Meckel 게실의 임상양상

이진범,이용순,유은선,김혜순,손세정,박은애,이승주,성순희,서정완,Lee, Jin Beom,Lee, Yong Soon,Yoo, Eun Sun,Kim, Hae Soon,Son, Se Jeong,Park, Eun Ae,Lee, Seung Joo,Sung, Sun Hee,Seo, Jeong Wan 대한소아청소년과학회 2002 Clinical and Experimental Pediatrics (CEP) Vol.45 No.4

목 적 : Meckel 게실은 장출혈, 장폐색, 감염, 천공등의 합병증에 의한 증상이 나타날 수 있으며 급성 복증을 일으키는 다른 질환과 감별진단이 어렵고 진단이 지연되기 쉽다. 저자들은 Meckel 게실 환자에서 임상증상과 조직학적 소견을 비교하여 신속히 진단하고 치료하는데 도움이 되고자 하였다. 방 법 : 1993년 10월부터 2001년 8월까지 이화의료원에서 진단한 Meckel 게실 10례의 의무기록을 후향적으로 조사하여 임상증상과 조직학적 소견 등을 분석하였다. 결 과 : 1) 환자의 연령은 7일-14세의 분포를 보였고, 6세 이전(60%)이 많았다. 남녀비는 2.3 : 1로 남아에서 많았다. 2) 주요증상은 무통성 하부 장출혈, 복통, 복부 팽만, 구토 순이었고 나이가 많을수록 증상이 심하였다. 3) 수술 전에 Meckel 게실로 진단되거나 의심되어 수술 한 경우 5례, 초음파 유도하 수압으로 정복되지 않은 장중첩증(3례)과 장폐색(2례)으로 수술하여 발견된 경우 5례이었다. 4) Meckel 스캔($^{99m}Tc-pertechnetate$)은 cimetidine을 투여한 후 6례에서 시행되었으며, 이소성 위점막이 있었던 5례 중 4례에서 양성, 1례 위음성, 이소성 위점막이 없었던 1례에서 음성이었다. 5) Meckel 게실은 회맹판으로부터 35-70 cm 상부에 있었고, 길이는 4-12 cm이었으며 이중 80%가 5cm 미만이었다. 6) 하부 장출혈이 있었던 5례 모두에서 이소성 위점막이 발견되었고, 그 외의 조직학적 소견은 염증 3례, 궤양 3례, 천공 1례, 출혈 3례, 경색 1례이었다. 결 론 : Meckel 게실은 출혈량이 적거나 증상이 경미한 환자에서 신속히 진단하게 되면 출혈이나 수혈의 빈도를 줄일 수 있으며 회복도 빠르므로, Meckel 게실을 염두에 두고 조영술이나 내시경에 우선하여 Meckel 스캔을 시행하는 것이 좋다. 이소성 위점막이 없는 Meckel 게실에서는 Meckel 스캔이 음성이므로 임상적으로 의심될 때에는 복강경, 컴퓨터 단층촬영, 고해상도 초음파촬영 등의 다양한 진단법이 시도되어야 할 것이다. Purpose : The diagnosis of Meckel's diverticulum is difficult and delayed because it presents with various clinical symptoms. We evaluated clinical, imaging and pathologic findings of Meckel's diverticulum to facilitate detection of Meckel's diverticulum in children. Methods : Review of clinical, imaging, surgical and pathological findings in 10 children aged 7 days to 14 years with Meckel's diverticulum during an 8-year period, 1993-2001, at Ewha Womans University Hospital was undertaken. Results : The male to female ratio was 2.3 : 1. The chief complaint was painless lower gastrointestinal( GI) bleeding; others were abdominal pain, abdominal distention and vomiting, in order of frequency. The diagonsis before surgery were Meckel's diverticulum in 5 patients, non-reducible intussusception in 3 patients and intestinal obstruction in 2 patients. The diverticulum was located between 35 cm to 70 cm proximal to the ileocecal valve. The length of the diverticulum ranged from 4 cm to 12 cm and 80% of it was within 5 cm. A Meckel scan($^{99m}Tc-pertechnetate$ scintigraphy) after cimetidine administration was done in 6 cases. All 5 cases that presented with lower GI bleeding had ectopic gastric mucosa confirmed on pathology. Out of 5 cases of ectopic gastric mucosa, only 4 cases were positive on the Meckel's scan. Conclusion : In cases of unexplained GI bleeding, obstruction, or inflammation diagnostic workup should be carried out to rule out Meckel's diverticulum. Laparoscopy, high resolution ultrasonography and computed tomography of the abdomen may be indicated in the assessment of pediatric patient with lower GI bleeding, especially in patients with suspected bleeding from Meckel's diverticulum showing negative Meckel's scan.

옥수수 분리 엽록체에서의 엽록소 형광의 소멸과 광계 2 의 Quantum Yield

이진범,박인호,이혜주,선우 양일 ( Chin Bum Lee,In Ho Park,Hae Joo Lee,Yang Il Sun Woo ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6

The relationship between quantum yield of photosystem II and quenching component of chlorophyll fluorecene has been investigated through a series of experiments on isolated maize mesophyll chloroplasts. The thylakoid membranes of higher plants possess several mechanisms that control both the distribution and the rate of dissipation of absorbed light. These mechanisms allow regulation by external and internal factors. By using DCMU addition techniques, there is an advantage to be able to distinguish energy-dependent quenching (qE) from other components of non-photochemical quenching (qNP). At low light intensity, photochemical quenching (qQ) is major component of chlorophyll fluorescence quenching, while at high light intensity, qNP is major one, The relationship between qQ ans quantum yield of oxygen evolution (Φ_s) with incident light intensity was showed. The nonlinear relation is indicative of the decline in the intrinsic yield of photosystem II (Φ_p) at high light intensity. Φ_p declined as the light intensity increased, which was inversely proportional to qE. The oxygen evolution was increased gradually as the temperature increases, but over 45℃, decreased rapidly. qE, qR (the remaining quenching) and qNP were continuously decreased, while qQ was rapidly increased over 30℃. The changes of (F_v)_m,/F_m on temperature may be presumably able to suggest that they are affected by the phophorylation of LHCII. And interestingly, the changes of F_v)_m,/F_m were very similiar with those of Φ_p.

저항 점 용접을 이용한 AISI 316 스테인레스강과 용융아연도금 강판의 이종접합

이진범,남대근,강남현,김양도,오원태,박영도,Lee, Jin-Bum,Nam, Dae-Geun,Kang, Nam-Hyun,Kim, Yang-Do,Oh, Weon-Tae,Park, Yeong-Do 한국재료학회 2009 한국재료학회지 Vol.19 No.7

The spot weldability of dissimilar metal joints between stainless steels (AISI316) and interstitial free (IF) steels were investigated. This study was aimed to determine the spot welding parameters for a dissimilar metal joint and to evaluate the dissimilar metal joint's weldability, including its welding nugget shape, tensileshear strength, hardness, and microstructure. The fracture surface was investigated by using a Scanning Electron Microscopy (SEM). The experimental results showed that the shape of nugget was asymmetric, in which the fusion zone of the STS316 sheet was larger due to the higher bulk-resistance. The microstructure of the fusion zone was fully martensite. In order to evaluate the microstructure further, dilution of stainless steels were calculated and imposed onto the Schaeffler diagram. The predicted microstructure from the Schaeffler diagram was martensite. In order to confirm the predicted microstructure, XRD measurements were carried out. The results showed that that initial weld nugget was composed of austenite and martensite.

Greening 에 따른 보리 어린식물의 전자전달과 광인산화반응 활성의 변화

이진범,홍영남,이순희,조영동,권영명 ( Chin Bum Lee,Young Nam Hong,Sun Hi Lee,Young Dong Cho,Young Myung Kwon ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.1

The development of activities of electron transport and photophosphorylation at various stages of greening was studied with the isolated chloroplast in dark-grown, 7-day-old barley seedlings. The activity of total photosystem(P5 II+I) reached the peak at 4 hrs of illumination, after which it sharply decreased (FeCy, DCPIP, or MV as acceptor) . After 8hrs greening it increased gradually. The activity of photosystem I (PS I) showed the similar pattern with that of PS II+I during the initial greening time, but it was decreased gradually after 8 hrs greening. That of photosystem II (PS II) , however, was increased continuously up to 48hrs. The content of chlorophyll a and b increased abrubtly with greening and the ratio of chl a/b after 8 hrs of illumination reached about 3 : 1. In the absence of photophosphorylation, the pH changes of chloroplast suspension were induced by illumination. In these cases, the acidity per ㎎ chlorophyll was decreased sharply whereas its per mg protein did not show great difference. The photophosphorylation was not detected in the isolated chloroplast within 16 hrs greening, and the pH change produced by ATP formation was observed after 24 hrs greening.

Greening에 따른 보리 어린식물의 전자전달과 광인산화반응 활성의 변화

이진범,홍영남,이순희,조영동,권영명,Lee, Chin-Bum,Hong, Young-Nam,Lee, Sun-Hi,Cho, Young-Dong,Kwon, Young-Myung 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.1

어두운 곳에서 7일간 기른 보리(Hordeum vulgare L. cv. Baecdong) 어린식물을 750 ft.c의 빛에서 greening시킬 때 일어나는 광화학반응 및 광인산반응의 변화를 분리 엽록체를 재료로 조사하였다. PS II+I의 활성은 greening 초기(4hr)에서 최대값을 보인 뒤 급속히 감소하다 8시간 이후부터 점진적으로 증가한 반면, PS I의 활성은 초기에서 보인 최대값은 PS II+I 활성과 같은 양상을 보였으나, 8시간 이후 오히려 감소하는 경향을 보였다. 그러나 PS II 활성은 greening 시킴에 따라 계속적으로 48시간까지 증가하였다. 엽록소 a와 b의 함량은 greening 되면서 급격히 증가하였으며, 특히 chl a/b 값은 8시간 이후에는 거의 3 : 1의 비율이 유지되었다. 광인산화반응이 얼어나지 않는 조건밑에서 엽록체 현탁액의 pH 변화는 빛에 의해 유발되었으며, 엽록소량으로 표시할 경우 acidity는 급격한 감소 현상을 보였고, 단백질량으로 표시한 경우에는 커다란 변화를 나타내지 않았다. 광얀산화반응은 16시간이내의 greening 처리를 한 보리의 분리 염록체에서는 검출되지 않았고, greening 시간을 24시간 이상으로 연장할 때 비로서 뚜렷한 ATP 형성에 의한 pH 변화가 관찰되었다. The development of activities of electron transport and photophosphorylation at various stages of greening was studied with the isolated chloroplast in dark-grown, 7-day-old barley seedlings. The activity of total photosystem(PS II+I) reached the peak at 4 hrs of illumination, after which it sharply decreased (FeCy, DCPIP, or MV as acceptor). After 8hrs greening it increased gradually. The activity of photosystem I (PS I) showed the similar pattern with that of PS II+I during the initial greening time, but it was decreased gradually after 8 hrs greening. That of photosystem II (PS II), however, was increased continuously up to 48hrs. The content of chlorophyll a and b increased abrubtly with greening and the ratio of chl a/b after 8 hrs of illumination reached about 3 : 1. In the absence of photophosphorylation, the pH changes of chloroplast suspension were induced by illumination. In these cases, the acidity per mg chlorophyll was decreased sharply whereas its per mg protein did not show great difference. The photophosphorylation was not detected in the isolated chloroplast within 16 hrs greening, and the pH change produced by ATP formation was observed after 24 hrs greening.

지방간이 동반된 비만아에서 혈청 지방산과 Carnitine 농도에 대한 연구

이진범,이재원,홍영미,Lee, Jin Bum,Lee, Jae Won,Hong, Young Mi 대한소아청소년과학회 2002 Clinical and Experimental Pediatrics (CEP) Vol.45 No.9

목 적 : 지속적으로 증가하는 소아 비만은 성인 비만으로 이행되기 쉽고 합병증으로 고혈압, 지방간, 동맥경화증이 동반될 수 있다. Carnitine은 장쇄 지방산이 미토콘드리아로 이동할 때 필요한 조효소로 지방산 대사에서 중요한 역할을 한다. 지방간을 가진 비만 소아에서 혈중 지방산과 carnitine 농도를 측정함으로써 L-carnitine을 임상적으로 비만 치료에 적용할 수 있는지를 알아보고자 본 연구를 실시하였다. 방 법 : 7-18세의 지방간으로 진단받은 비만아 9명과 정상 대조군 소아 10명을 대상으로 하였다. 혈장을 sodium borate를 섞어 원심분리 후 하층을 methylene chloride를 이용하여 계층 분리하였고, MSTFA와 acetonitrile을 넣고 유도체화 반응을 시켰다. GC-MS 자동 분석기로 혈청 지방산 fraction을 정량 분석하였고, carnitine(free, acyl, total)은 cycling technique을 이용하여 415 nm에서 정량 분석하였다. 결 과 : 혈중 총 지방산은 지방간이 동반된 비만아 군에서 대조군에 비해 유의하게 증가하였고 특히 장쇄 지방산(myristic acid, palmitoleic acid, palmitic acid, linoleic acid, oleic acid, stearic acid)이 의미 있게 증가되어 있었다. 총 carnitine과 유리 carnitine 농도가 비만아에서 정상아에 비해 유의하게 증가하였으나 acyl carnitine은 유의한 차이가 없었다. 결 론 : 지방간이 동반된 비만아에서 장쇄 지방산이 뚜렷이 증가되었으며, 비만아군에서 정상아에 비해 혈청 carnitine이 증가되어 있음을 알 수 있었다. 이것을 기초로 하여 비만아에서 L-carnitine 투여 후 지방산 대사의 변화에 대해 추후에 연구할 예정이다. Purpose : The prevalence of obese children has recently increased. Obesity is known to be associated with complications such as hypertension, fatty liver, hyperlipidemia, and insulin resistance. L-carnitine is an essential cofactor for the transport of long chain fatty acids into mitochondria for ${\beta}$-oxidation. The purpose of this study is to measure serum free fatty acid and carnitine levels, and evaluate the role of L-carnitine as a therapeutic drug in obese children with fatty liver. Methods : Nine obese children, ranging from seven to 18 years of age, and 10 normal children were examined. Serum lipid(total cholesterol, triglyceride, HDL-cholesterol, and LDL-cholesterol) and fatty acid levels were analyzed. Serum total, free, and acyl carnitine levels were performed also by a new enzymatic cycling technique. Results : Long chain fatty acids(myristic acid, palmitoleic acid, palmitic acid, linoleic acid, oleic acid, and stearic acid)were significantly increased in obese children compared to the control group. Total, and acyl carnitine levels were significantly increased in obese children compared to the control group. Conclusion : Serum free fatty acid and carnitine levels were significantly increased in obese children with fatty liver compared to the normal control. This may suggest that L-carnitine can be used as antilipidemic agent to decrease fatty acid and lipid levels for obese children. Prospective studies will investigate serum fatty acid and carnitine levels after treatment of L-carnitine in obese children in the future.

옥수수 분리 엽록체에서의 엽록소 형광의 소멸과 광계 2의 Quantum Yield

이진범,박인호,이혜주,선우양일,Lee, Chin- Bum,Park, In-Ho,Lee, Hae-Joo,Sunwoo, Yang-Il 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6

옥수수 엽육세포의 분리엽록체를 이용하여 환경요인(광 및 온도)에 따른 산소소비량과 방출되는 형광을 측정하여, 형광소멸 요인을 조사하고 이들과 광계 2의 quantum yield와의 관계를 알아보고자 하였다. 고등식물에 있어 틸라코이드 막은 흡수된 빛의 분포 및 소실을 조절하는 수개의 기작을 가지며, 이들은 내외의 여러 요인들에 의하여 조절되어진다(Horton et al., 1989), 'Light doubling techniques' 와 달리 DCMU처리에 의한 방법은 energy-dependent quenching(qE)을 확실히 구별할 수 있는 장점이 있다. 광의 세기가 낮을 때는 photochemical quenching(qQ)이 형광소멸의 주된 요인임을 알 수 있으며, 광의 세기가 높을수록 non-photochemical quenching(qNP)이 주된 요인이었다. qQ와 산소소비량에 대한 quantum yield$({\Phi}_s)$사이에는 nonlinear한 관계를 보였으며, 이는 높은 광에서 광계 2의 quantum yield(intrinsic yield, $({\Phi}_p)$의 감퇴를 나타낸다 하겠다. ${\Phi}_p$는 광도의 증가에 따라 감퇴하였으며, qE와 역비례 관계를 보였으나 ${\Phi}_p$의 감퇴는 qE의 상승에 기인하기 보다는 pH변화 등 다른 요인의 영향일 것이다. 온도상승에 따라 산소소비량은 증가하였으나, qE, qR 및 qNP는 계속적인 감소경향을 보였다. 한편, $(F_v){_m/F_m}$와 ${\Phi}_p$와의 관계는 변화양상이 매우 유사하여 linear관계를 가질 것으로 사료되며, 이와 같은 관계를 가질 경우, 안테나에서의 열손실이 중요한 요인으로 작용할 것이다. The relationship between quantum yield of photosystem II and quenching component of chlorophyll fluorecene has been investigated through a series of experiments on isolated maize mesophyll chloroplasts. The thylakoid membranes of higher plants possess several mechanisms that control both the distribution and the rate of dissipation of absorbed light. These mechanism allow regulation by external and internal factors. By using DCMU addition techniques, there is an advantage to be able to distinguish energy-dependent quenching (qE) from other components of non-photochemical quenching (qNP). At low light intensity, photochemical quenching (qQ) is major component of chlorophyll fluorescence quenching, while at high light intensity, qNP is major one, The relationship between qQ ans quantum yield of oxygen evolution $({\Phi}_s)$ with incident light intensity was showed. The nonlinear relation is indicative of the decline in the intrinsic yield of photosystem II $({\Phi}_p)$ at high light intensity. ${\Phi}_p$ declined as the light intensity increased, which was inversely proportional to qE. The oxygen evolution was increased gradually as the temperature increases, but over $45^{\circ}C$, decreased rapidly. qE, qR (the remaining quenching) and qNP were continuously decreased, while qQ was rapidly increased over $30^{\circ}C$. The changes of $(F_v){_m/F_m}$ on temperature may be presumably able to suggest that they are affected by the phophorylation of LHC II. And interestingly, the changes of $(F_v){_m/F_m}$ were very similiar with those of ${\Phi}_p$.

Silicon Elastomer에 관(關)하여 (기일(其一))

이진범,Lee, Jin-Beom 韓國고무學會 1970 엘라스토머 및 콤포지트 Vol. No.

조강형 라텍스개질 스프레이 몰탈의 역학적 특성

이진범(LEE, Jin, Beom),최성용(Choi, sung yong),김기헌(Kim, Ki, Heun),김용곤(Kim Yong, Kon),윤경구(Yun, Kyong, Ku) 한국콘크리트학회 2006 한국콘크리트학회 학술대회 논문집 Vol.2006 No.11

1㎬급 선조질강의 항복응력 체결에 따른 수소지연파괴 거동분석

이진범(J. B. Lee),강남현(N. H. Kang),박지태(J. T. Park),안순태(S. T. Ahn),박영도(Y. D .Park),조경목(K. M. Cho) 한국소성가공학회 2010 한국소성가공학회 학술대회 논문집 Vol.2010 No.5