대두 및 대두발효식품의 항돌연변이성

윤기도,권동진,홍석산,김수일,정건섭 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

To investigate the inhibitory effect of soybean and Korean traditional fermented soybean products on the chemically induced mutagenesis, we extracted soybean, Kanjang, Doenjang, Kochujang, and Chonkukjang with water, methanol and hexane. Inhibitory effect of extracts was assayed by the SOS chromotest using Escherichia coli PQ37 as a test strain. 4-nitroquinoline-1-oxide(4NQO), N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), and aflatoxin B_1(AFB_1) were used as mutagens. Methanol extracts showed relatively higher inhibitory effect than water and hexane extracts. Methanol extracts of soybea, Doenjang, Kochujang, and Chongkukjang showed inhibitory effect of 68.4, 96.3, 17.5, and 100.9%, against MNNG, and 28.6, 109.1, 41.3, and 101.8% against AFB_1., respectively. Doenjang methanol extract showed inhibitory effect of 51.0, 96.3, and 109.1% against 4NQO, MNNG, and AFB_1. Inhibitory effect of heat-treated Doenjang and Chongkukjang methanol extracts on the mutagenicity of MNNG and AFB_1 was remained over 95% of the inhibitory effect of heat-untreated extracts, demonstrating the heat stability of the potent antimutagenic activity.

HPLC-MS/MS를 이용하여 혈장 중 레보티인정의 정량분석

채석,오형근,이상선,김형기,손동렬,염윤기,맹주양,권준택 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2

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핸드스프링 몸펴 앞공중1회 비틀기 동작의 소요시간 및 각운동량 분석

권오석,윤양진,서국웅 한국운동역학회 2002 한국운동역학회지 Vol.12 No.2

남자 기계체조 국가대표 선수 3명과 대학선수 3명을 대상으로 핸드스프링 몸펴 앞공중1회 비틀기 동작을 구간 및 국면별로 분석하고 동작의 숙련도에 따라 역학적 변인이 집단 간 어떠한 차이를 보이는지를 구명하기 위하여 Kwon3D 프로그램을 활용하여 분석한 결과 다음과 같은 결론을 얻었다. 시간요인에서 보폭을 크게 딛는 것이 손을 빠르게 지지 할 수 있고, 핸드스프링 체공시간은 짧게 하여야 발구르기의 압력을 높여 비틀기동작에서 체공 소요 시간이 길어지므로 비틀기 수행의 완성도를 높일 수 있는 것으로 나타났다. 각운동량 요인에서 기술수행 시 전체적으로 좌우(X)축에 대한 각운동량이 전후(Y)축과 수직(Z)축에 대한 각운동량에 비해 더 큰 값을 보였다. 좌우 축 각운동량은 지면에서 이지되어 동작을 수행하는 핸드스프링회전과 앞공중1회 비틀기에서 숙련자가 상대적으로 더 큰 각운동량 보이는 것으로 나타났다. The purpose of this study were a two-fold: ⑴ to analyze event and phase of handspring salto forward stretched with turn; ⑵ to know the differences in the kinematic variables between two groups. A Kwon3D program served for the estimation of this study. The group was divided into three National representative and three well-trained calisthenics in this study. The results of this study revealed that ⑴ the forward somersault performance was increased when duration time in the air was long during the salto forward stretched that the duration time of Handspring is short, the pressure at takeoff is high, the stride is large, and hands are supporting on the ground quickly; ⑵ comparing the angular movement of anterior and posterior y axis and vertical z axis, the angular movement of right and left x axis was higher during the performance. As a result, the national representative players showed better performance in Handspring salto forward stretched with turn.

제지 폐수의 질소·인산 동시 제거를 위한 생물학적 처리기술 개발

권기석,노윤숙 안동대학교 환경연구소 2001 환경연구 Vol.1 No.-

주변에서 원유로 오염된 토양과 난분해성 화합물을 포함하고 있는 폐수 등에서 균주를 분리하여 40여종의 분해균주 중 가장 분해력이 우수한 분해균주 4종을 LB(Luria Bertani)배지로 최종분리하였다. 자연계의 균원 시료로부터 분리된 균주 중 점성을 갖는 균주를 선별하여 그 중 표준 점토광물인 kaolin clay에 대한 응집활성이 뛰어난 34종류이 균주를 선택하였으며, 폐수 내에서 응집활성이 높은 응집균주 4종을 최종 분리하였다. 균주 #46(Bacillus circulans), O24(Pseudomonas sp.)2종을 제외한 나머지 균주들의 동정결과는 #24T3, #24P3, #24B3는 Bacillus sp.로 동정되었고 #81, #152, 29은 미동정 되었다. 분해균주의 경우 TOC가 84%, T-N이 98%, T-P가 68%의 처리효율을 보였다. 응집균주의 경우 TOC가 80%, T-N 98%, T-P가 71%의 처리효율을 보였다. 분해+응집균주가 microcosom을 형성한 경우 TOC가 85%, T-N의 경우 98%이상, T-P의 경우 70%을 동시에 처리할 수 있다. From the enrichment culture, 40 strains were isolated, Among them, four strains were screend as having significant biodegradation activity of crude oil and toxic compounds containing wastewater. Also, for the screening of the microorganims, 34 bacterial strains were selected. Among them, four strains were screened as producing the most excellent flocculating material. The strains were identified that 46 was Bacillus circulates, 024 was Pseudomonas sp., 24T3, 24B3, 24P3 were identified Bacillus sp., respectively, And 81, 151, 29 were non-identification. The optimum cultural conditions were as follows; Bioflocculant producing strains : Glucose 20.0g, NH_(4)NO_(3) 2.0g, K_(2)HPO_(4) 0.8g, KH_(2)PO_(4) 0.6g, MgSO_(4)·7H_(2)O 0.05g, MnSO_(4)·4H_(2)O 0.05g, Soytone 0.lg, Tryptone 0.lg Yeast extract 0.lg, CaCO_(3) 0.3g in 1 liter of distilled water, 30℃ for temperature and pH 7.0. Biodegrade strains : LB(Luria Bertani) medium, 30℃ for temperature and pH 6.8∼7.5. The removal rates of total nitrogen and total phosphate by bioflocculant producing strains against pulp wastewater were T-N 98% and T-P 71%, respectively. In the case of biodegrade strains shown that the removal rates were T-N 98% and T-P 68%, respectively. In the case of mixed biodegrade strains and bioflocculant producing strains shown that the removal rates were T-N 99% and T-P 76%, respectively. The strains will be apply simultaneously T-N 98% and T-P 70% from pulp waserwater.

후방복사된 초음파를 이용한 12Cr강 부식 피로특성 평가

권성덕,윤석수,송성진,배동호 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.5

화력발전 플랜트의 터빈 블레이드(blade) 재료인 12Cr 합금강의 고속 열화 시편에 대한 레일리(Rayleigh) 표면탄성파의 산란과 속도의 주파수 의존특성을 액체/고체 경계 면에서 표면파의 발생기구에 의해 나타나는 후방복사 초음파 세기의 입사각 의존성으로 간접 평가하고 부식피로 특성과 비교 분석하였다. 후방복사 현상이 발생하는 입사각 폭은 부식온도와 반비례하였으며 이는 효과적 부식층의 두께 증가로 설명되었다. 이 프로파일의 폭은 피로시험에서 균열성장 관계식인 파리스(Paris) 법칙의 지수 m과 선형적으로 반비례하여 열화시편의 부식 피로 특성의 비파괴적 평가에 있어 후방복사된 초음파를 이용한 기술이 매우 유용함을 보여주었다. The corrosion-fatigue characteristics of the 12Cr steel, which is widely used in fossil power plants as a turbine blade material, are evaluated nondestructively by use of the Rayleigh surface wave. In this study, the frequency dependency of the Rayleigh surface wave is investigated indirectly by measuring the angular dependency of the backward radiation of the incident ultrasonic wave in the aged specimens, and then compared to the corrosion-fatigue characteristics. The width of the backward radiation profile decreases as the increase of the aging temperature, which seems to result from the increase of the effective degrading layer thickness. This parameter also shows an inversely proportionality to the exponent, m, in the Paris law which predicts the crack size increasement due to fatigue. The result observed in this study demonstrates high potential of the backward radiated ultrasound as a tool for the nondestructive evaluation of the corrosion-fatigue characteristics of the aged materials.

하수슬러지를 이용한 복합소재 개발연구

연규석,권윤환,유근우,김남길,김동준,이윤수 강원대학교 부설 석재복합신소재 제품연구센터 2002 석재연 논문집 Vol.7 No.-

본 연구는 하수 종말 처리에 의해 발생되는 하수 슬러지를 잔 골재(경량골재)로 사용하여, 폴리머 콘크리트를 제조하여 그 때의 역학적 성질에 대하여 검토하였다. 하수 슬러지는 5일간 110±5℃에서 수분함량 0.1%미만으로 건조한 후 파쇄하여 No.4∼No. 200 번체의 잔골재 표준 시방서 기준에 부합되도록 사용하였다. Pellet는 보통 회전pan에서 만드나, 본 실험에서는 수동으로 제조하였다. 제조한 하수 슬러지 경량 골재는 평균 겉보기 비중은 1.58(g/㎤), 단위용적중량은 1.98(t/㎥)으로 KS F 2534의 경량골재의 절건비중과 단위용적에 따른 분류에 의하면 중위급(medium weight) 경량골재에 속하는 것으로 측정되었다. 또한 잔 골재 중 하수 슬러지 경량 골재를 0%, 5%, 10%, 15%, 20%, 25%, 30%로 치환하여 그때의 압축, 휨, 인장강도를 측정하였다. 그 결과 하수 슬러지의 증가에 따라 강도 감소를 보였으나, 일반 경량콘크리트 제조 또는 단열용 콘크리트 블록 제조에 사용이 가능하다고 사료된다. 그러므로 앞으로 건조수축, 크리이프 특성 및 내구성에 관한 연구가 더욱 심도 있게 이루어져야 폐자원인 하수 슬러지를 이용한 건자재의 개발이 가능하리라 판단된다. In this study, the polymer concrete was produced by using the lightweight aggregate that had been manufactured using the sewage sludge and its physical properties were experimentally examined. Experimental results show that when the portion of sewage sludge lightweight aggregate in the fine aggregate increases, slump values decreases. Also, the compressive, the flexural and the tensile strengths decrease when the portion of sewage sludge lightweight aggregate increases. Although its strengths decreases, the sewage sludge lightweight fine aggregates is thought to be used for construction materials.

忠州市 地域特性을 反暎한 共同住宅 計劃硏究 : 충주지역 거주자의 APT 선호특성을 중심으로

朴碩鉉,朴義權,柳顯紀,尹勝照,孫泰鎭,金基洙 충주대 2003 産業科學論文集 Vol.11 No.-

The purpose of this research is to analyze the correlation of the characteristics of dwellers and residential preference in apartment housing through the case study of 340 samples in 25 housing estates which were built in Chung-ju city The contents of this research consist of two main parts. The first part is to identify characteristics of dwellers, which might have an influence on residential preference in apartment housing. The second part is to identify the main physical elements of residential preference that can be influenced by the personal characteristics. Thus, this research will contribute to the establishment of the planning methods which increase residential preference in apartment housing, while promoting the quality of residential environment in Chung-ju city.

한국인에서의 CYP2C19 돌연변이 유전자형의 다형성에 관한 연구

채석,채상우,양동규,김형기,권준택,염윤기 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The S-mephenytoin 4'-hydroxylase (CYP2C19) metabolizes a number of clinically used drugs and shows a marked interethnic difference in the incidence of the poor metabolizer (PM). Recent studies have found a third (CYP2C19*4) and forth (CYP2C19*5) mutations in Caucasians. In the present study, we genotyped 536 healthy unrelated Koreans for functionally defective alleles, CYP2C19*2, CYP2C19*3, CYP2C19*4, and CYP2C19*5. Detection of the normal (CYP2C19*1) and defective alleles was performed by polymerase chain reaction/restriction enzyme analysis. The genomic DNA was isolated from peripheral blood. The allelic frequencies of the wild-type (CYP2C19*1) and CYP2C19*2 were 72.6% and 27.4%, and the wild-type (CYP2C19*1) and CYP2C19*3 were 85.1% and 14.9%, respectively. For each CYP2C19*2 and CYP2C19*3 the observed number of three genotypes were similar to those calculated in accordance with the Hardy-Weinberg equation. The frequencies of homozygotes for CYP2C19*2, CYP2C19*3 and compound heterozygotes were 9.7%, 2.8% and 4.2%, respectively. The mutants of CYP2C19 were identified in 36 subjects (16.7%). The mutated CYP2C19*4 and CYP2C19*5 alleles were not detected in this study. These results suggest that frequency of mutants of CYP2C19 in Koreans resembled the Orientals rather than Caucasians, and we founded the racial variability of the frequencies of the CYP2C19*4 and CYP2C19*5 alleles in the Orientals.

장기입원환자 관리 방해요인

이경석,윤석만,권희,이호,김혜경,조미자,한규남,김명성 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background: Recently the health insurance review agency tried to cut off the cost of unnecessary hospital stay. It is important to reduce the prolonged occupation of the hospital beds. However, there are some obstacles of the management for the prolonged bed occupation. We report our experiences and the problems of bed-control. Methods: To increase the rate of effective bed-usage, a task force was made. This team was consisted of 4 dctors, 2 nurses and 2 personnel of the administrative department. From December 20th 2001 to May 17th 2002, the task force analysed the rate, number and causes of the prolonged (more than 60 days, regardless of the disease entities) bed occupation. We announced and explained the necessity of bed-control and actively pressed to reduce the prolonged bed occupation. Results: The number of the prolonged bed occupation was 71 at the beginning of the management. It was reduced to 46 at best, then turned back to 65 at the end. It seems to be impossible to reduce the prolonged bed occupation until there are sufficient numbers of long-term care facilities. Aggressive bed control makes the sick patients to move every two or three months, it is never desirable. Conclusion: We cannot expect any Quality Improvements without improvement of the wrong system. Prior to the management for the prolonged bed occupation, it is necessary to build a sufficient number of long-term care facilities.

사망 진단서(시체 검안서) 작성의 문제점

김규석,임용수,이중의,서길준,윤여규,어은경,염석란,정연권,이윤성 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: This study was conducted to analyze the current problems in completing death certificates and to identify the correct method for completing death certificates Methods: We reviewed 262 death certificates in three hospitals from March 1 to April 30, 2000, and 119 death certificates in one hospital from March 1 to 31, 2000. We identified major and minor errors and analyzed and compared them retrospectively. Results: A total of 381 death certificates were reviewed: 59 in Seoul National University Hospital,101 in Ewha Woman's University Hospital, and 102 in Gachon Medical College Hospital, which has no education program for completing death certificates in postgraduate training, and 119 in Samsung Medical Center which has an education program for completing death certificates. 358 certificates(94.0%) had at least one error. There were only 23 death certificates(6.0%) without an error. In 182 cases(47.8%), there was one major error. In 321 death certificates(84.3%), there were more than two errors. A comparison of Samsung Medical Center with the other hospitals showed that the number of total errors was statistically different(p=0.001). Conclusion: There were few death certificates without an error in this study. In a hospital which has postgraduate training in completing death certificates, there are fewer errors than in other hospitals which have no training course. Emergency physicians actually certify many deaths, so they must know the correct method of completing death certificates for statistics on morbidity and mortality.