H. pylori 검출을 위한 CLO 검사, Geimsa 염색 및 면역조직화학 염색의 비교 분석

유숙 ( Suk Yu ),백운철 ( Oun Chul Back ),김종열 ( Jong Yull Kim ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

In order to detect a rational method for reducing the ratios of the false positive and the false negative appearing from the CLO test and giemsa staining used for the examination of H. pylori, we have compared with the results of CLO test and giemsa staining of which the false positive and the false negative are shown through this study. We have divided the groups as following four groups: A, B, C, and D group. And as for the respective group we have executed the immunohistochemical staining reported as the highest in terms of sensitiveness and particularity and have got to the following results through the reciprocal comparisons of H. pylori detection results. 1. A Group; CLO test positive and giemsa staining positive As a result, immunohistochemical staining showed a higher degree rather than giemsa staining in terms of sensitiveness. 2. B Group; CLO test negative and giemsa staining positive As a result, false positive of giemsa staining against the results of the negative of immunohistochemical staining was elaborated as stained in the mucus and the similar bacteria. And the false negative of CLO test that does not coincide with the results of immunohistochemical staining was elaborated as inadequacy of the option of the organic extraction. In such a way, immunohistochemical staining showed a higher degree rather than CLO test in terms of the sensitiveness and particularity. 3. C Group; CLO test positive and Giemsa staining negative As a result, false negative was judged as stained bacteria as the number of H. pylori is not so many or colored in blue color in the stain background in the giemsa staining. And the false positive of CLO test was elaborated as polluted from bacteria in the inside of the oral cavity at the time of organic extraction. 4. D Group; CLO test negative and giemsa staining negative Immunohistochemical staining showed negative in all for cases, and coincided with the results of CLO test and giemsa staining. In conclusion, immunohistochemical staining was made in only H. pylori through antigen and antibody. CLO test and giemsa staining showed positive and negative in gastric mucosa. Therefore, as for the detection body of which the results of CLO test and giemsa staining does not coincide like immunohistochemical staining, to let reconfirm through the examination method of high sensitiveness and high characteristics would be of help for an exact detection of H. pylori and an earlier treatment.

위점막에서 조직화학적 및 면역조직화학적 염색을 이용한 Helocibaxter pylori 의 검출

백종철(Jong Cheul Baek),강헌석(Heon Seok Kang),이숭(Soong Lee),명재일(Jae Il Myung),김완(Wan Kim),박창수(Chang Soo Park) 대한내과학회 1997 대한내과학회지 Vol.53 No.5

N/A logic test (ELISA, BIO-RAD GAP method), histochemical stain (Warthin-Starry silver stain), immunohistochemical stain (Avidin biotin conjugate method, ABC method) were performed to identify the presence of H. pylori infection. H. pylori infection was defined that 2 of 3 studies were positive. Results: Positive result to H. pylori infection by serologic test, Warthin-Starry silver stain and immunohistochemical stain were 82% (28/34 cases), 65% (22/34 cases), 68% (23/34 cases). Sensitivity and specificity were 68%, 20% in serologic test, 95%, 83% in Warthin-Starry silver stain, 100%, 100% in immunohistochemical stain. Correlation among these tests was best between Warthin-Starry silver stain and immunohistochemical stain. Conclusion: By the above results, we can conclude that histochemical stain and immunohistochemical stain are the most accurate diagnostic method to identify the infectivity of R. pylori.

결핵균 발견에 관한 면역조직화학적 염색과 Ziehl-Neelsen 염색의 비교

조미연,정순희,양우익 대한병리학회 1993 Journal of Pathology and Translational Medicine Vol.27 No.3

Sporothrix schenckii 선별검사를 위한 항BCG 면역조직화학 염색법의 적용 : PAS 염색법과의 비교 Comparison with PAS Stain

박진영,이승철,원영호 대한의진균학회 1999 대한의진균학회지 Vol.4 No.1

Background: Sporotrichosis is frequently difficult to diagnose because its morphologic appearance may closely resemble mycobacterial infection or noninfectious inflammatory reaction. S. schenckii may be difficult to identify in histological sections stained with histochemical stainig such as haematoxylin and eosin, periodic-acid-Shiff (PAS) and Gomori's-methenamine-silver (GMS). Objective: The purpose of this study was to determine whether immunohistochemical staining with a polyclonal anti-Mycobacterium bovis (BCG) antibody, which is known for its interspecies cross-reactivity between microorganisms, is the suitable screening method to detect S. schenckii of sporotrichosis in skin tissues. Methods: Thirty sporotrichosis samples of formalin-fixed, paraffin-embedded skin tissues were stained with anti-BCG immunohistochemical stain (anti-BCG stain) and PAS stain. Results: Thirteen cases (43%) of 30 were positive with PAS stain, and 19 cases (63%) were positive with anti-BCG stain. In 24 cases which were positive in fungal culture, 11 were positive in both stains, 1 was positive in PAS stain only, 6 cases were positive in anti-BCG stain on and 6 were negative in both stains. In 6 cases which were negative in fungal culture, 1 was positive in both stains, 1 was positive in anti-BCG stain, and 4 were negative in both stains. Conclusion: Because of its cross-reactivity with fungi as well as its high sensitivity a minimal background staining, the anti-BCG stain can be the useful screening method of detection of S. schenckii in paraffin-embedded specimens of sporotrichosis.

난황낭종으로 변형된 난소의 미분화세포종 : 면역조직화학적 연구

고재환(Jae Whoan Koh),강윤경(Yoon Kyung Kang),김용봉(Yong Bong Kim),이응수(Eung Soo Lee),박성관(Sung Kwan Park) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.1

N/A Objective: The purpose of this article is to report mixed germ cell tumor, which revealed changes compatible with early transformation of dysgerminoma to endodermal sinus tumor(EST) through histogenetic considerations and immunohistochemical stains. Methods: Ovarian germ cell tumors were reviewed from files of Dept. Ob/Gyn. Seoul Paik Hospital fiom 1992.1 to 1996.12. Total of 5 cases include 4 pure dysgerminoma and 1 mixed germ cell tumars. All tissues were fixed in 10% neutral buffered formalin and embedded in paraffin and reviewed by two pathologists with immunohistochemical staining for cytokeratin, vimentin, AFP, PCNA, p53 & bc1-2. Results: Grossly, the areas of transformation were located at the middle of the mixed tumor. The outer layer of the tumor mass was filled with typical pure dysgerminoma. They were characterised as the presence of microcysts and small glandular structures in hematoxylin-eosin(H-E) stains with positive stain for vimentin, except the tissue of the EST. The cells in the intermediate layer were characterised as the mixed form of dysgerminomatous and EST structures in H-E stains. AFP in the dysgerminomatous cells in intermediate layer and EST were stained, but not in outer layer. Conclusion: Dysgerminoma may possess the ability to transform to EST. There might be intermediate stage between dysgerminoma and EST, and Immunohistochemical staining for AFP, cytokeratin, vimentin, PCNA also can be used for prognosis of germ cell tumor.

결장 및 직장암 환자에서 혈청과 면역조직화학 염색에서 CA19-9의 의의

김민수(Min Soo Kim),주호민(Ho Min Joo),홍성우(Seong Wo Hong),강윤경(Yun Kyung Kang),천정우(Jung Woo Chun),장여구(Yeo Goo Chang),백인욱(In Wook Paik),이혁상(Hyucksang Lee) 대한외과학회 2008 Annals of Surgical Treatment and Research Vol.75 No.5

Purpose: There has been much debate about the significance of the CA19-9 level for predicting the prognosis of colorectal cancer patients. This study aimed to evaluate the prognostic value of the preoperative serum CA19-9 level and the CA19-9 expression in the tumor tissues of colorectal cancer patients Methods: One hundred patients with colorectal cancer and who had been treated by resection were studied. We assessed the correlations of the preoperative serum CA19-9 level and the status of the CA19-9 immunohistochemical staining with the clinicopathologic features, including the prognosis of the patients. Results: The preoperative serum CA19-9 level had significant correlation with the status of CA19-9 immunohistochemical staining. The presence of distant metastasis was significantly correlated with an elevated level of serum CA19-9. The depth of tumor, the presence of lymph node metastasis, the TNM stage and tumor cell differentiation were significantly correlated with the status of the CA19-9 immunohistochemical staining. In addition, the gross morphology, depth of tumor, the presence of lymph node metastasis, the TNM stage, the status of the CA19-9 immunohistochemical staining and the serum CEA level were correlated with survival on univariate analysis. However, multivariate analysis did not validate the status of CA19-9 immunohistochemical staining as a significantly independent predictor of the prognosis. Conclusion: The CA19-9 expression was frequently observed in advanced stage tumor tissue, yet its expression in tumor tissue or the preoperative CA19-9 serum level did not show independent prognostic value for colorectal cancer patients.

면역조직화학적 방법을 이용한 흰쥐 골격근 근섬유형의 비교연구

이종은,이원택,박승화,박경아 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

Programmed Cell Death in Placenta

Kim, Yong-Beom,Kim, Jeong-Kyu,Hong, Seung-Hwa,Won, Choong-Hee,Lee, Seok-Jae,Hong, Chang-Soo,Ji, Ill-Woon,Jeong, Eun-Hwan,Kim, Hak-Soon 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: 인간과 토끼의 태반에서 나타나는 세포고사(programmed cell death)의 양상을 알아보고자 하였다. 대상 및 방법 : 임신 17주에서 41주의 21명의 산모에서 얻어진 조직학적인 병변이 없는 태반과 임신 10일, 19일, 20일 그리고 27일의 토끼에서 얻어진 태반을 대상으로 연구하였다. 인간의 태반에서는 bcl-2의 발현양상을 면역조직화학염색과 immunoblotting analysis를 이용하여 검사하였고 세포고사가 나타나는 양상을 알아보기 위하여 TUNEL 염색과 agarose gelelectrophoresis of genomic DNA를 시행하였다. 또한 토끼의 태반에서 나타나는 세포고사의 양상을 알아보기 위하여 H&I 염색과 TUNEL 염색을 시행하였다. 결과: 임신 32주 이전의 태반에서는 모두에서 bcl-2의 발현이 양성이었다. 세포고사는 태반의 모든 조직에서 발견되었고 특히 탈락막 세포에서 많이 발견되었으며 세포고사 지수는 평균 4.57%이었다. 그러나 임신 주수에 따른 세포고사 지수의 변화는 통계학적으로 유의하지 않았다. 토끼의 태반에서도 세포고사는 H&I 염색과 TUNEL 염색으로 쉽게 관찰할 수 있었고 인간에서와 마찬가지로 탈락막에서 가장 많이 발견되었다. 결론: 태반에서 나타나는 세포고사는 인간과 토끼에서 모두 정상적으로 나타나는 소견이었다. 태반의 기저층에 해당하는 탈락막에서 세포고사가 가장 많이 나타났고 임신이 진행될수록 세포고사가 많이 나타났다. Purpose : We investigated the patterns of the programmed cell death (PCD) throughout the gestation in human and rabbit placental tissues. Materials and Methods: A total of 21 human placentas of gestational ages ranging from 17 weeks to 41 weeks which were devoid of histopathologic abnormalities and four rabbit Placentas of gestational ages of day 10, 19, 20 and 27 were obtained We have examined the patterns of bcl-2 expression in human placentas using immunohistochemical staining and immunoblotting analysis. We have used TUNEL staining and agarose gel electrophoresis of genomic DNA to ensure the patterns of PCD in human placentas. We also analyzed the pattern of PCD in rabbit placentas using hematoxylin and eosin (H&E) staining and TUNEL staining. Results : All of the placentas before the gestational age of 32 weeks were definitely positive for bcl-2 expression. PCD was observed in all cellular compartments of placenta and intermediate trophoblasts and decidual cells showed higher Prevalence of PCD. Apoptotic index was ranged from 3.30% to 5.67% (mean 4.57%). However, significant difference in the frequency of PCD according to the gestational age could not be demonstrated. In rabbit placenta, patterns of PCD were easily identified with TUNEL staining and hematoxylin and eosin staining and placental floor and decidua basalis were, as was suggested from human placenta, the major sites where PCD took place. Conclusion: We have clearly demonstrated that PCD is a normal finding in the placenta and decidua of both human and rabbit. The placental floor and decidua, the site of separation of fetomaternal interphase during the parturition, are the major compartment undergoing PCD. And also we have identified that PCD occurs more frequently with advancing gestation.

Immunohistochemical Study of Myogenic Sarcomas

Lee, Kyo Young,Kang, Chang Suck,Kim, Won Il,Shim, Sang In,Kim, Sun Moo CATHOLIC MEDICAL CENTER 1988 Bulletin of the Clinical Research Institute Vol.16/17 No.1

Using immunohistochemical staining with monoclonal antibodies to muscle cell components, histogenetical grouping and differential diagnosis of myogenic sarcoma could be possible. However immunohistochemical staining properties of myogenic sarcomas are not well defined in the formalin-fixed, paraffin-embedded tissue. We attempted to differentiate leiomyosarcoma from rhabdomyosarcoma. Twenty-nine formlin fixed myogenic sarcomas were investigated by means of immunohistochemical technique with the use of monoclonal antibodies panel against vimentin, desmin and myoglobin. In addition, the degree of differentiation of these sarcomas was investigated in connection with the immunoreactivity and intensity of immunohistochemical stains. The results were as follows; 1. Vimentin was detected in nine (60%) out of fifteen cases of leiomyosarcoma and in seven (50%) out of fourteen cases of rhabdomyosarcoma. 2. Desmin was detected in seven (47%) out of fifteen cases of leiomyosarcoma and in eight (57%) out of fourteen cases of rhabdomyosarcoma. 3. Myoglobin was detected in eleven (79%) out of fourteen cases of rhabdomyosarcoma. In all leiornyosarcomas, myoglobin was not detected. 4. The immunoreactivity and intensity of these sarcomas with anti-desmin and anti-myoglobin were related to the degree of tumor cell differentiation, but vimentin was more frequently detected in the poorly differentiated tumor cells. The above results suggest that demonstration of vimentin, desmin and myoglobin in the formalin-fixed, parnffin-embedded tissue may be a helpful adjunct tool in the diagiosis of myogenic sarcoma.

소뇌 모세혈관성 혈관모세포종의 간질 세포 성상에 관한 연구 - 면역조직화학적 분석 -

홍순원,김태승,한지영 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.5