mixed with CF_3I but prefered zaotrope refrigerant mixed with CF_3I considering the environment.

木浦·長興地方의 降雨强度式 誘導

金雲中,池光一,鄭南先 朝鮮大學校 建設技術硏究所 1999 建設技術硏究 Vol.19 No.1

In hydrology, rainfalls apply to mean every type of rains which were dropped from clouds and condensed into waters. While the rains made from during the condensing process are studied in meteorology, the raindrops on the ground are the subject of hydrology. For background knowledge, this study has collected research materials and data on rainfalls for 20years in Mok-Po and Chang-Hung. Then, by surveying the existing articles and analysing experimental records, this paper tried to predict the accurate amount of the rainfalls for the limited time. The methods used for foretelling rainfalls optimal probability are as follows; Normal Distribution Methods, Log Normal Distribution Method, Moment Method, Gumbel-Chow Method, Iwai Method are applied. The methods for significant statistic results are as follows; Talbot, Sherman, Japanese and Semi-log are used. The computer was used to figure out the invariable number for the methods while the most proper formula was selected through comparing χ ²-test number and standard reduction rate. The formulas for rainfall optimal probability are as follows; In Mok-Po, Normal Distribution Method is used for 3, 5, 10, 200 years and Gumbel-Chow is taken into account for 20, 30, 50, 100 years. In Chang-Hung, Gumbel-Chow was used for 3 years and thanks to the smallest error, Iwai was used for more than 5 years. After comparing standard error with χ ²-test number, Japanese was appropriate in Mok-Po for 3, 5 years and Sherman for over 10 years. In Chang- Hung, Japanese was most useful from 3 to 20 years and Sherman for over 30 years. In this study, the formulas suggested for optimal probability have 97.24% significancy for Mok-Po and 97.83% significancy for Chang-hung. Therefore, the offered formulas are meaningful to consider in constructing hydrological buildings in that they have higher optimal probability than the existing ones and they have similar predictive results with ones by meteorological observatory. The formulas offered in this study are expected to have significancy according to rainfall frequency, rainfall duration and rainfall intensity. Furthermore, they have useful value related with I.D.F correlation.

Kant에 있어서 "純粹悟性槪念의 演繹"은 成功 하였는가?

崔逸雲 全北大學校 1957 論文集 Vol.1 No.-

주지하는 바이지만 Kant는 그의 철학의 입장을 코페루니크스의 사고방식에서 찾았고 또 그러한 입장에서 순수이성비판의 전체계를 주었던 것이다. 그에 의하면 우리의 인식이 대상에 의준(richten sieh nach)하는 것이 아니라, 대상이 우리의 인식에 의준한다고 본다. 이것은 실로 관념론과 실존론의 분기점에서 관념론에로 떨어지는 제1보였지만 보다도 Kant가 이러한 입장에 서서 시간과 공간을 주관적대상으로볼 뿐아니라, 순수오성개념 즉 범주까지도 그러한것으로 본것은 우리로서는 십분 주의가 요하는 점이다. 이에 있어서 필연적으로 문제가 되지 않을 수 없는 것이 주관적대상으로서 이러한 오성개념이 어떻게 하여 객관에 타당할수가 있는가 하는 것이다. 이것이 해명되지 않는다면 순수오성개념이란 하나의 허구에 지나지 못하는 것이되고 만다. Kant는 그의 당연한 이론적결과로서 이 문제에 봉착하였던 것이다.