Efficacy of Gene Transfer and Expression of Novel Recombinant Baculovirus Vector

권태동,홍성갑,Kweon, Tae-Dong,Hong, Seong-Karp The Korea Institute of Information and Commucation 2014 한국정보통신학회논문지 Vol.18 No.8

폴리히드론 프로모터, 수포성구내염 바이러스G, 폴리A, 사이토메가바이러스 프로모터, 강화녹색형광단백질, 단백질전달부위 유전자 등을 포함한 새로운 베큘로바이러스 시스템이 제조되었다. 본 재조합벡터 시스템은 인간 섬유아세포에 적용하여 시험하였고 재조합된 유전자의 전달과 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구로부터 새롭게 제작된 본 베큘로바이러스 시스템이 유전자의 전이와 유전자 발현 면에서 대조 벡터시스템 보다 고효율을 나타내었다. Novel baculovirus vector systems recombined with coding genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD) were constructed. These recombinant baculovirus vector systems were applied into human foreskin fibroblast cells and compared the effects of gene transfer and gene expression of these recombinant baculovirus vector systems with control vector system. From this study, it showed that these novel recombinant baculovirus vector systems were superior efficacy to control vector system in view of gene transfer and gene expression.

고지방식이 섭취 흰쥐에서 운동부하가 골격근의 지방대사율 및 AMPK 발현에 미치는 영향

권태동,손태현,김기훈,류승필,허만동,여윤기,정난희 한국운동영양학회 2003 Physical Activity and Nutrition (Phys Act Nutr) Vol.11 No.3

급,만성 통증의 관리

권태동 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.-

제초제 Propanil 및 그 분해산물인 DCA(3,4-dichloroaniline)와 Humic Monomer들과의 산화적 짝지움반응

권태동,김장억,Kwon, Tae-Dong,Kim, Jang-Eok 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.4

제초제 propanil(3',4'-dichloropropionanilide)과 그 분해산물인 DCA(3,4-dichloroaniline)가 laccase, horseradish peroxidase(HRP) 및 birnessite에 의하여 중개된 oxidative coupling에 의하여 토양 유기물의 구성성분에 병합될 수 있는지를 알기 위하여 토양 유기물의 monomer들과의 반응성을 조사하였다. Propanil 또는 DCA가 단독으로 존재하는 반응조건에서는 산화환원촉매들에 의하여 이들의 전환이 거의 이루어지지 않았거나 상당히 낮은 수준이었다. 그러나 humic monomer들이 있을 때 laccase와 HRP의 경우 propanil은 syringic acid와 DCA는 catechol과 높은 전환율을 나타내었으며, birnessite의 경우 DCA는 protocatechuic acid와 높은 전환율을 나타내었다. DCA의 전환율은 laccase의 경우 catechol과 pH 8.0에서 24시간 동안 반응시킬 때, HRP의 경우 catechol과 pH 3.0에서 2시간 동안 반응시킬 때 가장 높았고, birnessite의 경우 protocatechuic acid와 pH 5.0에서 2시간 동안 반응시킬 때 가장 높았다. Humic monomer의 농도를 증가시킬수록 DCA의 전환율도 증가하였다. Humic monomer 대신 dissolved organic carbon(DOC)이 있을 때 laccase는 DCA를 거의 전환시키지 못 하였으나, HRP는 DCA의 전환율을 크게 증가시켰고, birnessite는 큰 영향을 미치지 않았다. DCA의 전환율은 laccase 단독으로 있을 때 보다 birnessite와 공존할 경우 약 5배 가량 증가된 반면, HRP와 birnessite가 공존할 경우에는 증가되지 않았다. The herbicide propanil and its metabolite, DCA were incubated with oxidative catalysts in the presence or absence of humic monomers to evaluate the incorporation of them into humic substances. Propanil and DCA underwent little or no transformation by oxidatve catalysts in the absence of humic monomers. In the presence of humic monomers, the most effective co-substrate for transformation of propanil was syringic acid by laccase and HRP, that of DCA was catechol by laccase and HRP, and protocatechuic acid by birnessite. The transformation of DCA was the highest when it was incubated with catechol at pH 8.0 during 24 hrs by laccase, and with catechol at pH 3.0 during 2 hrs by HRP, and with protocatechuic acid at pH 5.0 during 2 hrs by birnessite. The DCA transformation increased with increasing concentration of humic monomers. The transformation of DCA was increased with about 5 times when it was incubated with lactase and birnessite together than lactase alone, but that of it was not effected when it was incubated with HRP and birnessite together. When DCA was incubated with dissolved organic carbon in the presence of oxidative catalysts, the transformation of it was not increased by laccase and birnessite but increased by HRP.

Sedation under JCI standard

권태동 대한마취통증의학회 2011 Korean Journal of Anesthesiology Vol.61 No.3

The practice of anesthesia and sedation continues to expand beyond the operating room and now includes the gastroenterology suite, magnetic resonance imaging suites, and the cardiac catheterization laboratory. Nonanesthesiologists frequently administer sedation, in part because of a lack of available anesthesiologists and economic aspect, which emphasizes the safety of sedation. The Joint Commission International (JCI) set a standard responding to this issue indicating that qualified individuals who have drug and monitoring knowledge as well as airway management skills can only administer sedating agents. In Korea, the Ministry of Health and Welfare developed new sedation standards for hospital evaluation, which is similar to the JCI standards. This review intends to help with the understanding of the JCI sedation standard and compare it to the Korean sedation standard.

흰쥐에서 탄수화물 종류가 운동시 당 및 지질대사에 미치는 영향

권태동,전중기,민경선,김형렬,장응찬 대구대학교 기초과학연구소 1998 基礎科學硏究 Vol.15 No.2

The purpose of this study was to investigate the effects of glucose, sucrose, and glucose polymer(GP) ingestion on the carbohydrate and lipid metabolism during treadmill running in rats. Rats were randomly assigned to normal, control and carbohydrate groups. Carbohydrate group was divided into glucose, sucrose and GP ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either normal saline(control) or carbohydrates solutions of formulated glucose(6%), sucrose(6%) and GP(6%) via polyethlene tube to stomach before treadmill run. Exercise was loaded by a treadmill run at the speed of 1 km/hour for 30, 60 and 120 minutes. The results of experiment were as follows: Plasma glucose levels were decreased in proportion to the exercise time in control, but increased at treadmill run 30 minutes and then gradually decreased with the lapse of the exercise time in carbohydrates compared with normal rats. It decreased in control, glucose, sucrose and GP compared with normal rats in decreasing order at treadmill run 120 minutes(% change of plasma glucose level of control, glucose, sucrose and GP compared with normal rats were 67, 74, 87 and 91 in plasma glucose level at treadmill run 120 min., respectively). Plasma lactate levels were increased by the exercise loading compared with normal in control and carbohydrates ingested rats. It increased in control, sucrose and GP in increasing order compared with normal rats, at treadmill run 120 minutes(% change of plasma lactate level of control, glucose, sucrose and GP compared with normal rats were 167, 185, 149 and 114 in plasma lactate level at treadmill run 120 min., respectively). Plasma FFA levels had tendency to increase by the exercise loading compared with normal in control and carbohydrates ingested rats. It showed increase in the sucrose, glucose, GP and control rats in Increasing order compared with normal rats at treadmill run 120 minutes(% change of plasma FFA level of control, glucose, sucrose and GP compared with normal rats were 151, 153, 166 and 152 in plasma FFA level at treadmill run 120 min., respectively). Plasma acetoacetate levels were higher in control compared with carbohydrates ingested rats at treadmill run 30, 60 and 120 minutes. Plasma beta-hydroxybutyrate levels increased by exercise compared with normal rats in control and carbohydrates ingested rats at treadmill run 30, 60, 120 minutes. Plasma insulin levels were decreased in proportion to the exercise time in control compared with normal rats, but in carbohydrates ingested rats increased at treadmill run 30 minutes and then gradually deceased with the lapse of the exercise time compared with normal. In carbohydrates ingested rats, plasma insulin levies were higher than control. Hematocrit did not show any difference between control and carbohydrates rats at treadmill run 30, 60 and 120 minutes. From these experimental results, it is suggested that the exhaustion of skeletal muscle glycogen, the hypoglycemia, the overproduction of lactate and dehydration is delayed in carbohydrates ingested rats. GP of the carbohydrates is considered as the most effective for these effects. Therefore, intake of carbohydrates before exercise, especially GP seems to increase performance in the prolonged moderate exercise.

알콜성 지방간 마우스에서 운동부하가 간손상에 미치는 영향

권태동 한국운동영양학회 2007 Physical Activity and Nutrition (Phys Act Nutr) Vol.7 No.1

The purpose of this study was to investigate the effect of exercise on hepatotoxicity in mouse with alcoholic fatty liver. ICR-mice were divided into 3 experimental groups; Normal group were fed basal diet and water for all experimental period (10 weeks). Alcohol and exercise group were fed basal diet and 30% ethanol for first 5 weeks. And, the next 5 weeks were received basal diet and exercise. The results are as follows: Plasma total-cholesterol, triglyceride and LDL-cholesterol concentration were higher in Alcohol group than Normal groups after 5 weeks. however, totalcholesterol and LDL cholesterol were decreased after 5 weeks of experimental period in Alcohol pluse exercise groups. Liver cholesterol, triglyceride concentration, liver GOT, GPT and LPO were same pattern. Alcohol and exercise group were more decreased in this plasma lipid composition, liver GOT, GPT and LPO than Alcohol group after 5 weeks of experimental period. However, GSH were lower in Alcohol group than Normal groups after 5 weeks and experimental period for 5weeks. The present results demonstrate were exercise training have hepatoprotective effects of alcohol induced hepatotoxicity in rats

조정 및 카누훈련이 폐기능에 미치는 영향

권태동,전중기,김형렬 대구대학교 기초과학연구소 1998 基礎科學硏究 Vol.14 No.2

In the present study, an effort was directed to elucidate the effect of the physical training on the pulmonary function. Nine male athletics major student who have undergone regular physical training for more than five years were randomly chosen as the athletic subjects, and 9 regular male students who have not been engaged in any form of regular physiycal exercise or training were chosen as the non-athletic subjects, and a comparison was made between the two groups. The following were mainly observed by spirometry for the study respiratory rate, tidal volume, vital capacity, maximun voluntary ventilation (MVV), forced expiratory volume for 1 second (FEY, ), percent FEV, to forced vital capacty (FEV 1%), forced expiratory flow for initial 1 liter (FEF 0.2-1.2L), and forced mid-expiratory flow (FEF 25-75%) The results obtained are summarized as follow; 1) The respiratory rate, and tidal volume showed no significant difference between athletes and non-athletes. The VC and MVV in non-athletes was significantly (p< 0.05, p <0.01)increased to 5.552±0.138L, 158.2±3.11L/min comparing with 4.770±0.2444L, 128.3±9.1L/min in non-athletes. 2) FEV, was 4.781±0.193L in athletes and 4.249±0.136L in non-athletes ; FEV 1% 84.12±1.12% in athletes and 73.45±1.79% in non-athletes, both was significantly (p < 0.05)increased in athletes. 3) FEF0.2-1.2L/min was 296.4±11.7L/min in athletes and 227.7±14.5L in non-athletes ; FEF 25-75% was 3.673±0.119L/sec inathletes and 2.810±0.178L/sec in non-athletes, both showing significant(p<0.05) increase in athletes. 4) Some liscussions were made on these results. The lung volumes showed no significant difference between the two groups, but VC, MVV, FEV1, FEV, %, FEFO.2-1.2L and FEF25-75% in athletes were significantly (p<0.05, p<0.01, p< 0.05, p<0.05, p<0.05, p< 0.05 respectively)higher than in non-athletes. It is therefore concluded that the athletes have more powerful respiratory muscles, or higher compliance of the lung and thorax than the non-athletes.

Joint Commission International 관점에서 본 진정요법의 최신지견

권태동 대한의사협회 2011 대한의사협회지 Vol.54 No.12

Although the practice of sedation has markedly increased and expanded beyond the operating room with the development of new diagnostic and minimally invasive surgical procedures, there is no guideline for hospitals to set up a sedation policy in Korea. The Joint Commission International (JCI), which is a division of Joint Commission of United States, has urged Korean hospitals to develop guidelines for sedation. Because JCI defines the sedation standards as a minimum, there is much for Korean medical association and physicians in charge of sedation to do in order to improve the safety of sedated patients and set up an economical and efficient sedation system. There are three main requirements to meet JCI standards. Aqualified individual responsible for sedation should complete a formal training program supervised by an anesthesiologist or experienced practitioner, and adequate facilities and monitoring systems must be available to safely care for patients throughout sedation. Secondly, a documentation process around sedation, including informed consent, pre-operative assessment, the parameters obser-ved during sedation, and discharge criteria, should be organized according to the sedation policy, which in turn should be determined by an institutional sedation committee. Lastly, JCI requires continuous quality improvement activity, which can improve the efficiency of the sedation process and the safety of sedation care. Multi-departmental joint discussion may be necessary to advance the practice of sedation and avoid conflicts among departments. This review aims to highlight the starting points of adiscussionon local standards for sedation.

고지방식이 섭취 흰쥐에서 운동부하가 골격근의 지방대사율 및 AMPK 발현에 미치는 영향

권태동,손태현,김기훈,류승필,허만동,여윤기,정난희 한국운동영양학회 2003 Physical Activity and Nutrition (Phys Act Nutr) Vol.7 No.1