흰쥐에서 탄수화물 종류가 운동시 당 및 지질대사에 미치는 영향

권태동,전중기,민경선,김형렬,장응찬 대구대학교 기초과학연구소 1998 基礎科學硏究 Vol.15 No.2

The purpose of this study was to investigate the effects of glucose, sucrose, and glucose polymer(GP) ingestion on the carbohydrate and lipid metabolism during treadmill running in rats. Rats were randomly assigned to normal, control and carbohydrate groups. Carbohydrate group was divided into glucose, sucrose and GP ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either normal saline(control) or carbohydrates solutions of formulated glucose(6%), sucrose(6%) and GP(6%) via polyethlene tube to stomach before treadmill run. Exercise was loaded by a treadmill run at the speed of 1 km/hour for 30, 60 and 120 minutes. The results of experiment were as follows: Plasma glucose levels were decreased in proportion to the exercise time in control, but increased at treadmill run 30 minutes and then gradually decreased with the lapse of the exercise time in carbohydrates compared with normal rats. It decreased in control, glucose, sucrose and GP compared with normal rats in decreasing order at treadmill run 120 minutes(% change of plasma glucose level of control, glucose, sucrose and GP compared with normal rats were 67, 74, 87 and 91 in plasma glucose level at treadmill run 120 min., respectively). Plasma lactate levels were increased by the exercise loading compared with normal in control and carbohydrates ingested rats. It increased in control, sucrose and GP in increasing order compared with normal rats, at treadmill run 120 minutes(% change of plasma lactate level of control, glucose, sucrose and GP compared with normal rats were 167, 185, 149 and 114 in plasma lactate level at treadmill run 120 min., respectively). Plasma FFA levels had tendency to increase by the exercise loading compared with normal in control and carbohydrates ingested rats. It showed increase in the sucrose, glucose, GP and control rats in Increasing order compared with normal rats at treadmill run 120 minutes(% change of plasma FFA level of control, glucose, sucrose and GP compared with normal rats were 151, 153, 166 and 152 in plasma FFA level at treadmill run 120 min., respectively). Plasma acetoacetate levels were higher in control compared with carbohydrates ingested rats at treadmill run 30, 60 and 120 minutes. Plasma beta-hydroxybutyrate levels increased by exercise compared with normal rats in control and carbohydrates ingested rats at treadmill run 30, 60, 120 minutes. Plasma insulin levels were decreased in proportion to the exercise time in control compared with normal rats, but in carbohydrates ingested rats increased at treadmill run 30 minutes and then gradually deceased with the lapse of the exercise time compared with normal. In carbohydrates ingested rats, plasma insulin levies were higher than control. Hematocrit did not show any difference between control and carbohydrates rats at treadmill run 30, 60 and 120 minutes. From these experimental results, it is suggested that the exhaustion of skeletal muscle glycogen, the hypoglycemia, the overproduction of lactate and dehydration is delayed in carbohydrates ingested rats. GP of the carbohydrates is considered as the most effective for these effects. Therefore, intake of carbohydrates before exercise, especially GP seems to increase performance in the prolonged moderate exercise.

기계적 자극을 이용한 인체 중간엽줄기세포로부터 유사-섬유모세포로의 분화 유도

최경민 ( Kyung Min Choi ),서영권 ( Young Kwon Seo ),윤희훈 ( Hee Hoon Yoon ),권순용 ( Soon Yong Kwon ),이화성 ( Hwa Sung Lee ),박용순 ( Yong Soon Park ),손영숙 ( Young Sook Son ),송계용 ( Kye Yong Song ),김영진 ( Young Jin Kim 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4

Recently, in vitro reconstruction of anterior cruciate ligament(ACL) has been tried using tissue engineering technique, especially mesenchymal stem cell(MSC) is considered as a good cell source. In this study, we tried to find the effect of mechanical stimulus on the differentiation of bone marrow-derived MSC into ACL fibroblastlike cell using a cell training bioreactor imposing cyclic mechanical tension whose parameters were 120 min/day, 1 Hz, and 5% strain. After 2 weeks of culture, MSCs under cyclic tension showed more regularly oriented alignment and lower expression of CD73 and CD105 than control by microscopy and FACS analysis. In transcriptional level, type I collagen, type II collagen, fibronectin, elastin, α-smooth muscle actin mRNA expressions of MSCs under cyclic tension were higher than control and similar to native ACL. In conclusion, it is thought that mechanical cyclic tension induced the loss of stemness of MSC, therefore improved the differentiation into ACL fibroblast-like cell.

Pulse Oximetry and Skin Temperature Gradient as Diagnostic Tools of Successful Caudal Block

Duk Kyung Kim,Kyoung Min Lee,Won Kyoung Kwon,Chung Sik Oh,Sung Whan Jang 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.53 No.6

Background: Though caudal block is a relative simple technique, it has not been widely used in adults because of a high failure rate. We assumed that any tests to quantify the changes of sympathetic tone in the affected areas would be excellent indicators of successful block. We tested the usefulness of two candidates (pulse oximetry plethysmographic waveform amplitude measured at 5th toe and calf minus 5th toe skin temperature gradient) as indicators of successful caudal block. Methods: In 45 adult patients undergoing anal surgery with caudal block, these two variables were simultaneously measured at 2-min intervals for 20 min. A two-fold increase in the plethysmographic waveform amplitude from baseline and skin temperature gradient of 0°C were predefined as test criteria of successful block. Results: While the sensitivity, specificity, positive predictive value, and negative predictive value of the skin temperature gradient test were 45.9%, 100%, 100%, and 9.1%, those of the plethysmographic waveform test were 86.5%, 100%, 100%, and 28.6%. The plethysmographic waveform test showed a significantly higher discriminative capacity than the skin temperature gradient test (94.9% vs. 48.7%, P < 0.05) Conclusions: Unlike the skin temperature gradient test, the plethysmographic waveform test showed a considerably high validity in detecting successful block. Considering its simple and real time monitoring potentials together with a high failure rate of caudal block in adults, we cautiously recommend it as a supplemental diagnostic tool to predict successful block, especially when verbal communication with patient is difficult. (Korean J Anesthesiol 2007; 53: S 19∼25)

Effects of Mechanical Stimulation on the Proliferation of Bone Marrow-derived Human Mesenchymal Stem Cells

Choi, Kyung-Min,Seo, Young-Kwon,Yoon, Hee-Hoon,Song, Kye-Yong,Kwon, Soon-Yong,Lee, Hwa-Sung,Park, Jung-Keug Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.6

To support and enhance the in vitro growth and activity of mesenchymal stem cells (MSCs), the cell culture medium may be supplemented with various proteins and factors to mimic the physiological environment in which the cells optimally proliferate and differentiate. In this study, the effects of mechanical factors on cellular metabolic responses were investigated experimentally using a bioreactor. The effects of various chemical factors, such as growth factors, cytokines, and hormones, were also investigated. Based on previous reports demonstrating the important roles of mechanical factors in the growth and activity of MSCs, we sought to evaluate the effects of mechanical stimuli on the proliferation of bone marrow-derived MSCs using a cell training bioreactor that imposed cyclic mechanical stretch, with parameters of 240 min/day, 0.03 Hz, and $5{\sim}15%$ strain. The application of cyclic stretch $(5{\sim}15%\;strain)$ to the MSCs enhanced their proliferation during the early stage(3 days), but not the late stage (14 days), of batch culture. Mechanical stretch did not increase the release of lactate dehydrogenase (LDH) from the MSCs during culture. Appropriate levels of mechanical stretch $(5{\sim}10%\;strain)$ increased collagen synthesis, but did not alter MSC stretch was able to serve as a potent positive modulator of MSC proliferation during the initial stages of culture.

향류마치스 효과를 갖는 새로운 히스톤 Hl 단백질 (p961)의 횐쥐와 토끼에 대한 약물동태

우수경(Su Kyung Woo),윤민혁(Min Hyuk Yun),이재홍(Jae Heung Lee),권광일(Kwang Il Kwon) 대한약학회 2001 약학회지 Vol.45 No.4

A purified histone Hl protein, p961, which plays a role in mediating the condensation of DNA into chromatin, was recently proved as an arthritis-suppressing agent in the mouse CIA model. The pearmacokinetics of p961 was carried out in rats and rabbits. The rat's blood, bile and urine samples were semially collected from the femoral vein, common bile duct, and bladder respective1y; after bolus I.v. injection at low (10 mghg) and high (30 mg/mg) doses. The rabbit's blood samples were also collected from the marginal ear vein after bolus I.Y. injection at a dose 10 mg/kg. P961 and its major metabolite in the physiological samples were analyzed by reverse-phase HPLC using a Vydac C4 protein column and a multistep water-acetonitrile gradient containing 0.24% trifluoroacetic acid. The major pharmacokinetic parameters (AUC, Cmax, MRT t1/2, Vss and Cl) were estimated from the time course of plasma p961 and metabolite concentrations using WinNonlin. A two- compartment model was chosen for p961 as the most appropriate pharmacokinetic model. After I.v, injection of p961 at doses of 10 mghg and 30 mg/kg, more than 80% of p961 was removed rapidly from the plasma within 15 min. The plasma half-life of p961 in rats and rabbits was found not to exceed 12 min. p961 (22448.9 mot wt) was rapidly cleaved to 21612 mol wt fragment and the breakdown product appeared rapidly in the circulation with no lag phase. P961 and metabolite were not detected in rat urine and bile.

Web 기반의 이미지 마킹시스템 환경 구축

권명광,원유헌,장성민,송경남 弘益大學校 2001 미술디자인 논문집 Vol.- No.6

In the course of Internet proliferation, many World Wide Web-related technologies are examined for possible growth and evolution. The use of World Wide Web-based technologies has further fuelled the demand for World Wide Web-based applications. In this paper, We describe the design and implementation of image marking system that help user manipulate their own image. The user can edit, print and merge their own image with other offered image using World Wide Web. Also the user don't need to buy a required software at a high price but only use World Wide Web that offer the service.

운동시 체내 글리코겐 저장상태에 따른 혈장 에피네프린과 젖산 농도의 변화

민경선,김형렬,권태동 대구대학교 인문과학 예술문화연구소 1998 人文科學硏究 Vol.17 No.-

The purpose of this study was to investigate changes of plasma epinephrine and lactate during maximal exercise in various glycogen conditions. The subjects were composed of 7 male college students. They performed the incremental exercise test by way of bicycle ergometer in normal glycogen condition, glycogen-depleted condition and glycogen-loaded condition. Glycogen depletion and loading were formed as a results of exercise and dietary. Plasma epinephrine and lactate were analyzed from blood samples extracted from the antecubical vein every 3 minutes until exhaution. On the basis of the results analyzed in this study, concluions were drawn as follows : 1. Plasm epinephrine in glycogen-loaded condition markedly increased with workloads, and the difference of epinephrine concentration between glycogen-loaded condition and glycogen -depleted condition was significant(p<0.05) from 120 watt workload upward. 2. Lactate concentration remarkably increased in glycogen-loaded condition with wokloads, and differences among three conditions were significant(p<0.01) at lower workloads. 3. Changes of epinephrine and lactate were similar in all conditions. But changing points of these variables were occurred at lower workloads and higher workloads in glycogen-loaded condition and glycogen-depleted condition, respectively.

악교정 환자의 악교정 수술전후 발음양상에 대한 비교연구

권경환,김수남,이동근,조용민,이숙향 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

The purpose of this study was undertaken to determine the effects of orthognathic surgery on speech. The hyposis stated herein is that functional behaviors of the dentofacial complex, such as speech production, may be adversely affected by deviations of a structural nature(especially, Class III malocclusion) . Twenty adults with Class III malocclusion(13 female and 7 male) were studied preoperative, immediate postoperative and either 6 or 12 months postoperative lateral cephalograms. They had mandibular prognathism and had undergone mandible setback operation. The position of tongue, soft palate(Uvula), hyoid bone, respiratory track width, and pharyngeal depth were assessed on lateral cephalograms with 23 cephalometric variables, ANOVA, Paired t-tests and Pearson's product-moment correlation coefficient tests were used to evalute the operative changes in all cephalometric parameters. A experienced speech and language pathologists performed narrow phonetic transcriptions of tape-recorded words and sentences produced by each of the ninth patients and the recording tapes were analyzed by phonetic computer program(Computerized Speech Lab(CSL) Model 4300BI(U.S.A.)) These judges also recorded their ratings of each patient's overall consonants, hypernasality, hyponasality, and articulation proficiency. The results obtained are as follows ; 1.There were significant changes in distance of posterior pharyngeal wall to tongue (TI-TW2, TS-TW3) after the surgery at 6 months postoperatively(each p<0.01 p<0.05) 2.The posterior tongue point(TI, TS, PPT) moved posteriorly after surgery and remained to its changed position at 6 months postoperatively(p<0.05) . The displacement of tongue was correlated with the movement of mandibular setback amount(p<0.05). The hyoid bone moved posteriorly superiorly after immediate postoperative period. There was significant changes in hyoid bone movement after immediated post-operative period(p<0.05), but returned to its original position during the follow-up period(p>0.05) 3.The soft palate was displaced posteriorly superiorly after immediated operative period and remained to its changed position at 6 months postoperatively(p<0.05) ANS-PNS-SPT angle increasing, PPU-PPPo distance narrowing was showed after surgery, and remained its appearance 6 months postoperactively (p<0.05) 4.There were significant changes in formant value and squre diagram of vowel sound after the orthognathic surgery and the follow-up period. There were significant changes in /ㅅ/sound and posterior tongue sound. 5.The posterior movement of tongue and the posteriosuperior movement of soft palate was correlated with mandibular setback amount after orthognathic surgery. On the vowel squre diagram, the author found that the place of articulation after operation moved downward, backward, upward. 6.In assessing speech abnormalities, dental occlusion should be considered as a contributing factor. The vast majority of subjects with preoperactive misarticulations eliminated or reduced their errors following orthognathic surgery. There was significant difference in speech impovement between pre- and post-operation.

음향방출을 이용한 저어널 베어링의 조기파손감지(Ⅱ) : Part Ⅱ : Intervention of Foreign Particles in Lubrication 윤활유 이물질 혼입의 영향 및 감시

권오양,윤동진,김경웅,정민화 韓國非破壞檢査學會 1994 한국비파괴검사학회지 Vol.14 No.2

일반적으로 회전기기의 저어널 베어링 부분은 윤활유 공급의 부족이나 윤활층에 이물질이 혼입되면 시스템의 고장이나 가동중단 등의 원인이 되기도 한다. 따라서 베어링 손상에 기인하는 사고와 관련된 안전운전 문제와 유지비용의 절감을 위해 여러가지 파괴 및 비파괴시험법들이 사용되어 왔다. 본 연구에서는 저어널 베어링에서 가장 발생하기 쉬운 윤활층에의 이물질이 혼입에 의해 야기되는 베어링 파손의 조기검출을 위해 음향방출 기술을 적용하였으며, 전보의 연구에 이어 좀더 정량적이고 체계적인 실험을 수행하였다. 실험실용으로 직접 제작한 모의베어링 시스템을 이용하여 여러 형태의 인위적인 이물질 흔입 상태를 만들어 실험하였으며 베어링 손상 및 결함 형태의 해석을 위해 AE rms level, 파형분석, AE 변수 등의 여러 파라메터를 사용하여 분석 고찰하였다. 그 결과 AE rms level의 변화가 이물질 흔입의 영향에 민감함을 보여주었으며 AE변수등 다른 정보들로부터 손상 원일별로 신호형태를 확인할 수 있었다. Journal bearings in the rotating machineries are vulnerable to the contamination or the insufficient supply of lubricating oil, which is likely to be the cause of unexpected shutdown or malfunction of these systems. Various destructive and nondestructive testing methods had been used for the reduction of maintenance cost and the operational safety problems due to the accidents related to bearing damages. In this experimental approach, acoustic emission monitoring in employed to the detection of incipient failure caused by intervention of foreign particles most probable in the journal bearing systems. Experimental schedules for the intervention of foreign particles was composed to be more quantitative and systematic than last study in consideration of minimum oil film thickness and particle size . The experiment was conducted under such designed conditions as inserting alumina particles to the lubrication layer in the simulated journal bearing system. Several parameters such as AE rms level, waveform, AE energy distribution and other AE event parameter are used for analysis and characterization of damage source. The results showed that the history of damage was well correlated with the changes of AE rms level and the types of damage source signal can be verified using other informations such an waveform, distribution of AE parameter etc.

체중감량이 유도선수의 Renin, 면역기능 및 유·무산소 운동능력에 미치는 영향

권영우,전승훈,김진해,민경선,민진아 한국스포츠리서치 2001 한국 스포츠 리서치 Vol.12 No.4

The purpose of this study was to investigate the effect of short terms weight reduction period on body composition and body fluid. Seven Judo athletes (21±1.21 yrs) were matched and randomly placed. Body weight reduction period consists of 7 day. Each groups reduced body weight by 3%(2th day from pre weight reduction), 5%(4th day from pre weight reduction, 7%(7th day) and investigated the recovery period on 3 hour after breakfast, lunch, and 1day, 2day after 7% body reduction. Weight reduction reduced weight during weight reduction period then returned to baseline during recovery(p<.05) and decreased LBM, electrolyte, and immune function and then increased in recovery period to baseline. In conclusion, weight reduction of Judo athletes caused the change biological variable and performance and STWR may detrimental effects body' homeostasis.