Structure-Activity Relationships of Polyhydroxyursane-type Triterpenoidson the Cytoprotective and Anti-inflammatory Effects

정현주,Jung-Hwan Nam,이경태,이용섭,최종원,김원배,정원윤,박광균,박희준 한국생약학회 2007 Natural Product Sciences Vol.13 No.1

Eleven polyhydroxyursane triterpenoids (PHUTs) were tested to determine their cytoprotective, immunosuppressive and anti-inflammatory effects. To compare the bioactivities of 19α -hydroxyursane-type triterpenoids {23-hydroxytormentic acid (6), its methyl ester (7), tormentic acid (8), niga-ichigoside F 1 (9),euscaphic acid (10) and kaji-ichigoside F 1 (11)} of the Rosaceae crude drugs (Rubi Fructus and Rosa rugosae Radix) with PHUTs possessing no 19α−hydroxyl of Centella asiatica (Umbelliferae), the four PHUTs, asiaticoside (1), madecassoside (2), asiatic acid (3), and madecassic acid (4) were isolated from C. asiatica and 23-hydroxyursolic acid (5) from Cussonia bancoensis. Cytoprotective effects were assessed by measuring cell viabilities against cisplatin-induced cytotoxocity in LLC−PK 1 , cells (proximal tubule, pig kidney) to determine whether these agents have protective effects against nephrotoxicity caused by cisplatin. The inhibitory effect of 11 PHUTS on nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) were evaluated by measuring nitrite accumulation in lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells, and their anti-inflammatory effects were tested in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema model. Six MHUTs (compounds 1, 2, 4, 6, 10, and 11) exhibited higher cell viabilities during cisplatin-induced cytotoxicity testing even at a concentration of 200μg/ml than cisplatin only-treated group, suggesting that ese compounds have the potentcytoprotective efffcts. Compounds 1 and 3 of the C. asiatica and niga-ichigoside F 1 exhibited no inhibitory effect on NO and/or PGE 2 production whereas other PHUTs produced mild to significant NO and/or PGE 2 production.The four compounds (2, 5, 9, and 10) potently inhibited mouse ear edema induced by TPA whereas two compounds (1 and 3) had no activity in this test. These results suggest that many PHUTs are potentchemopreventives. Structure-activity relationship (SAR) was also discussed in each assay with regard to the significant role of OHs at the position of 2, 3, 6, 19, and 23 and to the glycoside linkage at the 28-carboxyl.

사향(麝香) 및 사향(麝香) 배합(配合) 한약제제(韓藥製劑)의 구강(口腔) 투여(投與)가 백서(白鼠)의 기억(記憶) 및 뇌기능(腦機能) 활성(活性)에 미치는 영향

정현주,이유경,채중원,Chung, Hyun-Ju,Lee, Yu-Kyung,Chae, Jung-Won 대한한방소아과학회 2009 대한한방소아과학회지 Vol.23 No.1

Objectives This study was investigated to find how the orally administrated Moschus, herbal combination with Moschus, and herbal combination improves the rats' memory and rats' liver. These medications are generally known asthe memory improvement. Methods This study used the Sprague Dawley rats. They were divided into two groups - SD rats and orally administrated Saline(Control group). 0.473 mg/kg Moschus(HM-A), 153.9 mg/kg herbal composition without Moschus(HM-B), and 165.95 mg/kg herbal composition with Moschus combined(HM-C) Control, saline were orally administered. Each group was trained in the eight-arm radial maze task at the conditions of before oral administrated, and also right after third, sixth, and eighth by oral administration. Lastly, these animals were killed and were tested for brain tissue and serum AST/ALT level to measure how the medications were effected to the liver function. Results The result of radial eight-arm maze task test, the HM-B and HM-C groups showed significant decrease in mistakes from the fourth day of testing. Whereas, the HM-A group showed increasing in the error rate. HM-A and HM-C group of rats had significantly increased amount of acetylcholinesterase in the CA1 region of hippocampus, compared to the control group. Whereas, HM-B and HM-C group had increased level of ChAT compared to the control group. On the other hand, each experimental group did not show any significant difference to the level of serum AST/ALT and the weight ratio of the liver to the body. Conclusions This study provided evidences that the orally administered memory improvement herbal medication, and Moschus were effective to improve memory.

A군 연쇄구균 무증상 감염자에서 페니실린 투여 후 세균제거 효과에 대한 조사

정현주,김선주,Jung, Hyun-Ju,Kim, Seon-Ju 대한소아감염학회 1998 Pediatric Infection and Vaccine Vol.5 No.2

목 적 : A군 연쇄구균 무증상 감염자는 증상은 없지만 A군 연쇄구균이 분리되고 ASO(antistreptolysin O)치가 높은 사람으로서 이들은 류마티스열이나 사구체신염 등 후유증을 일으킬 수 있다. 저자들은 무증상 감염자를 대상으로 경구용 페니실린을 복용하게 하여 이들에서 페니실린 치료 효과를 조사하였다. 방 법 : 상기도 감염 증상이 없는 건강한 초등 학생들을 대상으로 인두배양과 ASO치를 정량적으로 측정하였다. A군 연쇄구균이 분리되고 ASO 치가 400IU/mL 이상인 아동 36명을 대상으로 하여 그 중 22명의 학생은 경구용 페니실린 제제(penicillin V, 500mg. 하루 2회)를 10일간 복용하게 하였고, 14명은 대조군으로 삼고 항생제를 복용하지 않았다. 한달 후 두 그둡 아동에서 동시에 인두배양을 시행하여 A군 연쇄구균 제거율을 비교하고 ASO치를 측정하여 농도 변화를 관찰하였다. 결 과 : 항생제를 복용한 그룹과 복용하지 않은 그음의 A군 연쇄구균 제거율은 각각 91%(20/22)와 50%(7/14)로서 항생제를 사용한 그룹에서 유의하게 높았다(P<0.05). 항생제를 사용한 그룹과 사용하지 않은 그룹에서 ASO치가 100IU/mL 이상 상승한 경우는 각각 22%(4/18)와 30%(3/10)이었고 200IU/mL이상 감소한 경우는 각각 44.4%(8/18)와 40%(4/10)로서 유의한 차이가 없었다. 결 론 : 무증상 감염자를 대상으로 페니실린을 투여했을 때 인두에서 균이 효과적으로 제거되어 페니실린 치료의 타당성을 확인하였다. 그러나 ASO치 감소는 두 그룹간에 유의한 차이가 없었는데, 이는 ASO의 반감기가 걸고 경구용 페니실린의 경우 순응도가 낮기 때문으로 사료된다. 항생제 치료에도 불구하고 ASO치가 상승한 것을 치료 실패로 판정할 때 22%의 치료 실패율을 보였다. Purpose : Asymptomatic infections with positive throat culture for group A beta-hemolytic streptococci(GABHS) and high antistreptolysin O(ASO) concentration may lead to sequelae such as rheumatic fever or acute poststreptococcal glomerulonephritis. Children with asymptomatic infections were treated with oral penicillin V to evaluate the effectiveness of penicillin treatment on the asymptomatic infections. Methods : Throat culture and ASO concentration analysis were performed against healthy elementary school children. Thirty-six children with positive throat culture for GABHS and ASO concentrations of 400 IU/mL or more were divided into two groups. Twenty-two children were treated with oral penicillin V for 10 days, and the others were not treated. Eradication rate of GABHS and the change of ASO concentration between the two groups were compared after one month later. Results : Eradication rates of GABHS between treated and untreated children were 91%(20/22) and 50%(7/14) respectively(P<0.05). Children showing elevation of ASO levels more than 100IU/mL were 22%(4/18) in the treated group and 30%(3/10) in the untreated group, while children showing a decrease of more than 200IU/mL in the ASO level were 44%(8/18) and 40%(4/10) respectively. Conclusion : We confirmed the validity of penicillin treatment, because when we treated the asymptomatic children with penicillin V, the GABHS was eradicated effectively. But there was no significant difference of decrease in the ASO levels between the two groups due to long half-life of ASO or poor compliance. Treatment failure was 22% in terms of elevated ASO levels after penicillin treatment.

교류 자기센서 특성 시험장치 설계에 관한 연구

정현주,양창섭,정우진,Chung, Hyun-Ju,Yang, Chang-Seob,Jung, Woo-Jin 한국군사과학기술학회 2015 한국군사과학기술학회지 Vol.18 No.3

This paper describes design and construction results of the measurement system developed on the purpose of measuring properties of AC magnetic field sensors used in the weapon system. The system for measuring the properties of AC magnetic field sensors consist of 3-axis helmholtz coil, signal generator, signal amplifier, sensor data acquisition unit and AC magnetic field sensor property measurement & analysis equipment including the operating software. By using this system, we can measure various properties of AC magnetic field sensor such as sensitivity, linearity and dynamic response in the frequency from 1 Hz to 10 kHz. Finally we also verified its performance by measuring the property of a MAG 639, standard magnetic field sensor of bartington instruments, with the developed measurement system.

Anti-Ulcerogenic Effects of the Flavonoid-Rich Fraction from the Extract of Orostachys japonicus in Mice

정현주,Jongwon Choi,Jung-Hwan Nam,Hee-Juhn Park 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.4

Orostachys japonicus(Crassulaceae) herbal preparations have been used to treat gastric ulcer or gastric can-cer disease in Korean folk medicine. To demonstrate the effects of the methanol (MeOH) extract of O. japonicusand its frac-tions on gastric lesions and pain, the MeOH extract was fractionated into triterpene-rich and flavonoid-rich (FRF) fractions.Second, the fractions were subjected to analgesic assays including hot plate and writhing assays and anti-ulcerogenic assaysin HCl/ethanol-induced- and indomethacin/bethanechol-induced ulcer models in mice. In this experiment, it was found thatthe FRF most significantly reduced ulcerative indices and pain in mice, although the MeOH extract was also effective. Oraladministration of the FRF highly reduced the diameter of gastric lesion induced by HCl/ethanol (inhibitory effect, 53%) andby indomethacin/bethanechol (inhibitory effect, 36%) at the 100 mg/kg dose. In addition, oral administration of 200 mg/kgFRF markedly increased the reaction time in the hot plate test by 52% and decreased stretching episodes (45%) in the writhingtest. These results suggest that the active component of O. japonicusexhibiting the potent anti-ulcerative and antinociceptiveeffect is included in the FRF. The anti-ulcerative effects of the MeOH extract and the FRF were also supported by gastricjuice and gastric acid volumes and pH in pylorus-ligated mice. Taken together, these results provide evidence-based supportfor the traditional use of O. japonicusfor gastric disease.

천공성 모낭염

정현주,이영헌,정상립,김도원 ( Hyeon Ju Jung,Young Hun Lee,Sang Lip Chung,Do Won Kim ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.5

해도형 초극세사를 사용한 인조 스웨이드용 기포의 제조 특성에 관한 연구

정현주,장선호,심현주,Jung, Hyun-Joo,Chang, Sun-Ho,Shim, Hyun-Joo 한국섬유공학회 2007 한국섬유공학회지 Vol.44 No.1

For the purpose of enhancing the quality of artificial suede, a fiber web was prepared using chopped seaisland type micro fibers dispersed in the water bath. An apparatus equipped with a laser beam was developed to evaluate state of the fiber dispersion in the water. A woven scrim fabric was sandwiched between these isotropic fiber webs produced through this method and then this was interlaced by water jet punching method. To dissolve the sea part of the micro fibers, alkaline finishing was applied on this composite and then the artificial suede primary fabric was prepared. For comparison, the other artificial suede primary fabrics were prepared such as applying carding method for the preparation of fiber web and needle punching method for the interlacing. After that, alkaline treatment was done for this fabric same as the above ones. The tensile properties, tear properties, surface and cross-sectional structures of these primary fabrics were studied. The hand values of these were also compared using KES-F system. The artificial suedes made of fiber webs by wet method interlaced through spunlacing showed superior isotropicity and hand values than the fiber webs made by carding method interlaced through needle punching.

소아과 의원을 방문한 급성인두염 환자의 인후배양과 emm 유전자형을 이용한 A군 연쇄구균의 역학조사

정현주,이남용,권오영,맹국영,김선주,Jung, Hyun Ju,Lee, Nam Yong,Kwon, Oh-Young,Maeng, Kook Young,Kim, Sunjoo 대한소아감염학회 2003 Pediatric Infection and Vaccine Vol.10 No.2

목 적 : 소아과 의원을 방문한 인두염 환자에서 A군 연쇄구균 분리율을 살펴보고자 하였다. T 항원형과 emm 유전자형을 동정하여 역학적 특성을 살펴보고, 같은 지역의 보균자 결과와 비교하고자 하였다. 방 법 : 2001년 11월부터 2002년 5월까지 경남 진주시에 위치한 한 소아과 의원에서 인두염 환자 246명(남자 123명, 여자 123명)을 대상으로 인후배양을 시행하였다. 슬라이드 응집법으로 T 항원형을 결정하였고, emm 유전자 증폭 후 염기서열 분석을 시행하여 emm 유전자형을 동정하였다. 결 과 : 인두염 환자 246명 중 130명(52.8%)에서 베타용혈성 연쇄구균이 분리되었으며, 그 중 96.1%가 A군이었다. 연령별로는 4세에서 7세가 70.4%로 가장 많았다. T12가 35.2%로 가장 흔하였고, T non-typeable 30.4%, T28 14.4% 순이었다. emm12(28.5%)가 가장 많이 동정되었고, emm75(18.7%), emm22(13.0%), emm2(12.2%) 및 emm8(8.1%) 순이었다. 결 론 : A군 연쇄구균 인두염은 약 50%에서 분리될 정도로 흔하므로, 개인 소아과 의원에서 급성인두염 환자에 대해 항생제 처방 전에 인후배양이 필요할 것으로 사료된다. T항원 및 emm 유전자형은 급성 인두염 환자와 보균자에서 비슷한 분포를 보였다. Purpose : Group A streptococci(GAS) was isolated from the patients with acute pharyngitis. Epidemiological studies using T typing and emm genotyping was performed for GAS and compared with the results of the carriers. Methods : Throat cultures were taken from 246 children(123 boys, 123 girls) from November, 2001 to May, 2002 who visited a pediatrician's office located in Jinju, Gyeongnam province. T types were identified with slide agglutination and emm genotypes were identified with DNA sequencing after amplification of emm genes. Results : One hundred thirty(52.8%) out of 246 children yielded beta-hemolytic streptococci, of which 96.1% were group A. Children from 4 to 7 years old comprised 70.4% of the GAS positive group. T12 were the most common(35.2%) and T non-typeable strains were the next(30.4%). emm12 was most frequent(28.5%), and emm75(18.7%), emm22(13.0%), emm2(12.2%), and emm8(8.1%) were relatively common. Conclusion : Since GAS is so highly prevalent in acute pharyngitis, indeed being half of the population, good clinical practice dictates the systematic employment of throat culture for acute pharyngitis before prescribing antibiotics in a pediatric setting. The distribution of the T antigens and emm genes showed similar pattern between the acute pharyngitis and the carriers.

젠트리피케이션의 이론과 쟁점: 비교 연구를 통한 맥락적 분석

정현주 ( Hyun Joo Jung ) 서울대학교 지리교육과 2005 地理敎育論集 Vol.49 No.-

한국적 의사소통 집단상담 프로그램이 전입신병의 인간관계와 군 생활 적응에 미치는 효과

정현주 ( Hyun Ju Jung ),권재철 ( Jae Chul Kwon ),전은령 ( Eun Ryoung Jeon ),차영은 ( Young Eun Cha ),민경옥 ( Kyung Ok Min ) 국군간호사관학교 군진간호연구소 2014 군진간호연구 Vol.32 No.2

Purpose : The population group of study was newly enlisted soldiers who just finished the basic military training. The study focused on influence of a korean style communication group counseling program on our population group`s human relations and adaptability in a military environment. Methods : This program recommended in the study composed of professional military counseling activity and operational manual. Throughout the two preliminary experiments, we decided to use above on experimental group and another general style used program on comparative group. The program was executed on unit A. We measured communication ability, human relations and adaptability before and after the experiment. In each group, there were 19 applicant soldiers per the minimum of cohen`s formular. Results : After ANCOVA analysis, the result demonstrated that experimental and comparative groups scored higher points in communication ability and adaptability than control group. Especially, the significance in human relation from experimental group was quite different from that of last study. Conclusion : The program influenced new enlisted soldiers in a positive way. Therefore the study has been achieving a changeable goal and also assured that professional counseling for enlisted soldiers may adapt and utilize the program to prevent soldiers who trouble adapting in service.