제주연안 갯녹음(백화) 지역의 해수에 분포하는 세균군의 분자생물학적 분석

강봉조,김미란,윤병준,이동헌,오덕철,강형일 濟州大學校 基礎科學硏究所 2002 基礎科學硏究 Vol.15 No.2

본 연구에서는 갯녹음(백화)현상이 세균생태계와 어떤 관련이 있는지에 대한 기초자료 및 정보를 얻기 위하여 갯녹음 현상이 일어난 제주도 성산과 강정지역 연안의 해수에 존재하는 세균군을 16S rRNA 증폭기법을 이용하여 조사하였다. 강정지역에서는 Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacter, Sulfitobacter, Roseobacter 등 다양한 종류의 세균이 분포되어 있었으며, Alcanivorax가 20%의 빈도로 가장 많이 나타났다. 반면, 성산 지역에서는 Pseudomonas속 균주가 우점종으로서 존재하였으며, Pseudomonas tolaasii(혹은 Pseudomonas corrugata)와 유연관계가 가까운 세균은 44%, Pseudomonas mandeli와 가까운 세균이 24%, Verrucomicrobiales와 가까운 세균은 4%, 기타 동정되지 않은 세균은 세 group으로 구분되었으며, 각각 8%, 8%, 12%를 차지하여, 두 곳에 분포되어 있는 세균군상이 상당한 차이점이 있음을 확인하였다. 갯녹음 지역인 강정과 성산 해수의 8월(표품 채집시기)의 수온은 27℃-27.5℃, 염분의 농도는 30.24-30.60%, pH는 8.23-8.36, 용존산소량(DO)은 각각 7.20-7.28로, 두 지역에서 매우 비슷한 것으로 조사된 바 있는데, 이는 수온이나 염분의 농도, 또는 pH보다는 다른 원인에 의하여 두 갯녹음 지역에 분포하는 세균군의 차이를 가져왔음을 제시해 주었다. In this study, the bacterial communities distributed in sea water of the whitening areas of Gangjeong and Seongsan, Jeju-do have been analyzed using the PCR amplification of 16S rRNA to obtain fundamental data and information on relationship of the whitening phenomenon and microbial ecosystem. In Gangjeong, diverse bacteria such as Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacier, Sulfitobacter, and Roseobacier have been found, and Alcanivorax was the most abundant clone. The most abundant clone from Seongsan was Pseudoinonas, of which Pseudomonastolaasii and Pseudonionas mandeli were most abundantly occurred in the frequency of approx 44% and 24%, respectively. Approx 4% of the bacterial clones closest to firruconiicrobiales and other unidentified clones were also found in Seongsan, suggesting there is a great discrepancy between bacterial communities from the whitening areas of Seongsan and Gangjeong. The mean tem- perature, chlorine concentration, pH, and dissolved oxygen (DO) of the sea water of Gangjeong and Seongsan in August of 2001 (sampling period) was 27℃-27.5℃, 30.24-30.60%, pH 8.23-8.36,7.20-7.28 ㎎/ℓ, suggesting other environmental factors except for the factors mentioned above might result in difference of bacterial communities distributed in both areas.

급성 췌장염을 동반한 임신성 급성 지방간 1 예

강미선;박상훈;박지영 서희영;허란;김상현 인제대학교 백병원 2011 仁濟醫學 Vol.32 No.-

Acute fatty liver of pregnancy (AFLP) was first described as a specific clinical entity in 1940 and thought to be universally fatal. Maternal mortality in the past approached 75 percent. However early diagnosis and prompt delivery have dramatically improved the prognosis, and maternal mortality rate is now less than 18 percent. A early diagnosis, a prompt delivery, and a intensive supportive care have improved maternal and perinatal outcome. We report a 30—year—old woman at 37 weeks gestation who have multiple complications including acute pancreatitis in AFLP. She was complicated with acute renal failure, disseminated intravascular coagulation, duodenal ulcer bleeding, spontaneous bacterial peritonitis, pulmonary edema and acute pancreatitis.

兩立하는 寫傷에서의 共通不動點

金美蘭,姜信玟 慶尙大學校 1991 論文集 Vol.30 No.2

本 論文에서는 漸近正規寫像의 性質을 이용하여 兩立하는 寫傷에 대한 共通不動點定理를 구한 바 그 結果는 다음과 같다. 定理. A, B, S와 T는 完備距離空間(X, d)에서 그 자신으로의 寫傷으로써 다음 條件을 滿足한다고 하자. (1) A(X) ⊂ T(X), B(X) ⊂ S(X) (2) d(Ax, Bv) ≤ ø(d(Ax, Sx), d(Sx, Ty), d(By, Sx), d(Sx, Ty)) (단, ø는 未感少이고 上一半連續인 兩數) (3) A, B, S 와 T 中의 하는는 連續이다. (4) 變 A, B 와 S, T는 兩立한다. (5) 變 (A, B)는 S 와 T 에 變하여 한 點에서 漸近正規이다, 이때, A, B, S와 T 는 唯一한 共通不動點을 갖는다.

학동기 손자녀에 대한 조모의 역할수행 : 취업모가족과 비취업모가족 비교

이미숙,조병은,강란혜 대한가정학회 2004 Family and Environment Research Vol.42 No.4

The purpose of this study was to compare the grandmothers role performance toward their grandchildren in the elementary school between working and nonworking mother families. The subjects were 274 grandparent-grandchild pairs. The sbuctured questionnaires, from both grandmothers and grandchildren were collected in 2001. Using statistical technique such as factor analysis, t-test, Pearson's correlation, and multiple regression analysis, the major findings were as follows: 1) The level of the grandmothers' role performance is moderate in general. However, the grandmothers in working mother families perform surrogate mother roles more strongly than the grandmothers in nonworking mother families. 2) While contact between grandmother and grandchild is associated with the grandmother's role performance in working mother families, the grandmother's age and the closeness between grandmother and mother are related to the level of the grandmother's role performance in nonworking mother families. In conclusion, the grandmothers' role performance in working mother families was found to be more important in the supporting system than in nonworking mother families.

일부 부인과 양성 질환자의 지방조직 및 혈청의 PCBs와 DDE 농도

박성균,이강숙,노영만,구정완,민선영,한진구,고원경,김미란,정재근,이종승,김진홍 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 본 연구는 지방조직과 혈청의 PCBs와 DDE 농도를 측정하고, 이에 영향을 미치는 것으로 알려진 요인들과의 관련성을 평가하고자 하였다. 방법 : 유방암, 자궁내막증 등 유기염소계 화합물의 영향이 있는 것으로 알려진 호르몬 관련 질병을 제외한 부인과 환자 52명을 대상으로 지방조직과 혈청을 분석하였으며, 설문조사를 통하여 연령, 교육수준, 직업, 비만도, 흡연과 음주, 출산력, 수유력등을 조사하였다. 지방조직과 혈청의 PCBs와 DDE 농도는 가스크로마토그래피를 사용하여 측정하였다. 결과 : 지방조직의 경우, 13개 PCBs congeners 농도의 합은 중앙값이 48.29 ng/g, 기하평균값이 56.78 ng/g이었고, DDE 농도는 중앙값이 142.89ng/g, 기하평균값이 117.06 ng/g이었으며, 혈청의 경우는 PCBs congeners 농도의 합은 중앙값이 4.67 ㎍/L, 기하평균값이 4.85 ㎍/L이었고, DDE 농도는 중앙값이 1.75 ㎍/L, 기하평균값이 2.09 ㎍/L로 나타났다. 지방조직과 혈청의 DDE 농도 사이는 높은 상관성을 보였으나(r²=0.310, p=0,0002), PCBs는 상관성이 약한 것으로 나타났다(r²=0.029, p=0.2582). 영향요인을 범주화한 후 로그변환 시킨 지방조직과 혈청의 PCBs, DDE 평균의 차이를 분석한 결과, 지방조직의 DDE만이 출산자녀수(p=0.015), 초산연령 (p=0.014), 비만지수(p=0.035)와 연관성이 발견되었다 다중회귀분석의 결과 지방조직의 DDE는 출산자녀수가 적을수록, 초산연령이 늦을수록 증가하는 경향을 보였다. 결론 : 출산자녀수와 초산연령, 비만도가 지방조직의 DDE와 관련성을 보였으며, 또한 유기염소계 화합물의 장기간의 노출에 의한 영향을 평가하는데 있어서 혈청보다 지방조직이 더 유용한 생물지표임을 알 수 있었다. Objectives : This study was purposed to determine the levels of PCBs and DDE in adipose tissue and serum and to evaluate the relations with factors affecting these levels. Methods : We analyzed adipose tissues and sera from 52 gynecologic benign disease patients aged 27-78 years, except hormonal diseases such as breast cancer and endometriosis. We also surveyed age, education, occupation, body mass index (BMI), smoking, drinking, number of children, and duration of breastfeeding by questionnaires. Tissue and serum levels of PCBs and DDE were measured using gas chromatography. Results : The median and geometric mean levels of PCBs were 48.29 ng/g, 56.78 ng/g for adipose tissue and 4.67 ㎍/L, 4.85 ㎍/L for serum, and those of DDE were 142.89 ng/g, 117.06 ng/g for adipose tissue and 1.75 ㎍/L, 2.09 ㎍/L for serum, respectively. Adipose tissue and serum levels for DDE showed high correlation (r²=0.310, p=0.0002), but those of PCBs didn't (r²=0.029, p=0.2582). In analyses of the differences of the means of log transformed adipose tissue and serum PCBs and DDE levels for groups of potential covariates, only adipose tissue DDE levels were significantly associated with number of children (p=0.015), age at first birth (p=0.074) and BMI (p=0.035). In multiple regression analysis, adipose tissue DDE levels were significantly increased with decreasing number of children and increasing age at first birth. Conclusions : This study suggested that parity and adiposity were associated with levels of DDE in adipose tissue, and adipose tissue is a better biomarker than serum for evaluating the long-term exposure of organochlorines.

韓國型 Turnip Mosaic Virus의 純化

김병훈,권오병,마병철,강미란,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Chinese cabbage(Brassica campestris subsp pekinensis) useful for making a traditional Korean food, Kimchi, has been considered as the most important vegetable in Korea. In order to supply large quantity of chinese cabbage to the consumer, the mass cultivation is demanded. However, the mass production has been faced to serious problem due to fungal, bacterial and/or viral infections. It has been reported that the viral infections are the worst phatogeruc causes in a number of plants. In the chinese cabbage, turnip mosaic virus(TuMV) is the most serious pathogen. Five different strains of TuMV(Cl, C2, C3, C4, and C5) were identified in the world. We are reporting two strains(C4, C5) purified from Brassica nigra. They are all filamentous on electron micrographs. The TuMV C4 is about 1,300nm long, whereas the C5 is about 1,200nm long in average. They are larger than those reported previously(680-900nm). They will be used to produce monoclonal antibodies against the TuMV coat proteins. The antibodies arc required to develop a sensitive and specific screening methods for the TuMV infected plants.

Gastric Calcifying Fibrous Tumor : A Rare Cause of Gastric Subepithelial Lesion

박상훈;오태훈;김병규;강미선;허란;김정연 인제대학교 2011 仁濟醫學 Vol.32 No.-

석회화 섬유종양은 원인 미상의 흔하지 않은 양성 중앙이다. 이 종양은 복강이나 복막에서 기원하는 경우가 많으나 위에서 기원한 석회화 섬유종양은 매우 드물 다. 건강검진시 위내시경에서 우연히 발견된 위 점막하 종양 평가를 위해 28세 여성이 본원으로 전원되었다. 본원에서 시행한 위 내시경에서 위 중간체부에 1.2cm의 평탄한 표면의 위 점막하 종양이 관찰되었다. 초음파 내시경을 시행하였고 점막하층에 위치한 불균질한 저에코성의 병변과 함께 종양 내부에는 음향 음영을 동반한 다수의 고 에코성의 부위가 관찰되었다. 위 점막하 중앙은 악성종양을 완전히 배제할 수 없기 때문에 내시경적 점막하 절제술을 시행하였다. 조직 검사결과 종양은 석회화률 동반하는 호산구성 콜라겐 섬유가 풍부한 위 석회화 섬유 종양으로 진단되었다. 이에 위 점막하 중앙에 관한 다른 문헌을 고찰하면서 본 증례를 보고하는 바이다.

수술로 호전되지 않은 췌장 농양을 내시경 치료로 완치한 1 예

서희영;전태주;김선영;강미선;허란;박지영;서동대;오태훈 인제대학교 백병원 2011 仁濟醫學 Vol.32 No.-

Necrotizing pancreatitis develops in about 10-20% of patients with acute pancreatitis, and pancreatic abscesses develop in 3% of them. The mortality of pancreatic abscess has been reported up to 40% in one study. Recently, there are several reports that show good results with n on-surgical therapy, such as percutaneous drainage and endoscopic drainage, but if the non-surgical drainage fails, surgical therapy are recommended. However, there is no definite therapeutic method which is suggested yet so far after the failure of the surgical drainage. So we report the case in which we experienced successful treatment of pancreatic abscess by endoscopic removal of necrotic tissue through the gastrocystostomy, after the failure of the surgical debridement and internal drainage through gastrocystostomy.

Mito-Tempo Can Protect Against the Allergic Airway Inflammation through Reducing Mitochondrial ROS Generation

( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Mi Ran Kang ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Oxidative stress is well known to be implicated in the development of asthma. The mitochondrial respiratory chain is a major site of intracellular reactive oxygen species (ROS) generation and, at the same time, an important target for the damaging effects of ROS. Mito-Tempo is a specific mitochondrial ROS inhibitor and it is known to be associated with opening of mi-tochondrial permeability transition pore and inhibition of cell necroptosis or apoptosis. However, there is little information on the protective effects of Mito-Tempo on the inflammatory airway disorders including bronchial asthma and its acute exacerbation. We investigate the effects of Mito-tempo on the allergic airway inflammation and hyperresponsiveness using the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice). The OVALPS-OVA mice showed the typical features of neutrophilic asthma; increased airway inflammatory cells, the pathologic changes, the increased levels of Th2 cytokines in lungs of OVALPS-OVA mice, increased mitochondrial ROS generation, and increased bronchial hyperresponsiveness. Interestingly, we found that in OVALPS-OVA mice, Mito-Tempo, a novel mitochondrial targeting agent significantly reduced the increases in inflammatory cytokines, mitochondrial ROS generation, airway inflammation, and bron-chial hyperresponsiveness. These findings indicate that mitochondrial dysfunction including oxidative damage may be im-plicated in the pathogenesis of bronchial asthma and provide the therapeutic potential of a mitochondrial targeting agent, Mito-Tempo, for bronchial asthma.

Bioactive Functional Water Determined on Cell Fate

Mi-Ran Ki,Eun-Joo Lee,Kyung-Ku Kang,Eun-Mi Lee,Myung-Jin Chung,Ji-Yoon Son,Sul-Gi Jeon,Sun Young Park,Kyu-Shik Jeong 대한지질학회 2018 대한지질학회 학술대회 Vol.2018 No.10