Agar-Gel Immunodiffusion Test를 이용한 돼지흉막폐염(Swine Pleuropneumoniae)의 진단에 관한 연구

심항섭,장경수,조용성,전무형 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-

To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, that is the primary agent of swine pleuropneumoniae, the methods for micro-agglutination test(MAT) and agar-gel immunodiffusion test(AGID) were improved and standarized and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with MAT and AGID. The range of antibody titers on MAT was 80 to 1280, showing the cross-reaction in serotypes 3, 4, 5 and 6. In AGID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. The optimal concentration of antigen MAT were 1.25㎎/㎖ 2. In AGID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer(pH 7.2), respectively. 3. By the tests for 200 swine sera, it was found that the accumulative frequency of positive reaction for the 6 serotypes were 240 in MAT and 163 in AGID. 4. when compared the results of AGID with those of MAT for 200 swine sera, all sera with MAT titier under 20 were negative in AGID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in AGID, respectively. All sera with MAT titer over 160 showed positive in AGID. 5. In conclusion, the established MAT showed high sensitivity but low specificity, whereas AGID revealed low sensitivity but high specificity. The established AGID test was considered very efficient for detection of antibodies and serotyping of swine sera.

開業醫 硏修敎育에 關한 調査 硏究

沈雲澤,李棟培,曺永釵 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

For the purpose of analyzing the opinions of medical practitioners who already took lectures of medical practitioner training seminars, we conducted the surveys from April I through June 30, 1984. Total subjects were 472 nationwide medical practitioners and their opinion about present training seminar system and about improvement of that in the future were analyzed. Followings are the conclutions therefrom: 1. Of the lectures of medical practitioner training seminar, 47. 5% were sponsered by provincial medical society, 25. 5% were scientific lecture meetings of sectional society and 21.5% were professional chair of medical college. 61. 9% of subjects responsed "may be acceptable", 14.6% were "difficult to grasp the content of lecture" and "no interest", but 6. 9% responsed "interest" 2. About the effect of lectured training seminar, 40.2%, of subjects responsed "clinically" practicable", 34.8% were "modern knowledge but clinically cannot practicable" and 22:0%. were "too theoretical to clinical use" in that order. 3. Only 62. 7% of sudjects knew the scores acquired after training seminar. 59.7% of subjects didn't know the regulation on the shortage of aqcuired scores. 4. For the reason that obstacles about the lecture of training seminar, 47.5% were lack of time, 22. 1% were inappropriate lecture place and 16. 1% were not good details of seminar. 5. As for the opinion about the contributions of lectured seminar, the follow: Acquisition of knowledge about modern medicine and drug 44. 5%, reinforcement of knowledge about clinical medicine 20.1% helpful in the clinical use 12.9,% and promote mutual friendship 6.2%. 6. The places of training seminar wanted by subjects were as it is with 35.5%, lecturing tour to the Eup and Myon with 27.8%, university hospital and training hospital with 20.8%, establish the training course to the university hospital with 13. 3%. 7. Details and methods of training seminar were distribute the teaching material with 42.2%, for the practical technique with 30. 1%, theoretical lectures with 24.6%. And the fields of training seminar were without consideration of major field were 53.4%, consideration of major field only with 40.9%. 20. 4% wanted October for the time of training seminar, 16.3% on November, other months were revealed lower rate.

肺結核患者의 難治化에 미치는 要因에 關한 調査硏究

沈雲澤,李棟培,曺永釵 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

To find out the factors affecting to the intractability of pulmonary tuberculosis, we analyzed the 1,047 pulmonary tuberculosis patients, registered and treated at the Clinic of Chungnam Branch of Korean National Tuberculosis Association for entire 8 years from January 1st, 1978 to the December 31st, 1985. Following variables were find out to be the affecting factors to the intractability of pulomonary tuberculosis. 1. General characteristics : age of patient, residential area, education, occupation, marital status and smoking status; above 30 years of age, rural area, low educational background, physical workers and heavy smokers were more apt to be intractable pulmonary tuberculosis patient. 2. Laboratory findings : chest P-A, direct sputum smear and culture, and rate of antituberculous drug resistance; moderately advanced and far advanced, smear and culture highly positive and higher occurrence rate of antituberculotic resistance. 3. Medical facility they treated : primary and retreatment; the patient who treated at the drug store and health center were find out: more easily become intractable pulmonary tuberculosis patient.

척추후측만곡환자의 폐기능에 관한 연구 : Ⅰ. 指極間長을 사용한 正常身長 예측법 Estimation of Height from Arm span

심영수,조수헌 최신의학사 1984 最新醫學 Vol.27 No.5

택시 운전기사들의 유병상태에 관한 조사 연구

심운택,이종연,오장균,조영채 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

A questionnaire survey was conducted on 572 taxi drivers in Taejeon City to investigate the prevalence rate which was derived from the subjective symptoms of respondents according to the working condition and daily life effected on health. The disease were classified the cardiovascular disease, gastrointestinal disease, neurotic disease and musculoskeletal disease according to the subjetive symptoms. The results obtained were as follows : 1. Among the subjective symptoms. the complaint rate of latigability, eye strain, loss of appetite, irritability, dizziness on standing, stiffness on shoulder. and low back pain became decreased in that order. 2. The prevalence rates of cardiopulmonary and gastrointestinal disease were low in the old aged group, and the prevalence rates of all disease was proportional to the frequencies of accident experience. but no evident trend in job conditions. 3. The groups having regular meal time and rest after meal showed low prevalence rate in all disease. The sleeping time and smoking habit were related to the neurotic and cardiopulmonary disease. 4. The group of usual drug intake occupied 68% of all studied subjects, the age group of 40 or more and the group of having G-1 disease showed high rats of usual drug intake.

니세틸 정(아세틸-엘-카르니틴 500 mg)에 대한 뉴로세틸 정의 생물학적 동등성

조혜영,김은아,정현철,심영순,임동구,오인준,문재동,이용복 전남대학교 약품개발연구소 2001 약품개발연구지 Vol.10 No.-

Acetyl-L-carnitine (ALC), an endogenous component of the L-carnitine family, is naturally occurring molecule synthesized from L-carnitine (LC) by carnitine acetyl transferase. ALC has been shown to improve the cognitive performance of patients suffering from dementia of the Alzheimer's type and proposed for treating Alzheimer's disease in pharmacological doses. The purpose of the present study was to evaluate the bioequivalence of two ALC tablets, Nicetiler^TM (Dong-A pharmaceutical Co., Ltd.) and Neurocetil^TM (Kyung-Dong Pharmaceutical Co., Ltd.), according to the guidelines of Korea Food and Drug Administration. Twenty six normal male volunteers, 22.80±2.76 year in age and 63.07 7.98㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After one tablet containing 500㎎ of ALC was orally administered, blood was taken at predetermined time intervals and the concentrations of ALC in serum were determined using HPLC with fluorescence detector. Because of the presence of endogenous ALC, the calibration was performed using dialyzed serum. Pharmacokinetic parameters such as AUC_t, C_max and T_max were calculated and ANOVA was utilized for the statistical analysis of the parameters. The results showed that the differences in AUC_t, C_max and T_max between two tablets were 2.72%, -0.65% and -8.42%, respectively, when calculated against the Nicetile^TM tablet. The powers (1-β) for AUC_t and C_max were 94.87% and 87.17%, respectively. Minimum detectable differences (Δ) at α=0.05 and 1-β=0.8 were less than 20% (e.g., 15.58% and 19.16% AUC_t and C_max, respectively). The 90% confidence intervals were within ±20% (e.g., -11.84∼6.41 and -10.57∼11.88 for AUC_t and C_max, respectively). Two parameters met the criteria of KFDA for bioequivalence, indicating that Neurocetil^TM tablet is bioequivalent to Nicetile^TM tablet.

랫드의 생체내 카드뮴 축적에 대한 아연의 영향

심상일,조영채,이동배 忠南大學校環境問題硏究所 1993 環境硏究 Vol.11 No.-

This study was performed to find out the protective effects of zinc against accumulation of cadmium in rats. The exprimental rats were divided into two groups ; one group was administrated cadmium alone(cadmium alone group) and the other group was administrated simultaneously Cd and Zn(cadmium alone group ; Cd-I group ; 200ug/ml, Cd-II group ; 400ug/ml). Each group was administrated with different doses of cadmium (200ug/ml∼400ug/ml) and zink(100ug/ml∼1,000ug/ml) orally bia drinking water for two weeks. The gained Body weight and organ weight, cadmium levels of blood liver, kidney and spleen, hematogical values and enzyme activites were investigated for the experimental groups. The results were summarized as follows : 1. The body weights of exprimental rats were temporarily reduced, but gredually incresed after one week in each group. The weights of liver and kidney of the Cd-II(Cd 400ug/ml) group were significantly reduced compared with the control group(P<0.05). 2. The accumulation levels of Cd in the blood of rats were significantly reduced in the Cd-I group according to the Zn level was increased. But the Cd levels were significantly increased in the Cd-II group according to the Zn level was increased. 3. The accumulation levels of Cd in liver were reduced in the Cd and Zn mixed groups than the Cd alone group in the Cd-I group, but, in Cd-II group, the levels of Cd were increased in the Cd and Zn mixed groups than Cd alone group(p<0.01). 4. The accumulation levels of Cd in Kidney were significantly increased in the Cd and Zn mixed groups than Cd alone group in the Cd-I group(P<0.05), but, in the Cd-II group, the levels of Cd were reduced in the Cd and Zn mixed groups than the Cd alone group. 5. The accumulation levels of the Cd in spleen were significantly reduced in the Cd and Zn mixed groups than Cd alone group in the Cd-I group(P<0.05), but, there was not significantly differences in the Cd-II group. 6. The hematological values (RBC, WBC, Hb and Hct) were higher levels in the Cd alone group than the cd and Zn mixed groups in the Cd-I and Cd-II groups. 7. The activities of GOT, GPT, ALP, and LDH were significantly increased in the Cd alone group than the control groups, but, there was not signigicant in the Cd and Zn mixed group. According to the above results, this study suggests that Zn may accelarate the accumulation of Cd within various organs, but this metal had reducing effects on the toxicity of Cd to the hematological values and enzyme activities when the Zn was administrated simultaneously with Cd to the rats.

공단 근로자들의 혈증 중금속 농도에 관한 비교 연구

심운택,이동배,조영채,이영수,윤창규 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

For the purpose to estimate the exposure levels of heavy metals and to present a basis of occupational health managements to the industrial workers exposed to heavy metals, this study conducted to know the lead and chromium levels of whole blood of industrial workers. The study population were divided into three groups to the exposure levels ; directed exposure group, indirect exposure group and non-exposure group, and the sample number of workers who exposed to lead were 80, chromium were 77, and control group were 31. The contents of lead and chromium were analyzed by atomic absorption spectrophotometer. The results were summerized as follows : 1. Sex distribution of studied group were 77.1 % in male, and 22.9 % in female. Age distribution were 74.4% in 30-49 age group. The work in years were 43.1 % in 5-10 years, 29.8% in 1-4 years. 2. Mean blood level of lead were 33.53㎍/㎗ in direct exposure, 23.84㎍/㎗ in indirect exposure group and 11.77㎍/㎗ in control group. 3. Mean blood levels of chromium were 1.9㎍/㎗ in direct exposure, 1.51㎍/㎗ in indirect groups and 0.95㎍/㎗ in control group. 4. Mean lead levels of direct exposure group was higher in male than female as 36.44㎍/㎗, 27.35㎍/㎗ respectively, but there was no difference in indirect and control group by sex. Mean chromium levels of direct exposure group were higher levels 2.26㎍/㎗ in female than 1.84㎍/㎗ in male, but there were no difference in indirect and control group by sex. 5. The age group of 29 or younger among the direct exposed had high level of lead and chromium as 35.7㎍/㎗ 2.41㎍/㎗ respectively, but that age group had no evident difference in the level of lead and chromium among the indirect exposed and non-exposed.

일부 농촌지역 노인들의 인식기능에 관한 조사 연구

심운택,배일훈,오장균,조영채 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

For the purpose of promotion of mental health in the rural elderlies, we surveyed 506 elderlies by means of Mini-Mental State Examination(MMSE) for evaluation of cognitive functions. We assesed degree of cognitive function impairments and association between cognitive function and socio-environmental conditions. The results were as follows : 1. Proportion of severe and mild cognitive function impairments were increased as incresing age, and mean scores of MMSE became decreased significantly among them(P<0.001). 2. In female, rates of severe and mild cognitive function impairment were high and mean scores of MMSE were significantly lower than male(P<0.001). 3. In widow group, proportion of severe and mild cognitive function impairment were high and mean scores of MMSE were significantly low(P<0.001). 4. As decreasing familly numbers, proportion of severe and mild cognitive function impairment became high and mean scores of MMSE were significantly low(P<0.01). 5. In physical handicaped group, proportions of severe and mild cognitive function impairment were high and mean scores of MMSE were significantly low(P<0.001). 6. In low scores of physical ability group, proportions of severe and mild cognitive function impairment were high and mean scores of MMES were significantly low(P<0.001). 7. Most item-scores of MMSE were significantly correlated. 8. In stepwised multiple regression analysis, age, existence of physical disorder, sex and scores of physical ability were selected highly correlated variables and power of explanation was 0.1528.

푸마르산으로부터 숙신산 생물전환을 위한 Enterococcus faecalis RKY1 균체의 포괄담체에 의한 고정화

위영중,조영심,류화원 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

The Enterococcus faecalis RKY1 cells were immobilized within the polymer matrices such as agar, Ca-alginate, and k -carrageenan in order to investigate bioconversion characteristics of immobilized Enterococcus faecalis RKY1. Agar and k-carrageenan gels were prepared for cubic type, and Ca-alginate gels for bead type. When the seed volume of immobilized cells was adjusted to 10%(v/v), the optimum concentrations of agar, sodium alginate, and k -carrageenan for immo- bilization of Enterococcus faecalis RKY1 cells were 2%(w/v) for all. In this condition, the conversion ratios were 93.6, 96.4, 76.5%, respectively. Immobilized cells entrapped in agar, Ca-alginate, and k -carrageenan have all the optimum pH of 7.0. The optimum temperature of immobilized cells was 40℃ for agar gels, 35℃ for Ca-alginate gels, and 40℃ for k -carrageenan gels. Therefore, Ca-alginate gels were suitable for immobilization of Enterococcus faecalis RKY1 for succinate production.