HCV-Specific Interleukin-21+CD4+ T Cells Responses Associated with Viral Control through the Modulation of HCV-Specific CD8+ T Cells Function in Chronic Hepatitis C Patients

Guo-Hua Feng,Fu-Sheng Wang,Ji-Yuan Zhang,Qing-Lei Zeng,Lei Jin,Junliang Fu,Bin Yang,Ying Sun,Tianjun Jiang,Xiangsheng Xu,Zheng Zhang,Jinhong Yuan,Liyuan Wu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

Interleukin-21 (IL-21)+CD4+ T cells are involved in the immune response against hepatitis B virus (HBV) by secreting IL-21. However, the role of IL-21+CD4+ T cells in the immune response against chronic hepatitis C (CHC) virus infection is poorly understood. This study aimed to investigate the role of IL-21+CD4+ T cells in CHC patients and the potential mechanisms. The study subjects in-cluded nineteen CHC patients who were grouped by viral load (low, < 106 RNA copies/ml, n = 8; high, > 106 RNA copies/ml, n = 11). The peripheral frequency of HCV-specific IL-21+CD4+ T cells was higher in the low viral load group and was negatively correlated with the serum HCV RNA viral load in all CHC patients. Meanwhile, IL-21+ cells accumulated in the liver in the low viral load group. In vitro, IL-21 treatment increased the expression of proliferation markers and cytolytic molecules on HCV-specific CD8+ T cells. In summary, these findings suggest that HCV-specific IL-21+CD4+ T cells might contribute to HCV control by rescuing HCV-specific CD8+ T cells in CHC patients.

Model updating with constrained unscented Kalman filter for hybrid testing

Bin Wu,Tao Wang 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.6

The unscented Kalman filter (UKF) has been developed for nonlinear model parametric identification, and it assumes that the model parameters are symmetrically distributed about their mean values without any constrains. However, the parameters in many applications are confined within certain ranges to make sense physically. In this paper, a constrained unscented Kalman filter (CUKF) algorithm is proposed to improve accuracy of numerical substructure modeling in hybrid testing. During hybrid testing, the numerical models of numerical substructures which are assumed identical to the physical substructures are updated online with the CUKF approach based on the measurement data from physical substructures. The CUKF method adopts sigma points (i.e., sample points) projecting strategy, with which the positions and weights of sigma points violating constraints are modified. The effectiveness of the proposed hybrid testing method is verified by pure numerical simulation and real-time as well as slower hybrid tests with nonlinear specimens. The results show that the new method has better accuracy compared to conventional hybrid testing with fixed numerical model and hybrid testing based on model updating with UKF.

Identification of drought response genes in Zygophyllum xanthoxylum by suppression subtractive hybridization

Bin Wu,Xiaohua Su 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4

Drought is an important abiotic stress that seriously limits plant growth and crop productivity in some regions of the world. Zygophyllum xanthoxylum, a succulent xerophyte species with excellent adaptability to adverse arid environments, is an excellent model system for the study of the molecular mechanisms behind drought tolerance in xerophytic species. To better understand the molecular basis of Z. xanthoxylum responses to drought stress and to isolate drought tolerance-related genes from Z. xanthoxylum, we constructed a cDNA library from Z. xanthoxylum and screened the library for stress-related genes by PCR-based suppression subtractive hybridization (SSH). A total of 637 expressed sequence tags were generated by sequencing 672 randomly selected clones, resulting in the identification of 338 unigenes comprising 77 contigs and 261 singlets. The unigenes were assigned to 13 functional categories, such as metabolism, disease- or defense-related genes and energyrelated gene. In addition, we analyzed the expression patterns of 24 genes associated with drought stress-responsive pathways by real-time RT-PCR. The results indicated that osmolyte biosynthesis genes, reactive oxygen scavengers, transporters, signaling components and transcription factors play important roles in drought stress responses. This study provides a valuable resource for studying the drought tolerance mechanism of this xerophytic plant species.

Novel flexible styrenic elastomer cation-exchange material based on phenyl functionalized polystyrene-butadiene copolymer

Wu Bin Ying,장지욱,이민우,황택성,이경진,이범재 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.47 No.-

The sulfonated phenyl functionalized polystyrene-butadiene random copolymer was synthesized forapplication asflexible ion-exchange material. The polymer was prepared through phenyl functionalizationvia thiol-ene reaction and sulfonation of the base polystyrene-butadiene copolymer with differentvinyl contents and phenyl contents. Sulfonation on phenyl functionalized polystyrene-butadienecopolymer was carried out in 1,2-dichloroethane at room temperature using the acetyl sulfonate as thesulfonation agent which was prepared from the reaction of acetyl acetate with sulfuric acid. Thesulfonation degrees were adjusted from 7.3 mol% to 24.3 mol% determined by elemental analysis (EA)with great ion exchange capacity (0.60–2.26 meq/g), conductivity (0.47–6.1 mS/cm) and lower wateruptake (5.1–12.8 wt.%). These excellent characteristics lead to great potential using as theflexibleion-exchange material.

EFFECT OF CHARGE DENSITY ON SPRAY CHARACTERISTICS, COMBUSTION PROCESS, AND EMISSIONS OF HEAVY-DUTY DIESEL ENGINES

Bin Yang Wu,Qiang Zhan,Xiao Yang Yu,Wen Yu Gu,Min Zhang,Wanhua Su 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.4

In-cylinder charge density at top dead center is an important parameter of diesel engines and is influenced by intake pressure, intake temperature, and compression ratio. The effects of charge density on fuel spray, combustion process, and emissions were investigated by using a constant volume bomb and a heavy-duty diesel engine. Spray development resistance increased with the increase of the charge density in the constant volume bomb. It was found that short spray penetration was accompanied by a large spray cone angle in the former stage with high charge density. However, the equivalence ratio was lowered and the degree of homogeneity of the mixture was increased in the later stage owing to the rapid interaction of fuel and gas at a high mixing rate. Combining the first law of thermodynamics and the second law of thermodynamics for analysis, as the charge density increased, the gross indicated thermal efficiency (ITEg) was improved. However, pumping loss had to be considered with higher charge density. Under this condition, the brake thermal efficiency (BTE) trend was increased initially and decreased subsequently. Under high-load operation (1200 r/min BMEP, 2.0 MPa), the minimum charge density value of 44.8 kg/m3 was found to be reasonable. This charge density was suitable for combustion and brought about minimum exhaust energy and trade-off emissions. Moreover, by analyzing two operation conditions in terms of the maximum BTE with the Miller and the conventional cycles, compression temperature and combustion temperature were reduced in the Miller cycle with the charge density 44.8 kg/m3. A high C p/C v could improve the cylinder exergy/power conversion process by its positive effect of increasing the specific heat ratio. Owing to the interaction between a high C p/C v and exergy loss to heat transfer, the condition with the minimal charge density could produce more piston work.

Study on Crystallization Kinetics of Dynamically-Vulcanized PP/EPDM Blends

Bin Yang,Yan-Li Deng,Xia Ru,Ji-Bin Miao,Ming Cao,Jia-Sheng Qian,Li-Fen Su,Peng Chen,Jing-Wang Liu,La-Xia Wu,Tao Pang 한국고분자학회 2016 폴리머 Vol.40 No.4

Two types of β nucleating agents (β-NAs), aryl dicarboxylic acid amide (TMB-5) and diphenyl phthalate diamine (NT-C), were adopted to modify the polypropylene (PP)/ethylene propylene diene monomer (EPDM) blends, which were prepared by dynamic-vulcanization technology. Wide angle X-ray diffraction (WAXD) and differential scanning calorimetry (DSC) were used to study the crystallization kinetics of PP. Our results showed that the addition of β-NAs can considerably increase the crystallization temperature, and significantly decrease the spherulite size of β-PP (L300). The Jeziorny analysis showed there were ~82% and ~89% of relative crystallinity generated from the primary crystallization in the composites containing TMB-5 and NT-C, respectively. The crystallization half time (t0.5) showed that NT-C improved the overall crystallization rate more effectively than TMB-5. In addition, the peaks of the relative crystallization rate curves were shifted towards higher temperature by 14 and 9℃ with the addition of TMB-5 and NT-C, respectively.

Stability Analysis for Time-delay Systems with Nonlinear Disturbances via New Generalized Integral Inequalities

Bin Wu,Chang-Long Wang,Yong-Jiang Hu,Xiao-Lin Ma 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.6

This paper represents a novel less conservative stability criterion for time-delay systems with nonlinear disturbances. The main purpose is to obtain larger upper bound of the time-varying delay. A suitable Lyapunov- Krasovskii functional (LKF) with triple integral terms is constructed. Then, two new generalized double integral (GDI) inequalities are proposed which encompass Wirtinger-based double inequality as a special case. A simple case of the proposed GDI inequality is utilized to estimate double integral terms in the time derivative of the constructed LKF. Further, an improved delay-dependent stability criterion is derived in the form of linear matrix inequalities (LMIs). Finally, some numerical examples are given to illustrate the improvement of the proposed criteria.

Synthesis of poly[2-(3-butenyl)-2-oxazoline] with abundant carboxylic acid functional groups as a fiber-based sol–gel reaction supporter for catalytic applications

Wu Bin Ying,배기현,고나영,김수희,류삼곤,Jin Zhu,Ruoyu Zhang,이범재,이경진 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

Polymer-based catalysts have been of special interest because of their several advantages such as easyprocessibility. Inorganic catalyst generally have been introduced into polymer-based media to endowcatalytic activity. Surface decoration or sol–gel reaction have been performed so far, but inherent lowaffinity between organic/inorganic causes low efficiency in loading amount, resulting in low catalyticactivity. Herein, we synthesized easily processible and highly functionalizable polyoxazoline as polymernonwovenfiber-based catalytic supporter. Zr(OH)4 was grown on surface offiber-web via sol–gelreaction. The Zr(OH)4 grownfiber exhibited not only excellent mechanical properties but also effectivedecontamination efficiency against organophosphate-based toxic agents.

A Convenient Dual-Side Anionic Initiator Based on 2,6-Luditine/s-Butyl Lithium

Wu Bin Ying,고나영,Chen Kai Yao,곽노훈,Ruoyu Zhang,이경진,이범재 한국고분자학회 2019 Macromolecular Research Vol.27 No.6

A novel convenient dual-side initiator of lutidine dianion for anionic polymerization has been investigated in terms of their initiation and propagation kinetics. This lutidine dianion could be synthesized in quantitative yields reacting commercial products of 2,6-lutidine with sec-butyl lithium without any complex organic synthesis, and could be used effectively as the dual-side initiator to synthesize polyisoprene with various molecular weights via living anionic polymerization. In addition, this lutidine dianion can produce 50% of 1.4-microstructure contents in polyisoprene which is very important for elastomeric property of polydienes. All experimental evidences showed the living property and were consistent with quantitative yields without any side reactions when the anionic living polymerization proceeded using lutidine dianion as the initiator.

Nonlinear resonance of porous functionally graded nanoshells with geometrical imperfection

Wu-Bin Shan,Gui-Lin She 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.4

Employing the non-local strain gradient theory (NSGT), this paper investigates the nonlinear resonance characteristics of functionally graded material (FGM) nanoshells with initial geometric imperfection for the first time. The effective material properties of the porous FGM nanoshells with even distribution of porosities are estimated by a modified power-law model. With the guidance of Love’s thin shell theory and considering initial geometric imperfection, the strain equations of the shells are obtained. In order to characterize the small-scale effect of the nanoshells, the nonlocal parameter and strain gradient parameter are introduced. Subsequently, the Euler-Lagrange principle was used to derive the motion equations. Considering three boundary conditions, the Galerkin principle combined with the modified Lindstedt Poincare (MLP) method are employed to discretize and solve the motion equations. Finally, the effects of initial geometric imperfection, functional gradient index, strain gradient parameters, non-local parameters and porosity volume fraction on the nonlinear resonance of the porous FGM nanoshells are examined.