소아의 치면세균막에 존재하는 mutans streptococci의 분포

국중기,박종휘,유소영,김화숙,이난영 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.3

한국인 소아의 치면세균막에 존재하는 mutans streptococci 종 및 생물형의 발현빈도와 치아우식경험지수와 상관관계를 알아보기 위하여. 조선대학교 치과병원에 내원한 12세 미만 113명의 소아 환자의 치아우식경험지수를 구하고, 이들의 상하악 유전치 및 유구치의 협면 및 설면의 치면세균막 샘플을 채취하여 mutans streptococci를 mitis-salivarius bacitracin 배지에서 선택적으로 분리하였다. 이들의 biotype을 알아보기 위해 생화학적 검사를 실하였고, 이들의 종 수준에서의 동정을 위해 dextranase 유전자를 표적으로 하는 중합효소연쇄반응을 시행하여 하였다. 113명의 환자 중에서 40명의 치면세균막에서 40 균주의 mutans streptococci이 검출되었다. 이들 중 생물형 제 Ⅰ형 (45%)이 가장 많이 검출되었으며, 그 다음으로 제Ⅳ형 (32.5%), 제 Ⅱ형 (15%). 제 Ⅴ형 (5%), 제 Ⅲ형 (2.5%) 순으로 검출되었다. 또한, 종 수준에서의 발현빈도를 알아본 결과 S. mutans가 69%, S. sobrinus는 31%였다. Mutans streptococci 종 또는 생물형에 따른 환자의 치아우식경험지수간의 차이는 없는 것으로 조사되었다(p>0.05). 이상의 결과를 종합할 때, 한국인의 소아의 구강 내에 존재하는 mutans streptococci중 생물형 제 Ⅰ형인 S. mutans가 가장 높은 빈도로 존재하며, 치아우식증이 세균학적 요소만이 아닌 기타 여러원인 요소에 의해 발병되는 다는 여러 연구 결과와 일치함을 알 수 있었다. The aim of this study is to survey the frequency of mutans streptococci species and biotypes isolated from dental plaque in Korean children and the relationship between species and biotypes of mutans streptococci and dft index. Dental plaques were collected from the anterior and molar teeth of upper and lower jaws in the subjects, aged below 12 years old. A dental examination was performed for dft (decayed, filed, total) with the WHO caries diagnostic criteria. The mutans streptococci from the sample were cultured selectively on mitis salivarius-bacitracine (MSB) agar plate. For biotyping of mutans streptococci, biochemical test was performed. From the culture, bacterial genomic DNA was prepared f3r using of PCR template for the identification of mutans streptococci at the species-level. Forty strains of mutans streptococci were isolated from dental plagues of 40 patients. The biotype Ⅰ (45%) and biotype Ⅳ (32.5%) were most frequently detected. The prevalence of s. mutans and S. sobrinus was 69% and 31%, respectively There was no positive relationship between species and biotypes of mutans streptococci and dft index. Our results revealed that biotype Ⅰ and S. mutans were frequently detected in Korean children and support that dental caries incidents by many causative factors not only bacterial factor.

보건소 구강보건실 활성화를 위한 기초구강보건실태조사 사업사례

국중기,강현식,마재완,김진수,김학주,정병초,정성국,윤태원,고경숙,김선심,김은화,명연자,배민진,송내옥,오금란,윤성숙,이연옥,임은경,이덕순,박인찬,서병종,김동기 대한구강보건학회 1997 大韓口腔保健學會誌 Vol.21 No.5

시간경과에 따른 치면열구전색체의 외형변화와 치아우식발생과의 관계

국중기,양정승,임선아,성진효,김동기 대한구강보건학회 2001 大韓口腔保健學會誌 Vol.25 No.4

We conducted dental sealant programme as a public oral health program in elementary school children to prevent occlusal caries and to improve oral health. Subjected teeth were 337 pairs of sound first molars. We checked and discribed the pattern of outline change of sealants and caries incidence every 3-month for 30 months. The aim of this study was to evaluate the relation between outline change of sealant body and caries incidence. Obtained results were as follows ; 1. D-type loss of sealants was most frequent and highest caries incidence. 2. The rate of sealant loss including partial loss and caries incidence was rapidly increased between 9th and 12th month and between 24th and 17months. 3. In light-cured sealants, the rate of sealant loss was lower and caries preventive effectiveness was higher as compared with self-cured sealants. 4. The rate of sealants loss in warm drying method was slightly lower and caries preventive effectiveness was higher than cold drying method. From these results, we suggested that considering the type of sealant loss, periodic reapplication of sealants every 1-year was very important in order to maintain higher caries preventive effectiveness.

치면세균막에서 분리한 뮤탄스 연쇄상구균 및 Streptococcus anginosus의 수종 항생제에 대한 감수성 조사

국중기,임상수,유소영,황호길 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.5

The aim of this study was to investigate the susceptibility of mutans streptococci (S. mutans and S. sobrinus) and Streptococcus anginosus, for seven antibiotics, penicillin G, amoxicillin, ciprofloxacin, cefuroxime, erythromycin, bacitracin, and vancomycin. The minimum inhibitory concentration (MIC) of seven antibiotics against 3 species (type strains) of mutans streptococci and S. anginosus, 10 strains (wild type) of S. mutans, 7 strains (wild type) of S. sobrinus, and 11 strains (wild type) of S. anginosus, were measured by broth dilution method. All of the type strains of mutans streptococci and S. anginosus had the same susceptibility for penicillin G, amoxicillin, cefuroxime and bacitracin. Type strain of S. anginosus was sensitive in ciprofloxacin, but those of mutans streptococci were not. All of the clinical isolates of mutans streptococci and S. anginosus had the same susceptibility for the seven antibiotics. Our data reveal that mutans streptococci and S. anginosus have similar antibiotic-resistant character. In addition. these results may offer the basic data to verify the antibiotic-resistant mechanism of mutans streptococci and S. anginosus.

미생물의 식별법

국중기,임채광,김미광 朝鮮大學校 口腔生物學硏究所 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.1

The aim of this review is to introduce the methods for bacterial identification. It has been developed many bacterial identification methods; cell culture, biochemical test, DNA-DNA hybridization, DNA typing, antigenic typing, multilocus enzyme electrophoresis, 16S or 23S rRNA gene sequencing, polymerase chain reaction. These have several disadvantages as well as advantages. Therefore, for choosing the proper method, we must understand the mechanism and process of the method for bacterial identification.

인유두종바이러스 type16과 화학발암물질로 형질전환된 사람 구강각화세포에서 viral promotoer p97의 활성 변화 및 조절에 관한 연구

국중기,이진,우경미,민병무 서울大學校 齒科大學 1996 齒大 論文集 Vol.20 No.2

DNA probe Fu9 을 이용한 Fusobacterium nucleatum strains의 동정

국중기,한진주,임선아,백현민,김동기 朝鮮大學校 口腔生物學硏究所 2001 口腔生物學硏究 Vol.25 No.2

The purpose of this study was to isolate DNA probe which could identify Fusobacterium nucleatum strains in Southern blot analysis. This study procedure includes ⑴ shole-genomic DNA extraction of Fusobacterium nucleatum (F . nucleatum) ATCC 25586, F. nucleatum ATCC 237626, F. nucleatum ATTC 10953, F. nucleatum ATTC 49256, F. nucleatum ATCC 51190 (2) construction of the genomic DNA library. ⑶ screening of restriction fragment of genomic DNA, ⑷ identification of species-specific DNA probe by Southern blot hyridization. About 150 restriction fragements of several F. nucleatum genomic DNA digested with the Hind Ⅲ were obtained. One of them, the probe Fu9, restriction fragements of F. nucleatum Fusobacterium nucleatum ATCC 25586, was shown to be hybridized with all of the F. nucleatum strains used in this study but the length of the bands are different among the strains. From that result, the probe Fu9 may be used in identification of F. nucleatum stains.

Campylobacter rectus ATCC 33238에 대한 종 : 특이 DNA 프로브의 클로닝

국중기,정학균,성진효,손재범,김병옥,김동기 대한구강보건학회 2002 大韓口腔保健學會誌 Vol.26 No.4

The purpose of this study is to develope strain-specific DNA probes and polymerase chain reaction (PCR) primers for detection and identification of Camphyrobactor rectus (C, rectus) ATCC 33238. This study procedure included (1) whole-genomic DNA extraction of C. rectus ATCC 33238, (2) construction of the genomic DNA library, (3) screening of restriction fragment of genomic DNA by reverse dot hybridization, (4) isolation of strain-specific DNA probes by Southern blot hybridization. Thirty-five restriction fragments of C, rectus ATCC 33238 genomic DNA digested with the HindⅢ were obtained. Southern blot analysis data showed that two of them, Cr44-3 (832 bp) and Cr44-4 (669 bp) were C. rectus ATCC 33238 specific DNA probes. Our data indicate that these DNA probes may be useful in detection and identification of the C rectus ATCC 33238.

용이한 Subcloning 을 위한 pCAT�3-Basic vector의 변형

국중기,한진주,김명수,박주철,김흥중 朝鮮大學校 口腔生物學硏究所 2001 Oral Biology Research (Oral Biol Res) Vol.25 No.2

To increase convenience and facilitate subcloning in construction of chloramphenicol acetyl transferase (CAT) plasmids for transient expression assay, modification of the pCAT^(R)3-Basic vector were performed. The procedures of this study were as follows : ⑴ The pCAT^(R)3-Basic vector was digested with bamH I and Sal I and then the 5'-end overhanging were filled with Klwnow Fragment and dNTPs. ⑵ The resulting blunt-end DNA fragment was self-ligated using T4 DNA ligase. ⑶ The ligation mixture was transformed into E. Coli DH5α. ⑷ The modified pCAT^(R)3-Basic vector was named pCAT^(R)3△B/S-Basic vector. ⑸ The pCAT^(R)3△B/S-Basic vector was digested with Xba I and then the 5'-end overhanging were filled with Klenow Fragment and dNTPs. ⑹ The result modified pCAT^(R)3△B/S-Basic vector was named pCAT^(R)3b-Basic vector. ⑺ The multiple cloning site (from Kpn I to Xho I sites) of pCR2.1-TOPO cloned into the pCAT^(R)3b-Basic vector. The resulting plasmid was named pCAT^(R)3b-CR-Basic vector. As the result, the new vector. pCAT^(R)3b-CR-Basic vector. As the result, the new vector. pCAT^(R)3b-CR-Basic vector has more five single cloning stites-BamH I, Spe I, BstX I, EcoR V, and EcoR I- than the pCAT^(R)3-Basic vector. This data indicate that the pCAT^(R)3b-CR-Basic vector can be useful for constructioin of CAT plasmids in conventience and facility.

Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046

국중기 KOREAN ACADAMY OF ORAL BIOLOGY 2011 International Journal of Oral Biology Vol.36 No.2

The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strainspecificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.