쥐 해마의 Lipopolysaccharide 국소주입에 의한 Tumor Necrosis Factor-α, Interleukin-1β 및 Inducible Nitric Oxide Synthase의 발현

오훈규,강구성,김지연,곽은경,김정완,박지영,손윤경 대한병리학회 2004 Journal of Pathology and Translational Medicine Vol.38 No.3

Background : Brain inducible nitric oxide synthase (iNOS) might be detectable in several pathologic conditions, and it is thought to play an important role in their pathophysiology. Tumor necrosis factor (TNF)- and interleukin (IL)-1 are believed to be essential factors of iNOS induction of the brain. Methods : After intrahippocampal stereotaxic injection of lipopolysaccharide (LPS), the rat brains were removed at 6, 12 and 24 h. The rat brain tissues were examined to clarify the expression patterns of TNF- , IL-1 and iNOS. Results : The inflammatory cells which were stained with anti-TNF- antibody, appeared in 6 h and increased for 24 h after LPS injection. The iNOS positive cells appeared after 12 h of LPS injection. A semiquantitative analysis of reverse transcription-polymerase chain reaction (RT-PCR) revealed that the TNF- and IL-1 mRNA arose at 1 h, peaked at 6 h and then declined until 48 h after LPS injection. The iNOS mRNA arose after 6 h, peaked at 12 h, and declined until 48 h after LPS injection. Conclusions : We conclude that the induction of inflammatory events by intrahippocampal injection of LPS activates TNF- and IL-1 secretion, and this is followed by an induction of iNOS expression. TNF- and IL-1 seem to be related with iNOS expression in brain inflammation.

두개저의 혈관확장형 골육종 1례

오훈규,김용진 영남대학교 의과대학 1999 Yeungnam University Journal of Medicine Vol.16 No.2

A rare case of telangiectatic osteosarcoma of the sphenoid bone was reported. The patient was a 27-year-old male and had suffered from left eye protrusion and diplopia for three months. Radiologically, a lobulated osteolytic lesion was located between sphenoid and left temporal bone. Pathologically, the tumor had multiloculated blood filled cystic vascular spaces and osteoid formation by malignant spindle osteoblast cells. The differential diagnosis of this rare entity from aneurysmal bone cyst was important radiologically and pathologically. This case is the second case of telangietatic osteosarcoma of the sphenoid bone followed by a case of Whitehead RE and Melhem ER in 1998.

장간막 이소골화 - 1예 보고 -

오훈규,배종엽 대한병리학회 2006 Journal of Pathology and Translational Medicine Vol.40 No.1

Heterotopic mesenteric ossification is a very rare reactive lesion in the small bowel mesentery, and it is related with trauma or surgical operation. It is pathologically characterized by well formed bone trabeculae and prominent osteoblastic rimming and is clinically related to rapid and recurrent bowel obstruction symptoms. This unusual reactive process shares many clinical and pathologic features with myositis ossificans. We report here on a rare case of heterotopic mesenteric ossification in 28-year-old man who underwent a delayed small bowel resection 15 days after trauma.

거짓상피종증식을 동반한 코형 NK/T세포림프종 - 1예 보고 -

오훈규,김정규,박관규 대한병리학회 2006 Journal of Pathology and Translational Medicine Vol.40 No.3

A case of prominent pseudoepitheliomatous hyperplasia (PEH), that was misdiagnosed as squamous cell carcinoma (SCC) on the frozen section occured in the nasal mucosa of a patient suffering with nasal type NK/T cell lymphoma. To prevent misdiagnosis of this lesion, pathologists should be aware that NK/T cell lymphoma may be associated with overlying mucosal PEH, and so the physician must adhere to strict diagnostic criteria for making the diagnosis of SCC. The pathogenesis of PEH associated with NK/T cell lymphoma is not still clear, but it may be related to the production of growth factors, especially epidermal growth factor and transforming growth factor, by the underlying tumors.

구불창자에서 발생한 횡문근모양 샘암종- 1예 보고 -

오훈규,조창호,금윤섭 대한병리학회 2008 Journal of Pathology and Translational Medicine Vol.42 No.1

Colorectal adenocarcinoma with rhabdoid features is extremely rare and only two cases have been previously reported. We report here on a case of colorectal adenocarcinoma with prominent rhabdoid features in a 69-year-old female. The specimen was an ulcerative mass from the sigmoid colon, and it measured 3.5×3 cm. Microscopic examination of the tumor showed mostly rhabdoid cells that had eccentrically located large nuclei and foci of glandular formation. A transitional area from the poorly differentiated adenocarcinoma to the rhabdoid tumor was also noted. Immunohistochemical studies showed strong reactivity of the glandular forming cells for pan-cytokeratin, and the cells were occasionally positive for vimentin. The cells with rhabdoid features were diffusely positive for vimentin and focally positive for pan-cytokeratin. These results suggested that the cells with rhabdoid features originated from dedifferentiated primary adenocarcinoma. Since colorectal adenocarcinoma with rhabdoid features is highly aggressive and unresponsive to conventional therapy, making the preoperative diagnosis is important to facilitate the treatment.

악성중피종 환자의 폐 조직에서의 석면체 분석 -1예 보고-

오훈규,노재요,윤철종,지제근 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.5

FGS/NgaKist 백서신장의 형태학적 및 변환 성장인자-β의 변화

오훈규,김용진,박미옥,이철호,현병화,서인수 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.1

Overexpression of CD73 in epithelial ovarian carcinoma is associated with better prognosis, lower stage, better differentiation and lower regulatory T cell infiltration

오훈규,신정임,최정혜,박성혜,이태성,최윤석 대한부인종양학회 2012 Journal of Gynecologic Oncology Vol.23 No.4

Objective: The purpose of the current study was to evaluate survival outcome according to the expression status of CD73 in patients with epithelial ovarian cancer. Methods: A total of 167 patients with epithelial ovarian cancer were enrolled in the current study. For each patient, a retrospective review of medical records was conducted. Immunohistochemical staining for CD73, CD8, FoxP3, and CD68 was performed using tissue microarray made with paraffin embedded tissue block. Results: Among the enrolled patients, 29.9% of patients (n=50) showed negative expression for CD73, whereas 70.1% of patients (n=117) showed positive expression for CD73. The CD73 positive group showed better prognosis compared to the CD73 negative group (5-year overall survival of CD73 positive group, 73.0%; that of CD73 negative group, 50.1%; p=0.023). CD73 was more frequently expressed in mucinous adenocarcinoma and clear cell carcinoma compared to serous or endometrioid adenocarcinoma. In addition, CD73 overexpressions were more frequently detected in patients with known good prognostic factors, i.e., low stage, well/moderate differentiation, negative peritoneal cytology, no lymphovascular involvement, and no macroscopic residual tumor after debulking surgery. There was significantly more infiltration of regulatory T cells in the CD73 negative group compared to the CD73 positive group. Conclusion: Good prognosis in patients with overexpression of CD73 may be due to that overexpression of CD73 was more frequently observed in epithelial ovarian cancer patients with known good prognostic factors. Therefore, this result means that favorable differentiation and stage have more influence on survival outcome than adverse effect of CD73 per se. Objective: The purpose of the current study was to evaluate survival outcome according to the expression status of CD73 in patients with epithelial ovarian cancer. Methods: A total of 167 patients with epithelial ovarian cancer were enrolled in the current study. For each patient, a retrospective review of medical records was conducted. Immunohistochemical staining for CD73, CD8, FoxP3, and CD68 was performed using tissue microarray made with paraffin embedded tissue block. Results: Among the enrolled patients, 29.9% of patients (n=50) showed negative expression for CD73, whereas 70.1% of patients (n=117) showed positive expression for CD73. The CD73 positive group showed better prognosis compared to the CD73 negative group (5-year overall survival of CD73 positive group, 73.0%; that of CD73 negative group, 50.1%; p=0.023). CD73 was more frequently expressed in mucinous adenocarcinoma and clear cell carcinoma compared to serous or endometrioid adenocarcinoma. In addition, CD73 overexpressions were more frequently detected in patients with known good prognostic factors, i.e., low stage, well/moderate differentiation, negative peritoneal cytology, no lymphovascular involvement, and no macroscopic residual tumor after debulking surgery. There was significantly more infiltration of regulatory T cells in the CD73 negative group compared to the CD73 positive group. Conclusion: Good prognosis in patients with overexpression of CD73 may be due to that overexpression of CD73 was more frequently observed in epithelial ovarian cancer patients with known good prognostic factors. Therefore, this result means that favorable differentiation and stage have more influence on survival outcome than adverse effect of CD73 per se.

간성상세포의 활성에서 siRNA를 이용한 TGF-β1의 전사조절

오훈규,김경현,금윤섭,조창호,박재복,박관규 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.6

Background : The cytokine-induced activation of hepatic stellate cells (HSC) plays a major role in liver fibrosis. Quiescent HSCs undergo phenotypic transformation called “transdifferentiation” in response to viral, chemical or immune insults to the liver. The cytokine TGF-β1 plays a key role in progressive liver fibrosis. Since small interfering RNA (siRNA) is a powerful tool for silencing gene expression post-transcriptionally, the present study aimed to determine whether synthetic TGF-β1 siRNA down-regulates the expression of the TGF-β1 gene in immortalized and activated rat HSCs (HSC-T6s). The study examined whether synthetic TGFβ1 siRNA prevents rat HSCs activation and extracellular matrix (ECM) production. Methods : TGF-β1 siRNA or a control (pU6) siRNA was added to HSC-T6 culture media. We then performed RT-PCR and western blot analyses for TGF-β1 and ECM components (fibronectin, type-I collagen, and TIMP-1). Results : TGF-β1 siRNA significantly down-regulated expression of TGF-β1 mRNA and protein and attenuated mRNA and protein expressions of type-I collagen, fibronectin, and TIMP-1, as compared to the control. Conclusions : TGF-β1 siRNA can effectively down-regulate the expression of TGF-β1 in rat HSC, resulting in significant inhibition of HSC activation and of ECM production. These data indicate that synthetic TGF-β1 siRNA can be a useful treatment modality to prevent liver fibrosis. Background : The cytokine-induced activation of hepatic stellate cells (HSC) plays a major role in liver fibrosis. Quiescent HSCs undergo phenotypic transformation called “transdifferentiation” in response to viral, chemical or immune insults to the liver. The cytokine TGF-β1 plays a key role in progressive liver fibrosis. Since small interfering RNA (siRNA) is a powerful tool for silencing gene expression post-transcriptionally, the present study aimed to determine whether synthetic TGF-β1 siRNA down-regulates the expression of the TGF-β1 gene in immortalized and activated rat HSCs (HSC-T6s). The study examined whether synthetic TGFβ1 siRNA prevents rat HSCs activation and extracellular matrix (ECM) production. Methods : TGF-β1 siRNA or a control (pU6) siRNA was added to HSC-T6 culture media. We then performed RT-PCR and western blot analyses for TGF-β1 and ECM components (fibronectin, type-I collagen, and TIMP-1). Results : TGF-β1 siRNA significantly down-regulated expression of TGF-β1 mRNA and protein and attenuated mRNA and protein expressions of type-I collagen, fibronectin, and TIMP-1, as compared to the control. Conclusions : TGF-β1 siRNA can effectively down-regulate the expression of TGF-β1 in rat HSC, resulting in significant inhibition of HSC activation and of ECM production. These data indicate that synthetic TGF-β1 siRNA can be a useful treatment modality to prevent liver fibrosis.

양수색전증 부검 1 예

임재원,곽정식,채종민,오훈규 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

We experienced an autopsy case of sudden and unexpected natural death due to disseminated intravascular coagulopathy secondary to amniotic fluid embolism. The underlying cause is the infusion of amniotic fluid with all of its contents into the maternal circulation following a tear in the placental membranes and rupture of uterine and/or cervical veins. As a consequence, epithelial squames from fetal skin, lanugo hair, fat from vervix caseosa, mucin from the fetal respiratory or gastrointestinal tract, and occasionally bile from meconium contamination of amniotic fluid can be found in the victim's pulmonary microcirculation at postmortem examination. fortunately, amniotic fluid embolism is uncommon, with an incidence of about 1 per 50,000 deliveries, but it incurs a mortality rate of 86%. The clinical presentation is striking-suddenly and without warning, profound respiratory difficulty with deep cyanosis and cardiovascular shock appear, followed rapidly in some cases by clonic-tonic convulsions and profound coma. In about half the cases, Excessive bleeding from the uterus and birth canal is attributeed to the development of DIC due to release of thromboplastic substances in amniotic fluid?.