Autophagic flux analysis of Arabidopsis seedlings exposed to salt stress

정혜라,김정훈,신광덕,김지미,이한님,정태준 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2

In plant cells, autophagy is required for efficient recycling of cytoplasmic macromolecules in vacuoles. It was previously shown that autophagy-deficient mutants also exhibited hypersensitivity to various abiotic stresses, such as salt, osmotic changes, heat, drought, and oxidative damage. However, it has not been clearly determined whether autophagy is induced or inhibited by these environmental stressors. Using the GFP-ATG8 (green fluorescent protein fused to AUTOPHAGY-RELATED PROTEIN 8) processing assay and confocal microscopy, we assessed autophagic flux of Arabidopsis seedlings exposed to salt stress. Treatment with 150 mM NaCl resulted in an increase in the processing of GFP-ATG8. Notably, the effects of concanamycin A, an inhibitor of vacuolar proton pumps, on GFP-ATG8 processing indicated that the apparent increase in GFP-ATG8 processing by salt-induced stress was due to inefficient vacuolar degradation of the GFP moiety processed from GFP-ATG8. Salt and osmotic stresses did not increase the abundance of autophagic vesicles in the root cells. Although NaCl, KCl, and mannitol did not greatly inhibit the vacuolar trafficking of GFP-ATG8, LiCl partially inhibited autophagy. These data indicated that NaCl stress neither increases nor substantially inhibits autophagic flux. Our work illustrates the importance of autophagic flux analysis to assess the effect of abiotic stresses on plant autophagy.

Cavernous Hemangioma of the Uterus in a Postmenopausal Woman - A Case Report -

정혜라,조치흠,권상훈,권선영 대한병리학회 2011 Journal of Pathology and Translational Medicine Vol.45 No.5

Cavernous hemangioma of the uterus is an uncommon mesenchymal tumor. Most cases have been reported in young, pregnant women and the condition is very rare in a postmenopausal patient. An 81-year-old woman presented with a huge pelvic mass. Abdominal computed tomography and magnetic resonance imaging results suggested a leiomyoma with degenerative change and hemorrhage. Microscopically, large, thick-walled and variable-sized vascular channels were evident in the majority part of myometrium; the lining cells were immunohistochemically reactive for CD31. Vascular tumors of the female genital tract should be cautiously excised due to the profuse intra-operative bleeding. The pathological examination of a hysterectomy specimen is the only method to confirm the diagnosis of this tumor.

심부 나보트낭 -1예 보고-

정혜라,강유나,권선영,오훈규 대한병리학회 2004 Journal of Pathology and Translational Medicine Vol.38 No.4

Herein, a case of nabothian cysts, extending into the deep cervical wall is reported. The radiologic and clinical examinations were suggestive of adenoma malignum. The cysts were incidentally found in a 42 year-old woman with abnormal uterine bleeding of about ten days duration. The patient underwent a total abdominal hysterectomy. A gross examination of the cervix revealed multiple mucin-filled cysts that extended almost to the serosa. On microscopic examination, the cysts were lined by columnar to flattened endocervical type cells, which are usually found in nabothian cysts. No atypical features or mitotic activity were noted. A deep nabothian cyst is a rare benign lesion of the cervix that should be distinguished from a cystically dilated adenoma malignum.

Apocrine Carcinoma of the Axilla Associated with Extramammary Paget’s Disease: A Case Report and Review of the Literature

정혜라,권선영,손대구 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.6

Bone marrow metastasis of small cell carcinoma of the lung mimicking Burkitt lymphoma/leukemias

정혜라,전동석,박건욱,하정숙 대한혈액학회 2011 Blood Research Vol.46 No.2

a

이주민, 인종차별과 혐오의 대상이 되다 : 미디어 속의 이주민

정혜실 우리신학연구소 2018 가톨릭 평론 Vol.16 No.-

기관지천식 환자에서 소변내 leukotriene E4 측정의 의의

정혜리(H . L . Chung) 대한천식알레르기학회 2001 천식 및 알레르기 Vol.21 No.4

N/A

혈청 IgE의 임상적 의의

정혜리 대한소아청소년과학회 2007 Clinical and Experimental Pediatrics (CEP) Vol.50 No.5

Many previous studies have proved that human allergic disease resulted from the formation of antibodies belonging to a unique immunoglobulin isotype termed immunoglobulin E (IgE). Most of IgE- producing plasma cells are found in the lymphoid tissue associated with the gastrointestinal and respiratory tracts. IgE may be found free in the mucosal secretions of these tissues, bound to local mast cells, or distributed by the systemic circulation to mast cells and basophils throughout the body. Total serum IgE concentrations tend to be higher in allergic adults and children compared with non-allergic individuals, but the value of total serum IgE as a screening test for allergic disease is limited. Total serum IgE levels are related to the probability of an individual having detectable allergen-specific IgE. Allergen-specific IgE concentrations vary with a person's age, the degree and duration of the recent allergen or cross-reactive allergen exposure. The value of quantitative assays for allergen-specific IgE has been suggested in recent studies. Serum IgE increases in many non-allergic diseases, including infectious and parasitic diseases. The IgE changes appear to be specific to the infectious agents, whereas non-specific in other diseases. The increased serum IgE in some of these conditions probably results from alterations in immune function. This review summarizes the clinical significance of total and allergen-specific IgE examinations in allergic diseases.

Initial Experience with Magnetic Resonance-Guided Vacuum-Assisted Biopsy in Korean Women with Breast Cancer

정혜나,한부경,고은영,신정희 한국유방암학회 2014 Journal of breast cancer Vol.17 No.3

Purpose: The aim of this study is to describe our initial experiencewith magnetic resonance (MR)-guided biopsy and to determinethe malignancy rate of additional lesions identified by MR only inKorean women with breast cancer. Methods: A retrospective reviewidentified 22 consecutive patients with breast cancer whohad undergone MR-guided vacuum-assisted biopsies (VAB) ofMR-only identified lesions from May 2009 to October 2011.Weevaluated the rate of compliance, the technical success for MRguidedVAB and the MR imaging findings of the target lesions. VAB histology was compared with surgical histology and followupimaging findings. Results: The biopsy recommendations forMR-only identified lesions were accepted in 46.8% (22/47) ofpatients. One of 22 procedures failed due to the target’s posteriorlocation. Among 21 MR-guided VAB procedures, the target lesionswere considered as a mass in 12 cases and a nonmassenhancement in nine cases. VAB histology revealed malignanciesin 14% (3/21) of cases, high-risk lesions in 24% (5/21) andbenign lesions in 62% (13/21). Eleven cases (52%, 11/21) had apositive surgical correlation, and one of them was upgraded fromatypical ductal hyperplasia to invasive ductal carcinoma. In theremaining 10 lesions, follow-up breast ultrasound and mammographywere available (range, 15–44 months; mean, 32.1 months)and did not show suspicious lesions. The final malignancy ratewas 19% (4/21). Conclusion: MR-guided VAB for MR-only identifiedlesions yielded a 19% malignancy rate in Korean womenwith breast cancer. MR-guided VAB helps surgeons avoid an unnecessarywide excision or additional excisional biopsy.

Effectiveness of the Use of Standardized Vocabularies on Epilepsy Patient Cohort Generation

정혜실,이호영,유수영,황희,백현영 대한의료정보학회 2022 Healthcare Informatics Research Vol.28 No.3

Objectives: This study investigated the effectiveness of using standardized vocabularies to generate epilepsy patient cohortswith local medical codes, SNOMED Clinical Terms (SNOMED CT), and International Classification of Diseases tenth revision(ICD-10)/Korean Classification of Diseases-7 (KCD-7). Methods: We compared the granularity between SNOMED CTand ICD-10 for epilepsy by counting the number of SNOMED CT concepts mapped to one ICD-10 code. Next, we createdepilepsy patient cohorts by selecting all patients who had at least one code included in the concept sets defined using eachvocabulary. We set patient cohorts generated by local codes as the reference to evaluate the patient cohorts generated usingSNOMED CT and ICD-10/KCD-7. We compared the number of patients, the prevalence of epilepsy, and the age distributionbetween patient cohorts by year. Results: In terms of the cohort size, the match rate with the reference cohort was approximately99.2% for SNOMED CT and 94.0% for ICD-10/KDC7. From 2010 to 2019, the mean prevalence of epilepsy definedusing the local codes, SNOMED CT, and ICD-10/KCD-7 was 0.889%, 0.891% and 0.923%, respectively. The age distributionof epilepsy patients showed no significant difference between the cohorts defined using local codes or SNOMED CT, but theICD-9/KCD-7-generated cohort showed a substantial gap in the age distribution of patients with epilepsy compared to thecohort generated using the local codes. Conclusions: The number and age distribution of patients were substantially differentfrom the reference when we used ICD-10/KCD-7 codes, but not when we used SNOMED CT concepts. Therefore, SNOMEDCT is more suitable for representing clinical ideas and conducting clinical studies than ICD-10/KCD-7.