Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

四大江 流域의 洪水에 관한 硏究 : 漢江을 中心으로

吳敬玉 梨花女子大學校 師範大學 社會生活科 1983 綠友硏究論集 Vol.- No.25

하반신체형이 플레어스커트 형상에 미치는 영향 : 중년 여성을 대상으로 Centering around the middle aged woman

함옥상,도윤경 啓明大學校 生活科學硏究所 1997 科學論集 Vol.23 No.-

ABSTRACTThe purpose of this study is to compare and examine the form characters among the changing elements of the body shape by types with the shape of middle aged woman's lower half of the body, which is one of many factors causing the flared skirt figure to be changed, and to analyse the effects of these middle aged woman's lower half of the body with remarkable change of the body shape on the flared skirt figure by carring out the wearing test with the human body. (1) The number of node showed that the body shape, T1, with the medium prominence of hip indicated the greatest number of node in terms of the fabric with high drape. In addition, it showed that the larger the flat rate without the sag of hip, the more the increase in the number of node. (2) The volume of the front node showed that the less the prominence of the abdomen, the more the increase of the node volume, while of the node that less the prominence of hip, the more the increase the volume of node. (3) The group with the largest prominence of the abdomen and hip showed the biggest horizontal and vertical diameters, and the ratio of the horizontal to vertical diameter indicated that the less the sag of hip, the larger the ratio, therefore, which showed the larger spread of horizontal section to the right and left sides.

子宮外 妊娠의 診斷에 血淸 Beta-human Chorionic Gonadotropin 測定의 價値

金京玉 全南大醫大 1983 전남의대학술지 Vol.20 No.4

무용창작에 있어 효과적인 교육방법

이경옥 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

The purpose of effective education method in creating dance Is to develop infinite capacity of imagination as a general category. Therefore the course of creative work is based on more comprehensive understanding, though it has many complexities. Various art dances created now play roles as linking chain and provide productive form newly made and created with a basis of beauty. It is the most important prerequisite in creating dance for the form of work to confirm itself. This confirmation work and effective methodological presentation must be done by educators. Though it gives learners self-confusion, it must present a method to solve the problem and to unbind the linking chain. Also, the technique must be so simple that learners can utilize their bodies as tools of self-expression and especially, it must be proved exciting and valuable to students who choose dance as their own profession. In conclusion, the form and matter is flexible enough to give Individuals who has different degrees a chance to express. Educators and learners do their best to develop the transition period of experimental work into present and future oriented educational form through the effective educational method, which regards body language performed under the name of creative work as a tool of development.

老年期 女性의 體型別 Slacks 原型 硏究

朴惠敬,成玉相 啓明大學校 生活科學硏究所 1991 科學論集 Vol.17 No.-

This study aims at the new pattern drafting of slacks on the basis of changes in the body types and comfort of elderly women. Therefore, by making the elderly women over 60 years old the subject of this study, it analyzed body types through the body measurement and the silhouettes of photographs, produced the pattern by the measurement of limb surface changes in order to decide the extre space of the pattern draft, and compared the new pattern with conventional patterns through the sensory evaluation. 1. As the result of the physique analysis, the elderly women's body types were largely divided into four types-type A, nealy standard, type B, obesity, type C, S form with a bent back and a protruding abdomen, and type D, a bent waist. 2. As the result of the measurement of changes in the limb surface caused by the limb movement, this study dediced the extra space on the basis of M??, which showed the greatest change. 3. As a result of the pattern drafting and wearing test, by contrast of Rim's pattern, A type has 4-centimeter margin in waist circumference and 4 to 6-centimeter one in hip circumference; has one centimeternarrowed slacks width and back dart and one centimeter-lengthened crotch part, B type, using the standard item of abdominal length, instead of hip circumference and hip length, has 4-centimeter margin in waist circumference and 6 to 8-centimeter one in abdominal circumference;has one centimeter-narrowed slacks width and back dart and one centimeter-lengthened crotch length and slacks lengt), C type has 4-centimeter margin in waist circumference and 4 to 6-centimeter one in hip circumference; has one centimeter-narrowed slacks width and one centimeter-lengthened crotch part, and has one centimeter-lifted front waist height and 2.5-centimeterlowered back waist height, D type has 4-centimeter margin in waist circumference and 4 to 6-centimeter one in hip circumference; has one centimeter-narrowed slacks width and one centimeter-lengthened crotch part, and has 2-centimeter-lowered front waist height and one centimeterlifted back waist height. 4. As a result of the sensory evaluation for appearance, since it showed the significant difference(α<0.01) in more than 12 items in every type, the new pattern proved superior to the conventional patterns. In addition, as a result of the sensory evaluation for comfort, since it showed the significant difference in movements and body parts the new pattern proved also superior to the conventional pattern.

在韓 西歐人의 住居空間 使用 行態 및 要求 分析 : 현관, 욕실, 세탁실을 중심으로 Focus on the Entrance, Bathroom and Laundry

장상옥,신경주 대한건축학회 2004 대한건축학회논문집 Vol.20 No.6

The aim of this study was to investigate the behavior of space usage of Westerners' housing in Korea. For this study, survey and depth-interview method were used for data collection. The questionnaires were distributed to 190 Westerners who currently reside in Seoul and Busan of Korea in October 2002 and March 2003. The number of respondents analyzed in the study was 76 in total. The depth-interview was conducted on 11(15%) of the respondents. The main items of the questionnaire asked about their behaviors and demand of the entrance, bathroom and laundry on housing in Korea. The major findings were as follows: 1) Most of the respondents took off their shoes at the entrance and store shoes in the shoe cabinet. Westerners followed the custom of taking off shoes inside the Korean houses. They appraised the good characteristics of Korean houses were the entrance and shoe closet. 2) In the bathroom, Westerners requested enough storage space and bathroom fixtures that meet their body size. Accordingly, these request should be considered. 3) The Westerners wanted clothing related work to be done in the laundry; therefore, this area should be large enough to place a washer, dryer, and ironing board. Also, our results can contribute to future study on the improvement of Korean houses and point to the good characteristics of Korean houses that may be pass down to the next generation.

한·일 기거양식의 변천비교 : 삼국시대부터 1990년대까지

장상옥,신경주 漢陽大學校 韓國生活科學硏究所 2003 韓國 生活 科學 硏究 Vol.- No.21

The purpose of this study was to compare and predict the seating styles between Korea and Japan from a historical view. For this study, documentary research methods were used. The major findings of this study were as follows: There are similarities and differences points in the seating style in Korea and Japan. The similarities the changing point of the seating style in both countries was the influence of the Western culture from the late 1800s. At that time, the seating style of the upper class was more orientated towards the chair-seating style, while the lower class was more floor-orientated. It took a long time to change the seating style in both countries from floor-seating to chair-seating. There was a cultural conflict with the chair-seating style regarding the housing floor plan and furniture. This means even though housing designs have changed more toward the "Western design," some traditional elements, like a floor-seating style still remain in both Korea and Japan. The current orientation of seating style is toward the chair-seating style. The differences; the period of the change of the seating style was different in both countries. Korea has changed their seating style slowly, but Japan has changed faster than Korea. It depended on the encouragement of the Government, the period of economic growth, floor materials and posture of the body in seating style. The chair-seating style has been populated widely spread in Korea from the 1970s, while in Japan from the mid 1950s. Nowadays, in Japan some of them missed their traditional floor-seating style and want to change the seating style because of their small house size. However, in Korea this phenomenon is not occurring.

고상추출카트리지를 이용한 잔류농약 다중분석과정에서 정제용매에 따른 회수율 비교

두옥주,정보경,김경식,정의근,김진곤,김명희 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

채소류 중 비교적 matrix효과가 적고, 수분 함량이 적절하여 회수율이 양호한 오이를 대상으로 하여 총 16종의 농약 성분을 A, B 두 group으로 나누어, 농약의 동시 다성분 분석의 전처리 과정 중 정제에 사용하는 용출용매로서 5%~20%의 acetone/hexane과 5%~20%의 ethyl acetate/hexane을 사용하여 각각의 회수율을 분석하여 얻은 결과는 다음과 같다. Acetone/hexane을 사용한 경우 5% acetone에서 총 16종의 농약 성분 중 ethalfluralin, trifluralin, chlorothalonil 등 11종의 농약은 높은 회수율을 보였으나, alachlor와 iprodione, endosulfan sulfate, bifenthrin, fenarimol은 매우 낮은 회수율을 나타내었고, 10% 이상의 acetone/hexane에서는 bifenthrin과 fenarimol 제외한 14종의 농약에서 높은 회수율을 얻을수 있었다. 그러나 bifenthrin은 20%의 acetone/hexane을 사용한 경우는 용출되지 않았고, fenarimol은 10% 이하의 acetone/hexane을 사용하였을 때는 낮은 회수율을 나타냈었다. Ethyl acetone/hexane을 사용하여 총 16 종의 농약 성분의 회수율 분석을 한 결과, 5% ethyl acetone에서 ethalfluralin, α-endosulfan, β-endosulfan (A group), vinclozolin, tetradifon, cyprethrin, fenvalerate (B group)에서만 70% 이상의 회수율을 얻었다. 10%의ethyl acetone/hexane을 사용하여 분석한 경우는 trifuralin, chlorothalonil, α-endosulfan, β-endosulfan, endosulfan sulfate(A group), vinclozolin, procymidone, tetradifon, cypermethrin, 그리고 fenvalerate(B group) 에서는 70% 이상의 회수율을 얻었고, 20%의 cthyl acetone/hexane 을 사용하여 분석한 경우는 5%나 10%의 ethyl acetone/hexan 을 사용하여 분석했을 때보다 좋은 결과를 나타내어 전체 16종의 농약 중 14종에서 75% 이상의 회수율을 얻었으나, alachlor와 bifenthrin은 매우 낮은 회수율을 보여서 ethyl acetate 와 hexane의 혼합용매가 이들 두 농약성분의 분석에는 적합하지 않은 용출 용매임을 알 수 있었다. A study was carried out to optimize the cleanup step using a solid-phase extraction (SPE) cartridge, SupelClean^(TM) Florisil, for multiresidue analysis of 16 pesticides in cucumber matrix. Eluting efficiencies of two solvent systems including acetone/n-hexane and ethyl acetate/n-hexane mixtures were critically evaluated by recoveries of target anaytes from the SPE Florisil column. Based on the recovery as a measure of eluting efficiency, an acetone/n-hexane (20/80,v/v) mixture provided more than 80% recovery for 15 pesticides except bifenthrin. In case of ethyl acetate/n-hexane, 14 pesticides showed recoveries higher than 75%, while those for alachior and bifenthrin were less than 30%.

환율변동과 기업가치

이경주,김진옥 濟州大學校 地域社會發展硏究所 1997 논문집 Vol.34 No.1