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( Hyeok-won Lee ),( Hee-suk Lee ),( Chun-suk Kim ),( Jin-gyeom Lee ),( Won-kyo Kim ),( Eun-gyo Lee ),( Hong-weon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.2
Controlling the residual glucose concentration is important for improving productivity in L-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio (R<sub>C/N</sub>) of glucose and ammonia water was predetermined via a stoichiometric approach, on the basis of glucose-ammonia water consumption rates. In a 5-L fermenter, 102 g/l L-threonine was obtained using our glucose-ammonia water combined feeding strategy, which was then successfully applied in a 500-L fermenter (89 g/l). Therefore, we conclude that an automatic combination feeding strategy is suitable for improving L-threonine production.
Production of Bio-Based Isoprene by the Mevalonate Pathway Cassette in Ralstonia eutropha
( Hyeok-won Lee ),( Jung-ho Park ),( Hee-seok Lee ),( Wonho Choi ),( Sung-hwa Seo ),( Irika Devi Anggraini ),( Eui-sung Choi ),( Hong-weon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.10
Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. Ralstonia eutropha can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of R. eutropha H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter (1.9 ± 0.24 μg/l) than when using the lac promoter (1.5 ± 0.2 μg/l). Additionally, the use of three J5 promoters was more efficient (3.8 ± 0.18 μg/l) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that R. eutropha can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.
최원영 ( Weon Young Choi ),정재혁 ( Jae Hyeok Jeong ),김선 ( Sun Kim ) 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.3
This study was conducted to identify the optimal nitrogen amount of rice transplanting cultivation in newly reclaimed land, in light of minimum salt damage and high yield of rice. The nitrogen amount treatments were 11 kg/10 a, 14 kg/10 a, 17 kg/10 a, 20 kg/10 a and diagnostic fertilization. The average of soil salinity fell from 0.06% to 0.03% during 2 years after flooding and rice transplantation. The heading date was August 16 nitrogen at rate of 11 kg/10 a and 14 kg/10 a, August 17 at 17 kg/10 a and 20 kg/10 a. The lodging index tended to rise in proportion to nitrogen application amount; any of rice plants were not lodged at 11 kg/10 a, but the lodging index was 1 and 3 at 14 kg/10 a and 17 kg/10 a respectively. At 20 kg/10 a, however, over half of rice plants were lodged with the lodging index 7. The panicle number per hill was the highest at 17 kg/10 a, followed by the diagnostic fertilization, 20 kg/10 a, 14 kg/10 a and 11 kg/10 a. The percentage of ripened grains was similar to the weight of 1,000 grains of brown rice, and thus the rice yield was the highest at the diagnostic fertilization and at 17 kg/10 a equally, followed by 14 kg/10 a, 20 kg/10 a and 11 kg/10 a. The percentage of perfect kernels was the highest at 14 kg/10 a, followed by the diagnostic fertilization and 11 kg/10 a, 17 kg/10 a, and 20 kg/10 a. The amylose content was not significantly different by nitrogen application amount, but the protein content was closely correlated with it. Altogether, the optimal nitrogen application amount was found to be 16 to 18 kg/10 a on the premise that rice seedlings would be mechanically transplanted in newly reclaimed land.
Jee Hyeok Chung,Sunjin Yim,Il-Sik Cho,Seung-Weon Lim,Il-Hyung Yang,Jeong Hyun Ha,Sukwha Kim,Seung-Hak Baek 대한치과교정학회 2020 대한치과교정학회지 Vol.50 No.6
Objective: To investigate the distribution, side involvement, phenotype, and associated anomalies of Korean patients with craniofacial clefts (CFC). Methods: The samples consisted of 38 CFC patients, who were treated at Seoul National University Dental Hospital during 1998–2018. The Tessier cleft type, sex, side involvement, phenotype, and associated anomalies were investigated using nonparametric statistical analysis. Results: The three most common types were #7 cleft, followed by #0 cleft and #14 cleft. There was no difference between the frequency of male and female. Patients with #0 cleft exhibited nasal deformity, bony defect, and missing teeth in the premaxilla, midline cleft lip, and eye problems. A patient with #3 cleft (unilateral type) exhibited bilateral cleft lip and alveolus. All patients with #4 cleft were the bilateral type, including a combination of #3 and #4 clefts, and had multiple missing teeth. A patient with #5 cleft (unilateral type) had a posterior openbite. In patients with #7 cleft, the unilateral type was more prevalent than the bilateral type (87.0% vs. 13.0%, p < 0.001). Sixteen patients showed hemifacial microsomia (HFM), Goldenhar syndrome, and unilateral cleft lip and palate (UCLP). There was a significant match in the side involvement of #7 cleft and HFM (87.5%, p < 0.01). Patients with #14 cleft had plagiocephaly, UCLP, or hyperterorbitism. A patient with #30 cleft exhibited tongue tie and missing tooth. Conclusions: Due to the diverse associated craniofacial anomalies in patients with CFC, a multidisciplinary approach involving a well-experienced cooperative team is mandatory for these patients.
A Study on Safety and Plans for Standardization of Stage Broadcasting Lighting Fixtures C-TYPE
Jang-Weon Lee(이장원),Hyeok-Hwan Kwon(권혁환) 한국엔터테인먼트산업학회 2015 한국엔터테인먼트산업학회논문지 Vol.9 No.2
무대방송조명기구는 전기사용기기로 전기관련 법규에 준하여 시설해야 한다. 하지만, 현재 조명기구를 활용하는 공간에 KC나 KS인증을 받지 않는 상태에 업체마다의 다른 규격으로 C-TYPE 플러그를 사용하고 있는 실정이다. 그로 인하여 접촉 불량으로 인한 쇼트 및 수명단축 등 전기적 안전성에 대한 문제가 있다. 우리는 업체별로 C-TYPE 플러그의 규격을 실측하였고, 그로 인하여 업체별 조금씩 다른 사이즈 및 구성을 발견할 수 있었다. 크기가 달라서 결속시 작은 공백으로 인한 쇼트의 문제점이 발견할 수 있었으며, 결속 불량으로 인한 수명단축이 발생될 수 있는 소지 또한 확인 할 수 있었다. 그러므로 적극적인 표준화 작업이 이루어 C-TYPE 장치에 대한 전기적 안정성 및 표준화를 통한 제품을 만들기 위해 노력과 추가적인 연구가 필요로 하겠다. Stage Lighting Equipment should be broadcast facility, in accordance with electrical codes into Electricity using equipment. However, Which is used by C-TYPE PLUG, in a space to take advantage of the current luminaire, On the other specifications of each company. By state does not received KC and KS Certification. Thereby are problem with electrical safety in short and life-shortening. etc,, Due to poor contact. We have a Manufacturer, It measured the dimensions of the C-TYPE PLUG. Thereby Could find Manufacturer little different size and configuration. what for small space, Also found when Service life, what for poor cohesion, which may be generated, Therefore Active standardization work is done, I"ll need to try and further research, of make product through electrical stability and standardization for C-TYPE device.
( Sung Hyeok Ryou ),( Jong Wook Bae ),( Hyun Jin Baek ),( Doo Hyuk Lee ),( Sang Won Lee ),( Gyu Ho Choi ),( Kyu Hyung Han ),( Se Weon Kim ),( Hyunbeom Kim ),( Goohyeon Hong ) 대한결핵 및 호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.4
Pulmonary pneumatoceles are air-filled thin-walled spaces within the lung and are rare in adult cases of pneumonia. We report the case of a 74-year-old male who was admitted with a cough and sputum production. He had been treated with oral dexamethasone since a brain tumorectomy 6 months prior. Contrast-enhanced computed tomography (CT) of the chest revealed a large pneumatocele in the right middle lobe and peripheral pneumonic consolidation. Bronchoalveolar lavage was performed; cultures identified extended-spectrum β-lactamase (ESBL) producing Proteus mirabilis. A 4-week course of intravenous ertapenem was administered, and the pneumatocele with pneumonia resolved on follow-up chest CT. To the best of our knowledge, this is the first reported case of pulmonary pneumatocele caused by ESBL-producing P. mirabilis associated with pneumonia.
Lee, Hyeok-Won,Park, Jung-Ho,Lee, Hee-Suk,Kim, Chun-Suk,Lee, Jin-Gyeom,Kim, Won-kyo,Ryu, Kyoung-Hwa,Ahn, Jung-Oh,Lee, Eun-Gyo,Kim, Seon-Won,Jeon, Jong-Min,Yang, Yung-Hun,Choi, Eui-Sung,Lee, Hong-Weon Society for Bioscience and Bioengineering, Japan 2019 Journal of bioscience and bioengineering Vol. No.
<P>Many volatile compounds, such as isoprene, a precursor used in the synthesis of natural rubber, have been produced through fermentation using genetically engineered microorganisms. Despite this biotechnological success, measuring the concentrations of volatile compounds during fermentation is difficult because of their high volatility. In current systems, off-line analytical methods usually lead to product loss, whereas on-line methods raise the production cost due to the requirement of complex devices. Here, we developed a novel on-line gas chromatography (GC)-based system for analyzing the concentration of isoprene with the aim to minimize the cost and requirement for devices as compared to current strategies. In this system, a programmable logic controller is used to combine conventional GC with a syringe pump module (SPM) directly connected to the exhaust pipe of the fermentor, and isoprene-containing samples are continuously pumped from the SPM into the GC using an air cylinder recycle stream. We showed that this novel system enables isoprene analysis during fermentation with convenient equipment and without the requirement of an expensive desorption tube. Furthermore, this system may be extended to the detection of other volatile organic compounds in fermentation or chemical processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This system analyzes the concentration of isoprene in aerobic fermentation. </LI> <LI> PLC is used to combine conventional GC with SPM directly connected to fermenter. </LI> <LI> Produced isoprene is continuously analyzed using the GC. </LI> <LI> This system may be extended to the detection of other volatile organic compounds. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>