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Frequency spectra characterization of noncoding human genomic sequences
O. Paredes,Rebeca Romo‑Vázquez,Israel Román‑Godínez,Hugo Vélez‑Pérez,Ricardo A. Salido‑Ruiz,J. Alejandro Morales 한국유전학회 2020 Genes & Genomics Vol.42 No.10
Background Noncoding sequences have been demonstrated to possess regulatory functions. Its classifcation is challenging because they do not show well-defned nucleotide patterns that can correlate with their biological functions. Genomic signal processing techniques like Fourier transform have been employed to characterize coding and noncoding sequences. This transformation in a systematic whole-genome noncoding library, such as the ENCODE database, can provide evidence of a periodic behaviour in the noncoding sequences that correlates with their regulatory functions. Objective The objective of this study was to classify diferent noncoding regulatory regions through their frequency spectra. Methods We computed machine learning algorithms to classify the noncoding regulatory sequences frequency spectra. Results The sequences from diferent regulatory regions, cell lines, and chromosomes possessed distinct frequency spectra, and that machine learning classifers (such as those of the support vector machine type) could successfully discriminate among regulatory regions, thus correlating the frequency spectra with their biological functions Conclusion Our work supports the idea that there are patterns in the noncoding sequences of the genome.
Henrique Inhauser Riceti Magalhã,es,Jeferson Borges Barcelos,Fabiano Braz Romã,o,Tâ,nia Ribeiro Junqueira Borges,Roseâ,mely Angé,lica de Carvalho-Barros,Maria Angelica Miglino,Frederico Ozanam Carneir 대한해부학회 2021 Anatomy & Cell Biology Vol.54 No.2
Considering Suidae Familie as a perfect and viable experimental biomedical model for research applied to human medicine, it has been sought to describe the comparative anatomy of the digastric and the stylohyoid muscles between boars and domestic swine. Heads of Sus scrofa scrofa and Sus scrofa domesticus were dissected. The digastric muscle presented only one muscle belly as anatomical component of a tendinous origin in the jugular process of the occipital bone, and muscle insertion in the midventral edge of the caudal two thirds of the body of the mandible. Thus, its function is fundamentally associated with the lowering and the retracting of the mandible which, by the way, can deliver greater muscle power at lesser energy expense. For the stylohyoid muscle, the tendinous origin was in the laterocaudal edge of the dorsal third of the stylohyoid bone. The muscle insertion - primarily, was in the lateral and caudal edges from the mid third portion up to the ventral extremity of the thyrohyoid bone, and secondarily as a laterolateral aponeurotic blade which would unite, in a bilateral manner, an insertion that was common to the sternohyoid, the geniohyoid, and the mylohyoid muscles in a median ventral region. This morphology were similar to the two specimens studied expanding the information available, which were completely unknown for the suidae until the moment.
Tiago P. Freitas,Paulo C. Silveira,Luís G. Rocha,Gislaine T. Rezin,João Rocha,Vanilde Citadini-Zanette,Pedro T. Romão,Felipe Dal-Pizzol,Ricardo A. Pinho,Vanessa M. Andrade,Emilio L. Streck 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.4
Several studies have reported biological effects of Mikania glomerata and Mikania laevigata, used in Brazilian folk medicine for respiratory diseases. Pneumoconiosis is characterized by pulmonary inflammation caused by coal dust exposure. In this work, we evaluated the effect of pretreatment with M. glomerata and M. laevigata extracts (MGE and MLE, respectively) (100 mg/kg, s.c.) on inflammatory and oxidative stress parameters in lung of rats subjected to a single coal dust intratracheal instillation. Rats were pretreated for 2 weeks with saline solution, MGE, or MLE. On day 15, the animals were anesthetized, and gross mineral coal dust or saline solutions were administered directly in the lung by intratracheal instillation. Fifteen days after coal dust instillation, the animals were killed. Bronchoalveolar lavage (BAL) was obtained; total cell count and lactate dehydrogenase (LDH) activity were determined. In the lung, myeloperoxidase activity, thiobarbituric acid-reactive substances (TBARS) level, and protein carbonyl and sulfhydryl contents were evaluated. In BAL of treated animals, we verified an increased total cell count and LDH activity. MGE and MLE prevented the increase in cell count, but only MLE prevented the increase in LDH. Myeloperoxidase and TBARS levels were not affected, protein carbonylation was increased, and the protein thiol levels were decreased by acute coal dust intratracheal administration. The findings also suggest that both extracts present an important protective effect on the oxidation of thiol groups. Moreover, pretreatment with MGE and MLE also diminished lung inflammatory infiltration induced by coal dust, as assessed by histopathologic analyses. The present study indicates that M. glomerata and M. laevigata might become good candidates for the prevention of lung oxidative injury caused by coal dust exposure.
Effect of repair methods and materials on the flexural strength of 3D-printed denture base resin
Hamile Emanuella do Carmo Viotto,Marcela Dantas Dias Silva,Thaís Soares Bezerra Santos Nunes,Sabrina Romão Gonçalves Coelho,Ana Carolina Pero 대한치과보철학회 2022 The Journal of Advanced Prosthodontics Vol.14 No.5
PURPOSE. The aim of this study was to evaluate the flexural strength of a 3D-printed denture base resin (Cosmos Denture), after different immediate repair techniques with surface treatments and thermocycling. MATERIALS AND METHODS. Rectangular 3D-printed denture base resin (Cosmos Denture) specimens (N = 130) were thermocycled (5,000 cycles, 5°C and 55°C) before and after the different repair techniques (n = 10 per group) using an autopolymerized acrylic resin (Jet, J) or a hard relining resin (Soft Confort, SC), and different surface treatments: Jet resin monomer for 180 s (MMA), blasting with aluminum oxide (JAT) or erbium: yttrium-aluminum-garnet laser (L). The control group were intact specimens. A three-point flexural strength test was performed, and data (MPa) were analyzed by ANOVA and Games-Howell post hoc test (α = 0.05). Each failure was observed and classified through stereomicroscope images and the surface treatments were viewed by scanning electron microscope (SEM). RESULTS. Control group showed the highest mean of flexural strength, statistically different from the other groups (P < .001), followed by MMA+J group. The groups with L treatment were statistically similar to the MMA groups (P > .05). The JAT+J group was better than the SC and JAT+SC groups (P < .05), but similar to the other groups (P > .05). Adhesive failures were most observed in JAT groups, especially when repaired with SC. The SEM images showed surface changes for all treatments, except JAT alone. CONCLUSION. Denture bases fabricated with 3D-printed resin should be preferably repaired with MMA+J. SC and JAT+SC showed the worst results. Blasting impaired the adhesion of the SC resin.
Almeida Jonathan Mádson dos Santos,Pascoal Leonardo Augusto Fonseca,de Almeida Jorge Luiz Santos,Guerra Ricardo Romão,da Silva José Humberto Vilar,da Silva David Rwbystanne Pereira,Silva Neto Manoel R 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12
Objective: The present study aimed to evaluate the influence of including L-glutamine along with glutamic acid as a supplement in weaned piglets’ diets with and without whey powder. Methods: Two assays were carried out. A total of 40 piglets ([Landrace×Large White]× Pietrain) weaned at 24 days of age with an initial body weight of 6.6±0.6 kg were used in the first assay, and the following parameters were evaluated: growth performance, the incidence of diarrhea, morphometry, intestinal integrity, and hepatic glycogen index. The animals were then blocked into four groups according to different diets: diet all-grain feeding (G); diet all-grain feeding with whey powder (GW); and with vs without 1% supplementation of the commercial product containing L-glutamine and glutamic acid (A or NA). Whey powder was added according to the stage of life, corresponding to 17%, 10%, and 5%, respectively, in order to meet the need for lactose. The animals were evaluated at 24 to 42 days and at 24 to 55 days of age. The nutrient digestibility for the second assay was carried out by using 24 animals with an average weight of 11.49±1.6 kg, and the same diets were tested. Results: The supplementation of L-glutamine + glutamic acid or the addition of whey powder in diets for weaned piglets provided (p<0.05) greater feed intake, greater weight gain and improved feed conversion in the initial period (24 to 42 days age). However, in the whole period (24 to 55 days age) only amino acid supplementation affected (p<0.05) growth performance. There was a positive interaction (p<0.05) between the type of diet and L-glutamine + glutamic acid supplementation on villus height, crypt depth and the villus:crypt ratio in the duodenum. In addition, L-glutamine + glutamic acid supplementation reduced (p<0.05) the crypt depth and improved the villus:crypt ratio in the jejunum. The inclusion of whey powder affected (p<0.05) positively the digestibility coefficients analyzed except mineral matter digestibility coeficients. The supplementation of 1% the commercial product composed of L-glutamine and glutamic acid improved (p<0.05) only the digestibility coefficient of crude protein. Conclusion: These results indicate that supplementation of 1% commercial product containing L-glutamine + glutamic acid in diets for piglets from 24 to 55 days of age, dispenses with the use of whey powder when evaluating growth performance. Amino acid supplementation alone or associated with whey powder affects (p<0.05) positively the indicators of the intestinal integrity. Objective: The present study aimed to evaluate the influence of including L-glutamine along with glutamic acid as a supplement in weaned piglets’ diets with and without whey powder.Methods: Two assays were carried out. A total of 40 piglets ([Landrace×Large White]× Pietrain) weaned at 24 days of age with an initial body weight of 6.6±0.6 kg were used in the first assay, and the following parameters were evaluated: growth performance, the incidence of diarrhea, morphometry, intestinal integrity, and hepatic glycogen index. The animals were then blocked into four groups according to different diets: diet all-grain feeding (G); diet all-grain feeding with whey powder (GW); and with vs without 1% supplementation of the commercial product containing L-glutamine and glutamic acid (A or NA). Whey powder was added according to the stage of life, corresponding to 17%, 10%, and 5%, respectively, in order to meet the need for lactose. The animals were evaluated at 24 to 42 days and at 24 to 55 days of age. The nutrient digestibility for the second assay was carried out by using 24 animals with an average weight of 11.49±1.6 kg, and the same diets were tested.Results: The supplementation of L-glutamine + glutamic acid or the addition of whey powder in diets for weaned piglets provided (p<0.05) greater feed intake, greater weight gain and improved feed conversion in the initial period (24 to 42 days age). However, in the whole period (24 to 55 days age) only amino acid supplementation affected (p<0.05) growth performance. There was a positive interaction (p<0.05) between the type of diet and L-glutamine + glutamic acid supplementation on villus height, crypt depth and the villus:crypt ratio in the duodenum. In addition, L-glutamine + glutamic acid supplementation reduced (p<0.05) the crypt depth and improved the villus:crypt ratio in the jejunum. The inclusion of whey powder affected (p<0.05) positively the digestibility coefficients analyzed except mineral matter digestibility coeficients. The supplementation of 1% the commercial product composed of L-glutamine and glutamic acid improved (p<0.05) only the digestibility coefficient of crude protein.Conclusion: These results indicate that supplementation of 1% commercial product containing L-glutamine + glutamic acid in diets for piglets from 24 to 55 days of age, dispenses with the use of whey powder when evaluating growth performance. Amino acid supplementation alone or associated with whey powder affects (p<0.05) positively the indicators of the intestinal integrity.
Genotoxic Evaluation of Mikania laevigata Extract on DNA Damage Caused by Acute Coal Dust Exposure
Tiago P. Freitas,Vanina D. Heuser,Priscila Tavares,Daniela D. Leffa,Gabriela A. da Silva,Vanilde Citadini-Zanette,Pedro R.T. Romão,Ricardo A. Pinho,Emilio L. Streck,Vanessa M. Andrade 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.3
In the present article, we report data on the possible antigenotoxic activity of Mikania laevigata extract (MLE) after acute intratracheal instillation of coal dust using the comet assay in peripheral blood, bone marrow, and liver cells and the micronucleus test in peripheral blood of Wistar rats. The animals were pretreated for 2 weeks with saline solution (groups 1 and 2) or MLE (100mg/kg) (groups 3 and 4). On day 15, the animals were anesthetized with ketamine (80mg/kg) and xylazine (20mg/kg), and gross mineral coal dust (3mg/0.3mL saline) (groups 2 and 4) or saline solution (0.3mL) (groups 1 and 3) was administered directly in the lung by intratracheal administration. Fifteen days after coal dust or saline instillation, the animals were sacrificed, and the femur, liver, and peripheral blood were removed. The results showed a general increase in the DNA damage values at 8 hours for all treatment groups, probably related to surgical procedures that had stressed the animals. Also, liver cells from rats treated with coal dust, pretreated or not with MLE, showed statistically higher comet assay values compared to the control group at 14 days after exposure. These results could be expected because the liver metabolizes a variety of organic compounds to more polar by-products. On the other hand, the micronucleus assay results did not show significant differences among groups. Therefore, our data do not support the antimutagenic activity of M. laevigata as a modulator of DNA damage after acute coal dust instillation.