Novel Trimeric Complex for Efficient Uptake of Plasmid Vector into HepG2 Cells

박종구,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.2

Viral and :ion-viral vectors have been used in the delivery of genetic materials into animal cells and tissues, with each approach having pros and cons. Non-viral vectors have many useful merits such as easy preparation, low immunity and size tolerance of a transgene when compared to those of viral vectors. Delivery specificity may be achieved by complex formation between receptor ligands and a non-viral vector. In the present study, non-viral vector systems are investigated in an effort to find a practical delivery means for gene therapy, Receptor-ligand interaction between transferrin-receptor and transferrin was utilized for efficient gene transfer into cancer cells. A plasmid vector, pcDNA3 (LacZ) was ligated with a small duplexed oligo fragment in which a Biotin-VN^(TM) phosphoramidite was placed in the middle of the oligo. The plasmid vector labeled by biotin was then conjugated with biotin-labeled transferrin via streptavidin. This trimeric conjugates were delivered to a hepatoma cell line, HepG2. The delivery efficiency of the trimeric conjugate was 2-fold higher than that of cationic liposomes used for transfection of a plasmid vector. These results demonstrate that a plasmid vector can be efficiently transferred into cells by forming a trimeric complex of plasmid vector-linker-ligand.

충북대학교 학내 버섯 분포상

이종혁, 박성웅, 이화용 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2

Mushroom refers to a group that forms a visual-sized body among fungi, and they are clas- sified into basidiomycetes and ascomycetes. The Chungbuk National University campus is composed of small forests and gardens, so the habitats are diverse. In this study, we investigated the occurrence of mushrooms in the Chungbuk National University campus . The mushroom occurrence monitoring was carried out at 8 sites in the campus from May to October 2021. Total 42 families 80 genera 150 species were investigated in the campus during monitoring period. They are also divided into 90 saprophytic mushrooms, 59 ectomycorrhizal mushrooms and one of unknown species of nutrition acquisition In the future, it is expected to contribute to find forest mushroom resources and understand the ecosystem change in the campus.

骨軟化症에 대한 東西醫學的 文獻考察

박종혁,황영근,정지천 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

고령화와 육체적 활동의 감소로 증가 추세에 있는 骨軟化症의 臨床治療에 도움을 얻고자 歷代醫書와 中醫書, 中醫雜誌를 중심으로 症狀, 病因, 病理, 治法, 治方 등을 東西醫學的으로고찰하였다. 骨軟化症은 골의 석회화 장애로 골밀도가 감소되는 대사성 골질환으로, 東洋醫學에서는 骨??, 骨枯 등의 骨疾患에서 유사한 症狀이 나타나며, 病因은 주로 腎虛로서 서양의학의 신장 질환으로 인한 燐의 재흡수 불량, Vit-D 대사 이상과 유사하다. 症狀으로는 腰痛, 骨痛症, 다발성 골절, 동요성 보행 등이 나타난다. 治法은 補腎을 爲主로 하여 健脾益腎, 滋養氣血, 强壯筋骨 등이 있고, 治方은 六味地黃湯을 위주로 하여, 虎潛丸, 濟生腎氣丸, 大補陰丸 등이 활용되고 있으며, 藥物은 熟地黃, 虎脛骨, 胡挑肉, 紫河車, 社沖, 鹿角膠, 鹿茸 등의 補腎之劑가 주로 사용되고 있다. Osteomalacia is syndrome of divers etiology, characterized pathophysiologically by a failure of normal mineralization of bone and epiphyseal cartilage, This study was performed to investigate causes of disease, pathogenic mechanisms, symptoms, therapies and precriptions through the successive medical literatures, recent chinese medical literatures and chinese medical journals. It is similar to atrophic debility of bones, bone leaning, bone exhaustion, rheum atism involving the bone, osteodynia and cold and heat of bone etc. of oriental medicine. The most principal cause of this is deficiency of kidney, similar to hypo phosphatemia caused by increased renal clearance and deficiency of vitamin D. and the rest are senility, deficiency of spleen, deficiency of qi and deficiency of blood. There are nourishing the kidney and spleen, nourishing the qi and blood, warming and passing the muscle and mac, passing an articulation an invigorating the muscle and bone, in principal therapy. And in medical herbs are rehmanniae radix preparat, corni fructus, discoreae rhizoma, cuscutae semen, tigridis os, juglandis semen. hominis placenta, drynariae rhizoma, eucommiae cortex, cynomorii herba. corvi cornus colla, cervi pantotrichum cornu, moutan cortex, polygoni multiflori radix, angelicae gigantis radix, achyranthis bidentatae radix, cibotii rhizoma, hirudo, eupolyphaga, spatholobi caulis, salviae miltiorrhizae radix, draconis resina. curcumae longae rhizoma. In care there are a sun-bath, exercise, high protein diet and taking vitamin D. And they reduce smoking, coffee, drinking etc.

난소의 소세포암종, 고칼슘혈증형 대세포 변종 : 1예 보고 A Case Report

이종실,이종학,김유경,박선후,고경혁 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.4

운동이 비만 여성의 심근산소소비량과 심전도 QTc 간격에 미치는 영향

이혁종,박윤정,김용권,한구석,진영수 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of exercise staining on MVO_2 and electrocardiogram QTc interval in obese woman and to propose the desirable method for weight loss. Subjects were fifteen obese women for this study. 8 subjects were prolonged QTc interval group and 7 subjects were normal QTc interval. They participated in exercise program consisted of aerobic exercise and weight training exercise at A Medical Center. The body composition including Boyd Weight, BMI, WHR and % Body Fat was measured. The resting and exercise(Bruce protocol 7th min) rate-pressure product(RPP) could be measured with HR and systolic BP for MVO_2 measurement. The electrocardiogram QTc interval was calculated with Bazzett's formula using automated software program. The measured data of this study were as follows: 1) After participating the exercise program consisted of aerobic exercise and weight training exercise, Body Weight, BMI, WHR and % Body Fat were significantly changed in two groups. (p<.05). 2. After participating in the exercise program consisted of aerobic exercise and weight training exercise, the resting and exercise MVO_2 using RPP were significantly changed in two groups(p<.05). 3) After participating in the exercise program consisted of aerobic exercise and weight training exercise, the electrocardiogram QTc interval were decreased but were not significantly changed in prolonged QTc interval group. (p<.29). However, it was significantly decreased in prolonged QTc interval(p<.00).

中風의 治法 중 潛陽法에 대한 문헌고찰

閔健祐,朴鍾赫,鄭智天 동국대학교 한의학연구소 2000 東國韓醫學硏究所論文集 Vol.9 No.-

潛陽法은 平肝治法의 일종으로 刊陽이 上亢해서 나타나는 頭目昏暈, 수족추휵, 구안괘사, 舌乾語澁, 頭痛不止, 猝然倒地 등의 症狀에 쓰는 治法이며 中風의 標實證을 다스리는 목적으로 多用되고 있다. 中風에서의 活用은 淸代에 이르러 본격화되었는데, 葉天士는 "자액식풍, 濡養營絡, 補陰潛陽"을 사용하였고, 張伯龍은 "潛鎭攝納" 하는 치료법을 사용하였다. 中風의 病理가 本虛標實하므로 항상 滋陰, 補水하는 治法과 相互補完的으로 사용되고 있다. 潛陽法이 多用된 병증은 刊胃陰虛, 風陽上擾로 인한 中經絡證과 中腑證 중에서 陽閉證이며, 脫證의 일부와 中風日久로 因한 癲癎에도 사용 되었다. 潛陽藥 중 珍珠母, 石決明, 玳瑁, 牡蠣, 見齒, 龜板, 鱉甲 등 介類의 효력이 뛰어나고, 治方으로는 鎭肝??風陽, 虎潛丸, 固本丹, 復脈湯, 天麻鉤藤飮, 羚羊角湯 등이 활용되고 있다. The methods of checking the exuberance of yang (潛陽法) are a kind of medical treatments Lvhich cure various symtoms caused by hyperactivity of liver-yang, at the same time used for treating apoplexy. Thus, I investigated the application of checking the exuberance of yang to treat stroke. The conclusions are as follows. We can find application of checking the exuberance of yang to apoplexy on Synopsis of the Gold Chamber. After Ye Gui who persisted the theory of endogenous wind, many physicians applied the method of checking the exuberance of yang and this method has usually applied with nourishing yin. The methods of checking the exuberance of yang are concretely applied as follows. 1) a portion of apoplexy involving the channels and collaterals, which is caused by deficiency of \-in and wind-fire pathogen. 2) excessive-syndrome of coma accompanied by heat syndrome. 3) epilepsy caused old apoplexy. The majority of shells for example abalone shell, hawksbill shell, oyster shell, tortoise-shell etc. are the most frequent use of herbal drug which check the exuberance of yang. The precriptions of checking the exuberance of yang are Ho-jam-hwan(虎潛丸), Ko-bon-dan(固本丹), Cheon-ma-gu-deung-eum(天麻鉤藤飮), Chin-gan-sik-pung-tang(鎭肝湜風湯) etc.

Self-check Home Exercises Improves Static and Dynamic Balance, Fall Efficacy, and Quality of Life in Patients with Parkinson`s Disease

Jin-hong Kim,Jun-hyuck Park,Jong-eun Yim 보안공학연구지원센터 2016 International Journal of Bio-Science and Bio-Techn Vol.8 No.6

Parkinson's disease is a chronic progressive disease of the nervous system associated with the basal ganglia and is characterized by rigidity, bradykinesia, tremor, and insta-bility of posture. In addition, it causes indirect disabilities and complications. The aim of this study was to evaluate the effects of self-check home exercise on fall prevention in patient with Parkinson’s disease. The present study included 20 patients who met the study criterion, which was a diagnosis of Parkinson's disease. The present study was conducted over 12 weeks in the exercise therapy center of the G University Hospital. Periodically, once a month at the first, fifth, and ninth-week sessions, a neurosurgeon, a rehabilitation physician, a nurse in charge of neurosurgery programs dedicated to Park-inson's disease, a physiotherapist, and an occupational therapist attended the home exer-cises program to provide group lessons to subjects, to provide feedback regarding the home exercises program, and to carry out a self-monitoring checklist performance rate. The results showed significant differences in TUG, FRTA, FRTP, FES, STS, 10MWT, MBI, and QoL (p<.05). The present study involved showing patients with Parkinson's disease how to easily perform home exercises and how to provide ongoing feedback for motivation. The home exercises program of the present study is expected to improve the lives of patients with Parkinson's disease.

두경부 편평상피세포암 세포주의 VEGF 아형의 분포와 종양증식 및 이종이식성 : relation to xenotransplantability and tumor progression in mice.

김동욱,이종대,박진규,이재형,이병돈,장혁순 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Overexpression of vascular endothelial growth factor(VEGF) is related to tumor progression and xenotransplantability in various human solid tumors, but the specific impact of the VEGF-subtypes is still under discussion. The aim of this study was to analyse a possible association of the major VEGF-isoforms and the growth characteristics of xenotransplanted human head and neck squamous cell carcinoma tumors in nude mice. Seven squamous cell carcinoma cell lines were analysed by quantitative RT-PCR using the Taqman^(TM)-System. We investigated the expression of VEGF-total-mRNA and of the major subtypes VEGF-121, -165, and -189 by using subtype specific primers. The cell lines were xenotransplanted in three mice each, and the data of tumor growth and progression were correlated to the expression of VEGF-isoforms. We also investigated an "growth response rate" measured by tumor growth per detected VEGF-level. Six out of the seven cell lines analysed expressed all isoforms of VEGF in different quantities. One cell line expressed generally low levels of VEGF and no VEGF-189 at all, In this cell line xenotransplantation failed in one mice out of three. In a second cell line transplantation failed in one out of seven mice, too. Success rates for the other five cell lines were 100%. The cell lines with higher transplantation success were expressing higher VEGF-121/165-189 ratios comparing to those without success. In contrast, linearity of tumor growth and lack of necrosis were associated with a lower VEGF-121/165-189 ratio. The findings demonstrate a predominant expression of VEGF-165 and VEGF-189, compared VEGF-121. In highly proliferating tumors this rate appeared to be about 10 times higher than in low proliferating tumors. We conclude that the ratio between the VEGF-subtypes during tumor implantation and growth is a prerequisite for progression and hypothesize an individual and different response of each tumor cell line to VEGF.

음향방출기술에 의한 밸브누설평가 연구(1)

이상국,이선기,이준신,박종혁 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

The objective of this study is to estimate the feasibility of acoustic emission method for the internal leak from the valves. In this study, valve leak tests using various types of specimen simulated seat damage were performed in order to analyzer acoustic emission properties when leaks arise in valve seat. As a result of leak test for specimens simulated valve seat, we conformed that leak sound level increased in proportion to the increase of hole diameter and leak velocity, and decreased in proportion to the increase of leak depth. And also, leak sound level has hysteresis for leak velocity. From the experimental results, it was suggested that the acoustic emission method for monitoring of leak was feasible.

정신분열병 환자의 인지과제수행에서 삼차원적 시공간-운동 방해자극 처리의 특성

정혜정,김재진,박성혁,전종희,김지혜,염태호,이장한,김선일,구정훈,조원근,안석균,이홍식 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.2

Objectives : Abnormalities of cognitive responses to verbal and visual distracter in schizophrenia have been demonstrated in numerous studies but little is known about three dimensional visuospatial-motor distracter processing. The purpose of this study was to investigate the vulnerability of patients with schizophrenia to three dimensional visuospatial-motor distracter. Methods : 14 patients with schizophrenia and 14 normal volunteers were assessed on three dimensional maze tasks requiring on working memory and reasoning ability in the virtual reality environment. Performances were compared according to with- or without-distracter conditions. Results : Patients with schizophrenia showed significantly lower performances than normal control group on cognitive flexibility, simple motor function or motor control and motor coordination, working memory span and delayed performance times. The performance index tended to be more deteriorated, but not significantly, in with-distracter condition than in Without-distracter condition in schizophrenia. Conclusions : Patients with schizophrenia tend to be vulnerable to visuospatial-motor distracter which simulate the real world of three dimensional environment.