대전지역 대학병원에서 동정된 비예기항체의 분포와 빈도

강희정 ( Hee-jung Kang ),임춘화 ( Chun-hwa Ihm ),이문희 ( Moon-hee Lee ),현성희 ( Sung-hee Hyun ),김인식 ( In-sik Kim ) 대한임상검사과학회 2010 대한임상검사과학회지(KJCLS) Vol.42 No.2

Antibody screening and identification tests before blood transfusion are important because unexpected red antibodies can cause acute or delayed hemolytic transfusion reactions. Although a tube method was used for detecting unexpected antibodies, a column agglutination method has recently been used because of its simple procedure and a high detection of warm antibodies. This study investigated the frequency and distribution of unexpected antibodies in transfusion candidates during the recent 5 years, and transfusion characteristics in the identified cases. From January 2005 to December 2009, 46,923 sera of the cases from E hospital were screened and 98 sera were identified by the DiaMed-ID System. 272 cases (0.58%) showed positive results out of all 46,923 cases that underwent unexpected antibodies screening. Among them, unexpected antibodies were identified in 98 cases. The anti-Rh antibodies included in warm antibodies were the most frequently detected in 47 cases (47.96%). Anti-Lewis and anti-MNSs antibodies were detected in 11 cases (11.22%) and 6 cases (6.12%), respectively. Unidentified antibodies were detected in 6 cases (6.12%). Among the patients with unexpected antibodies, 43 cases (43.88%) had a history of previous transfusion. Anti-E was the most frequently detected antibody (4/14 cases, 30.77%) in the cases who had a previous history of transfusion and showed different screening results from negative to positive, This study may provide the basic data for the frequency and characteristics of red cell antibodies.

유방 침범을 동반한 원발성 자궁경부림프종 1예

김내유 ( Nae Yu Kim ),조인성 ( In Sung Cho ),공수정 ( Soo Jung Gong ),이미란 ( Mee Ran Lee ),박미자 ( Mee Ja Park ),임춘화 ( Chun Hwa Ihm ),이정애 ( Jung Ae Lee ) 대한내과학회 2008 대한내과학회지 Vol.74 No.4

자궁경부에서 발생하는 악성 림프종은 그 발생이 매우 드물고 질도말검사 등으로 진단이 어려우므로 진단이 늦어져 적절한 치료시기를 놓칠 수 있다. 질출혈, 골반동통 등의 자궁병변을 의심할 수 있는 증상을 보일때 림프종의 가능성을 염두에 두고 조직검사를 통한 조직학적, 면역학적인 확진이 필요하고, 이차 병소의 침범을 확인하기 위하여 양전자 방사 단층 촬영 등을 통한 정확한 병기 결정이 필수적이라 생각한다. 저자들은 성교 후 비정상 질출혈을 주소로 내원한 32세 여자 환자에서 유방 침범을 동반한 미만성 대세포형의 원발성 자궁경부림프종 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Primary lymphoma arising from the uterine cervix has been rarely encountered, and breast involvement is rare because of the relative paucity of lymphoid tissue in the breast. A 32-year-old woman with a primary malignant lymphoma of the uterine cervix and breast involvement is reported. The patient presented with post-coital vaginal bleeding, and punch biopsy of the cervix revealed the diffuse large B cell type of malignant lymphoma. PET-scan was done for staging, and abnormal FDP uptakes were detected in a uterine cervical mass and breast nodule. Ultrasonography-guided needle biopsy was done for the breast mass, and 2 biopsied nodules revealed fibroadenoma and diffuse large B cell lymphoma. The patient (Ann Arbor stage IV) was treated with 6 cycles of combination chemotherapy with CHOP plus rituximab. The patient went into complete remission. Thereafter, 4500cGy pelvic irradiation was done for adjuvant therapy.(Korean J Med 74:435-440, 2008)

21q22 부위의 중복을 보인 다운증후군 1예

임춘화,김문희,신소영,구선회,박종우 대한임상병리학회 1999 대한임상병리학회지 Vol.19 No.4

뇌종양세포의 세포유전학적 고찰

구선회,임춘화,전영미,김성호 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

Cytogenetic results and genetic alterations are closely related with tumorigenesis, progression and promotion of cancers. With the deepening of basic biologic understanding of neoplastic mechanisms, the clinical usefulness of various cytogenetic abnormalities as diagnostic and prognostic aids has been appreciated. Ten cases of brain tumors(2 Glioblastoma multiforme, 3 meningioma, 1 ependymoma, 1 gliosarcoma, 1 astrocytoma, and 1 metastatic tumor) were studied and 8 cases were possible for karyotyping . Four patients showed abnormal karyotypes. Structural and numerical abnormalities were detected. Karyotyping of 2 Glioblastoma multiforme showed trisomy 7, loss of chromosome 10 and loss of chromosome 8. Structural anomalies were detected on chromosome 1q, and 7. Many many marker chromosomes were also detected. One gliosarcoma showed many numerical anomalies, such as +7, +8, +17, and +mar. Meningioma cells studied were normal daryotyping. Although the cases were too small to correlate with the tumorigenesis, but histologically poor grade type of tumors showed very complex karyotypes.

임상화학 검사의 Delta Check System 개발 및 정도관리 효율성 검토

권계철,임춘화,김문희,박연보 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : The application of the delta check in tests in general chemistry detects both random and clerical errors, thus enhancing the reliability of tests. However, since this approach adds the burden of confirming the results to the laboratory, it has the disadvantage of lengthening the turn around time of tests. We speculated that an online delta check system needed to reduce the turn around time. We developed delta quality control system and evaluated its effectiveness of quality control in clinical chemistry. Methods : We developed an online delta check system based on the client-server paradigm. We used IBM PentiumⅢ PC as server and as clients. The database system used was powerbuilder 6.0. Results : In the system we developed, delta screening was performed when test results were input to the computer. The decision over the delta screening was made by comparing it against patient's clinical information and cumulative results within the same screen. Conclusions : The developed delta check method made it available to reduce the turn around time previously spent on delta screening by eliminating the batch processing of tests which was needed in previous approaches separate query cumulative results and patient's clinical informations for screening purposes.

비교유전자교잡법과 형광동소교잡법을 이용하여 기원을 밝힌 Marker 염색체 1예

신소영,전영미,임춘화,김문희,구선회,박종우,권계철,박윤미 대한임상병리학회 2001 대한임상병리학회지 Vol.21 No.4

미세절제술과 비교 유전자 보합법에 의한 각종 종양에서의 유전자 변화에 관한 연구

구선회,신소영,임춘화,전영미,이윤이,김진만 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

For the evaluation of oncogenesis, progression and prognosis of cancer, CGH is an important technique, because this technique is economic due to utilization of only one probe and lack of culture, screening mathod of whole genome and possibility of retrospective and prospective study. By the CGH, genornic variation of 20 breast cancer tissues, 23 stomach cancer tissues and 16 bladder cancer tissues were analyzed. The results were as followes ; 1. breast cancers The CGH results showed gains on chromosomes 8q(40%), lq(30%), 17q(15%), 20q(15%), 18q (15%), 5p(15%), and 13q(15%). The Deletions were on chromosomes 17p(45%) and 22q(20%). High-level amplifications(green/red ratio >1.5) were noted on chromosomes 1p31, iq, 3q25-qter, 5p, 7q31-qter, 8q, 9q22-qter, 10p, l1p, 11q22-qter, 12p, 12q24, 14g21-qter, 15q23-qter, 17q, 18p, 18q12-qter, 20p, and 20q. By comparison with infiltrating ductal carcinoma, the two medullary carcinomas showed high-level amplification on chromosomes iq3l, lq, 8q, 10p, 11p and 12p. 2. stomach cancers 1) Usual amplification sites of genome were lq, 13q, 17q, 20p,q. 2) 17p was the most common deletion site. The other sites of the deletion were lq, 4q. 3) In intestinal type of stomach cancer, genomic variation is more common than diffuse type. 4) In the cases of no evidence of lymph node metastasis, deletion of 17p is absent but amplification of 8q is obvious in the case of lymph node metastasis. 3. bladder cancers Common amplification of copy numbers of DNA sequences by CGH were seen at 1q, 3q, 4q, 5p, 6pq, 7p, 8q, 11q, 12q, 13q, 17q, 18q and 20pq(more than 20% of cases). High level amplification was noted at 1p32, 3p2l, 3q24, 4q26, 8q21-ter, 11q14-22, 12q15-21, 12q21-24, 13q 21-31, 17q22, and 18q22. Deletions were noted at 2q21-qter, 4q13-23, 5q, 8p12-22, 9pq, 11p13-15 (more than 20% of cases).