위암에서 종양관련 단백질들과 COX-2 발현의 상관성

유숙 ( Suk Yu ),노정환 ( Jeong Hwan Noh ),김종열 ( Jong Yull Kim ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3

It has been reported the higher incidence rate of gastric cancer in our country. Helicobacter pylori (H. pylori), that exhibited a higher infection rate among Korean people, has been known as a cofactor to cause cancer. Therefore, the aim of this study was to identify correlations among overexpressions of COX-2 (Cyclooxygenase-2) gene, p53 mutation and cell proliferation index related to H. pylori. Taking 28 cases of gastric cancer with H. pylori detection, immunohistochemical staining for COX-2, p53 and Ki-67 were performed. In the H. pylori positive group, the well differentiated type and diffuse type of gastric cancer were distributed in larger area and the expression rate of COX-2 was revealed high. The H. pylori negative group showed higher p53 expression than that of the positive group. However, the statistical correlation between H. pylori and histopathological factors was not observed. The significantly higher expression of COX-2 had were observed in both well differentiated type and the intestinal type of gastric cancer. Although there were no statistical significances, this showed a higher inclination of manifest in the early gastric cancer. p53 exhibited a higher tendency of expression in the well differentiated, moderately differentiated and the intestinal type of gastric cancers including the early gastric cancer. Ki-67 was expressed in a significantly higher fashion along with the increase of age. In addition, it was significantly expressed in well differentiated type and intestinal type of gastric cancer. Therefore, these results suggest that H. pylori, COX-2, p53, and Ki-67 influences on the new occurrence of gastric cancer and its development procedures. In the future, the more researches would be required to focus on a larger category relative to gene expressions in gastric cancer.

H. pylori 검출을 위한 CLO 검사, Geimsa 염색 및 면역조직화학 염색의 비교 분석

유숙 ( Suk Yu ),백운철 ( Oun Chul Back ),김종열 ( Jong Yull Kim ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

In order to detect a rational method for reducing the ratios of the false positive and the false negative appearing from the CLO test and giemsa staining used for the examination of H. pylori, we have compared with the results of CLO test and giemsa staining of which the false positive and the false negative are shown through this study. We have divided the groups as following four groups: A, B, C, and D group. And as for the respective group we have executed the immunohistochemical staining reported as the highest in terms of sensitiveness and particularity and have got to the following results through the reciprocal comparisons of H. pylori detection results. 1. A Group; CLO test positive and giemsa staining positive As a result, immunohistochemical staining showed a higher degree rather than giemsa staining in terms of sensitiveness. 2. B Group; CLO test negative and giemsa staining positive As a result, false positive of giemsa staining against the results of the negative of immunohistochemical staining was elaborated as stained in the mucus and the similar bacteria. And the false negative of CLO test that does not coincide with the results of immunohistochemical staining was elaborated as inadequacy of the option of the organic extraction. In such a way, immunohistochemical staining showed a higher degree rather than CLO test in terms of the sensitiveness and particularity. 3. C Group; CLO test positive and Giemsa staining negative As a result, false negative was judged as stained bacteria as the number of H. pylori is not so many or colored in blue color in the stain background in the giemsa staining. And the false positive of CLO test was elaborated as polluted from bacteria in the inside of the oral cavity at the time of organic extraction. 4. D Group; CLO test negative and giemsa staining negative Immunohistochemical staining showed negative in all for cases, and coincided with the results of CLO test and giemsa staining. In conclusion, immunohistochemical staining was made in only H. pylori through antigen and antibody. CLO test and giemsa staining showed positive and negative in gastric mucosa. Therefore, as for the detection body of which the results of CLO test and giemsa staining does not coincide like immunohistochemical staining, to let reconfirm through the examination method of high sensitiveness and high characteristics would be of help for an exact detection of H. pylori and an earlier treatment.

세포학적인 검사를 통해 자궁경부암의 조기 발견과 합리적인 병원내 산부인과의 운영 체계에 관한 고찰

김태전,배형준,김종열 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

In this study, the authers have investigated a true appreciation on the importance and the actual conditions of utilization of cytologic examination in gynecologic services with 3 step, that is, in 1 step the importance of cytologic examination in gynecologic services has been investigated from the results of 4,024 female of cytologic examination, in 2 step the actual conditions of utilization of cytologic examination have been investigated from 60 medical institutes through questionaire, and in 3 step the more detailed subjects of cytologic examination in gynecologic services have been investigated from 3 medical institutes selected randomly, such an investigations were done to make the programs of improvement in order to promote the activation of gynecologic medical services that shall be able to decrease occurrence of uterine cervical cancer, which is still the most prevalent cancer in korean female, and to make those on effective diagnosis and treatment activaton that shall be able to increase the income of medical institutes. The programs of improvement according to conclusions obtained from investigations like these were summarized as the following. 1. Program to activate positively cytologic examination It was well known that good correlation was noted between number of cytologic examination and those of histologic examination, those of gynecology procedures, and those of gynecologic surgeries. But it was a fact that a most of gynecology board certified physicians and physicians, and medical institutes have not utilized actively cytologic examination in gynecologic practice. Therefore all of married woman should be had politically cytologic examination at regular intervals in order to prevent and detect early uterine cervical cancer. 2. Program to establish exclusively responsible managed systems Managed systems that are exclusively responsible for cytologic examination should be established in order to have examination at regular intervals exactly(six months). 3. Program to utilize correctly the current medical insurance regulations about medical treatment charges The medical charges of cytologic exmination should be applied correctly to the current medical insurance regulations about medical treatment charges in order to increase the income of medical institutes. Because the medical charges of cytologic examination for the levels of medical check up and preventive medicin exception cancer or clinically suspicious cancer diagnosed by physicians is applicable to general medical treatment charges under the current medical insurance regulations. 4. Program to utilize reasonable diagnostic system in cytologic examination. Since certificated technologist screen first thing many microscopic slides for cytologic examination, lastly gynecologic board should make a final diagnosis on only abnormal slides. It is reasonable diagnostic system that can observe more microscopic slides, decrease misdiagnosis and high personnel expenses, and increase income in cytologic examination. Consequently it is necerssary to establish reasonable diagnostic system. 5. Program in order to promote the efficiency of the following examination and treatment system for patients to whom abnormal cell were detected. The following examination and treatment system that were corresponded with diagnosis, and automated and standardized system from receipt to gynecologic practice should be established to offer patients ease and trust, and also medical institutes the more income through the activation of gynecologic practice. Finally, the authers think that it is very important that cytologic examination should be included in routine gynecologic practice.

Music Board에 관한 硏究

金鍾烈 진주교육대학교 1969 論文集 Vol.1969 No.3

Music Board is a kind of the audio-visual aids and is not produced in Korea yet. But sooner or later it will be produced in the coming future in Korea. This music instrument can be made a sound of music when the head of the note which is written on the small blackboard is pressed with the baton connected with electirc wire. This music board is used as both the radio and electric gramophone, and the principle of it is that after vibrating the low frequency with the transistor 2SB56 and amplifying it, it can be heard. The characteristic of it is much effective to motivate the for learning. It gives not only audeo effect by the music note written on the blackboard but also visual-effect by pressing the note. Besides, the strengths of this instrument is that children cam easily learn the melody and play it at the sme time because there are note on the blackboard and the keyboard on the left of it as ahown the figure(1). The external form of the music board is like fig. (1), and the wiring of the part of the electirc channels is just like the fig. (2) and (3).