Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

건조 상추 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.3

상추는 가장 선호하는 녹색 채소 중 하나이다. 상추는 폴리페놀성 화합물을 비롯한 다양한 성분을 함유하고 있으며, 항균, 항산화, 항염증 등의 생리활성을 가지고 있는 것으로 알려져 있다. 본 연구에서는 건조상추의 에탄올 추출물(DLE)을 제조하고 이들의 항염증 활성을 연구하였다. DLE의 항염증 활성을 측정하기 위하여 LPS로 활성화된 마우스 대식세포 RAW 264.7 세포주에서 nitric oxide (NO) 생성을 측정하였다. DLE는 세포주의 생존에는 영향을 미치지 않으면서 NO 생산을 현저하게 저해하였다. DLE에 의해 염증 유전자인 iNOS와 COX-2의 유전자와 단백질의 발현이 모두 감소하였으며, 6개의 염증관련 cytokine 유전자(IL-1α, IL-1β, IL-1F6, TNF-α, CSF2, 그리고 CXCL10)의 발현이 모두 감소하였다. 또한, DLE의 처리는 MAPKs 경로의 인산화를 모두 저해하였으며, NF-κB p65의 핵으로의 이동을 저해하였다. 이러한 결과는 DLE의 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 시사한다. 또한, DLE는 농도의존적으로 reactive oxygen species (ROS)의 생산을 저해하였으며, hemeoxygenase-1 (HO-1) 단백질의 발현을 증가시켰으며, HO-1의 전사조절인자인 Nrf2의 핵으로의 이동을 증가시켰다. 결론적으로, 이러한 연구결과는 DLE가 염증관련 유전자의 발현을 감소시키며, MAPKs, NF-κB, 그리고 Nrf2/HO-1 등 다양한 경로를 조절함으로써 항염증 활성을 가지는 것을 제시한다. Lettuce (Lactuca sativa L.) is one of the most popular green leafy vegetables, and it contains various beneficial components including polyphenolic compounds and has been known to possess various biological functions such as anti-microbial, anti-oxidative, and anti-inflammatory activities. In the present study, we prepared ethanol extract of dried lettuce (DLE) and investigated its anti-inflammatory activity. To evaluate the anti-inflammatory activity of DLE, nitric oxide (NO) production was measured in LPS-activated mouse macrophage RAW 264.7 cells. DLE significantly suppressed NO production in these cells without affecting cell viabilities while resveratrol was used as a positive control. DLE dramatically decreased the expression of pro-inflammatory genes such as iNOS and COX-2 at the mRNA and protein levels and reduced the expression of several cytokines including IL-1α, IL-1β, IL-1F6, TNF-α, CSF2 and CXCL10. In addition, DLE suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating DLE shows its anti-inflammatory activity via regulating MAPKs pathway and NF-κB pathways. And also, DLE reduced the production of reactive oxygen species in a dose-dependent manner. DLE increased HO-1 protein expression, and also increased the nuclear translocation of Nrf2. Overall, our results suggest that lettuce down-regulate various pro-inflammatory genes and have its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

연잎, 연자육, 연자방 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

연은 아시아 국가에서 음식과 약재로 널리 사용되는 재료이다. 본 연구에서는 연의 잎(leaf, NL), 자육(seed, NS), 자방(seedpod, NSP)으로부터 에탄올 추출물을 제조하고 이들의 항염증 활성과 작용기전을 규명하였다. 이들의 항염증 활성을 연구하기 위하여 LPS로 자극된 RAW 264.7 세포에서 nitric oxide (NO) 생산을 측정하였다. NL, NS, NSP는 세포 생존율에 영향을 주지 않으면서, 농도의존적으로 NO의 생산을 현저하게 저해하였으며, iNOS 및 COX-2와 같은 pro-inflammatory 중재자들의 단백질 발현을 감소시켰다. 또한, NL, NS, NSP는 MAPKs 단백질의 인신화를 감소시키고 NF-κB p65의 핵으로의 이동을 저해함으로써, 세 추출물에 의한 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 제시한다. 게다가, ROS의 생성이 세 추출물에 의해서 모두 저해되었으며, HO-1의 발현과 HO-1의 전사조절인자인 Nrf2의 핵으로의 이동이 증가되었다. 결론적으로, 이러한 연구결과는 연의 다양한 부위의 추출물인 NL, NS 그리고 NSP는 항염증 활성을 가지고 있으며, MAPKs, NF-κB, Nrf2/HO-1 등 다양한 신호경로를 통해 조절할 수 있음을 제시한다. Nelumbo nucifera, also known as sacred lotus, has mainly been used as a food throughout the Asian countries. In the present study, we prepared ethanol extracts from leaf (NL), seed (NS), and seedpod (NSP) of Nelumbo nucifera and investigated their anti-inflammatory activities in mouse macrophage RAW 264.7 cells. To evaluate the anti-inflammatory activities of NL, NS, and NSP, nitric oxide (NO) production was measured in LPS-stimulated RAW 264.7 cells. NL, NS, and NSP significantly reduced NO production in a dose-dependent manner without affecting cell viabilities. NL, NS, and NSP dramatically decreased the protein expression of pro-inflammatory genes such as iNOS and COX-2. NL, NS, and NSP also suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating they have their anti-inflammatory activities via regulating mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways. In addition, we analyzed the production of reactive oxygen species (ROS) by the treatment of NL, NS, and NSP. All extracts reduced ROS production in a dose-dependent manner. And also, they increased heme oxygenase-1 (HO-1) protein expression and the nuclear translocation of nuclear respiratory factor 2 (Nrf2). In conclusion, our results suggest that Nelumbo nucifera has its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의

김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.

Impact of the GHG Reduction on Korean Economy Using Dynamic CGE

( Chung?sil Kim ),( Sang?ho Lee ) 한국농업경제학회 2006 KOREAN JOURNAL OF AGRICULTURAL ECONOMICS(KJAT) Vol.47 No.3

This study addresses on interactions of economy and environment in a dynamic computable general equilibrium (CGE) with a focus on greenhouse gas (GHG) mitigation strategy in Korea. Whether Korea has to reduce the greenhouse gas or not, it has large influence on Korea economy, such as changes in GDP and welfare. The GDP decreases by 0.113~0.132%, and the marginal abatement costs are 110~123 $US per metric ton of CO2 for Korea. In the case of reduction of greenhouse gas in Korea, the output in fossil fuel and energy.intensive sectors are decreased sharply. Therefore, we should be exempted or delayed from the international reduction programme of greenhouse gas abatement targets within the limits of the possible.

고리원자력발전소 주변의 식생과 식물현존량 추정

김성종,이호준,김인택,김창호,정홍락 建國大學校基礎科學硏究所 1995 理學論集 Vol.20 No.-

고리원자력발전소 주변의 식생에 대한 식물사회학적 조사를 실시하였으며, 순1차생산력과 식물현존량을 추정하였다. 또한 상관에 의한 현존식생도 및 녹지자연도를 작성하였다. 종조성표에 의한 식물군락은 소나무군락, 곰솔군락, 서어나무-굴참나무군락, 아까시나무군락으로 구분되었다. 소나무군락은 아교목층(평균식피율 35%)이 빈약하였으며, 서어나무-굴참나무군락은 비교적 안정된 계층구조를 이루고 있었다. 전체적으로 교목층에서 소나무, 곰솔, 아교목층에서 사방오리, 졸참나무, 저목층에서 졸참나무, 사스레피나무, 초본층에서 산거울, 주름조개풀 등의 피복지수가 높았다. 삼림토양의 pH는 4.30~4.85, 유기물함량은 6.04~9.40%의 범위였다. 현존식생도에 의한 분포비율은 곰솔군락(31.4%)이 가장 높았으며, 평균녹지자연도는 5.4였다. Montreal model에 의한 순1차생산력은 1555.2g/㎡/yr, 식물현존량은 단위면적당16,982,21ton/k㎡으로 추정되었다. The forest vegetation and estimation of phytomass and net primary production in the vicinity of Kori Atomic Power Plant was analyzed on the basis of the phytosociological data. Depending on physiognomy, actual vegetation map and the degree of green naturality(DGN) was also drawn in the of 1:50,000. According to Z-M method, the forest vegetation was classified into four communities, which include Pinus densiflora, Pinus thunbergii, Carpinus laxiflora-Quercus variabilis, and Robinia pseudo-acacia communities. The Pinus densiflora community was most poorly covered in tree-2 layer. The Carpinus laxiflora-Quercus variabilis community was relatively stable in the stratum structure. It was generally shown that Pinus densiflora and Pinus thunbergii in tree-1 layer, Alnus firma and Quercus serrata in tree-2 layer, Quercus serrata and Eurya japonica in shrub layer, and Carex humilis and Oplismenus undulatifolius in herb layer had high coverage index. The pHs and contents of organic matter of the forest soil collected in each sites was in the range of 4.30 to 4.85 and 6.04% to 9.40% in average in the communities. The Pinus thunbergii community showed the highest distribution rate(31.4%) in the actual vegetation map. The average DGN was approximately 5.4. Net primary production and phytomass per unit area obtained by Montreal model were estimated to be 1555.2g/㎡/yr and 16,982.21ton/k㎡, respectively.

마취유도시 Thiopental Sodium과 Propanidid의 심부정맥 발생에 관한 임상적 연구

김용락,이호성,정성량,김태영,김현자,정춘근 최신의학사 1983 最新醫學 Vol.26 No.1

한국 초·중·고등학생들의 측두하악장애 유병상태와 악안면외상과의 관련성

정기호,권호근,김진범,최충호 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

The purpose of this population-based epidemiologic study was to clarify the tempromandibular disorders prevalence, the maxillofacial trauma experience rate and the relation between maxillofacial truma and tempromandibular discorders in 6-14 and 16 years old korean children who were selected through the stratified random sampling method. the results are as follows; 1. The prevalence of tempromandibular clicking sounds was 1.28% in elementary students, 11.05% in middle school students, 20.07% in high school students, with an overall 6.30%. The result of the statistical test showed that girls experienced more tempromandibular clicking sounds than boys(p<0.05). 2. The prevalence of tempromandibular pain was 0.51% in elementary students, 1.95% in middle school students, 7.04% in high school students, with an overall 20.07%. 3. The prevalence in mouth opening limitation was 0.04% in elementary students, 1.36% in middle school students, 5.85% in high school students, with an overall 1.06%. 4. The percentage of treatment experience for the tempromandibular disorders was 0.00% in elementary students, 0.77% in middle school students, 2.12% in high school students, with an overall 0.46% 5. The maxillofacial trauma experience rate was 5.45% in elementary students, 9.11% in middle school students, and 11.89% in high school students, with an overall 7.22%. Males experienced more maxillofacial trauma than females(p<0.01), and urban students experienced more than rural students)p<0.05). 6. The results of the relation betwwen maxillofacial trauma and tempromandibuar disorders showed there were statistically significant realtionship between the tempromandibular experience and the clicking sound(odds ratio 1.86), and between the maxillofacial trauma and tempromandibular disorders(odds ratio 1.79).

林土의 有機物의 分解速度와 無機養料의 垂直分布에 關한 考察

金遵敏,張楠基,鄭玩鎬 서울대학교 교육대학원 학술연구회 1966 論文集 - 서울대학교 교육대학원 Vol.3 No.-

Kim Choon Min, Chang Nam Kee and Chung Whan Ho: Decomposition rate of plant residue and the vertical distibution of mineral nutrients in the woodland soil. The relationship between decomposition rate of litter and the amount of mineral nutrients dispersed to the soil horizons were studied on the oak and pine forest stand. The results are as follows: 1. Decomposition rate of litter was highest in the F horizon of the oak stand, however in the pine stand it was highest in H horizon. 2. The correlation coefficients between decomposition rate and humus, organic carbon, moisture content, calcium, phosphorus, and nitrogen in the pine stand are; 0.90, 0.98, 0.76, 0.98, and 0.88, in oak stand; 0.97, 0.93, 0.92, 0.97, 0.74 and 0.73. 3. Factors which affect the decomposition rate of litter are humus, organic carbon, moisture content, calcium, phosphorus and nitrogen. 4. Among the variables, PH, moisture content and phosphorus under the oak and pine forest showed significant differences in each horizon. 5. C/N ratio of pine stands is higher than that of oak stands studied. 6. The vertical distribution of mineral nutrients is highly related with the amount of humus accumulated on top soil.

Platycodon grandiflorum root-derived saponins attenuate atopic dermatitis-like skin lesions via suppression of NF-kB and STAT1 and activation of Nrf2/ARE-mediated heme oxygenase-1

( Jae Ho Choi ),( Sun Woo Jin ),( Eun Hee Han ),( Bong Hwan Park ),( Hyung Gyun Kim ),( Tilak Khanal ),( Yong Pil Hwang ),( Minh Truong Do ),( Hyun Sun Lee ),( Young Chul Chung ),( Hee Suk Kim ),( Tae 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Purpose: The consequences of precipitously rising allergic skin inflammation rates worldwide have accelerated the risk of atopic dermatitis (AD). Natural product-based agents with good efficacy and low risk of side effects offer promising prevention and treatment strategies for inflammation-related diseases. We have already reported that Platycodon grandiflorum root-derived saponins (Changkil saponins, CKS) have many pharmacological effects, including anti-inflammatory and anti-allergic effects, but its influence on AD remains unclear. Therefore, we evaluated the inhibitory effect of CKS, mainly platycodin D, on AD-like skin symptoms in mice and the possible mechanisms in cells. Methods: Mice were sensitized and challenged with 2,4-dinitrochlorobenzene (DNCB). Four weeks after challenge, mice were treated with oral administration of CKS for 4 weeks. In addition, cells were used to evaluate the effect of CKS, mainly platycodin D, on the TARC expression regulated mechanism. Results: CKS attenuated DNCB-induced dermatitis severity, serum levels of IgE and TARC, and mRNA expression of TARC, TNF-α, IFN-γ, IL-4, IL-5, and IL-13 in mice. Histopathological examination showed reduced thickness of the epidermis/dermis and dermal infiltration of inflammatory cells and mast cells in the ears. Moreover, CKS and platycodin D inhibited TNF-α/IFN-γ-induced TARC expression through the suppression of NF-κB and STAT1 and induction of Nrf2/ARE-mediated hemeoxygenase-1 (HO-1) expression in cells. Conclusion: We suggest that CKS and platycodin D inhibited the development of AD-like skin symptoms by regulating cytokine mediators and may be an effective alternative therapy for AD-like skin symptoms.ⓒ2014 Elsevler Gmbh. All rights reserved.