우리나라에서 솔껍질깍지벌레의 분포확산

임유진,이상명,김동수,김준범,최광식,이신혜,박정규,이동운,Lim, Eu-Gene,Lee, Sang-Myeong,Kim, Dong-Soo,Kim, Joon-Bum,Lee, Shin-Hye,Choi, Kwang-Sik,Park, Chung-Gyoo,Lee, Dong-Woon 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.1

솔껍질깍지벌레는 우리나라 곰솔림의 주요한 해충의 하나이다. 본 연구는 우리나라에서 솔껍질깍지벌레의 분포를 알아보기 위하여 7개도 3개 광역시, 91개 시군구, 686 읍면동의 해송림에서 2010년 밀도를 조사하였다. 5개도 2개 광역시에서 솔껍질깍지벌레가 서식하고 있었는데 시군구별로는 조사대상의 64.8%인 59개 시군구에서 서식이 확인되었다. 솔껍질깍지벌레의 분포는 남해안 전역과 동해안의 포항, 서해안의 충남 보령지역까지 분포하였다. 충북과 대전광역시 및 제주도에서는 솔껍질깍지벌레의 분포가 확인되지 않았다. 부산지역은 16곳의 모든 구에서 솔껍질깍지벌레가 분포하였고, 시군 단위에서는 전남 해남군의 솔껍질깍지벌레 평균밀도가 1.713마리/0.785 $cm^2$로 가장 높았으며 읍면동 단위에서는 해남군 송지면이 6.36마리/0.785 $cm^2$로 가장 높았다. 곰솔 가지 내의 솔껍질깍지벌레 밀도와 솔껍질깍지벌레가 서식하고 있는 빈도와는 높은 상관관계가 있었다(상관계수=0.89). The black pine bast scale, $Masucoccus$ $thunbergianae$ (Hemiptera: Margarodidae), is a serious pest of the Japanese black pine, $Pinus$ $thunbergii$, in Korea. The distribution of the black pine bast scale was examined, looking overall at 686 towns (eup), townships (myeon) or neighborhoods (dong). There were Japanese black pine ($Pinus$ $thunbergii$) forests in 91 cities, counties (gun) and borough (gu), in seven provinces and three metropolitan cities during 2010. Black pine bast scale were found in 64.8% of cities or counties or borough (59) in 7 provinces and 3 metropolitan cities, and were distributed in all South Costal regions, Pohang in East Costal region and Boryeong in West Costal region. Chungcheongbukdo, Daejeon and Jeju did not have black pine bast scale. All the gu regions in Busan had black pine bast scale, of which the area with the highest prevalence was Haenam in Jeollanamdo (1.713 crawlers/0.785 $cm^2$). Songji-myeon had the highest occurrence rate (6.36 crawlers/0.785 $cm^2$) from the towns, township and dong. The density of black pine bast scale in twigs was highly correlated with percentage of the sample with scale (Correlation coefficacy=0.89).

염기서열특이 - 중합효소연쇄반응법을 이용한 Rh C/c, E/e 혈액형항원 유전자형 검사

서동희,오흥범,황유성,김상인 大韓輸血學會 1999 大韓輸血學會誌 Vol.10 No.1

外國籍企業의 會計報告制度에 관한 硏究

朴範鎬,申東洙,蔡奎學,李均,金喆中 弘益大學校 1983 弘大論叢 Vol.15 No.1

In Accounting, a new problematic field has been recognized with the development and changes in industrialized society. An accounting system of a multinational corporation emerged from different accounting environments between one country and another. This environment can be summarized as follows; (1) Accounting measurement that affect financial position and management performance of an accounting entity are made by foreign currency, not by domestic currency or reporting currency of a parent company. (2) The exchange rate is not fixed, but fluctuating. (3) In case the foreign business activity is conducted by the foreign entity, separated from the parent company at home, the enterprise activity is done under entirely different circumstances. Such environmental changes in their fundamental effects upon accounting differentiate the meaning of a accounting measurement. Accounting reports by heterogeneous measurements has diminished in informative worth of accounting measurements because there are not secured comparability. Thus, as far as possible, accounting reports should be integrated to one financial statements, identically combined from different home and foreign accounting measurements. The reason that problems of accounting reports of multinational corporation has emerged into a new problematic field is to develop the theory and method to identify different accounting measurements. This paper is to suggest more reasonable directions of reporting system of accounting for multinational corporation, and to explore theoretical foundations of aaccounting report by clarifying the basic problems and characteristics of accounting standards of some countries and their reporting practices. For this purpose, this paper focuses on the discussion of the main subject as follows; (1) The sources and characters of the problems of accounting reports that emerged by the nature and characteristic activities of multinational corporation. (2) Analysis of some country's current accounting standards and reporting practices of multinational corporation. (3) More reasonable model building for accounting report with a view of external and internal management reports.

Bacillus licheniformis KFB-C14가 생산하는 내열성 Chitinase의 정체 및 특성

홍범식,윤호근,신동훈,조홍연 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Bacillus licheniformis KFB-C14가 생산하는 내열성 chitinase를 30~70% ammonium sulfate fractionation, DEAE-Toyopearl 650M, Butyl-Toyopearl 650M, TSK-Gel Toyopearl HW-55F에 의해 정제도 66배, 수율 21%로 전기영동적으로 균일하게 정제하였다. 정제 단백질은 gel permeation chromatography에 의해 86,000±2,000의 분자량을 나타내었으며, SDS 전기영동에 의해 밝혀진 본 효소의 subunit 구조는 monomer였다. 효소 단백질의 안정성을 검토한 결과 80℃에서 30분 열처리에 의해 56%, 37℃에서 20분간 40% ethanol과 ethyl acetate, 단백질 변성제 등의 처리시에도 50% 이상의 잔존활성을 나타냄으로써 공업적으로 유용성이 높은 안정한 단백질로 판명되었다. 효소반응의 최적 pH와 온도는 pH 6.0과 60℃이었고 Mn^2+ 이온에 의해 효소 활성이 저해되었으나 EDTA, N-ethylmaleimide, p-chloromercuribenzoate 등에 의한 활성감소는 관찰되지 않음으로써 금속효소 또는 thiol계 효소에 속하지 않음을 알 수 있었다. 본 효소는 colloidal chitin, 시판용 chitin에는 반응성이 높았으나 exo형 chitinase의 대표적인 기질인 p-nitrophenyl-2-aectamido-2deoxy-β-glucopyranoside, NN'-diacetylchitobiose에는 전혀 반응성을 보이지 않는 전형적인 endo형의 chitinase였다. 본 효소는 colloidal chitin으로부터 주로 (GlcNAc)_2를, 반응시간 경과에 따라 (GlcNAc)_1과 (GlcNAc)_3을 생성하는 반응성을 보였다. Chitinase (EC 3.2.1.14) from culture fluid of Bacillus licheniformis KFB-C14 was purified 66-folds to homogenity in overall yield of 21% by ammonium sulfate fractionation, DEAE-Toyopearl, Butyl-Toyopearl and TSK-Gel HW-55F column chromatography. The enzyme protein had a molecular weight of about 86,000 and was composed of one subunit. The enzyme was significantly stable not only at high temperature but also on treatment with organic solvents and protein denaturants such as SDS, urea and guanidine·HCl. The optimum temperature and pH for reaction was 60℃ and 6.0,respectively. The enzyme activity was inhibited by only Mn^2+ ion, but not inhibited by EDTA, N-ethylmaleimide and pCMB. The enzyme had high activity with colloidal chitin (V_max: 421) and commercial chitin (V_max :480), but not with typical substrates of exo type chitinase. The thermostable chitinase had an useful reactivity for producing functional chitooligosaccharide, showing the production of (GlcNAc)_1, (GlcNAc)_3, and (GlcNAc)_2 as major product.

步行에 關한 文獻的 考察

김범철,금동호,이명종 동국대학교 한의학연구소 1996 東國韓醫學硏究所論文集 Vol.5 No.-

When we see normal gait, gait cycle is seperated as stance phase and swing phase. It needs 6 determinant of gait of pelvic rotation, pelvic tilt, knee joint of stance phase, ankle and foot motion, ankle and knee motion, and pelvic movement to be accomplished. In addition a joint and muscle action is accomplished biomechanically at the same time with its gait cycle. In oriental medicine, the relationship between chang-fu physiology and meridian physiology are summaried as follows : · chang-fu physiology : Spleen manages the extremities Liver manages sod tissues. Liver stores blood. Kidney stores essences. Kidney manages bones. · meridian physiology : The Leg Greater Yang Meridian and meridian soft tissues The Leg Yang-Myeong Meridian and meridian soft tissues The Leg Lesser Yang Meridian and meridian soft tissues The Leg Greater Yin Meridian and meridian soft tissues The Leg Lesser Yin Meridian and meridian soft tissues The Leg Absolute Yin Meridian and meridian soft tissues Especially, we can find out relation between in a "blood supplied feet can walk well" that explains "blood regulations and by liver nourishing affects"that is the closest concept of muscle. Abnormal gaits are due to three causes as following: first, physical defect second, pain third, nervous system or instability of muscle. In oriental medicine, we can know relationship in "atrophy, numbness, stroke, convulsion, muscular dystrophy of knee, rheumatoid arthritis, five causes of infantile growing defects, five causes of softening, sprain". Especially, atrophy is the most important symptom. Gait evaluation should be emphasized where a point can walk 8 feet to 10 feet considering stride width, stride length, the body weight center, stride number, flexion, extension, rotation of a joint as a standard factor. The point is we should find out something strange in a patient's side, front and back view. After that we should find out its cause as an index that we can observe abnormal findings in a joint and muscle.

3-deoxyglucosone에 의한 단백질의 modification

신동범,오상환,양융 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.1

pH 7.4, 37℃의 생리적 반응조건에서 HSA, ovalbumine, BSA, lysozyme, RNase A 및 IgG와 3-deoxyglucosone을 반응시키고 단백질의 중합현상과 아미노산 잔기의 손상상태를 연구하였다. 3-deoxyglucosone은 모든 단백질을 중합시킬 수 있으나 특히 lysozyme, RNase A 및 IgG를 현저하게 중합시켰다. 또한 Arginine잔기를 크게 손상시켰다. glucose에 의한 단백질의 중합과정은 glucose가 단백질의 아미노 그룹과의 반응으로 3-deoxyglucosone을 형성시키고, 이것이 단백질의 lysine 잔기와 arginine 잔기를 공격하여 cross link를 형성시키는 것으로 추정되었다. Human serum albumin, bovine serum albumin, ovalbumin, IgG, lysozyme and ribonuclease A were reacted with 3-deoxyglucosone under physiological conditions of 37℃ and pH 7.4, and polimerization of proteins and impairments of amino acid residues were investigated. Proteins tested, especially lysozyme, IgG and ribonuclease A, were polymerized significantly, and lysine residue and arginine residue, espectially arginine residue of proteins were impaired remarkably. Experimental results strongly suggested 3-deoxyglucosone, formed from proteins-glucose reaction system, was the cross-linker responsible for the polymerization of proteins.

Glycatoin에 의한 단백질중합체의 가교구조의 탐색

신동범,홍상칠,신완철,윤성식,양융 한국노화학회 1992 한국노화학회지 Vol.2 No.1

다중화된 광섬유 BG 센서에 의한 변형률의 측정

권일범,김치엽,윤동진,이승석 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

FBG(Fiber Bragg grating) sensor, which is one of the fiber optic sensors for the application of smart structures, can not only measure one specific point but also multiple points by multiplexing techniques. We have proposed a novel multiplexing technique of FBG sensor by the intensity modulation of light source. This technique is applicable to WDM(Wavelength Division Multiplexing) technique and number of sensors in this system can be increased by using this technique with WDM technique

금합금의 프라이머 처리가 교정용 브라켓의 접착에 미치는 영향

류동범,권태엽,권용훈,김교한 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.3

The purpose of this study was to compare the bonding strength and the interfacial micromorphology of two surface modification of gold alloys in direct bonding of orthodontic brackets. Flat tab of cast gold alloys (n= 120) were subjected to either of two surface treatments: 1.50㎛ aluminum oxide sandblasting, and 2. sandblasting plus adhesive primer for noble metal. Orthodontic brackets (RMO Co.) were bonded directly with Superbond C&B (Sun Medical Co. Ltd.), or Transbond XT (3M Unitek), or Ortho One (Bisco). All specimens were stored in distilled water at 37℃ for 24 hours, and then the tensile bond strength testing was performed in a Instron test machine. Bond failure sites were classified by a modified Adhesive Remnant Index (ARI) system. The results showed that sandblasting plus adhesive primer for noble metal produced stronger bonds to gold alloy than sandblasting only in all cases (Superbond C&B, Transbond XT, Ortho One). Superbond provided significantly stronger bonds to gold alloy than the others. Bond failure of Superbond to sandblast only and sandblast plus adhesive primer for noble metal to gold alloy occurred at the bracket/adhesives interface, whereas bond failure of the others occurred at the gold/adhesive interface. The clinical implications of these findings are discussed.

혈액보존제 ACD-B,CPDA-1 및 SAG-M에 보존된 혈액의 혈액학적,생화학적 변화에 관한 연구

나동진,문희주,최범열 서울보건대학 1997 서울보건대학 부설 한국보건과학연구소 논문집 Vol.4 No.1

Backgrounds ; Study as to blood preservative solution is continuousely performed to prolong period of donor blood preservation which will cope with demand increase and autologous transfusion. In Korea, CPDA-1 solution, preserved 35 days, substituded ACD-B(21days), is currently used for anticoagulant preservative solution of blood. But SAG-M solution(42days) were widely used in other contries for longer preservation as RBCs additive solution. Authors compared the survival of RBCs preserved with ACD-B, CPDA-1 anticoagulant preservative solution, SAG-M RBCs additive solution to study changes of hematological and biochemical. Materials and Methods ; Blood were collected from 15 healthy male donors aged 22 to 23years, for three sorts of preservative solution bag made in home(Company Green Cross, Korea) at the same time. 5 of them were drawn into the blood bags containing ACD-B and another 5 into the CPDA-1 bags, the other 5 donors were collected into the blood bags with CPDA-1, plasma was removed by centrifugation serparation at 4,000rpm in 5minutes and replaced by 90 ml of SAG-M additive solution. All of blood bags were stored in blood bank refrigerator at 1 to 60℃. Samples were drawn at 1, 5, 10, 15, 20, 25, 30, 35 and 42days in each through blood bags tubing under sterile condition used SCD(Sterile Connection Device) so as to measure changes of hematological and biochemical. Results ; 1) A TP level is very important preservation index of hematological test. On 1 days, ATP levels of ACD-B anticoagulant preservative solution were measured by 4.85μmol/gHb, CPDA-1 5.05μmol/gHb, SAG-M additive solution 8.91μmol/gHb. After storage for 42days ATP levels ACD-B were measured by 1.54μmol/gHb, CPDA-11.72μmol/gHb, SAG-M 2.36μmol/gHb. 2) Plasma hemoglobin in bloods stored in ACD-B, CPDA-1 and SAG-M were 24.6mg/dl, 24.3mg/dl and 16.7mg /dl, respectively, on 1days, and increased to 221mg/dl, 154.5mg/dl and 146.4mg/dl, respectively, On 42 days. 3) Another hematological study cannot be seen remarkable discrimination between three sorts of preservative solution and preservation days. 4) pH, sodium, chloride and glucose level in bloods stored in ACD-B, CPDA-1 and SAG-M were slightly descresed according to storage days, whereas pottasium and phosphorus concentration increased according to storage days, However, there was no significant difference between bloods stored in three anticoagulants. Conculusion ; In 42 days, SAG-M RBCs additive solution is more excellent than other solution(ACD-B, CPDA-1 anticoagulant preservative sol) and has merit to separate plasma from the unit blood. Therefore we concluded that SAG-M RBCs additive solution should be used to overcome the state of b, SAG-M additive solution 8.91μmol/gHb. After storage for 42days ATP levels ACD-B were measured by 1.54μmol/gHb, CPDA-11.72μmol/gHb, SAG-M 2.36μmol/gHb. 2) plasma hemoglobin in bloods stored in ACD-B. CPDA-1 and SAG-M were24.6mg/dl, 24.3mg/dl and 16.7mg/dl, respectively, on 1 days, and increased to 221mg/dl, 154.5mg/dl and 146.4mg/dl, respectively, On 42 days. 3) Another hematological study cannot be seen remarkable discrimination between three sorts of preservative solution and preservation days. 4) pH, sodium, chloride and glucose level in bloods stored in ACD-B, CPDA-1 and SAG-M were slightly descresed according to storage days, whereas potasium and phosphorus concentration increased according to storage days, However, there was no significant difference between bloods stored in three anticoagulants. Conculusion ; In 42 days, SAG-M RBCs additive solution is more excellent than other solution(ACD-B, CPDA-1 anticoagulant preservative sol) and has merit to separate plasma from the unit blood. Therefore we concluded that SAG-M RBCs additive solution should be used to overcome the state of imported plasma in our country.