동아의료원에서 실시한 양수천자 90례의 세포유전학적 분석

한진영,이경미,신상동,차문석,제구화 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.2

자간전증 임부의 혈청내 산화질소와 혈소판 활성화인자의 변화

제구화(Goo Hwa Je),이병재(Byoung Jae Lee),황태웅(Tae Woong Hwang),백동훈(Dong Hoon Baek),차문석(Moon Seok Cha) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7

목적 : 자간전증 환자들의 말초 혈액 내에서 산화질소 (Nitric oxide, NO)와 혈소판 활성화인자 (Platelet activating factor, PAF)의 농도를 측정하여 정상 임신과의 차이를 알아보고자 하였다. 방법 : NO의 농도는 Stuehr의 방법을 사용하여 측정하였고, PAF의 농도는 [3H]PAF scintillation proximity assay (SPA) system (Amersham Pharmacia Biotech)로 측정되었다. 결과 : NO의 농도는 정상 임신군 2.036±0.744 umol/L, 경증 자간전증 5.891±0.481 umol/L, 중증 자간전증 5.535±0.343 umol/L로 측정되어 정상 임신군에 비해 자간전증에서 높은 결과를 보였고 (p<0.05), 경증과 중증의 자간전증 임부간에는 통계학적으로 유의한 차이가 없었다 (p>0.05). PAF의 농도는 정상 임신군 435.8±59.5 ng/㎖, 경증 자간전증군 789.1±63.9 ng/㎖, 중증 자간전증 928.9±32.3 ng/㎖로 측정되어 정상 임신군에 비해 자간전증에서 높게 측정되었고 (p<0.05), 경증과 중증의 자간전증 임부간에는 통계학적으로 유의한 차이가 없었다 (p>0.05). NO와 PAF농도간에의 상관계수 (R)는 0.44012 (p<0.05)로 유의한 양적 상관관계를 보였다. 출생시 태아 체중은 정상 임신군 3242.5±374.1 gm, 경증 자간전증 2195.6±630.3 gm, 중증 자간전증 1655.9±557.9 gm으로 각 군 상호간에 통계학적으로 유의한 차이를 보였다 (p<0.05). NO와 출생시 태아 체중간에의 상관계수 (R)는 -0.59237 (p<0.05)로 유의한 음적 상관관계가 있었고, PAF와 출생시 태아 체중간에의 상관계수 (R)는 -0.33906 (p<0.05)로 유의한 음적 상관관계가 있었다. 결론 : NO와 PAF는 자간전증의 병태생리에 관여할 것으로 사료되며, PAF는 자간전증의 조기발견에 유용한 예측 인자로서 사용할 수 있을 것으로 사료된다. Objective : The aim of this study is to determine whether any association exists between preeclampsia and the maternal serum level of nitric oxide (NO) and platelet activating factor (PAF). Methods : NO concentrations were measured using Stuehr`s method and PAF concentrations were measured with [3H]PAF scintillation proximity assay (SPA) system (Amersham Pharmacia Biotech). Data were analyzed with SAS windows version 6.12, with significance established at p<0.05. Results : The mean (±SD) maternal serum concentrations of NO were significantly higher in the group with severe preeclampsia (5.535±0.343 ug/㎖) and mild preeclampsia (5.891±0.481 ug/㎖) than in the normotensive pregnancy group (2.036±0.744 ug/㎖) (p<0.05). The mean (±SD) maternal serum concentrations of PAF were significantly higher in the group with severe preeclampsia (928.9±32.3 ng/㎖) and mild preeclampsia (789.1±63.9 ng/㎖) than in the normotensive pregnancy group (435.8±59.5 ng/㎖) (p<0.05). The mean birth weight was lower in the mild and severe preeclampsia groups than in the normotensive pregnancy group (p<0.05). Conclusion : Maternal serum NO and PAF concentrations were more increased in preeclampsia than normotensive pregnancy group. NO may therefore serve as compensatory mechanism for vasoconstriction of preeclampsia and PAF as a marker for the risk of preeclampsia.

Development and Validation of Primary Method for the Determination of Glucose in Human Serum by Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry and Comparison with Field Methods

Lee, Hwa Shim,Lee, Jong Man,Park, Sang Ryoul,Lee, Je Hoon,Kim, Yong Goo Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.6

Glucose is a common medical analyte measuring in human serum or blood samples. The development of a primary method is necessary for the establishment of traceability in measurements. We have developed an isotope dilution liquid chromatography tandem mass spectrometry as a primary method for the measurement of glucose in human serum. Glucose and glucose-$^{13}C_6$ in sample were ionized in ESI negative mode and monitored at mass transfers of m/z 179/89 and 185/92 in MRM, respectively. Glucose was separated on $NH_2P$-50 2D column, and the mobile phase was 20 mM $NH_4OAc$ in 30% acetonitrile/70% water. Verification of this method was performed by the comparison with NIST SRMs. Our results agreed well with the SRM values. We have developed two levels of glucose serum certified reference material using this method and distributed them to the clinical laboratories in Korea as samples for proficiency testings. The expended uncertainty was about 1.2% on 95% confidence level. In proficiency testings, the results obtained from the clinical laboratories showed about 3.6% and 3.9% RSD to the certified values. Primary method can provide the traceability to the field laboratories through proficiency testings or certified reference materials.

검진시간 데이터 분석에 기반한 효율적인 종합검진순서에 관한 연구

민겸제(Guem Je Min),이구연(Goo Yeon Lee),최창열(Chang Yeol Choi),김화종(Hwa Jong Kim) 대한전기학회 2014 정보 및 제어 심포지엄 논문집 Vol.2014 No.4

Clinical Practice Guideline for Cardiac Rehabilitation in Korea

Chul Kim,성지동,Jong Hwa Lee,Won-Seok Kim,Goo Joo Lee,Sungju Jee,Il-Young Jung,Ueon Woo Rah,Byung Ok Kim,Kyoung Hyo Choi,Bum Sun Kwon,유승돈,Heui Je Bang,Hyung-ik Shin,Yong Wook Kim,Heeyoune Jung,Eung Ju Ki 대한흉부외과학회 2019 Journal of Chest Surgery (J Chest Surg) Vol.52 No.4

Background: Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and 3 additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea. Methods: This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of 33 authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and 2 general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, 3 rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified. Results: CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers cardiovascular mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR. Conclusion: Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.

아스피린을 처리한 양막세포주 WISH세포에서 mitogen-activated protein kinase를 통한 cyclooxygenase-2 발현의 증가

차문석,제구화,곽종영 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.12

목적 : 최근 양막 세포주인 WISH세포를 아스피린으로 처리한 경우 cyclooxygenase-2(Cox-2)의 발현이 증가된다는 것이 보고되었으나 그에 대한 기전은 아직 밝혀져 있지 않다. WISH 세포에서 아스피린에 의한 Cox-2 발현 증가에 관여하는 신호전달 경로를 알고자 본 연구를 시행하였다. 연구방법 : WISH 세포가 들어 있는 배양액에 단백질 인산화 효소의 억제제들을 첨가한 1시간 뒤에 아스피린(1 mM )을 투여하였다. 처리 6 시간 후 세포를 분리하여 Cox-1과 Cox-2의 발현 정도를 Western blot법을 이용하여 측정하였으며 프로스타글란딘 E2(PGE2) 와 트롬복산B2(TXB2)의 생성은 enzyme immunoassay 방법으로 측정하였다. Western blot 상에서 extracellular signal-regulated kinase(ERK)가 인산화되는 정도로서 mitogen-activated protein kinase(MAPK)활성을 비교하였다. 결과 : 아스피린 단독 처리 1시간 후부터 Cox-2의 발현이 증가하여 6 시간 후까지 증가하였다. Genistein(tyrosine protein kinase 억제제)이나 PD98059(MAPK kinase 억제제)는 아스피린에 의하여 증가된 Cox-2의 발현을 억제하였으나 H-89(protein kinase A 억제제), bis-indolylmaleimide(protein kinase C억제제), 및 LY294002(phosphoinositide 3-kinase 억제제)는 영향이 없었다. Cox-2발현과 일치하여 ERK의 인산화가 아스피린을 처리한 세포에서 증가하였다. 아스피린을 처리한 세포에서 PGE2와 TXB2의 생성은 거의 억제되었으나 PD98059에 의하여 회복되지는 않았다. PD98059 자체는 TXB2 의 생성을 부분적으로 억제하였다. 결론 : 양막세포 유래 WISH세포에서 아스피린에 의한 Cox-2발현의 증가에는 ERK/MAPK가 관여한다는 것을 시사하고 있다. Objective : We recently found that aspirin alone increased the expression of cyclooxygenase-2(Cox-2) in human amnionic WISH cells. The purpose of this study was to evaluate which signaling pathway(s) is involved in aspirin-induced Cox-2 expression in WISH cells. Methods : Cultured WISH cells were pretreated with protein kinase inhibitors for 1 hour and then treated with 1 mM aspirin. The changes of Cox-2 expression and phosphorylation of mitogen-activated protein kinase(MAPK) were measured by using western blot. Using enzyme immunoassay method, the production of prostaglandin E2(PGE2) and thromboxane B2(TXB2) was measured. Results : Direct addition of aspirin to WISH cells caused an increase of Cox-2 protein. By treatment with 10 μM genistein(tyrosine kinase inhibitor) or 10 μM PD98059(MAPK kinase inhibitor), the aspirin-induced increase in the level of Cox-2 was inhibited. However, other kinase inhibitors such as H-89(protein kinase A inhibitor), bis-indolylmaleimide(protein kinase C inhibitor), or LY294002(phosphoinositide 3-kinase inhibitor) did not inhibit the effect of aspirin. Consistent with increased expression of Cox-2, treatment of cells with aspirin activated extracellular signal-regulated kinase(ERK) of MAPK, as revealed by increased phosphorylation. Genistein and PD98059 also prevented the phosphorylation of ERK elicited by aspirin. Production of PGE2 and TXB2 was decreased with aspirin but the inhibition was not reversed by PD98059. PD98059 alone had inhibitory effect on the production of TXB2. Conclusion : These results suggest that ERK/MAPK pathway is involved in the aspirin-induced expression of Cox-2 in WISH cells.

특발성 과호산구성 증후군에 병발한 Immunotactoid Glomerulopathy 1예

이정득,우제영,윤영석,강성구,방병기,양기화,지영희,심상인 대한내과학회 1992 대한내과학회지 Vol.42 No.5

저자들은 수술 전 시행한 말초혈액검사에서 우연히 호산구증다증이 발견되어 여러 검사를 거쳐 과호산구성 증후군으로 진단된 환자가 중증 단백뇨의 소견을 보여 신장 조직검사를 실시하였다. 조직검사 결과 광학현미경 소견상 신사구체 모세관 기저막의 비후와 부종성 변화를 보이는 세포 및 섬유성 물질에 의하여 모세관 내강이 막혀있는 소견과 면역형광현미경 검사상 IgG, C3가 모세관 벽을 따라 침착된 소견을 보였고 전자현미경 검사상 내피세포 하부의 기저막에 직경 20nm의 섬유성 물질의 침착을 보였고 유전분증의 진단적 가치를 가지는 염색법인 Congo red에 음성을 보였다. 이상과 같은 소견은 immunotactoid glomerulopathy에 합당하다고 생각되어 문헌고찰과 함께 보고하는 바이다. The immunotactoid glomerulopathy is a recently described entity characterized histologically by highly organized ultrastructural deposits that appears to be composed of immunoglobulin and complement and are negative for amyloid by Congo red stain. The idiopathic hypereosinophilic syndrome is a disease characterized by idiopathic eosinophilia in blood and eosinophilic infiltration of multiple organs. Although all organ systems can be virtually involved, renal involvement is rare. The authors have experienced a case of idiopathic hypereosinophilic syndrome in a 18-year-old male who showed asymptomatic proteinuria of nephrotic range. Renal biopsy revealed the findings of thickening of the glomerular capillary basement membranes and deposits within the capillary lumen by light microscopy. Immunofluoresence microscopy disclosed IgG and C3 in mesangial regions and capillary walls. Electron microscopy showed highly organized, fibrillary structures measuring about 20 nm in the mesangial matrix and basement membrane. Congo red staining for amyloid was negative. This case was thought to represent an instance of immunotactoid glomerulopathy.

Clinical Practice Guideline for Cardiac Rehabilitation in Korea

Chul Kim,성지동,Jong Hwa Lee,김원석,Goo Joo Lee,Sungju Jee,Il-Young Jung,Ueon Woo Rah,Byung Ok Kim,Kyoung Hyo Choi,Bum Sun Kwon,Seung Don Yoo,Heui Je Bang,Hyung-Ik Shin,김용욱,Heeyoune Jung,Eung Ju Kim,Jung Hw 대한재활의학회 2019 Annals of Rehabilitation Medicine Vol.43 No.3

Objective Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and three additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea. Methods This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of 33 authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and two general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, three rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified. Principal Conclusions CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers cardiovascular mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR. Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.

Clinical Practice Guideline for Cardiac Rehabilitation in Korea: Recommendations for Cardiac Rehabilitation and Secondary Prevention after Acute Coronary Syndrome

Chul Kim,Jidong Sung,Jong Hwa Lee,Won-Seok Kim,Goo Joo Lee,Sungju Jee,Il-Young Jung,Ueon Woo Rah,Byung Ok Kim,Kyoung Hyo Choi,Bum Sun Kwon,Seung Don Yoo,Heui Je Bang,Hyung Ik Shin,Yong Wook Kim,정희연,Eu 대한심장학회 2019 Korean Circulation Journal Vol.49 No.11

Though clinical practice guidelines (CPGs) for cardiac rehabilitation (CR) are an effective and widely used treatment method worldwide, they are as yet not widely accepted in Korea. Given that cardiovascular (CV) disease is the second leading cause of death in Korea, it is urgent that CR programs be developed. In 2008, the Government of Korea implemented CR programs at 11 university hospitals as part of its Regional Cardio-Cerebrovascular Center Project, and 3 additional medical facilities will be added in 2019. In addition, owing to the promotion of CR nationwide and the introduction of CR insurance benefits, 40 medical institutions nationwide have begun CR programs even as a growing number of medical institutions are preparing to offer CR. The purpose of this research was to develop evidence-based CPGs to support CR implementation in Korea. This study is based on an analysis of CPGs elsewhere in the world, an extensive literature search, a systematic analysis of multiple randomized control trials, and a CPG management, development, and assessment committee comprised of thirty-three authors—primarily rehabilitation specialists, cardiologists, and thoracic surgeons in 21 university hospitals and 2 general hospitals. Twelve consultants, primarily rehabilitation, sports medicine, and preventive medicine specialists, CPG experts, nurses, physical therapists, clinical nutritionists, and library and information experts participated in the research and development of these CPGs. After the draft guidelines were developed, 3 rounds of public hearings were held with staff members from relevant academic societies and stakeholders, after which the guidelines were further reviewed and modified. CR involves a more cost-effective use of healthcare resources relative to that of general treatments, and the exercise component of CR lowers CV mortality and readmission rates, regardless of the type of coronary heart disease and type and setting of CR. Individualized CR programs should be considered together with various factors, including differences in heart function and lifestyle, and doing so will boost participation and adherence with the CR program, ultimately meeting the final goals of the program, namely reducing the recurrence of myocardial infarction and mortality rates.

The Role of Vascular Endothelial Growth Factor-Induced Endogenous Nitric Oxide in Proliferation of Trophoblastic BeWo Cells

( Moon Seok Cha ),( Myoung Seok Han ),( Goo Hwa Je ),( Hyun Ho Kim ),( Jong Young Kwak ) 대한산부인과학회 2001 대한산부인과학회 학술대회 Vol.87 No.-