홍국 풀을 이용한 김치 제조 및 품질 특성

김현정,박정현,황보미향,이효주,이인선 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.6

Kimchi was prepared with the addition of 2.5% and 5.0% Monascus purpureus koji(MPK) paste (20%) and were fermented at 20C for 18 days. The quality and sensory characteristics of the kimchi were evaluated by analyzing the pH, acidity, number of viable cells, the concentration of reducing sugar, and sensory properties during fermentation. The pH and titratable acidity of the kimchi prepared with MPK(MPK kimchi) were higher and lower, respectively, than those of the control kimchi. The MPK kimchi showed high 'L' and 'b' values during storage, but the 'a' values were low. The contents of the reducing sugar of the MPK kimchi tended to increase during fermentation, particularly after six days. The number of total microbial cells, lactic acid bacteria and yeast in the MPK kimchi were lower than those of the control kimchi until 3 days of fermentation. However, the number of these bacteria in the MPK kimchi and the control kimchi after six days of fermentation was similar. The sensory score of the kimchi with 2.5% and 5.0% added MPK paste were significantly higher than the control groups in terms of the sweetness and overall acceptability.

납노출지표와 적혈구내 protein kinase C 활성도의 연관성

황규윤,황보영,안현철,김용배,리갑수,이성수,안규동,이병국 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

목적 : Protein kinase C는 칼슘-인지질 의존형인산화 효소로 'H VJ'tfO에서 납에 의하여 활성화되지만, 납에 노출된 인체내에서 그 영향을 평가한 경우는 얼다. 본 연구의 목적은 납에 직업적으로 노출되는 근로자를 대상으로 납 노출이 적혈구막의 단백질내에서 PKC 활성에 의한 인산화 수준에 영향을 주는지 알아보고자 하였다. 방법 : 1998년부터 국내 납 근로자를 대상으로 납에 의한 건강 영향조사가 4년간의 코호트 연구로 실행하였다. 1차년도에 630명의 납 근로자와 135명의대조군이 조사되었고 이들중 본 연구에서는 직업적노출이 되는 사업장에 근무하는 212명의 근로자를대상하였다. 156명의 남자와 56명의 여자 근로자를 대상으로 인구학적, 과거병력, 직업력 등을 구조화된 설문과 면접으로 조사되었고 납 노출 평가는 혈중 납농도 및 ZPP, 경골중 납농도를 측정하였다. PKC의 활성도는 적혈구막 단백질내 PKC 의존형인산화 수준으로 평가하였다. 적혈구막 단백질인spectrin과 band 4.9의 후인산화수준을 측정하여각 납 노출지표(혈중 납, ZPP, 경골중 납. 노출기간)와의 관련성은 다중회귀분석을 이용하였다. 결과 : 조사대상자의 평균(SD) 연령은39.1(10.0)세, 근무기간은 8.1(6.5)년 이었으며, 경골중 납농도는 범위가 0.8에서 290.8 trg Pb/gbone mineral로 평근(SD) 34.4(35.2) rig Pb/gbone mineral이었다. 적혈구막 단백질의 후인산화수준은 개인간 변이가 매우 컸으며, spectrin은 평균(SD) 540 7(304.1), band 4.9 SfkDa는198.6(78.2), 48 kDa는 247.7(83.3) PSL이었다.경골중 납농도와 노출 기간은 이들 후인산화 수준과역상관성을 보였으나(p(0.05), 혈중 납 농도와 ZPP는 상관성이 없었다(p)0.05). 가능성 있는 혼란변수를 통제한 상태에서도 경골중 납 농도와 노출기간은 이들 후인산화수준과 유의한 회귀계수를 나타내었다. 결론 : 만성적 납 노출에 의하여 적혈구내 PKC활성도는 영향을 받아 증가되어있는 것으로 평가되어 적혈구막 단백질의 인산화수준은 납의 노출지표로 이용될 수 있을 것이다. 납의 신경독성은 부분적으로 PKC의 활성도왁 관련되어 있을 기전을 배제하기 어렵기 때문에 PfC 활성도와 신경행동학적 기능과의 관련성 평가가 진행되어야 할 것이다. Objectives : Protein kinase C(PKC) , a calcium and phospholipid dependent enzyme, is activated by lead in vitro at picomolar concentrations. However, the effect of lead on PKC has never been studied in a human population. The purpose of the study was to evaluate whether lead exposure was associated with PKC mediated-phosphorylation in erythrocytes among lead workers. Methods : Two hundred and twelve lead workers were studied. To determine the levels of phosphorylation ir vivo, an in vivo back phosphorylation technique was used by adding PKC and γ-32P to preparations of erythrocyte membranes. We measured back phosphorylations of erythrocyte membrane proteins, spectrin, and 52 kDa and 48 kDa, as an indirect measure of PKC activation if vivo. Results : The mean(SD) age and exposure duration was 39.1(10.0) years and 8.1(6.5) years, respectively. Tibial lead ranged from 0.8 to 290.8 μg Pb/g bone mineral with a mean (SD) of 34.4(35.2) μg Pb/g bone mineral. The means(SD) of back phosphorylation levels of the three proteins were 540.7(304.1), 198.6(78.2), and 247.7(83.3) photostimulated luminescence units (PSL), respectively, by phosphoimager. After adjustment for potential confounding factors, tibial lead and exposure duration were significantly and inversely associated with back phosphorylation levels. One unit of increase in tibial lead (1 μg Pb/g bone mineral) is associated with a decline in spectrin, band 4.9 52 kDa, and band 4.9 48 kDa back phosphorylation levels by 1.4(p〈0.05), 0.34(p〈0.05), and 0.47(p〈0.01), respectively However, there were no associations between the back phosphorylation levels and either blood lead or ZPP levels. Conclusions : These findings suggest that the PKC activity in erythrocytes is increased by chronic lead exposure and that erythrocyte membrane protein phosphorylation may be a biomarker of lead exposure.

Effects of Morus alba L. and Angelica keiskei on Dexamethasone-induced Skeletal Muscle Atrophy through Muscle Protein Metabolism

Hyun Hwangbo,Seon Yeong Ji,Min Yeong Kim,Jae Hyun Yoon,Seong Un Jeong,Tae Hee Kim,Yung Hyun Choi 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

A Mixture of Morus alba and Angelica keiskei Leaf Extracts Improves Muscle Atrophy by Activating the PI3K/Akt/mTOR Signaling Pathway and Inhibiting FoxO3a In Vitro and In Vivo

Hwangbo Hyun,Kim Min Yeong,Ji Seon Yeong,Kim Da Hye,Park Beom Su,Jeong Seong Un,Yoon Jae Hyun,Kim Tae Hee,Kim Gi-Young,Choi Yung Hyun 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.12

Muscle atrophy, which is defined as a decrease in muscle mass and strength, is caused by an imbalance between the anabolism and catabolism of muscle proteins. Thus, modulating the homeostasis between muscle protein synthesis and degradation represents an efficient treatment approach for this condition. In the present study, the protective effects against muscle atrophy of ethanol extracts of Morus alba L. (MA) and Angelica keiskei Koidz. (AK) leaves and their mixtures (MIX) were evaluated in vitro and in vivo. Our results showed that MIX increased 5-aminoimidazole4-carboxamide ribonucleotide-induced C2C12 myotube thinning, and enhanced soleus and gastrocnemius muscle thickness compared to each extract alone in dexamethasone-induced muscle atrophy Sprague Dawley rats. In addition, although MA and AK substantially improved grip strength and histological changes for dexamethasone-induced muscle atrophy in vivo, the efficacy was superior in the MIX-treated group. Moreover, MIX further increased the expression levels of myogenic factors (MyoD and myogenin) and decreased the expression levels of E3 ubiquitin ligases (atrogin-1 and muscle-specific RING finger protein-1) in vitro and in vivo compared to the MA- and AK-alone treatment groups. Furthermore, MIX increased the levels of phosphorylated phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and mammalian target of rapamycin (mTOR) that were reduced by dexamethasone, and downregulated the expression of forkhead box O3 (FoxO3a) induced by dexamethasone. These results suggest that MIX has a protective effect against muscle atrophy by enhancing muscle protein anabolism through the activation of the PI3K/Akt/mTOR signaling pathway and attenuating catabolism through the inhibition of FoxO3a.

P287 : Q-Switched Nd:YAG laser treatment in melasma patients

( Hyun Hwangbo ),( Seung Hyun Moon ),( Taek Geun Lee ),( Tae Gwang Kwon ),( Hyun Ho Son ),( Sook Kyung Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Melasma treatment remains challenging despite various LASER available, because of potential side effects and high recurrence. Q-switched Nd-YAG LASER using a collimated hand piece, the conventional method, has been used widely to treat melasma. However, method with a fractional hand piece became promising therapeutic method recently. Objectives: We sought to compare Q-switched Nd:YAG LASER with a fractional hand piece to Q-switched Nd:YAG LASER with a collimated hand piece for melasma treatment. Methods: 12 patients were treated with Q-switched Nd:YAG LASER with standardized parameter in 6 sessions of every other week. At each session, half was treated with fractional hand piece and the other was teated with collimated hand piece. All process was conducted under double blinded. The results were evaluated based on 2 dermatologists`` decision and patients`` satisfactory score. Results: On the questionnaire, 7 patients showed better improvement on fractionated site of the face and 5 patients showed better response on the opposite site. Based on clinical photograph, One dermatologist concluded same as the answer of patients. But the another concluded half of the patients showed better improvement on fractional side, and the other showed better result on the opposite side. Conclusion: Melasma treatment using Q-switched Nd:YAG LASER with a fractional hand piece didn``t have superiorityon this study. Further studies about optimal parameter with longer period are required.

Auranofin Enhances Sulforaphane-Mediated Apoptosis in Hepatocellular Carcinoma Hep3B Cells through Inactivation of the PI3K/Akt Signaling Pathway

( Hyun Hwangbo ),( So Young Kim ),( Hyesook Lee ),( Shin-hyung Park ),( Su Hyun Hong ),( Cheol Park ),( Gi-young Kim ),( Sun-hee Leem ),( Jin Won Hyun ),( Jaehun Cheong ),( Yung Hyun Choi ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.5

The thioredoxin (Trx) system plays critical roles in regulating intracellular redox levels and defending organisms against oxidative stress. Recent studies indicated that Trx reductase (TrxR) was overexpressed in various types of human cancer cells indicating that the Trx-TrxR system may be a potential target for anti-cancer drug development. This study investigated the synergistic effect of auranofin, a TrxR-specific inhibitor, on sulforaphane-mediated apoptotic cell death using Hep3B cells. The results showed that sulforaphane significantly enhanced auranofin-induced apoptosis by inhibiting TrxR activity and cell proliferation compared to either single treatment. The synergistic effect of sulforaphane and auranofin on apoptosis was evidenced by an increased annexin-V-positive cells and Sub-G1 cells. The induction of apoptosis by the combined treatment caused the loss of mitochondrial membrane potential (ΔΨm) and upregulation of Bax. In addition, the proteolytic activities of caspases (-3, -8, and -9) and the degradation of poly (ADP-ribose) polymerase, a substrate protein of activated caspase-3, were also higher in the combined treatment. Moreover, combined treatment induced excessive generation of reactive oxygen species (ROS). However, treatment with N-acetyl-L-cysteine, a ROS scavenger, reduced combined treatment-induced ROS production and apoptosis. Thereby, these results deduce that ROS played a pivotal role in apoptosis induced by auranofin and sulforaphane. Furthermore, apoptosis induced by auranofin and sulforaphane was significantly increased through inhibition of the phosphoinositide 3-kinase (PI3K)/Akt pathway. Taken together, the present study demonstrated that down-regulation of TrxR activity contributed to the synergistic effect of auranofin and sulforaphane on apoptosis through ROS production and inhibition of PI3K/Akt signaling pathway.

Corni Fructus attenuates testosterone-induced benign prostatic hyperplasia by suppressing 5α-reductase and androgen receptor expression in rats

Hyun Hwangbo,Da He Kwon,Eun Ok Choi,Min Yeong Kim,Kyu Im Ahn,Seon Yeong Ji,Jong Sik Kim,Kyung-Il Kim,No-Jin Park,Bum Hoi Kim,Gi-Young Kim,Su-Hyun Hong,Cheol Park,Ji-Suk Jeong,Yung Hyun Choi 대한지역사회영양학회 2018 Nutrition Research and Practice Vol.12 No.5

BACKGROUND/OBJECTIVES: Benign prostatic hypertrophy (BPH) is a major cause of abnormal overgrowth of the prostate mainly in the elderly. Corni Fructus has been reported to be effective in the prevention and treatment of various diseases because of its strong antioxidant effect, but its efficacy against BPH is not yet known. This study was designed to evaluate the therapeutic efficacy of Corni Fructus water extract (CF) in testosterone-induced BPH rats. MATERIALS/METHODS: To induce BPH, rats were intraperitoneal injected with testosterone propionate (TP). Rats in the treatment group were orally administered with CF with TP injection, and finasteride, which is a selective inhibitor of 5α-reductase type 2, was used as a positive control. RESULTS: Our results showed that the increased prostate weight and histopathological changes in BPH model rats were suppressed by CF treatment. CF, similar to the finasteride-treated group, decreased the levels of testosterone and dihydrotestosterone by TP treatment in the serum, and it also reduced 5α-reductase expression and concentration in prostate tissue and serum, respectively. In addition, CF significantly blocked the expression of the androgen receptor (AR), AR co-activators, and proliferating cell nuclear antigen in BPH rats, and this blocking was associated with a decrease in prostate-specific antigen levels in serum and prostate tissue. CONCLUSIONS: These results suggest that CF may weaken the BPH status through the inactivation of at least 5α-reductase and AR activity and may be useful for the clinical treatment of BPH.

Inhibitory Effects of Corni Fructus on Testosterone-induced Benign Prostatic Hyperplasia in Rats

Hyun Hwangbo,Eun Ok Choi,Kyung-Il Kim,Son-Ho Yoon,Jung-Sub Jung,No-Jin Park,Ji-Suk Jeong,Jin-Woo Jeong,Cheol Park,Su Hyun Hong,Jae-Hun Cheong,Yung Hyun Choi 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Mixture of Corni Fructus and Schisandrae Fructus ameliorates testosterone-induced benign prostatic hyperplasia through regulating 5α-reductase 2 and androgen receptor

Hyun Hwangbo,Yung Hyun Choi 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

Regulating of Steroid 5 Alpha-Reductase 2 and Androgen Receptor Signaling Pathway by Schisandra Fructus Extract in Testosterone-Induced Prostatic Hyperplasia

Hyun Hwangbo,Yung Hyun Choi 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Benign prostatic hyperplasia (BPH) is a benign enlargement of the prostate that interferes with normal urine flow. Particularly, the increase in dihydrotestosterone (DHT) level by the activation of steroid 5α-reductase 2 (SRD5A2), and the promotion in the binding of DHT to androgen receptor (AR) play an important role in the progress of BPH. We investigated whether Schisandra Fructus extract (SF) could improve testosterone-induced BPH. To induce BPH, Sprague Dawley rat was subcutaneously injected with testosterone propionate daily for 8 weeks followed by SF and finasteride. Our results showed that the prostate index and histological changes characteristic of BPH, were increased in the BPH model. SF treatment decreased prostate weight and epithelial cell thickness. Also, the levels of BPH-induced serum testosterone, DHT and PSA were reduced by SF. Furthermore, we confirmed that the SF suppressed the expression of SRD5A2, prostate-specific antigen, and AR, which are prostatic hyperplasia biomarkers. These results suggest that SF could be presented as a therapeutic agent for BPH.