한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

비만증 환자에서 한약제에 의한 체중 감소효과

Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.

새로운 Acetylcholinesterase inhibitors의 합성

최순규,김형민,조승환,최학기,박유미,이용균,정대일,김인식,한정태 동아대학교 부설 기초과학연구소 2004 基礎科學硏究論文集 Vol.21 No.1

노인성 치매의 일종인 alzheimer's disease의 효과적인 억제제를 합성하기 위해 우리는 분자 모델링에 의한 가장 적합한 물질인 m-[(N,N,N-trimethylammonio)phenyl]boronic acid를 합성하였다. 출발물질인 3-aminophenylboronic acid monohydrate의 경우 boronic acid의 작용기인 hydroxyl group을 protecting시킴으로써 반응의 안정성을 기하였다. Quarternary ammonium salt는 과량의 methyliodide와 염기 촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시켜 상당히 높은 수율을 얻을 수 있었다. 또한 과량의 methyliodide와 염기촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시킨 결과 boronic acid의 protection없이도 안정하게 반응이 진행되어짐을 확인할 수 있었다. In order to syntheisize a effective inhibotor for alzheimer's disease, we synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 which is designed by molecular modeling form. We protected the hydroxyl group of 3-aminophenylboronic acid monohydrate 1 with ethlyne glycol to remove the reactivity if hydroxyl group. To synthesize m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4, we reacted 3-aminophenylboronic acid monohydrate 1 with ezcess methyl iodide and potassium hydrogen carbonate as a base-catalyst in methanol. but On executed reaction without protection on hydroxyl group, we found out the result that the hydroxyl group of boronic acid group at 3-aminophenylboronic acid monohydrate 1 didn't react with excess methyliodide. Synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 is in progress about biological tests as a plausible acetylcholinesterase inhibitor.

고출력 다이오드 레이저를 이용한 SKD11 표면 열처리 특성

최성대,정선환,양승철,김잠규 한국공작기계학회 2007 한국공작기계학회 추계학술대회논문집 Vol.2007 No.-

An experimental investigation with high power diode laser system was carried out to study the effect of surface heat treatment on the SKD11. The surface heat treatment characteristics of the laser beam were evaluated using hardness tests, optical microscopy, X-ray diffraction and energy dispersive X-ray spectroscopy(EDS). Results indicated that the Hardness were changed by beam size, focal length, feed rates. The micro grain structure was observed retained austenite grain at hardened zone and globular cementite grain at fine grain zone and base metal zone by optical microscopy. The intensive X-ray diffraction patterns of (110)-(200)-(211) was detected on the surface and the hardened surface intensity level was bigger than the unhardened surface. The intensive X-ray diffraction patterns of (110)-(200)-(211) was detected hardened surface and the hardened surface distributed plenty of carbon density than metal zone.

춘천 중도 선사유적 발굴조사 연구 : 고인돌을 중심으로;Concentrated on the Dolmen Site

최복규,원영환,손승철,신종원 江原大學校 江原文化硏究所 1983 강원문화연구 Vol.3 No.-

The Joong-do prehistoric site is located in the Lake of ?am dam of Chuncheon City, the capital of Kangweon province situated on the confluence of the North Han River and the Soyang River. This island site, it is washed away by the differance between the rise and fall of the lake water level, every year since 1967. The appoximate locations of Joong-do site is at 37℃ 52′;northern latitude, and 127℃ 51′ eastern longitude. The elevation of the present cultivation area is appoximately 80 meter above sea-level. This prehistoric sites, possibly ranges from Neolithic and Bronze Age to early lron Age, was brought to light in the consecutive excavations and surveys from 1981 to 1983 by the Institute of Kangweon Community Culture and the Museum of Kangweon National University. Some dolmens and ancient tombs (stone mounded), various stone tools, potteries and other relics of Neolithic, Bronze Age and early Iron Age were discovered by survey work of our excavation team from the surface and disturbed areas in the island site since 1981. During the excavation work from November 2 to December 1 in 1983, we were excavated a dolmen at center in the inland. The dolmen have four pieces of stone slab were used to make an oblong chamber covered by a large flat top stone of triangular form(size;270×196×40㎝, weight;3 ton). Both the top stone and the stone chamber surrounded by stone mounds for burial is buried under the surface. Three stone slabs of chamber coffin are required to support, but rest one inverse trapezoid stone slab of westward was not to support the top stone. The subsistance of unsupported stone slab suggested the existence of a stone chamber of which one side is open. We presumed the open side was used as a door of stone coffin. The bottom of the stone coffin under top stone was made by means of small pebble stones and large pebble stone slabs from the adjacent river beds. Size of stone slabs of coffin is at 191×94×13㎝;southern, 171×81×13㎝;northern, 80×72×9㎝;eastern. It seems, however, chronology of the dolmen are comparable to the later period of the plain coarse pottery culture(B.C.3~2C) in late Bronze Age.

다운 증후군 정신지체인의 시각적·언어적 자극에 대한 반응 시간

최승오,김동환 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

Previous research has indicated that individuals with Down Syndrome(DS) have difficulties processing auditory movement information relative to their peers with undifferentiated developmental disabilities. The present study was conduced to assess whether a model of atypical cerebral specialization could explain these findings. 13 adults with Down syndrome (8 men and 6 women), 14 adults with undifferentiated developmental disabilities (7 men and 7 women), and 14 adults without disabilities (8 men and 6 women) performed rapid aiming movements to targets under three conditions: a visual cue at the target location a visual cue remote from the target location or a verbal cue. Results revealed that, while the reaction times did not differ between the two groups with disabilities across conditions, the participants with DS, unlike their peers, had significantly longer movement times in the verbal than in two visual conditions. These results are consistent with the model of biological dissociation.

人權條約의 效用性 : 國際人權規約을 중심으로

崔昇煥 國際人權法學會 1998 國際人權法 Vol.2 No.-

익산시 주변 하천 담수조류군집의 다양성에 대하여

최환석,김미연,김백호,최민규,박승택 원광대학교 환경과학연구소 2000 環境科學硏究誌 Vol.9 No.1

차체 구조의 빔모델링에 관한 연구

최종근,이승훈,최석환 도립 강원전문대학 1998 道立 江原專門大學 論文集 Vol.1 No.1

This paper has been studied to analyze an automotive body structure by means of a simplified beam model rather than a detailed shell model. It has been done to enhance analysis performance to facilitate design changes. Basically automotive body consists of box channel structures and the strain energy for deformation is concentrated in the vicinity of joint area. Therefore analysis errors from beam modeling are due to the difference in strain energy of joint area between beam and shell modeling. Technique using modified section properties has been introduced, and some important nondimensional factors have been also introduced. We have found out the ratio of thickness and section length, t/H is the main factor to decide whether we have to use this new technique in beam modeling of box channel structures with joint. This technique shows the same results from shell modeling within 2 % error, both in static and normal mode analysis for an isolated model.

체외 충격파 쇄석기에 의해 발생된 음향 흐름 현상 연구

최민주,이승환,홍경진,김호찬,박재우,이헌주 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

Acoustic streaming produced by an extracorporeal shock wave lithotripter was visualized for the first time in the present study. It was observed that the streaming velocity increased with the shock wave amplitude and was reaching up to 3 cm/s at the highest output setting of the lithotripter.