합병증이 동반된 Meckel씨 게실 2례 보고

정병욱,박동일,이준희,서정욱,원유홍,김웅,신현종,권영무 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

합병증이 동반된 Meckel씨 게실을 가진 환자의 치료에 대한 필요성은 과정이 문제이다. 하지만 우연히 만나는 게실의 처치는 논란 중이다. 부수적인 게실 절제술(incidental diverticulectomy)의 제안자는 증상이 있는 게실의 외과적 처치는 중요한 이환 및 사망률은 지적한다. 이를 반대하는 사람들은 전 생애를 통해 합병증의 위험성이 낮게 나타나므로 필요가 없다고한다. 게실의 예방적 제거는 개복술시 우연히 발견 될 때 시행한다. 이는 남아있는 게실이 나중에 합병증을 일으키는 것보다 술후 합병증의 위험성이 매우 적음을 알 수 있다. 저자들은 합병증이 동반된 Meckel씨 게실 2예를 경험하였기에 문헌 고찰과 더불어 보고하는 바이다. The necessity for therapy with complicated Meckel's diverticular is a matter of course.However, management of the incidentally encountered diverticulum is controversial. Proponents of incidental diverticulectomy point to the significant morbidity and mortality rates of surgical treatment of symptomatic divenicular. Other authors base their opposition to incidental diveniculectomy on the low risk of complications developing during lifetime. Prophyillctic removal of the diveniculum is indicated when it is found incidentally at laparotomy, because there are few postoperative complications and the risk exists that the remaining diverticulum may be the cause of later complication. The 2 cases of complicated Meckel's diverticulum are reported with a brief review of literature.

Welcoming Address

( Ook Joon Yoo ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Transcriptional Activities of Two Different forms of Human TFIIS

Yoo, Ook Joon,Cho, Nam Young,Hahn, Bum Soo,Baek, Kwang Hee,Park, Heung Rok 한국유전학회 1993 Genes & Genomics Vol.15 No.2

Transcription Factor IIS(TFIIS) exerts influence on the transcript elongation by RNA polymerase II. Two different cDNA clones of human TFIIS have been found. If we compare the amino acid sequence in the coding region of these two clones, we can find one of the cDNA clone carry extra 21 amino acids at the N-terminal region of TFIIS. To identify the functional role of these additional amino acid sequence, we have expressed and purified two different forms of TFIIS in E. coli. The results of the comparison between these two proteins in the transcription elongation activities indicate that 21 amino acid sequence is not crucial for the function of TFIIS in our in vitro assay system. But we cannot rule out the possibility of their functional role(s) in vivo.

Monoclonal Antibody CFC - 6 , which Binds to Helix Ⅱ , Inhibits Erythropoietin - Induced Bioactivity

Kim, Suk Joon,Yoo, Ook Joon,Ha, Byung Jhip,Oh, Myung Suk,Kim, Hyun Su,Yoo, Ree Ann,Lee, Dong Eok,Park, Ji Sook,Woo, Koo 생화학분자생물학회 1998 BMB Reports Vol.30 No.5

It was discovered that monoclonal anti -erythropoietin (EPO) antibody CFC-6 can neutralize EPO-induced cell activation. To know the binding site of CFC-6, recombinant human erythropoietin (rhEPO) was digested with Glu-C. followed by a separation using high performance liquid chromato graphy (HPLC). Each HPLC fraction was blotted on the nitrocellulose membrane and the membrane was treated with anti-EPO antibody CFC-6 and anti-mouse antibody which is modified with peroxidase. Only one spot showed the color and the fraction was sequenced by Edman degradation. The results suggest that CFC-6 recognizes amino acid sequence V63-W-Q-G-L-A-L-L-S-E72 which is a part of helix II of the EPO molecule. Binding of CFC-6 to EPO may inhibit EPO binding to its receptor. which implies that the antibody binding site and the receptor binding site are close or overlapping.

만성간질환에서 당부하시험

정준모 ( Joon Mo Chung ),최용환 ( Yong Hwan Choi ),김대환 ( Dae Whan Kim ),김제형 ( Jae Hyung Kim ),정영채 ( Young Chae Jung ),김학현 ( Hak Hyun Kim ),허성욱 ( Seong Ook Huh ) 대한내과학회 1984 대한내과학회지 Vol.27 No.3

안면거상술(Face Lift)을 이용한 안면노화(Aging face) 치험례(319례)

최 준,신명수,김동일,이영준,백세민,오재욱 大韓成形外科學會 1992 Archives of Plastic Surgery Vol.19 No.6

There are some differences between Orientals and Caucasians in the amount and type of aging. In general, Orientals have thicker facial skin with more subcutaneous adipose tissues than Caucasians. These differences result in characteristic Oriental aging face combined with round and flat facial contours. Many surgical techniques of face lift or rhytidectomy have been largely performed in Caucasians, but less in Orientals, therefore, we should consider modified principles and techniques in the surgery of the oriental aging face. First, postoperative incision scars are more prominent in Orientals than in Caucasians and this calls for a modified incision design n oriental patients. Second, wide dissection is mandatory for a good result because the skin is very thick and less pliable in oriental patients. Third, it is not necessary to perform cervicofacial platysmal surgery in most cases of oriental face lift. We experienced 171 cases of oriental patients with aging faces and performed 319face lifting procedures during recent 3 years(from Mar. 1988 to Feb. 1991). Combined procedures such as suction lipectomy, forehead augmentation, blepharoplasty, and facial bone contouring surgery were performed simultaneously case by case. Types of anesthesia were decided according to the psychologic states and wishes of patients and the types of combined procedures. Thirty-five cases of 171 patients(20.5%) were performed under local anesthesia. Subcutaneous forehead lifts with ultrasobic vibrator-dissection were our new interesting trials with good results. Complications were 11 cases of wound hematoma(6.4%) and 3 cases of focal wound dehiscence(1.8%). The patients with the complications were completely recovered 6 months later.

Researches on Restriction - Modification Enzymes

유욱준 한국유전학회 1986 Genes & Genomics Vol.8 No.1

1. Large scale preparation of Stu I endonuclease One million units of homogeneous Stu I endonuclease have been isolated from 100g (wet weight) of Streptomyces tubercidicus cells. For the purification of this enzyme, DEAE-Sephadex (A-50), QAE-Sephadex (A-50) and Heparin-Agarose column chromatography have been performed after ammonium sulfate fractionation of the crude extract. The subunit molecular weight was 34,000 dalton as judged by 10% SDS-polyacrylamide gel electrophoresis. Stu I endonuclease requires Mg^(2+) for its activity and shows maximum activity at pH 7.0-8.0 in the absence of NaCl. 2. Zan I endonuclease: An Isoschizomer of Eco RII and Bst NI endonuclease A new sequence specific endonuclease, Zan I endonuclease, from Zymomonas anaerobia has been purified to homogeneity. The subunit molecular weight was 31,000 dalton as judged by 10% SDS-polyacrylamide gel electrophoresis. The endonucleolytic cleavage products of various DNA substrates showed that Zan I endonuclease is an isoschizomer of Bst NI and Eco RII endonucleases. The conditions for the maximum activity of this enzyme were determined which is different from those of Bst NI and Eco RII endonuclease. 3. DNA protection by Alu I, Hpa II and Hha I Methylases Hexameric restriction endonucleases can be inhibited by the methylation on the internal tetrameric sequences. The degree of inhibitions by Alu I, Hpa II and Hha I methylases allow us to predict the specificities of various hexameric restriction methylases which have never been isolated. On the other hand, the results could be the important informations for the cDNA cloning experiments using restriction linker DNAs.

Acid Stable Trypsin Inhlbitor 에 대한 단일클론 항체의 제조와 위암조직에서의 발현양상

유욱준,정연태,문형배,김정중,이황희,장원희,노종섭,최완성 한국유전학회 1994 Genes & Genomics Vol.16 No.4

Monoclonal antibodies against human acid stable trypsin inhibitor (ASTI) was produced by hybridizing SP2/0-Ag14 mouse myeloma cells with spleen cells of Balb/c mouse immunized with ASTI. Western blotting analysis using purified ASTI and urinary proteins exhibited the specificity of the antibodies agains ASTI. The distribution and localization of ASTI in adenocarcinoma of stomach were examined using immunohistochemical techniques. ASTI immunoreactivity was detected from the tubular adenocarcinoma, but not from the signet ring cell carcinoma or mucinous adenocarcinoma.

토론토대학교 구조실험실

김욱종(Ook-Jong Kim),김형준(Hyung-Joon Kim) 한국콘크리트학회 2009 콘크리트학회지 Vol.21 No.2

다양한 유리피판술을 이용한 비대칭 안면부의 치험례

최준,백롱민,오재욱,윤용현,백세민,정영덕 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.5

People with asymmetric face have many handicaps in their social life. Asymmetric face with soft tissue deficiency can be corrected by multiple autogenous tissue transfer. Between November of 1988 and June of 1992, authors have corrected asymmetric faces with various free flaps(n=16) with excellent results. In five cases, facial bone surgeries have been performed to correct skeletal asymmetry before free flap surgery. Deepithelized groin free flaps were used most commonly(n=11). Superficial temporal and facial vessels were used recipient vessels. Authors consider that free flap surgery is the best choice for the correction of various degree of facial depression deformity. It has several advantages as below. 1. It is one-stage operation and simpler than local or distant flap method. 2. The patients were comfortable and relieved from their long term hospitalization and disability. 3. Various types of free flap can be used according to the size of defect and the length of vascular pedicle.