Carbofuran 의 담수토양중 분해에 대한 토양의 별균과 황산염 첨가의 영향에 관한 연구

두옥주 한국환경농학회 1998 한국환경농학회지 Vol.17 No.1

이 연구는 담수토양 조건에서 토양에 처리된 carbofuran이 시간의 경과에 따라 분해될 때 토양에 존재하는 미생물적 요인이 carbofuran의 분해에 기여하는 정도를 분석하고, 토양내 유기탄소의 양이 비교적 풍부할 때 토양에 존재하는 황산염 성분이 carbofuran의 분해 속도에 미치는 영향을 평가하기 위한 것으로서, 1996년 3월 하순 경기도 시흥시의 논에서 채취한 토양을 대조토양으로 하여 이 대조토양에 cellulose와 황산염의 첨가와 멸균 처리를 하여 대조토양, 멸균토양, 10%의 cellulose 첨가 토양, 10% cellulose와 1%의 황산제일철(FeSO₄·7H₂O) 동시 첨가 토양, 그리고 10% cellulose와 1%의 황산마그네슘(MgSO₄·7H₂O) 동시 첨가토양 등의 다섯 종류의 토양시료를 만들었다. 이들 토양시료에 총 농도 50ppm가 되도록 carbofuran을 첨가하여 25℃에서 4주간 배양하면서 carbofuran의 토양내 잔류량을 측정하였다. 멸균토양의 carbofuran의 잔류량은 대조토양에 비해 매우 많았는데(p$lt;0.05), 4주 동안 배양시 대조토양에 비해 잔류량이 약 18% 정도 많았다. 토양내 carbofuran의 잔류량을 배양 4주 동안 측정한 결과, 10%의 cellulose와 황산마그네슘을 동시에 첨가한 토양에서 carbofuran의 잔류량이 대조토양과 10% cellulose만을 첨가한 토양이나 10% cellulose와 황산제일철 동시 첨가 토양에 비해 매우 많아서(p$lt;0.01), 황산염과 마그네슘 함량이 일정 농도 이상으로 높은 토양에서 carbofuran의 분해가 지연되는 결과를 나타내었다. Carbofuran was incubated for four weeks in five types of paddy soil samples at 25℃. The soil samples prepared in the study were as follows : control soil, sterilized soil, 10% cellulose added soil, 10% cellulose and 1% ferrous sulfate added soil, and 10% cellulose and 1% magnesium sulfate added soil. The degradation rate of carbofuran was significantly decreased(p$lt;0.05) in sterilized soil. The degradation rate of carbofuran was significantly decreased by addition of cellulose(p$lt;0.05) in ferrous sulfate added soil and magnesium sulfate added soil(p$lt;0.01).

大都市 低所得層 幼兒의 營養에 關한 考察 : Based on the Data of Nutivition Survey through 1977-1986 1977-86年 10年間의 營養實態 調査를 中心으로

杜玉珠 서울大學校保健大學院 1994 國民保健硏究所硏究論叢 Vol.4 No.2

This study have reviewed the nutritional status of pre-school children of the low income area in Seoul, based on the survey data from 1977 to 1987. The nutritional status of children was assessed by anthropometric method and diet method. The results of the study were summarized as follow: 1. About 47 percentage of pre-school children of the low income area in Seoul corresponded to mild malnutritional stage assessed by anthropometric method in 1977. It decreased to 4% in 1983. 2. Assessed by diet method, their total daily calori intakes were in normal range compared to recommended dietary allowance. But their daily intake amounts of protein, calcium, iron and vitamins(except thiamin) represented defficient compared with recommended dietary allowance and those of children of other area in Seoul. These results implicate that their inferior physical development condition was caused by their poor nutritional status. Therefore, nutritional education programs should be planned and performed for their parents and the quality and extent of day care lunch such area should be improved.

서울시 일부 제과점 제품의 지방산 조성 및 트랜스지방 함량 분석

두옥주,김애경,김미선,박영혜,박혜원,김연천,채영주 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.2

The composition of fatty acids in bakery products from 35 bakeries in Seoul was analyzed by GCFID. The contents of crude fat in bakery products were represented 9.54-44.30% in pastries, 14.67-41.22% in cookies and 7.63-28.15% in whipped cream cakes. The total saturated fatty acid content in pastries, cookies and whipped cream cakes were 12.8-75.1%, 8.7-81.6% and 50.2-85.9% respectively. The proportion of trans fatty acids in pastries ranged from 0.00 to 3.37%. Cookies and whipped cream cakes were represented varying amount of trans fatty acid with highest value of 4.55% in cookies and 2.13% in whipped cream cakes. Total estimated daily intakes of fatty acids from pastries and whipped cream cakes were as follows : 1.2 g/day/person for saturated fatty acids and 0.014 g/day/person for trans fatty acids.

저층(Sediment)에서 분리된 미생물에 의한 Carbaryl (1-naphthyl-N-methylcarbamate)의 혐기적 분해에 관한 연구

두옥주,정문호 한국환경보건학회 1990 한국환경보건학회지 Vol.16 No.2

Carbaryl was degradaded to monomethylamine(MA) by bacterium. which isolated from lake sediment. Its carbaryl degradation was maximized when grown on mineral salt medium conditioning 20 $\mu$M of carbaryl as a sole carbon source at 26${\circ}$C and initial pH 7.0-9.0 Its degradation ability was minimized at initial pH 3.0 and 5.0 The percent conversions { (moles of MA in excess of control / mole of carbaryl added) $\times$ 100} for 10. 20. 40 and 80 $\mu$M of carbaryl were 18.6. 16.1. 18.1 and 11.6 respectively. It suggests that increasing amount of carbaryl added above 80 $\mu$M. the percent conversion should be decreased. The MA production by the bacterium was lineary related to the cultural time. After 7days culture. its percent conversion was 46.2. and this result suggests that a half amount of carbaryl would be hydrolyzed to MA by the bacterium within 8 days.

고상추출카트리지를 이용한 다중 잔류농약분석 시 정제용매에 따른 회수율 비교

두옥주,정보경,김경식,정의근,김진곤,김명희 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

고상추출카트리지를 이용한 잔류농약 다중분석과정에서 정제용매에 따른 회수율 비교

두옥주,정보경,김경식,정의근,김진곤,김명희 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

채소류 중 비교적 matrix효과가 적고, 수분 함량이 적절하여 회수율이 양호한 오이를 대상으로 하여 총 16종의 농약 성분을 A, B 두 group으로 나누어, 농약의 동시 다성분 분석의 전처리 과정 중 정제에 사용하는 용출용매로서 5%~20%의 acetone/hexane과 5%~20%의 ethyl acetate/hexane을 사용하여 각각의 회수율을 분석하여 얻은 결과는 다음과 같다. Acetone/hexane을 사용한 경우 5% acetone에서 총 16종의 농약 성분 중 ethalfluralin, trifluralin, chlorothalonil 등 11종의 농약은 높은 회수율을 보였으나, alachlor와 iprodione, endosulfan sulfate, bifenthrin, fenarimol은 매우 낮은 회수율을 나타내었고, 10% 이상의 acetone/hexane에서는 bifenthrin과 fenarimol 제외한 14종의 농약에서 높은 회수율을 얻을수 있었다. 그러나 bifenthrin은 20%의 acetone/hexane을 사용한 경우는 용출되지 않았고, fenarimol은 10% 이하의 acetone/hexane을 사용하였을 때는 낮은 회수율을 나타냈었다. Ethyl acetone/hexane을 사용하여 총 16 종의 농약 성분의 회수율 분석을 한 결과, 5% ethyl acetone에서 ethalfluralin, α-endosulfan, β-endosulfan (A group), vinclozolin, tetradifon, cyprethrin, fenvalerate (B group)에서만 70% 이상의 회수율을 얻었다. 10%의ethyl acetone/hexane을 사용하여 분석한 경우는 trifuralin, chlorothalonil, α-endosulfan, β-endosulfan, endosulfan sulfate(A group), vinclozolin, procymidone, tetradifon, cypermethrin, 그리고 fenvalerate(B group) 에서는 70% 이상의 회수율을 얻었고, 20%의 cthyl acetone/hexane 을 사용하여 분석한 경우는 5%나 10%의 ethyl acetone/hexan 을 사용하여 분석했을 때보다 좋은 결과를 나타내어 전체 16종의 농약 중 14종에서 75% 이상의 회수율을 얻었으나, alachlor와 bifenthrin은 매우 낮은 회수율을 보여서 ethyl acetate 와 hexane의 혼합용매가 이들 두 농약성분의 분석에는 적합하지 않은 용출 용매임을 알 수 있었다. A study was carried out to optimize the cleanup step using a solid-phase extraction (SPE) cartridge, SupelClean^(TM) Florisil, for multiresidue analysis of 16 pesticides in cucumber matrix. Eluting efficiencies of two solvent systems including acetone/n-hexane and ethyl acetate/n-hexane mixtures were critically evaluated by recoveries of target anaytes from the SPE Florisil column. Based on the recovery as a measure of eluting efficiency, an acetone/n-hexane (20/80,v/v) mixture provided more than 80% recovery for 15 pesticides except bifenthrin. In case of ethyl acetate/n-hexane, 14 pesticides showed recoveries higher than 75%, while those for alachior and bifenthrin were less than 30%.

底i尼(Sediment}에서 分離된 微生物에 依한 Carbaryl (l-naphthyl-N -methylcarbamate) 의 購氣的 分解에 關한 硏究

두옥주(Ock Joo Du),정문호(Moon Ho Chug) 한국환경보건학회 1990 한국환경보건학회지 Vol.16 No.2

Carbaryl was degradaded to monomethylamine(MA) by bacterium. which isolated from lake sedi. ment. Its carbaryl degradation was maximized when grown on mineral salt medium conditioning 20 þ.M of carbaryl as a sole carbon source at 26t and initial pH ï.0-9.0 Its degradation ability was minimized at initial pH 3.0 and 5.0 The percent cαlversions I (moles of MA in excess of control / mole of carbaryl added) X 100 I for 10‘ 20.40 and 80 μM of carbaryl were 18.6. 16. 1. 18.1 and 1l.6 respectively. It :5uggests that increasing amount of carbaryl added above 80 Þ. M. the percent conversion should be decreased. The MA production by the bacterium was lineary related to the cultural time‘After 7days culture. its percent conversion was 46.2‘ and this result suggests that a half amount of carbaryl would be hydrolyzed to MA by the bacterium within 8 davs

서울지역 제과·제빵점에서 판매되는 빵류와 과자류의 나트륨 및 당 함량 조사

김미선,두옥주,박영혜,박혜원,금진영,김연천,채영주 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.1

The objective of this study was to investigate the extent of total sugar and sodium in 129 different kinds of bakery products, breads and cookies, sold at bakeshops in Seoul metropolitan area. The bakery products produced by bakeshops on a small scale were not applied by clauses of mandatory nutrition label for children's taste food. All types of free sugars -fructose, glucose, sucrose, lactose and maltose- were detected in breads, but only fructose and sucrose were detected in cookies. The average amount of sucrose per 100 g of breads was 6.24 g, of cookies was 30.03 g. Breads and cookies amounting to 100 g of sample contained total sugar of 11.19 g and 30.38 g, respectively. The average amounts of sodium in breads and cookies were 120.71 mg/100 g, 70.76 mg/100 g, respectively. When the contents of total sugar in breads and cookies per one serving size were compared to WHO guidelines, the percentages were 15.7% and 18.2% about recommended daily intake of total sugar of 50 g. When it come to sodium, the bakery products had range of 1.1~6.5% to 2000 mg of daily intake of sodium recommended by WHO.

HPLC-MS/MS를 이용한 어린이 기호식품중의 감미료 분석

김일영,두옥주,이성득,박영혜,김미선,배청호,채영주 한국식품위생안전성학회 2010 한국식품위생안전성학회지 Vol.25 No.2

A HPLC-MS/MS method was developed for simultaneous determination of six sweeteners (acesulfame-K, cyclamate, saccharin, sucralose, stevioside, aspartame) in children's favorite foods. The procedure involves an extraction of the six sweeteners with 50% methanol solution, sample clean-up using the Carrez clearing reagent and filtering with cartridge filter. The HPLC separation was performed on a Hypersil Gold (150 mm × 2.1 mm 5 um) column using the water/acetonitrile mobile phase (95:5). Mass spectrometric analysis was carried out using the TSQ Quantum Ultra operated in negative and positive ESI/SRM. With this method, good linear relationship, sensitivity and reproducibility were obtained. The spike recoveries of six sweeteners for 2 kinds of foods spiked into 0.4 mg/kg ranged from 87.4 to 114.7%. The detection limits were above 0.02 mg/kg. The method has been applied to determination of six sweeteners in children's favorite foods.

Strontium 90의 골수 발암성에 대한 전계장의 영향에 관한 실험적 연구

정문호,두옥주 한국환경보건학회 1994 한국환경보건학회지 Vol.20 No.3

Sprague Dawley rats were exposed to electric fields (6,000 V, 10 kV/m, 30 min/day, 6 days per week) and injected strontium 90 (681 kbq/rat, one time) through abdominal cavity (strontium 90 and electric field complexed exposure group). In parallel, series with the electric field exposure only, strontium 90 injection only and control groups were run. Every group was consisted of 110 rats (55 male and 55 female). This animal experiment was performed from May to December in 1993. This results were conducted to investigate the effect of electric field for 11 weeks. The results are summarized as follows: 1. Ornithine decarboxylase (ODC) activity in rat's bone marrow cells: The ODC values was significantly increased in Sr$^{90}$ injection group and Sr$^{90}$ and electric field complexed exposure group as compared with that of control group (p<0.05). The ODC value was significantly decreased in electric field and Sr$^{90}$ complexed exposure group in comparison with Sr$^{90}$ injection group (p<0.05). The ODC values of electric field only exposure group was not different to that of control group (p>0.05). 2. The amount of Sr$^{90}$ accumulation in the femur, kidney and spleen:The accumulation amount of Sr$^{90}$ in the femur of Sr$^{90}$ injection group represented higher value than that of electric field and Sr$^{90}$ complexed exposure group (p<0.05). In the kidney and spleen, the difference between electric field and Sr$^{90}$ complexed exposure group and Sr$^{90}$ injection group wasn't observed. 3. The counts of white cells in blood of Sr9?injection group was decreased as compared with the value from control group and electric field and Sr$^{90}$ complexed exposure group (p<0.05). The rat's body weight, red blood cell counts and the weight data of liver, kidney and spleen did not show differences among four groups.