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임현,남주원,서은경,Yeong Shik Kim,김현표 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.11
To assess the anti-inflammatory activity of constituents from the rhizomes of Anemarrhena asphodeloides, (-)-nyasol {cis-hinokiresinol, 4,4-[1Z,3R]-3-ethenyl-1-propene-1,3-diyl]bisphenol} was isolated and its anti-inflammatory activity was examined in lipopolysaccharide (LPS)-treated RAW 264.7 cells and A23187-treated RBL-1 cells. In vivo activity was measured using carrageenan-induced paw edema assay. At > 1 μM, (-)-nyasol significantly inhibited cyclooxygenase-2 (COX-2)-mediated PGE2 production and inducible nitric oxide synthase (iNOS)-mediated NO production in LPS-treated RAW 264.7 cells, a mouse macrophage-like cell line, but did not affect the expression levels of COX-2 and iNOS. (-)-Nyasol also inhibited 5-lipoxygenase (5-LOX)-mediated leukotriene production in A23187-treated RBL-1 cells. Furthermore, (-)-nyasol potently inhibited carrageenan-induced paw edema in mice (28.6-77.1% inhibition at 24-120 mg/kg). Therefore, (-)-nyasol is a potential new lead compound and may contribute to the anti-inflammatory action of A. asphodeloides, possibly by inhibiting COX-2, iNOS and 5-LOX.
Separation of two major chalcones from Angelica keiskei by high-speed counter-current chromatography
길윤서,남주원,이준,서은경 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.8
Angelica keiskei (Shin-sun cho) is an edible higher plant with the beneficial preventive effects on cancer, hypertension, and coronary heart disease. Two bioactive chalcones of Shin-sun cho, xanthoangelol (1) and 4-hydroxyderricin (2), were separated simultaneously by using high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane–EtOAc– MeOH–H2O (9:5:9:4). Only nonconsuming processes, solvent fractionations and Sephadex LH-20 column chromatography, were conducted as presteps. Xanthoangelol (1, 35.9 mg, 99.9 % purity at 254 and 365 nm) and 4-hydroxyderricin (2, 4.4 mg, 98.7 % purity at 254 nm and 98.8 % purity at 365 nm) were successfully purified from 70 mg of the processed extract from A. keiskei. The structures of two compounds were confirmed by 1H- and 13C-NMR analysis.
Isolation and Structural Identification of Minor Constituents from Sasa borealis
Yeon Hee Jeong,,남주원,Na Youn Lee,,서은경,Youngjoo Kwon 한국생약학회 2005 Natural Product Sciences Vol.11 No.3
Compounds of (+)-5,5'-dimethoxylariciresinol (1) and (3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one (2 Sasa borealis (Hack.) Makino (Gramineae)for the first time in the present investigation. The structures of compounds were identified by analysis of spectraldata including 1D- and 2D-NMR spectra as well as by comparison of their data with the published values. Thesecompounds have never been isolated previously from the family Gramineae.
Minor Phenolic Constituents of the Anemarrhenae Rhizoma
윤의중,Ye Seul Lee,Hana Jeong,남주원,Yoo Jin Lee,Young Min Son,황은숙,서은경 한국생약학회 2009 Natural Product Sciences Vol.15 No.4
A homoisoflavanone, 7,4'-dihydroxyhomoisoflavanone (1) and a flavanone, (2S)-7,4'-dihydroxy-5- methoxyflavanone (2), were isolated from the rhizomes of Anemarrhena asphodeloides, together with 4,4'- dihydroxychalcon (3), 2'-O-methylphlorethin (4), 1,3-bis-di-p-hydroxyphenyl-4-penten-1-one (5), and 2,4'- dihydroxy-4-methoxybenzophenone (6) on the basis of spectroscopic and physicochemical analyses including 1Dand 2D- NMR techniques as well as by comparison of their data with the published values. Compounds 1 - 4 were isolated for the first time from this plant source. Among isolates, compound 2 exhibited moderate inhibitory effect on the differentiation of pre-adipocyte 3T3-L1 cells.
김금진,이종영,최현규,김소영,김언미,심상희,남주원,김상현,최혁재 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.3
Cinnamomum cassia (Lauraceae) has long beenused as one of the most frequently used traditional orientalmedicines for the treatment of gastritis, diabetes, bloodcirculation disturbance and inflammatory diseases. Cinnamomulactone(1), a new butyrolactone was isolated fromthe twigs of C. cassia together with nine known compounds,coumarin (2), trans-cinnamic acid (3), cinnamaldehyde (4),2-hydroxycinnamaldehyde (5), 2-methoxycinnamaldehyde(6), 2-hydroxy-cinnamyl alcohol (7), benzoic acid (8),(?)-syringaresinol (9) and phenethyl (E)-3-[4-methoxyphenyl]-2-propenoate (10). The planar structure of 1 waselucidated on the basis of spectroscopic data analysis and itsconfigurations were determined by coupling constant (3JHH)analysis and a comparison with specific rotation data ofrelated compounds on the literatures. The structures ofknown compounds were confirmed by the comparison oftheir spectroscopic data to the reported values. Compound10 was isolated for the first time from this plant. Compounds1, 2, 4, and 9 showed inhibitory activity againstmatrix metalloproteinases (MMPs) gene expression. Among them, compound 1 has been revealed to suppressthe gene expression of MMP-3 and interleukin (IL)-1b aswell as MMP-1 in tumor necrosis factor (TNF)-a stimulatedrheumatoid arthritis synovial fibroblasts.
Efficacy of Veronica incana for Treating Osteoarthritis Induced by Monosodium Iodoacetate in Rats
이진아,Trung Huy Ngo,신유옥,최정원,최혁재,남주원,노성수 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.6
The aim of this study is to investigate the efficacy and the underlying mechanism of Veronica incana in osteoarthritis (OA) induced by intraarticular injection of monosodium iodoacetate (MIA). The selected major four compounds (A–D) of V. incana were found from fractions 3 and 4. Its structure elucidation was determined by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) data analysis and nuclear magnetic resonance (NMR) data comparison with literature. MIA (50 μL with 80 mg/mL) for the animal experiment was injected into the right knee joint. The V. incana was administered orally every day to rats for 14 days from 7 days after MIA treatment. Finally, we confirmed the four compounds: (A) verproside; (B) catalposide; (C) 6-vanilloylcatapol; and (D) 6-isovanilloylcatapol. When we evaluated the effect of V. incana on the MIA injection-induced knee OA model, there were a noticeable initial decreased in hind paw weight-bearing distribution compared to the Normal group (P < .001), but V. incana supplementation resulted in a significant increase in the weight-bearing distribution to the treated knee (P < .001). Moreover, the V. incana treatment led to a decrease in the levels of liver function enzymes and tissue malondialdehyde (P < .05 and .01). The V. incana significantly suppressed the inflammatory factors through the nuclear factor-kappa B signaling pathway and downregulated the expression of matrix metalloproteinases, which are involved in the degradation of the extracellular matrix (P < .01 and .001). In addition, we confirmed the alleviation of cartilage degeneration through tissue stains. In conclusion, this study confirmed the major four compounds of V. incana and suggested that V. incana could serve as an anti-inflammatory candidate agent for patients with OA.