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폐의 소세포암종에서 Synaptophysin, Neuron-SpecificEnolase, Chromogranin A, Neurofilarnent 항체들의 감수성에 대한연구
노종섭 ( Jong Sup Roh ),왕성수 ( Sung Su Wang ),이정아 ( Chung A Lee ),곽효일 ( Hyo Il Kwak ),윤기중 ( Ki Jung Yun ) 대한임상검사과학회 1998 대한임상검사과학회지(KJCLS) Vol.30 No.3
A variety of benign and malignant tumors may arise in the lung, but the bronchogenic carcinoma is majority. Among the brochogenic carcinomas, small cell carcinoma is a histologically distinctive type, and it is higly malignant and chemosensitive To evaluate of sensitivity of neuroendocrine markers such as synaptophysin, neuron-specific enolase(NSE), chrorrogranin A and neurofilament antibodies, we studied the small cell carcinoma of lung on the 32 paraffin embedded tissues. The sensitivities of synaptophysin, NSE, chrorrogranin A and neurofilarrent were ``irl.5%, 31.30/0, 25%, and l000~ respectively, Above result indicates that neurofilament and synaptophysin antibodies are good for diagnosis of small cell carcinoma of the lung.
일밤 파라핀 절편에서 wet autoclave 전처리 후 AgNORs의 검출
제갈승주,곽효일,노종섭,이형섭 대한임상검사과학회 1997 대한임상검사과학회지(KJCLS) Vol.29 No.1
In the last few years the AgNORs technique has been used in tumor pathology for both diagnostic and prognostic patposes, but the lack of a standardizied staining protocol has frequently let to misinterpretation of actual structures evaluated by the various authors. The aim of the present study set out to measure the effect of wet autoclave pretreatment of routinely form alin -fixed and paraffin -embedded tissues on AgNORs staining. AgNOR quantity was evaluated n archival tumor tissues of 5 invasive adenocarcinoma of stomach , 8 invasive ductal carcinoma of breast and 14 invasive squamous cell carcinoma of uterine cervix. The treatment of sections at superheatedsteam temperature(121 degrees C) before silver incubation leads to a significantly better discrimination of individu a1 AgNORs , especially in AgNOR aggregates of nucleolus.Counting "black dots" by eye revealed considerably higher AgNOR mean numbers pernucleus in all pretreated cases when compared with the respective untreated controls. Also, mean AgNOR numbers increased after wet autoclave pretreatment in a linear manner(pairwise comparison by means of Pearson coπelation coefficient: r=0.89 instomach cancer, r=0.86 in breast cancer, r=0.86 in cervix cancer). It is suggested that the wet autoclave-pretreated method in routinely formalin-fixed and paraffin-embedded archival materials is more sensitive in detecting NORs than the untreated AgNORs staining and may prove a useful altemative for application in tumor pathology. .
자궁경부상피의 악성변화에서 비만세포가 간질세포의 α-Smooth Muscle Actin 발현에 미치는 영향
제갈승주,최영자,이광주,노종섭,Jekal, Seung-Joo,Choi, Young-Ja,Lee, Kwang-Joo,Roh, Jong-Sup 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3
There is increasing evidence that stromal reaction in cancer has an important diagnostic and prognostic significance. The aim of our study is to analyze the relation between the increase in mast cell number and the expression CD34 and alpha-smooth muscle actin (${\alpha}$-SMA) in the stroma of cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC). We investigated a total of 29 CIN (1,2,3) and 21 SCC (microinvasive and invasive) specimens and compared the distribution of $CD34^+$ stromal cells, ${\alpha}-SMA^+$ cells, transforming growth factor-${\beta}1$ $(TGF-{\beta}1)^+$ cells, and the density of mast cells using immunohistochemistry with antibodies against CD34, ${\alpha}$-SMA, TGF-${\beta}1$, and c-Kit (CD117) respectively. Computerized image analysis was to evaluate the positive area (%) and density of the respective immunoreactive cells. In CIN $CD34^+$ cells were abundant in the stroma but no ${\alpha}-SMA^+$ cells were identified except the wall of blood vessels. $CD34^+$ cells were progressively decreased along the continuum from CIN 2 to microinvasive SCC and not observed in the stroma of invasive SCC. Whereas ${\alpha}-SMA^+$ cells were only observed in the stroma of microinvasive and invasive SCC. We found more intense TGF-${\beta}1$ expression in the increased mast cells in the stroma of invasive SCCs than that in the stroma of CIN. These results indicate that disappearance of $CD34^+$ stromal cells and appearance of ${\alpha}-SMA^+$ cells are associated with the stromal change of CIN to SCC and the transformation of $CD34^+$ stromal cells into ${\alpha}-SMA^+$ cells is mediated by TGF-${\beta}1$ secretions in the stromal mast cell of SCC.
Acid Stable Trypsin Inhlbitor 에 대한 단일클론 항체의 제조와 위암조직에서의 발현양상
유욱준,정연태,문형배,김정중,이황희,장원희,노종섭,최완성 한국유전학회 1994 Genes & Genomics Vol.16 No.4
Monoclonal antibodies against human acid stable trypsin inhibitor (ASTI) was produced by hybridizing SP2/0-Ag14 mouse myeloma cells with spleen cells of Balb/c mouse immunized with ASTI. Western blotting analysis using purified ASTI and urinary proteins exhibited the specificity of the antibodies agains ASTI. The distribution and localization of ASTI in adenocarcinoma of stomach were examined using immunohistochemical techniques. ASTI immunoreactivity was detected from the tubular adenocarcinoma, but not from the signet ring cell carcinoma or mucinous adenocarcinoma.
자궁경부상피의 악성변화에서 비만세포가 간질세포의 α-Smooth Muscle Actin 발현에 미치는 영향
제갈승주 ( Seung Joo Jekal ),최영자 ( Young Ja Choi ),이광주 ( Kwang Joo Lee ),노종섭 ( Jong Sup Roh ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3
There is increasing evidence that stromal reaction in cancer has an important diagnostic and prognostic significance. The aim of our study is to analyze the relation between the increase in mast cell number and the expression CD34 and alpha-smooth muscle actin (α-SMA) in the stroma of cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC). We investigated a total of 29 CIN (1,2,3) and 21 SCC (microinvasive and invasive) specimens and compared the distribution of CD34+ stromal cells, α-SMA+ cells, transforming growth factor-β1 (TGF-β1)+ cells, and the density of mast cells using immunohistochemistry with antibodies against CD34, α-SMA, TGF-β1, and c-Kit (CD117) respectively. Computerized image analysis was to evaluate the positive area (%) and density of the respective immunoreactive cells. In CIN CD34+ cells were abundant in the stroma but no α-SMA+ cells were identified except the wall of blood vessels. CD34+ cells were progressively decreased along the continuum from CIN 2 to microinvasive SCC and not observed in the stroma of invasive SCC. Whereas α-SMA+ cells were only observed in the stroma of microinvasive and invasive SCC. We found more intense TGF-β1 expression in the increased mast cells in the stroma of invasive SCCs than that in the stroma of CIN. These results indicate that disappearance of CD34+ stromal cells and appearance of α-SMA+ cells are associated with the stromal change of CIN to SCC and the transformation of CD34+ stromal cells into α-SMA+ cells is mediated by TGF-β1 secretions in the stromal mast cell of SCC.
자궁경부상피내종양과 침윤성 편평상피암종의 혈관신생에서 비만세포와 혈관내피성장인자의 발현
제갈승주 ( Seung Joo Jekal ),이정아 ( Jung Ah Lee ),노종섭 ( Jong Sup Rho ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.3
To determine the correlation between mast cells(MCs) and neoangiogenesis in the growth and progression of cervical cancer, we investigated mast cell density(MCD), microvessel density(MVD) and the expression of vascular epithelial growth factor(VEGF) in cervical intraepithelial neoplasia and invasive suqamous cell carcinoma of the uterine cervix. Forty-five cervical intraepithelial neoplasia(CIN I, II and III), 15 microinvasive carcinomas, 15 invasive squamous cell carcinomas and 20 normal cervical epithelia were included in this study. MCs were stained with anti-c-Kit antibody and alcian blue, microvessels with anti-factor VIII antibody and VEGF with anti-VEGF antibody. The adjacent fields of both normal and neoplastic epithelium were used for counting MCs and microvessels. Computerized image analysis was used to evaluate MCD and MVD. MCD and MVD were the mean numbers per 1mm2 counted in 5-10 high and low power fields respectively. In both c-Kit and alcian blue stained sections, MCD progressively increased along the continuum from CIN I to invasive squamous cell carcinoma(p<0.001). MVD increased significantly with cervical neoplasia progression, from CIN to invasive squamous cell carcinoma (p<0.001). In double c-Kit and Factor VIII-stained sections, MCs were mainly present in the areas adjacent to newly formed blood vessels. However, there were no significant differences in MCD and MVD between normal epithelum and CIN I. A strong correlation was also observed between MCD and MVD. In double VEGF and alcian blue-stained sections, VEGF was expressed in only MCs. Strong VEGF-positive MCs were particularly abundant around the tumorous region. Our results suggest that MCs may upregulate neoangiogenesis by VGEF secretion in the development and progression of cervical neoplasia
자궁경부의 편평상피내병소와 편평상피세포암종에서의 비만세포에 관한 연구
제갈승주 ( Seung Joo Jekal ),차현희 ( Hyun Hee Cha ),최영자 ( Young Ja Choi ),노종섭 ( Jong Sup Roh ),곽효일 ( Hyo Il Kwak ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
Mast cells have recently been found to be well correlated with the inhibition of invasiveness and metastasis in various cancer. This work aimed to investigate the number of mast cells and ratio of mast cell granulation in squamous intraepithelial lesions and squamous cell carcinoma of the cervix. Cervical biopsy specimens were divided into low grade squamous intraepithelial lesion(n=20), high grade squamous intraepithelial lesion(n=75) and squamous cell carcinoma(n=40) by The Bethesda System. The sections were cut from routinely formalin- f1xed and paraffin-embedded tissues and stained with 0.5% toluidine blue(pHO.5)-nuc1ear fast red. The mast cells were quantif1cated the numbers per square millimeter using a computerized image analysis system. Mean number of mast cells was signif1cantly higher in high grade squamous intraepithelial lesion than in low grade squamous intraepithelial lesion and signif1cantly lower in squamous cell carcinoma than in high grade squamous intraepithelial lesion(p<0.01). The percent of mast cell ganulation also was significantly higher in high grade squamous intraepithelial lesion than in low grade squamous intraepithelial lesion and significantly lower in squamous cell carcinoma than in high grade intraepithelial lesion (p<0.01). These results suggested that mast cell number and percent of mast cell granulation were to be correlated to the development from precancerous lesion to cervical cancerous lesion and may be useful as markers of cervical squamous cell carcinoma.