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Zhao Linjie,Yang Mao,Xiao Chengjian,Gong Yu,Ran Guangming,Chen Xiaojun,Li Jiamao,Yue Lei,Chen Chao,Hou Jingwei,Wang Heyi,Long Xinggui,Peng Shuming 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1
Understanding the tritium release and retention behavior of candidate tritium breeder materials is crucial for breeder blanket design. Recently, a melt spraying process was developed to prepare Li4SiO4 pebbles, which were subsequently subjected to the in-pile tritium production and extraction platform in China Mianyang Research Reactor (CMRR) to investigate their in-situ tritium release behavior and irradiation performance. The results demonstrate that HT is the main tritium release form, and adding hydrogen to the purge gas reduces tritium retention while increasing the HT percent in the purge gas. Post-irradiation experiments reveal that the irradiated pebbles darken in color and their grains swell, but the mechanical properties remain largely unchanged. It is concluded that the tritium residence time of Li4SiO4 made by melt spraying method at 467 ◦C is approximately 23.34 h. High-density Li4SiO4 pebbles exhibit tritium release at relatively low temperatures (<600 ◦C) that is mainly controlled by bulk diffusion. The diffusion coefficient at 525 ◦C and 550 ◦C is 1.19 × 10 11 cm2/s and 5.34 × 10 11 cm2/s, respectively, with corresponding tritium residence times of 21.3 hours and 4.7 hours.
Fang Gong,Tong Zhang,Jun Sheng,Jing Li,Xianghong Wang,Zhenming Chi 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5
The extracellular inulinase of the marine yeast Pichia guilliermondii strain 1 was purified to homogeneity resulting in a 7.2-fold increase in specific inulinase activity. The molecular mass of the purified enzyme was estimated to be 50.0 kDa. The op-timal pH and temperature for the purified enzyme were 6.0 and 60C, respectively. The enzyme was activated by Mn²+, Ca²+, K+, Li+, Na+, Fe³+, Fe²+, Cu²+, and Co²+, but Mg²+, Hg²+, and Ag+ inhibited activity. The enzyme was strongly inhibited by phenylmethanesulphonyl fluoride (PMSF), iodoacetic acid, EDTA, and 1, 10-phenanthroline. The Km and Vmax values of the purified inulinase for inulin were 21.1 mg/mL and 0.08 mg/min, respectively. A large number of monosaccharides were de-tected after the hydrolysis of inulin. The deduced protein sequence from the cloned P. guilliermondii strain 1 inulinase gene contained the consensus motifs R-D-P-K-V-F-W-H and W-M-N-D-P-N-G, which are conserved among the inulinases from other microorganisms.
Qin, Xiu-Yuan,Lee, Jinhyuk,Zheng, Li,Yang, Jun-Mo,Gong, Yan,Park, Yong-Doo Elsevier 2018 Process biochemistry Vol.65 No.-
<P><B>Abstract</B></P> <P>The phenomenon of α-glucosidase inhibition has attracted the attention of researchers due to its association with type 2 diabetes treatment in humans. In this study, we found that 2-thiobarbituric acid (TBA) induces complex inhibition of α-glucosidase using kinetics tests and molecular dynamics (MD) simulations. Computational MD and docking simulations demonstrate that TBA interacts with three residues on active sites of α-glucosidase such as Met69, Arg212, and His348. These biochemical tests indicate that TBA reversibly inhibits α-glucosidase in a parabolic noncompetitive manner (<I>IC</I> <SUB>50</SUB> =17.13±1.14mM; <I>K</I> <SUB>i</SUB> =13.25±0.56mM) and that this inhibition is accompanied by a biphasic kinetic process. The tertiary conformational changes were not synchronized with TBA inhibition but we observed hydrophobic disruption after inactivation at higher concentrations of TBA. Our results provide insight into the functional roles of residues located at the active sites of α-glucosidase, and we suggest that compounds similar to TBA (heterocyclic compounds) targeting the key residues of active sites are potential α-glucosidase inhibitors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 2-Thiobarbituric acid (TBA) induces complex inhibition of α-glucosidase. </LI> <LI> Computational MD simulations demonstrate that TBA interacts with Met69, Arg212, and His348. </LI> <LI> TBA reversibly inhibits α-glucosidase in a parabolic noncompetitive manner (<I>IC</I> <SUB>50</SUB> =17.13±1.14mM; <I>K</I> <SUB>i</SUB> =13.25±0.56mM). </LI> <LI> The high dose of TBA induces hydrophobic disruption after inactivation. </LI> <LI> Heterocyclic compounds targeting the key residues of active sites are potential α-glucosidase inhibitors. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Pyrophosphate-triggered nanoaggregates with aggregation-induced emission
Li, Chun-Tao,Xu, You-Liang,Yang, Jian-Gong,Chen, Yong,Kim, Hyeong Seok,Cao, Qian-Yong,Kim, Jong Seung Elsevier Sequoia 2017 Sensors and actuators. B Chemical Vol.251 No.-
<P><B>Abstract</B></P> <P>A novel tetraphenylethene-based probe bearing bis-imidazolium anion donors is herein reported for pyrophosphate anion recognition. This probe can self-assemble finite, small sphere nanoaggregates with very weak emission in aqueous solution, and changes into large rod-like nanoaggregates with strong aggregation-induced emission upon binding with the pyrophosphate anion.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A bis-imidazolium functionalized tetraphenylethene probe was prepared. </LI> <LI> This probe self-assemble finite small sphere nanoaggregates in aqueous solution. </LI> <LI> The probe can recognize pyrophosphate anion with strong aggregation-induced emission. </LI> <LI> The probe/pyrophosphate assembly can fluorescence assay alkaline phosphatase. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A novel nanoaggregates for recognition of pyrophosphate anion with aggregation-induced emission in pure aqueous solution is introduced.</P> <P>[DISPLAY OMISSION]</P>
Gong-Chen Zhang,Wen-Li Zhu,Jun-Yi Gai,Yue-Lin Zhu,Li-Fei Yang 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.1
Vegetable soybeans [Glycine max (L.) Merrill] are susceptible to salt stress and, thus, soil salinity can severely affect their growth and productivity. To enhance the salt tolerance of vegetable soybeans, a novel Solanum torvum Swartz Δ1-pyrroline-5-carboxylate synthetase gene (StP5CS, GenBank accession number: JN606861) that encodes a critical regulatory enzyme in proline biosynthesis was transformed into the cultivar NY-1001 using an Agrobacteriummediated transformation method. PCR and Southern blot analyses indicated that two independent T0 fertile transgenic plants were generated. The transgenic plants transmitted the transgenes into their T1 progeny in a 3:1 ratio. The T2 and T3 homozygous transgenic lines (HTLs) were examined for salt tolerance in pot and hydroponic cultures, respectively. The StP5CS overexpression conferred salt tolerance in T2 and T3 HTLs. Under NaCl stress conditions, the leaf scorch scores of T2 and T3 HTLs were significantly lower than those of wild-type (WT) plants. The plant height, leaf area, relative chlorophyll content, and number of fresh pods of T2 and T3 HTLs were significantly higher than those of WT plants. Compared with WT plants, T2 and T3 HTLs had significantly higher levels of proline and significantly lower levels of membrane lipid peroxidation. These results indicate that StP5CS overexpression in HTLs results in enhanced salt tolerance associated with higher levels of proline accumulation under salinity stress and that StP5CS can be utilized to improve salinity tolerance in vegetable crop genetic engineering.
The Genetic Diversity of Seven Pig Breeds in China, Estimated by Means of Microsatellites
Li, X.,Li, K.,Fan, B.,Gong, Y.,Zhao, S.,Peng, Z.,Liu, B. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.9
The polymorphisms of six microsatellites were investigated in four indigenous pig breeds (Erhualian, Tongcheng, Qingping and Wannanhua) and three introduced breeds (Large White, Landrace and Duroc) in China, and the genetic variations within and among populations were analyzed. The results showed that genetic diversity of Chinese indigenous pig breeds is higher than that of the introduced pig breeds. The clustering of seven breeds is consistent with their geographical distribution approximately. Estimated time of breed divergence ranged from 653 to 1856 years.
Li, Xiao-Dong,Sabiu, Cristiano G.,Park, Changbom,Wang, Yuting,Zhao, Gong-bo,Park, Hyunbae,Shafieloo, Arman,Kim, Juhan,Hong, Sungwook E. American Astronomical Society 2018 The Astrophysical journal Vol.856 No.2
<P>We perform an anisotropic clustering analysis of 1,133,326 galaxies from the Sloan Digital Sky Survey (SDSS-III) Baryon Oscillation Spectroscopic Survey Data Release 12 covering the redshift range 0.15 < z < 0.69. The geometrical distortions of the galaxy positions, caused by incorrect assumptions in the cosmological model, are captured in the anisotropic two-point correlation function on scales of 6-40 h(-1) Mpc. The redshift evolution of this anisotropic clustering is used to place constraints on the cosmological parameters. We improve the methodology of Li et al. to enable efficient exploration of high-dimensional cosmological parameter spaces, and apply it to the Chevallier-Polarski-Linder parameterization of dark energy, w = w(0) + w(a)z/(1 + z). In combination with data on the cosmic microwave background, baryon acoustic oscillations, Type Ia supernovae, and H-0 from Cepheids, we obtain Omega(m) = 0.301 +/- 0.008, w(0) = -1.042 +/- 0.067, and w(a) = -0.07 +/- 0.29 (68.3% CL). Adding our new Alcock-Paczynski measurements to the aforementioned results reduces the error bars by similar to 30%-40% and improves the dark-energy figure of merit by a factor of similar to 2. We check the robustness of the results using realistic mock galaxy catalogs.</P>
An integrated porous Ni3S2 electrode assisted by copper with advanced performance for sodium storage
Li-Feng Zhou,He Gong,Yisong Wang,He Jia,Li-Ying Liu,Tao Du 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-
Sodium ion battery has been rapidly consolidating its status of the dominant power supplier for largescaleenergy storage systems as a candidate of lithium ion battery and has gotten widely attention. In thiswork, an integrated porous Ni3S2 electrode assisted by copper using for sodium storage showed anadvanced electrochemical performance and long life-span. Coupled with Cu particles planted on the surfaceof Ni foam, electron transfer can be fast induced, thus avoiding the electrode damage due to collectionelectron. It further achieved a superior retention of 88.7 % with the capacity of 417 mAh g1. In thekinetics analysis, the capacitance-controlled capacity in the electrochemical process dominated, and thefast calculated Na+ diffusion coefficients was 4 10-10 cm2 s1. Therefore, it deserves attention and furtherresearch for the commercial application of large-scale energy storage.
Li, Qian,Yang, Mengmeng,Gong, Cheng,Chopdekar, Rajesh V.,N’Diaye, Alpha T.,Turner, John,Chen, Gong,Scholl, Andreas,Shafer, Padraic,Arenholz, Elke,Schmid, Andreas K.,Wang, Sheng,Liu, Kai,Gao, Nan,Admas American Chemical Society 2018 NANO LETTERS Vol.18 No.9
<P>Magnetic van der Waals (vdW) materials have emerged as promising candidates for spintronics applications, especially after the recent discovery of intrinsic ferromagnetism in monolayer vdW materials. There has been a critical need for tunable ferromagnetic vdW materials beyond room temperature. Here, we report a real-space imaging study of itinerant ferromagnet Fe<SUB>3</SUB>GeTe<SUB>2</SUB> and the enhancement of its Curie temperature well above ambient temperature. We find that the magnetic long-range order in Fe<SUB>3</SUB>GeTe<SUB>2</SUB> is characterized by an unconventional out-of-plane stripe-domain phase. In Fe<SUB>3</SUB>GeTe<SUB>2</SUB> microstructures patterned by a focused ion beam, the out-of-plane stripe domain phase undergoes a surprising transition at 230 K to an in-plane vortex phase that persists beyond room temperature. The discovery of tunable ferromagnetism in Fe<SUB>3</SUB>GeTe<SUB>2</SUB> materials opens up vast opportunities for utilizing vdW magnets in room-temperature spintronics devices.</P> [FIG OMISSION]</BR>