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플라스미드 DNA로 대장균 형질전환시 회복단계의 필요여부에 관한 연구
김미광 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2
We previously introduced the direct transformation method, direct plating of transformation mixture onto LB agar plate after immediately mixing of competent cells and pUC19 plasmid DNA. In that report, the difference of transformation efficiency according to the recovery time of transformed E. coli with pUC19 was not determined. In this study, to completely establish the direct transformation method, transformation efficiency according to the recovery time of transformed E. coii was determined. Our data reveal that the transformation efficiency was increased the recovery time after heat shock. However, this result come from the mitosis of transformed E. coli with pUC19. Our data indicate recovery step may be omitted in transformation experiment.
한국인의 치주질환 환자의 치은연하치면세균막에서 Prevotella intermedia와 Prevotella nigrescens의 검출율 비교
김미광 ( Mi Kwang Kim ),성진효 ( Jin Hyo Seong ),김동기 ( Dong Kie Kim ),손영남 ( Young Nam Son ),이장재 ( Jang Jae Lee ),김흥중 ( Heung Joong Kim ),박주철 ( Joo Cheol Park ),장현선 ( Hyun Seon Jang ),김병옥 ( Byung Ock Kim ),신 대한구강보건학회 2004 大韓口腔保健學會誌 Vol.28 No.2
DNA probe Pn25를 이용한 Prevotella intermedia 및 Prevotella nigrescens의 동정
김화숙 ( Hwa Sook Kim ) , 김미광 ( Mi Kwang Kim ) , 유소영 ( So Young Yoo ) , 최민호 ( Min Ho Choi ) , 임상수 ( Sang Soo Lim ) , 박헌동 ( Heon Dong Park ) , 송수근 ( Soo Keun Song ) , 신환선 ( Hwan Seon Shin ) , 박슬희 ( Seul H 조선대학교 구강생물학연구소 2003 Oral Biology Research (Oral Biol Res) Vol.27 No.2
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용이한 Subcloning을 위한 pCAT(R)3-Basic vector의 변형 2
유소영 ( So Youog Yoo ) , 김미광 ( Mi Kwang Kim ) , 김화숙 ( Hwa Sook Kim ) , 최민호 ( Min Ho Choi ) , 임상수 ( Sang Soo Lim ) , 박헌동 ( Heon Dong Park ) , 송수근 ( Soo Keun Song ) , 신환선 ( Hwan Seon Shin ) , 박슬희 ( Seul H 조선대학교 구강생물학연구소 2003 Oral Biology Research (Oral Biol Res) Vol.27 No.2
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강미광,김명지,Kang, Mee-Kwang,Kim, Myung-Jee 영남수학회 2008 East Asian mathematical journal Vol.24 No.5
One of the education purpose of the section "Figures" in the eighth grade is to develop students' deductive reasoning ability, which is basic and essential for living in a democratic society. However, most or middle school students feel much more difficulty or even frustration in the study of formal arguments for geometric situations than any other mathematical fields. It is owing to the big gap between inductive reasoning in elementary school education and deductive reasoning, which is not intuitive, in middle school education. Also, it is very burden for students to describe geometric statements exactly by using various appropriate symbols. Moreover, Usage of the same symbols for angle and angle measurement or segments and segments measurement makes students more confused. Since geometric relations is mainly determined by the measurements of geometric objects, students should be able to interpret the geometric properties to the algebraic properties, and vice verse. In this paper, we first compare and contrast inductive and deductive reasoning approaches to justify geometric facts and relations in school curricula. Convincing arguments are based on experiment and experience, then are developed from inductive reasoning to deductive proofs. We introduce teaching methods to help students's understanding for deductive reasoning in the textbook by using stepwise visualization materials. It is desirable that an effective proof instruction should be able to provide teaching methods and visual materials suitable for students' intellectual level and their own intuition.
감염근관에서 분리 배양한 세균의 수종 항생제에 대한 감수성 조사
임상수,김미광,민정범,김민정,박순낭,황호길,국중기,Lim, Sang-Soo,Kim, Mi-Kwang,Min, Jeong-Beom,Kim, Min-Jung,Park, Soon-Nang,Hwang, Ho-Keel,Kook, Joong-Ki 한국미생물학회 2006 미생물학회지 Vol.42 No.3
본 연구는 치근관 감염병소에서 세균을 분리 및 동정하고, 8종의 항생제들에 대한 분리균주들의 감수성을 조사하기 위하여 실시하였다. 세균에 감염된 27개 치아의 괴사된 치근부 치부소직을 바비드 브로치나 페이퍼 포인트로 무균적으로 채취하였다. 치수가 채취된 부위의 바비드 브로치와 페이퍼 포인트를 500 ul의 $1{\times}PBS$ 용액에 담아 잘 혼합하고, 이를 5% 양혈이 포함된 BHI 한천배지(혈액한천배지)에 도말하여 $37^{\circ}C$ 혐기성 배양기에서 2-5일 동안 배양하였다. 혈액한천배지에서 자라난 세균은 16S rRNA 유전자(rDNA) 염기서열결정법을 이용하여 종수준으로 동정하였다. 이들 균주들의 8종 항상제에 대한 감수성은 최소성장억제농도 측정법으로 조사하였다. 본 연구결과, 101개의 세균 집락이 생겼고, Streptococcus spp. (29.7%)와 Actinomyces spp. (21.8%)가 가장 많이 검출되었다. 이들 균주들 중 9균주는 실험 도중 소실되거나 액체배지에 자라지 않아서 항생제 감수성 심험에서는 제외되었다. 각 항생제들에 대한 감수성을 조사한 결과, 분리된 균주들 중 80(87.0%) 균주가 클린다마이신에 감수성을 보였으며, 세프록심 아세틸과 테트라사이클린에 69(75.0%)가, 오그멘틴에 66(71.7%) 균주가, 페니실린 G에 63(68.5%) 균주가, 에리트로마이신에 61(66.3%) 균주가, 아목시실린에 41(44.6%) 균주가 감수성을 보였다. 그러나 시플록사신에는 29(31.5%) 균주만이 감수성을 보였다. 이러한 8종 항생제에 대한 균주들의 감수성 양상은 세균 종의 종류보다는 분리된 숙주에 따라 차이가 있었다. 이러한 결과는 치근관 감염질환의 치료에 항생제가 필요할 경우 항생제 감수성검사를 병행하는 것이 효과적임을 시사한다. The aim of this study was to identify the bacteria isolated from endodontic lesions by cell culture and to determine the antimicrobial susceptibility of them against 8 antibiotics. The necrotic pulpal tissues were collected from 27 infected root canals, which were diagnosed as endodontic infection. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing $500{\mu}l\;of\;1{\times}PBS$. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a $37^{\circ}C$ anaerobic chamber for 2 to 5 days. The bacteria grown on the agar plates were identified by comparison of 16S rRNA gene (rDNA) sequencing method at the species level. To test the sensitivity of the bacteria isolated from the infected root canals against 8 antibiotics, minimum inhibitory concentrations (MIC) were determined using broth dilution assay. The data showed that 101 bacterial strains were isolated and were identified. Streptococcus spp. (29.7%) and Actinomyces spp. (21.8%) were predominantly isolated. The 9 strains were excluded in antimicrobial susceptibility test because they were lost during the experiment or were not grown in broth culture. The percentage of bacteria susceptible for each antibiotic in this study was clindamycin, 87.0% (80 of 92); tetracycline, 75.0% (69 of 92); cefuroxime axetil, 75.0% (69 of 92); amoxicillin + clavulanic acid (5:1), 71.7% (66 of 92); penicillin G, 66.3% (61 of 92); erythromycin, 66.3% (61 of 92); amoxicillin, 44.6% (41 of 92); and ciprofloxacin, 31.5% (29 of 92). The susceptibility pattern of 8 antibiotics was dependent on the host of the bacteria strains rather than the kinds of bacterial species. These results indicate that antibiotic susceptibility test should be performed when antibiotics are needed for the treatment of infected root canals.