한국인 제2형 당뇨병 환자에 있어 지속적 피하 인슐린 주입법 치료시 Lispro insulin와 Regular insulin의 효과 비교

유재등,박봉안,류하근,류주성,김정수,김치훈,박선민,최수봉 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-

Objective: The Lispro insulin has faster onset of effects and shorter duration than the human regular insulin, an analog of human insulin. The differences in the pharmacodynamics between the two types of insulin in the continuous subcutaneous insulin infusion(CSII) with insulin pump for treating the Korean Type 2 DM patients were analyzed. The effects on the regulation of blood sugar, the amount of insulin injected, and the frequency of hypoglycemia were compared between the lispro insulin and the regular human insulin. Method and Material: One hundred and three Korean Type 2 DM patients were randomly divided into two groups, 49 Lispro insulin and 54 Regular insulin.(Table 1) Both groups were treated for 14 days. Also at a set time before meal, insulin was injected as bolus, using a portable insulin pump, in both groups, 15 minutes before for lispro insulin and 30 minutes before for human insulin The HbA1C, C-peptide, AC/PC, height and weight were measured for each patients at the time of admission. The peripheral blood sugar level and insulin injection profile were recorded 7 times per day for each patients. Results: After treating for 7 days and 14 days, the blood sugar level normalized in both groups. There weren't any significant differences in blood sugar levels in both groups at point of the study, and also there weren't any differences in the frequency of hypoglycemia. In order to maintain the normalized blood sugar level, the lispro insulin group had higher total daily insulin requirement than the regular insulin group after 14 days of treatment (0.90±0.35 vs 0.73±0.39u/kg/day, p<0.05) This difference is probably due to the difference in basement insulin requirements rather than prandial insulin requirements (0.32±0.1 vs 0.43± 0.09u/kg/day, p<0.001). Discussion: In the hospitalized Korean Type 2 DM patients with CSII treatment, continuous subcutaneous insulin infusion therapy using lispro insulin has convenience in usage compared to the regular insulin. However, there were not any differences in the blood sugar levels. Also lispro insulin requires more insulin in order to maintain normal blood sugar level, which is probably due to the increase in the basal requirement. Also there were not any difference in the frequency of hypoglycemia.

니페디핀 서방성 정제의 제제설계

최옥,김승수,박은석,지상철 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.2

Matrix tablets of nifedipine (NP) were prepared with Eudragit, diluent (lactose or Ca. phosphate) and Mg. stearate employing two different preparation methods (wet granulation and direct compression) to develop its sustainedrelease dosage forms. The effects of various formulation factors on the dissolution rate of the drug were investigated. Dissolution test was studied in pH 6.8 phosphate buffer containing 1 % sodium lauryl sulfate using the paddle method. Formulation factors were the type and content of Eudragit, the type of diluent and the tablet preparation method. The optimum formula of NP matrix tablet, which resulted in a similar dissolution profile to that from Adalat Oros used as a reference, was 30 mg NP, 10% Eudragit RS, 2% Mg. stearate and an adequate quantity of lactose to yield 500 mg weight using the wet granulation method.

Mo¨ssbauer Parameters of Fast Neutron-irradiated Nuclear Pressure Vessels at Room- and Liquid nitrogen-temperature

이용곤,김형상,홍치유,유연봉 동국대학교 자연과학연구원 2000 자연과학연구 논문집 Vol.5 No.-

The embrittlement of fast neutron-irradiated reactor pressure vessel (RPV) steels was investigated by X-ray diffraction patterns at room temperature and Mo¨ssbauer spectroscopy at room- and liquid nitrogen-temperature. Neutron fluence on the samples were 1012, 1013, 1014, 1015, 1016, 1017, 1018n/cm². The X-ray diffraction patterns at room temperature showed that the structure of neutron unirradiated sample was bcc type, where as but the neutron irradiated samples with the fluence higher than 1017 n/cm² were so severely damaged, that bcc type structure disappeared. The Mo¨ssbauer spectra of all samples showed superposition of two or more sextets. In this paper all Mo¨ssbauer spectra were fitted by three set of sextet. The isomer shift and quadrupole splitting values were found around zero. At liquid nitrogen temperature, magnetic hyperfine field increase rapidly to 480 ∼ 552 kOe at S1 sextet in the samples of 1017∼1018n/cm² neutron fluences. And room temperature, magnetic hyperfine field also increased rapidly to 480 ∼507 kOe at S1 sextet in the samples of 1017∼1018n/cm²neutron fluences. This rapid increase of magnetic hyperfine field was infered to be caused by the change of 56Fe, 55Mn into 57Fe by neutron irradiation.

기제와 피부투과촉진제가 아포모르핀의 피부투과에 미치는 영향

최영근,최옥,김건남,박은석,지상철 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

In order to evaluate the effects of vehicles and penetration enhancers on skin permeation of apomorphine, the skin permeation rates of apomorphine from vehicles of different composition were determined using Franz diffusion cells fitted with excised rat skins. Solubility of apomorphine in various solvents was investigated to select a vehicle suitable for the percutaneous absorption of apomorphine. The solvents used were propylene glycol (PG), Transcutol^??, Labrasol^??, Labrafac hydro WL^??, Labrafil WL 2609 BS^?? and isopropyl alcohol. Even though permeation rates of apomorphine from each vehicle were low (0.08-0.36㎍/㎠/hr), the combination of PG and Labrafac^?? increased it significantly. The permeation rates of apomophine from PG/Labrafac^?? mixtures increased as the volume fraction of PG in the mixture increased. The maximum permeation rate of 18㎍/㎠/hr was achieved at 30% of PG, which decreased with further increase of PG fraction. A series of fatty acids, alcohols and monoterpenes were employed as penetration enhancers. Incorporation of each enhancer in the PG/Labrafac^?? (30:70) mixture at the level of 10% improved the skin permeation significantly. The highest permeation rate, 117㎍/㎠/hr, was attained with myristic acid.

Mo¨ssbauer Effect of the Lithium- and Cesium- nitrosylpentacyanoferrates(Ⅱ)

김일구,류상훈,김형상,홍치유,심동훈,김순구 동국대학교 자연과학연구원 2000 자연과학연구 논문집 Vol.5 No.-

We observed the 57Fe Mo¨ssbauer spectra and Fourier transformation infrared spectra & X-ray fluorescence spectra of lithium nitrosylpentacyanoferrates(Ⅱ) (Li₂[Fe(CN)5NO]·4H₂O, Li₂NP) and cesium nitrosylpentacyanoferrates(Ⅱ) (Cs₂[Fe(CN)5NO]·H₂O, Cs₂NP) samples which comprise nitrosylpentacyanoferrates(Ⅱ) anions with a Fe atom center. The Mo¨ssbauer parameters, values of isomer shift and quadrupole splitting have been measured at room temperature and liquid nitrogen temperature. Also the Mo¨ssbauer Lamb factors of all samples have been measured at room temperature. We have drawn the experimental line width of the Mo¨ssbauer lines normalized to natural line width Γn versus effective thickness of 57Fe.

혈전성 혈소판감소성 자반증으로 발현된 전신홍반루푸스 1예

오명근,유미라,변유미,윤찬영,권세훈,박치영,김상현 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.3

Thrombotic thrombocytopenic purpura (TTP) is a life-threatening syndrome characterized by the classic pentad of clinical features that includes microangiopathic hemolytic anemia, thrombocytopenia, neurologic abnormalities, fever, and renal dysfunction. Early diagnosis and utilization of plasmapheresis can improve the survival rates of patients with TTP. TTP rarely may be seen in association with autoimmune disease such as systemic lupus erythematous (SLE). We report here a 42-year-old female who was presented with severe digital gangrenes, microangiopathic hemolytic anemia, thrombocytopenia, neurologic abnormalities, fever, and renal dysfunction. Her condition responded to combined therapy with high dose steroid, immunosuppressants, and plasmapheresis therapy.

Romo1 is associated with ROS production and cellular growth in human gliomas.

Yu, Mi Ok,Song, Na-Hyun,Park, Kyung-Jae,Park, Dong-Hyuk,Kim, Se-Hyuk,Chae, Yang-Seok,Chung, Yong-Gu,Chi, Sung-Gil,Kang, Shin-Hyuk M. Nijhoff ; Kluwer Academic Publishers 2015 Journal of neuro-oncology Vol.121 No.1

<P>Romo1 is a mitochondrial protein whose elevated expression is commonly observed in various types of human cancers. However, the expression status of Romo1 and its implication in the pathogenesis of human glioblastoma (GBM) remain largely undefined. To understand the role of Romo1 in the progression of GBM, we explored its expression in a series of GBM tissues and cell lines and determined its effect on ROS production, cell proliferation, and tumor growth. Romo1 was frequently overexpressed at the mRNA level in both primary tumors and cell lines and its elevation was more commonly observed in high grade tumors versus low grade tumors. Romo1 expression was associated with ROS production and its knockdown led to a marked reduction of in vitro cellular growth and anchorage-independent growth of GBM. Consistently, Romo1 depletion induced a G2/M arrest of the cell cycle that was accompanied with accumulation of phospho-cdc2. Furthermore, a mouse xenograft assay revealed that Romo1 depletion significantly decreased tumor formation and growth. Therefore, our data demonstrate that Romo1 upregulation is a common event in human GBMs and contributes to the malignant tumor progression, suggesting that Romo1 could be a new therapeutic target for human GBM.</P>

Managing Complicated Diabetic Foot Ulcers with Peripheral Arterial Disease

Yu Sung Hoon,Kim Dong Chul,Shin Chi Ho 대한창상학회 2020 Journal of Wound Management and Research Vol.16 No.3

Background: Diabetic foot ulcers are an issue of clinical interest as diabetes has become the leading cause of occlusive vasculopathy. The purpose of this study is to evaluate the clinical outcomes and the effect of revascularization in diabetic foot ulcers with peripheral arterial occlusive disease (PAOD).Methods: A total of 46 patients who had surgical treatment for diabetic foot ulcers were evaluated. We conducted an intergroup comparison of their baseline demographic and clinical characteristics, and also compared surgical outcomes between cases with and without PAOD, and also between cases on which revascularization had and had not been performed.Results: Major amputation was performed on 10 patients (21.7%). Minor amputation and reconstructive surgery were performed on 24 patients (52.2%) and 12 patients (26.1%), respectively. In the 19 patients (41.3%) with severe obstruction, eight patients (42.1%) underwent major amputation. In the patients without severe obstruction, two patients (7.4%) underwent major amputation. The high proportion of major amputation in patients with severe obstruction was statistically significant (P<0.01). Among the 19 patients with severe obstruction, nine (47.4%) underwent revascularization and 10 patients (52.6%) did not. In the patients who had revascularization, one patient (11.1%) had major amputation. In the patients who did not have revascularization, seven (70.0%) underwent major amputation. The low proportion of major amputation in patients undergoing revascularization was statistically significant (P=0.02).Conclusion: Results of this study demonstrate that preoperative evaluation of peripheral arterial disease and revascularization are important for reducing the risk of major amputation.

Apoptosis induced by methanol extract of <i>Potentilla discolor</i> in human mucoepidermoid carcinoma cells through STAT3/PUMA signaling axis

Yu, Hyun-Ju,Ahn, Chi-Hyun,Yang, In-Hyoung,Won, Dong-Hoon,Jin, Bohwan,Cho, Nam-Pyo,Hong, Seong Doo,Shin, Ji-Ae,Cho, Sung-Dae D.A. Spandidos 2018 MOLECULAR MEDICINE REPORTS Vol.17 No.4

<P><I>Potentilla discolor</I> has been used in traditional Chinese medicine for the treatment of hyperglycemia. However, the potential role of <I>Potentilla discolor</I> against cancer and its mode of action remain to be fully elucidated. The present study explored the apoptotic effect of methanol extract of <I>Potentilla discolor</I> (MEPD) in human mucoepidermoid carcinoma (MEC) cell lines of salivary glands. MEPD markedly suppressed the growth and induced apoptotic cell death in MC3 and YD15 cells. MEPD treatment significantly upregulated the expression of PUMA and reduced STAT3 phosphorylation. Overexpression of STAT3 partially recovered the growth of MEC cells inhibited by MEPD. In addition, dephosphorylation of STAT3 by cryptotanshinone (a potent STAT3 inhibitor) was sufficient to inhibit the growth of MEC cells and induce apoptosis via affecting PUMA protein. These results suggest that MEPD has a potential anticancer property via the STAT3/PUMA signaling axis in human MEC cells of salivary gland.</P>

Alpha-naphthoflavone induces apoptosis through endoplasmic reticulum stress via c-Src-, ROS-, MAPKs-, and arylhydrocarbon receptor-dependent pathways in HT22 hippocampal neuronal cells

Yu, Ah-Ran,Jeong, Yeon Ju,Hwang, Chi Yeon,Yoon, Kyung-Sik,Choe, Wonchae,Ha, Joohun,Kim, Sung Soo,Pak, Youngmi Kim,Yeo, Eui-Ju,Kang, Insug Elsevier 2019 NeuroToxicology Vol.71 No.-

<P><B>Abstract</B></P> <P>α-Naphthoflavone (αNF) is a prototype flavone, also known as a modulator of aryl hydrocarbon receptor (AhR). In the present study, we investigated the molecular mechanisms of αNF-induced cytotoxic effects in HT22 mouse hippocampal neuronal cells. αNF induced apoptotic cell death via activation of caspase-12 and -3 and increased expression of endoplasmic reticulum (ER) stress-associated proteins, including C/EBP homologous protein (CHOP). Inhibition of ER stress by treatment with the ER stress inhibitor, salubrinal, or by CHOP siRNA transfection reduced αNF-induced cell death. αNF activated mitogen-activated protein kinases (MAPKs), such as p38, JNK, and ERK, and inhibition of MAPKs reduced αNF-induced CHOP expression and cell death. αNF also induced accumulation of reactive oxygen species (ROS) and an antioxidant, N-acetylcysteine, reduced αNF-induced MAPK phosphorylation, CHOP expression, and cell death. Furthermore, αNF activated c-Src kinase, and inhibition of c-Src by a kinase inhibitor, SU6656, or siRNA transfection reduced αNF-induced ROS accumulation, MAPK activation, CHOP expression, and cell death. Inhibition of AhR by an AhR antagonist, CH223191, and siRNA transfection of AhR and AhR nuclear translocator reduced αNF-induced AhR-responsive luciferase activity, CHOP expression, and cell death. Finally, we found that inhibition of c-Src and MAPKs reduced αNF-induced transcriptional activity of AhR. Taken together, these findings suggest that αNF induces apoptosis through ER stress via c-Src-, ROS-, MAPKs-, and AhR-dependent pathways in HT22 cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> α-Naphthoflavone (αNF), an arylhydrocarbon receptor modulator (AhR), induces apoptosis in HT22 hippocampal neuronal cells. </LI> <LI> ER stress plays a role in αNF-induced apoptosis. </LI> <LI> The c-Src-, ROS-, MAPK-, and AhR-dependent pathways are implicated in αNF-induced ER stress and apoptosis. </LI> <LI> The AhR nuclear translocator-dependent genomic pathway also mediates αNF-induced ER stress and apoptosis. </LI> </UL> </P>