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Agar-Gel Immunodiffusion Test를 이용한 돼지흉막폐염(Swine Pleuropneumoniae)의 진단에 관한 연구
심항섭,장경수,조용성,전무형 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-
To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, that is the primary agent of swine pleuropneumoniae, the methods for micro-agglutination test(MAT) and agar-gel immunodiffusion test(AGID) were improved and standarized and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with MAT and AGID. The range of antibody titers on MAT was 80 to 1280, showing the cross-reaction in serotypes 3, 4, 5 and 6. In AGID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. The optimal concentration of antigen MAT were 1.25㎎/㎖ 2. In AGID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer(pH 7.2), respectively. 3. By the tests for 200 swine sera, it was found that the accumulative frequency of positive reaction for the 6 serotypes were 240 in MAT and 163 in AGID. 4. when compared the results of AGID with those of MAT for 200 swine sera, all sera with MAT titier under 20 were negative in AGID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in AGID, respectively. All sera with MAT titer over 160 showed positive in AGID. 5. In conclusion, the established MAT showed high sensitivity but low specificity, whereas AGID revealed low sensitivity but high specificity. The established AGID test was considered very efficient for detection of antibodies and serotyping of swine sera.
심항섭,양수정,우종태,김혜성,이성식 한국동물위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.2
Bovine brucellosis has occurred for years in Gyeonggi province under the national test and slaughter scheme. The serum agglutination test (SAT) is a diagnostic tool to confirm the disease despite the argument on its specificity. We selected 8 farms where only one or two individuals were diagnosed as brucellosis through SAT at the primary regular herd check and isolated the causative organism and characterized the species by species-specific PCR. The pathogen isolation was successful in 6 farms out of 8 farms by microbiological culture, showing the successful rate of 75%. The isolation rate of the causative organism represents 70% from supra-mammary lymph node and 60% from uterine tissues. They were characterized as Brucella abortus biovar 1 after biotyping by PCR, showing the fragment of 498 bp. Five of 8 farms were diagnosed as brucellosis two to four times more over the intervals of two or three months. Here in this study we briefly showed the correlation of the sporadic outbreak of brucellosis tested by SAT and the isolation of the causative organism. Moreover one or two reactors against brucellosis among considerable size of herd may indicate that SAT failed to detect potentially infected individuals in the incubation stage or chronic phase of the disease.
경기도에서 발생하는 유우부루셀라병에 관한 연구;I. 감염우의 역학조사 및 분리균의 특성에 관하여
심항섭,고태오,유성종,우종태,박병옥,김성렬,박유순 한국동물위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.3
The survey was performed to provied details about the pattern of bovine brucellosis occurred in Kyunggi province area. The results obtained through the investigations were summarized as follows. Five hundred seventy - three cattle of bovine brucella reactor were occurred in 14 districts among 31 districts in Kyunggi province in 1989-1995. Among them, 370 cattle(64.5% ) were bred in Eastern area(Ichon, Yeju, Kwangju) and 153 cattle(26.7% ) in Southern area(Yongin, Ansung, Peungtack) And the number of farms occurred by bovine brucellosis was 110 ones. When we investigated the occurrance frequency for the 110 farms, the ratio of farms which was brocken out just one time was 67.3a and more than twice was 32.7%. In 573 cattle, 271 cattle were reoccurred in farms which had broken out the bovine brucellosis more than one time. And this survey said the interval of reoccurrance was like this ; within a month 50.2%, within two month 19.2%, within four month 7.4%, within six month 7.4%, within an year 15.1%, within 2 year 7.0%. Brucella abortus was isolated from 38 cattle of the 61 ones cattles, and in type all isolated belong to biotype 1.
Monocrotophos poisoning in wild mallards(Anas platyrhynchos)
심항섭,김혜성,우종태,김인섭,정해선,송은아,박준조 한국동물위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.4
The toxicity of organophosphate arises from disruption of the nervous system due to the inhibition of cholinesterase enzymes, leading to death. Six dead mallards were found at Ansung where is one of the most popular wintering sites for migratory birds in Korea, and requested for diagnosis to Gyeonggi Veterinary Service on January of 2007. Some examinations including polymerase chain reaction(PCR) could not find any evidence of specific disease condition. However, the contents of gastrointestinal tracts of the birds contained residues of monocrotophos ranged from 31.3ppm to 294.3ppm by gas chromato-graphy and mass spectrometry. It can be supposed that monocroptophos was responsible for the death of mallards by this results,