해수유통 중인 간척담수호 화성호에서 식물플랑크톤의 군집과 적응전략에 대한 수문학적 영향

송태윤 ( Tae Yoon Song ),유만호 ( Man Ho Yoo ),이인호 ( In Ho Lee ),강의태 ( Eue Tae Kang ),김미옥 ( Mi Ok Kim ),최중기 ( Joong Ki Choi ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.2

A survey was carried out to understand the influence of hydrology on the composition, abundance and adaptive strategies of phytoplankton in artificial Lake Hwaseong, an estuarine reservoir with seawater exchange through a sluice. Samples were collected seven times from May to October 2012. Hydrological events (seawater exchange, rainfall) resulted in a wide variation in salinity along with nutrients and turbidity. Shifts in the dominant phytoplankton composition occurred on every survey. Chlorophyll-a ranged from 9.7 to 104.1 μg L-1. Multivariate analysis allowed us to identify the four phases on phytoplankton community change. Phase I (May~June) was characterized by small-sized Gymnodinium sp. and Heterosigma akashiwo dominated in warm temperature and high salinity derived from seawater exchange, and followed by Cylindrotheca closterium blooms due to rainfall and winds during phase II (July and September). During phase III (August), the dominance of Oscillatoria spp. was correlated with high temperature and low salinity. Abundant cryptomonads were associated with lower temperature during phase IV (October). Adaptive strategies were identified in the phytoplankton as morphological and physiological characteristics. These strategies identified small-sized flagellates as CR-strategists, fast-growing opportunistic species, which might favor the weak stratification of lake due to the seawater exchange during phase I and IV. Dominant species during phase II and III were characterized with R-strategists, medium-sized stress-tolerant species, which might favor turbulence by river flow. The results indicate that stronger stratification following the termination of seawater exchange for the freshening might intensify the predominance of smaller flagellates. In conclusion, this study suggests that hydrology may drive phytoplankton community change and blooms through the controls of salinity, turbulence and nutrients.

진해만 서부해역에서 식물플랑크톤의 시.공간적 분포특성

유만호,송태윤,김은수,최중기,Yoo, Man-Ho,Song, Tae-Yoon,Kim, Eeu-Soo,Choi, Joong-Ki 한국해양학회 2007 바다 Vol.12 No.4

We studied spatial and temporal distributions of the phytoplankton and their relationships to physico-chemical environmental factors in the western Jinhae Bay, Korea from November 2003 to August 2004. In most cases, physico-chemical environmental factors showed homogeneous distribution. The phytoplankton communities were composed of mainly diatoms and dinoflagellates, and their standing crops ranged from $16{\times}10^3\;cells\;l^{-1}\;to\;5,845{\times}10^3\;cells\;l^{-1}$ (with a mean value of $555{\times}10^3\;cells\;l^{-1}$). The bloom of phytoplankton was observed in Gohyun Port in the summer. Seasonal variation of phytoplankton standing crops was higher in winter and summer than in spring and autumn. The dominant species were Skeletonema costatum, Akashiwo sanguinea, Pseudo-nitzschia pungens, Dactyliosolen sp., Leptocylindrus danicus, cryptomonads and etc. Especially, S. costatum was predominant in the summer and A. sanguinea (spring and autumn), Pseudo-nitzschia sp. (summer), Guinardia striata (spring), unidentified flagellates (summer) and cryptomonads (spring) appeared to be an opportunistic species. Concentrations of Chl a ranged from $0.6{\mu}g{\cdot}l^{-1}\;to\;16.7{\mu}g{\cdot}l^{-1}$ (with a mean value of $3.4{\mu}g{\cdot}l^{-1}$). The results of the canonical correspondence analysis implies the study area was grouped into the 2 water masses (inner and outer waters of Gohyun Port) and inner waters had higher abundance and Chl a concentration than outer waters. Also, phytoplankton sanding crops were related with temperature, DO and nutrients ($SiO^2$, TN, TP and etc.) in inner waters. Inner water-mass of Gohyun Port expanded between Gacho Is. and Chilchon Is. during the winter. 남해 진해만 서부해역에서 2003년 11월부터 2004년 8월까지 식물플랑크톤의 시 공간 분포특성 및 해양환경요인과의 상관성을 분석하였다. 대부분의 해양환경요인은 균일한 공간분포를 보였고, 식물플랑크톤 종조성은 돌말류와 와편모류가 주를 이루었으며 개체수는 연간 $16{\times}10^3\;cells\;l^{-1}{\sim}5,845{\times}10^3\;cells\;l^{-1}$(평균 $555{\times}10^3\;cells\;l^{-1})의 범위로 변화하였다. 식물플랑크톤의 대발생은 고현항 내측수역에서 하계에 발생하였다. 계절별 식물플랑크톤 현존량은 2월(동계)과 8월(하계)에 높고, 5월(춘계)과 11월(추계)에 낮았다. 연구기간동안 Skeletonema costatum, Akashiwo sanguinea, Pseudo-nitzschia pungens, Dactyliosolen sp., Leptocylindrus danicus, cryptomonads 등이 식물플랑크톤 우점종으로 출현하였다. 특히, S. costatum은 하계에 극우점하여 출현하였으며, A. sanguinea(추계와 춘계), Pseudo-nitzschia sp.(하계), Guinardia striata(춘계), 미동정 편모류(하계), cryptomonads(춘계) 등은 특정시기에 높은 우점률을 보였다. Chl a 농도는 연간 $0.6{\mu}g{\cdot}l^{-1}{\sim}16.7{\mu}g{\cdot}l^{-1}$(평균 $3.4{\mu}g{\cdot}l^{-1}$)로 나타났다. 정준대응분석 결과 진해만 서부해역은 크게 두 개의 수역(고현항 내측과 외측)으로 나눠지며, 높은 밀도의 식물플랑크톤 현존량과 Chl a 농도를 보인 고현항 내측의 경우 수온, 용존산소, 영양염류($SiO^2$, TN, TP and etc.) 등이 식물플랑크톤 군집에 영향을 미치는 해양환경 요인으로 나타났다. 고현항 내측수괴는 동계에 가조도와 칠천도 사이의 중앙 수역까지 확장되어 나타났다.

Enterococcus faecalsis 유래의 신규 L-aspartate β-decarboxylase의 cloning, 정제 및 활성 규명

이동근,송태윤,김남영,이어진,하상안,이재화,하종명,하배진,이상현,Lee Dong-Geun,Song Tae-Yoon,Kim Nam Young,Lee Eo-Jin,Ha Sang-An,Lee Jae-Hwa,Ha Jong-Myuong,Ha Bae Jin,Lee Sang-Hyeon 한국생명과학회 2006 생명과학회지 Vol.16 No.1

L-alanine의 산업적 생산을 위한 신규의 L-aspartate $\beta-carboxylase$ 유전자를 Enterococcus faecalis에서 검색하고 이를 대장균에 형질전환시켰다. E. faecalis 유래의 ADC유전자 는 1611 bp의 염기서열로 구성되어 있으며 형질전환된 대장균에서 59 KDa의 효소를 생산하며 L-aspartate $\beta-carboxylase$의 촉매활성을 나타내는 것을 확인하였다. 본 연구결과로 기능은 모르고 유전체 서열만 아는 균체에서 신규 효소를 개발하는 방법을 확립하였으며 저가의 aspartate를 이용한 고부가가치 L-alanine을 생산할 수 있는 신규효소를 개발할 수 있었다. The gene for a L-aspartate $\beta-decarboxylase$ (ADC) from Enterococcus faecalis was cloned and sequenced. The gene comprised an open reading frame of 1,611 base pairs, which encodes a protein of 58,960 Da consisting of 536 amino acid residues. The gene was subcloned into an expression plasmid for overexpression of the ADC. The recombinant ADC was produced using E. coli as the host and purified to homogeneity. Our result showed that the ADC may be obtained from bacteria known nucleotide sequence. Thus, we suggest that high value L-alanine might be produced by low value aspartate.

한강하구에서 조석주기에 따른 웅어속 자치어의 출현량 변동

김지혜 ( Ji-hye Kim ),송태윤 ( Tae-yoon Song ),김병기 ( Byung-gi Kim ),김병표 ( Byung-pyo Kim ),한경남 ( Kyung-nam Han ) 한국어류학회 2016 韓國魚類學會誌 Vol.28 No.3

조류에 의한 에너지가 상대적으로 매우 큰 한강 하구역의 대표적인 석모수로와 염하수로에서 2007년 8월과 2008년 8월에 연속 관측을 통해 조석주기에 따른 소하성 어종인 웅어 속 자치어의 출현량을 조사하였다. 조석주기에 따른 환경측정 결과, 1 ℃ 미만의 수온차를 보였고, 염분은 창조 시 최고 15.1 psu, 낙조 시 최저 0.8 psu로 조석에 따른 염분차가 컸다. 웅어속 자치어는 창조 시보다 낙조 시 높은 출현량을 보였다 (p<0.05). 이는 소하성 어종인 웅어속 자치어가 한강 상류 또 는 중류에서 부화한 후, 보육장인 하구역에 정착하기 위해 수평이동 선택적 조수흐름 (STST)으로 낙조류를 이용하는 것으로 사료되며, 특히 낙조 시 13.6mm 이상의 유영력이 향상된 개체일수록 뚜렷하게 나타났다. The Seokmo and Yeomha channels are representative areas of high tidal energy in the Han River estuary. Surveys of environmental variables and abundance of Coilia spp. larvae, an anadromous fish, were carried out following the tidal cycle in August 2007 and August 2008. It was found that mean water temperature varied by <1 ℃ across flood and ebb tides. A clear difference in salinity was observed between tides, with a high of 15.1 psu during flood tide, and a low of 0.8 psu during ebb tide. Coilia spp. larvae were significantly more abundant during the ebb tide than the flood tide (p<0.05). Since Coilia spp. larvae are anadromous fish, it seems that they use the ebb tide as a Selective tidal stream transport (STST) for horizontal movement in order to settle in the estuary (cultivation area), following hatching in the upper/middle courses of the Han River. A high percentage of larvae with improved swimming ability (measuring ≥13.6 mm in length) was observed during the ebb tide.

방조제 건설에 따른 새만금 표층 유기탄소 분포 특성

박준건(Jun Kun Park),김은수(Eun Soo Kim),김경태(Kyung Tae Kim),조성록(Sung Rok Cho),송태윤(Tae Yoon Song),유정규(Jeong Kyu Yoo),김성수(Seong Soo Kim),박용철(Yong Chul Park) 한국해양환경·에너지학회 2009 한국해양환경·에너지학회지 Vol.12 No.2