석고의 생쥐 비장세포 인터루킨-4 분비에 미치는 효과

임강현,김성우,김현미,김정선,조정제,유영천,안현종 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

구척이 흰쥐의 장골 길이성장에 미치는 영향

임강현,김호철 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

In this study, we searched some herbal medicines on promoting the heights of longitudinal bone and found that the root of Cibotium barometz promotes longitudinal bone growth. The longitudinal bone growth was measured in the proximal tibia by fluorescence microscope with tetracycline. Tetracycline plays a chelate with calcium ion which is accumulated in bone forming-zones. The accumulated tetracycline made fluorescent lines corresponding to two injections. The growth rate of bones are evaluated by measuring the length between two lines. The longitudinal bone growth rate of control group for two days was 489.0±70.1 ㎛ but, C. barometz administration significantly promoted the longitudinal bone growth(100 ㎎/㎏, 764.2±41.1 ㎛, p<0.01). And the heights of proliferative and hypertrophic zones were also increased in C. barometz administered group measured by Nissl staining. To evaluate its growth promoting mechanisms, the induction of local insulin-like growth factor-1(IGF-1) and bone morphogenetic protein-2(BMP-2) were measured by using immunohistochemical method. C. barometz increased local induction of BMP-2 in hypertrophic and ossification zones, whereas IGF-1 did not increase. These results showed that administration of the extracts of C. barometz promoted longitudinal bone growth and its mechanism could be explained in part by regulation of BMP-2 induction in growth plates.

續斷의 흰쥐장골 길이성장에 미치는 영향에 관한 연구

임강현,전훈 대한동의생리학회,대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.6

In this study, we searched some herbal medicines on promoting the heights of longitudinal bone and found that the growth promotion effect of Dipsacus asper Wall. on the longitudinal bone of adolescent male rat was significantly high. The longitudinal bone growth was measured in the proximal tibia by tetracycline method. And the heights of proliferative and hypertrophic zones were also increased in D. asper administered group measured by Nissl staining. And the induction of local insulin-like growth factor-1(IGF-1) and bone morphogenetic protein-2 (BMP-2) were measured by using immunohistochemical method. D. asper increased local induction of BMP-2 in maturing and hypertrophic zones, whereas IGF-1 was slightly increased. These results showed that administration of the extracts of D. asper promoted longitudinal bone growth and its mechanism could be explained partially by regulation of IGF-1 and BMP-2 induction in growth plates.

석고의 생쥐 비장세포 인터루킨-4 분비에 미치는 효과

임강현,김성우,김현미,김정선,조정제,유영천,안현종 한약응용학회 2002 한약응용학회 학술대회 Vol.2002 No.-

Porcine Skin Gelatin Hydrolysate Promotes Longitudinal Bone Growth in Adolescent Rats

임강현,이세나,장애라,김혜경 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.5

Collagen hydrolysates (CHs) are mixtures of peptides obtained by partial hydrolysis of gelatin that are receiving scientific attention as potential oral supplements for the restoration of osteoarticular tissues. The aim of this study was to evaluate the effectiveness of CHs for promoting longitudinal bone growth in growing rats. An in vitro study was carried out in osteoblast-like MG63 cells and the most effective CH on bone formation was selected among 36 various CHs. An in vivo study confirmed the functional effects of a selected CH with molecular weight of <3 kDa on longitudinal bone growth. CHs dose-dependently promoted the longitudinal bone growth and height of the growth plate in adolescent male rats, whereas gelatin failed to affect longitudinal bone growth. Insulin-like growth factor-1 and bone morphogenetic protein-2 in the CH treated group were highly expressed in the growth plate. These results suggest that CHs isolated in this study may provide beneficial effects on bone metabolism of growing animals and humans.

주자독서환(朱子讀書丸)의 아밀로이드베타로 유발된 생쥐 알츠하이머모델에 대한 효과

임강현,고흥,경혁수,Leem, Kang-Hyun,Ko, Heung,Kyung, Hyuk-Su 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.1

Object: This research investigated effects of Jujadokseo-hwan on mice with Alzheimer's Disease induced by $amyloid-{\beta}$. According to Dongyibogam, Jujadokseo-hwan can cure amnesia. Amyloid-B is believed to induce oxidative stress and inflammation in the brain, postulated to play important roles in the pathogenesis of Alzheimer's disease. In this way $Amyloid-{\beta}$ induces Alzheimer's Disease. Methods : In order to make an efficient prescription and cope with dementia, learning and memory functions of mice were tested on passive avoidance test and V-maze task. $NF-{\kappa}B$ were measured from protein derived from the brain. RT-PCR was done for !gene analysis. Primers were protein kinase Band $NGF-{\alpha}$. Results : 1. Jujadokseo-hwan was effective for memory capacity on passive avoidance test. but noneffective for spatial memory capacity and locomotor activity on Y -maze task. 2. The measurement of $NF-{\kappa}B$ showed upward tendancies and the result of RT-PCR showed up-regulation when given Jujadokseo-hwan by mouth. Conclusion: Results suggest that Jujadokseo-hwan is effective on mice with Alzheimer's Disease induced by $amyloid-{\beta}$.

다엽(茶葉)의 4 - VO 로 유발한 흰쥐뇌허혈의 신경방어효과

林康鉉 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

Green tea(茶葉), the leaves of Camellia sinensis (L.) O. Kuntze (Thea sinensis L.), whose property, flavour and channel tropism is bitter and sweet in flavour(苦甘), cool in property(?), acting on the heart, lung, stomach and kidney channels(歸心???經), has the effects of clearing the head and eyes(淸?目), removing the thirst(???), digesting the food(?食), resolving the dampness(化?), facilitating the urination(利?), neutralizing a poison(解?). And this is also one of the most popular beverages in the world. So, this study was planned to check the neuroprotective effect of green tea on the global ischemia induced by 4-vessel occlusion in Wistar rats. Tea extract was lyophilized after extraction with water. We induced 4-vessel occlusion for 10 minutes and reperfused again. The number of CA1 pyramidal neurons were counted after 7 days of reperfusion under the cresyl violet staining. The results obtained that in 4-VO ischemia, Green tea showed significant neuroprotective effects(20 and 100 ㎎/㎏ of green tea extracts, p<0.05 and p<0.001 respectively) compared with control group. Each neuroprotective ratio was about 53.8%, 97.6% respectively. We estimated the amount of the main component (-)epigallocatechin gallate(EGCG) by HPLC, found out its net content in extract was about 9%. However, EGGG given at 10 ㎎/㎏ had HPLC, found out its net content in extract was about 9%. However, EGGG given at 10 ㎎/㎏ had no effect on global ischemia, indicating that EGGG may not be the only main active component of green tea. Immunohistochemical data partially explained the protective effects of green tea via promotion of bcl-2 induction in hippocampus. Consequently, green tea has neuroprotective effects on the global ischemia induced by 4-vessel occlusion in Wistar rats. This work was supported by Korea Research Foundation Grant. (KRF-2000-003-F00311)

Effects of Albizziae Cortex Extracts on the Elastase Activity and DPPH and NO Scavenging Activities

임강현 한의병리학회 2011 동의생리병리학회지 Vol.25 No.2

Elastic fibers are found in the skin, lungs, arteries, veins and other structures. Elastases destroy the elastic fibers and cause the emphysema and pulmonary hypertension. Oxidative stress is needed for these pathologic changes. Accordingly, present study was designed to investigate the effect of Albizziae Cortex extracts (ACE) on elastase activity and anti-oxidative effects of ACE. The in vitro inhibitory effects on elastase and di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) and nitric oxide (NO) free radical scavenging activities of ACE were measured. The elastase activity was significantly inhibited by ACE. DPPH and NO free radicals were significantly scavenged as well. ACE showed the elastase-inhibiting effects and anti-oxidative activities in vitro. These results suggest that ACE may have potential roles in the treatment of pulmonary emphysema and pulmonary hypertension

Thiopental sodium으로 유도된 수면에서 황련아교탕의 입면 및 수면지속 효과

임강현,김태연 한의병리학회 2022 동의생리병리학회지 Vol.36 No.1

Hwangryunagyo-tang (HT) is a herbal cocktail to treat insomnia induced by yin deficiency with effulgent fire. In the present study, the onset time and the duration time of sleep were measured 30 minutes after thiopental sodium injection. And c-fos immunohistochemistry was performed to find the activated nerve cells of the ventrolateral preoptic nucleus (VLPO) and tuberomammillary nucleus (TMN) sites in the brain. HT significantly increased the number of activated nerve cells in the sleep-inducing center (VLPO), whereas HT significantly decreased the number of activated nerve cells in the arousal center (TMN). It could be concluded that the HT shortened the onset time and increased the duration time for sleep induced by thiopental sodium. And it was confirmed that the mechanism acted by activating the sleep-inducing center (VLPO) and suppressing the arousal center (TMN) in the brain. The results are considered to be useful as scientific evidence HT can be used clinically for the treatment of insomnia caused by yin deficiency with effulgent fire.

전통한의학으로부터 소아성장촉진 바이오소재 개발연구

임강현 충북연구원 2003 충북발전연구원 학술세미나 Vol.- No.-