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Yoon, Jee-Hyun,Park, Ha-Yeong,Kim, Tae-Ju,Lee, Seung-Ho,Kim, Jung-Hoon,Lee, Guem-San,Kim, Hong-Jun,Ju, Young-Sung The Society of Korean Medicine 2014 대한한의학회지 Vol.35 No.4
Objectives: This study aimed for establishing the discriminative criteria for Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, which can be confused as 'Mokhyang', because of similar appearances and synonyms. Methods: Morphological characteristics of dried herbal medicines were compared by macroscopic observation. To examine microscopic features of 'Mokhyang', paraffin embedding and the staining by using Ju's method were conducted. Physicochemical experiments were performed using HPLC analysis and antioxidant assay. Results: The types of stem, phyllotaxy and leaf shape were chosen as macroscopic criteria and the size, oil spots, color and root top as well as phloem and central cylinder were compared using microscopic features. The HPLC results showed different amounts of costunolide and dehydrocostuslactone among Aucklandiae Radix, Inulae Radix and Vladimiriae Radix. However, Aristolochiae Radix only contained aristolochic acid. The antioxidant assays also showed that Vladimiriae Radix exhibited strongest antioxidant activity followed by Aucklandiae Radix, Inulae Radix and Aristolochiae Radix. Conclusions: These results demonstrate that macroscopic, microscopic and physicochemical methods would be useful tools for the discrimination of 4 kinds of 'Mokhyang'.
주인명,김수자,이강우,이명희,박금민,오현애,윤대두,김선자 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.4
저자들은 1990년 10월 28일 17세 초산부로부터 임신 44^+1주에 제왕절개술로 분만된 신생아에서 부검상 전신부종과 흉강, 심낭 및 복강내 삼출액 축적을 보였던 비면역성 태아수종 1에를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Nonimmune hydrops fetalis is defined as a pathologic and generalized abnormal accumulation of serous fluid in the entire body tissue and cavities unassociated with erythroblastosis. With the widespread introduction of immunoprophylaxis, there is a decline in rhesus isoimmunization and nonimmune hydrops fetalis (NIHF) becomes increasingly important. NIHF now accounts for 80% of all hydropic fetuses and its contribution to the perinatal mortality increased up to 3%. Now we report a case of NIFH with generalized edema, pericardial and pleural fluid accumulation and ascites found at autopsy. No other deformity was found.
대학생의 건강증진 프로그램 참여의사에 영향을 미치는 주요 요인에 관한 연구
김영복,하은희,김주영,윤영옥 韓國保健敎育.健康增進學會 2001 보건교육건강증진학회지 Vol.18 No.1
This study purposed to examine the participative intention for health promotion program in a university and to find out the factors to associate with the participative intention. The data were based on the self-reported questionnaires from 746 women who study in E university, and this survey performed May, 1998. This study performed to analyze the participative intention for health promotion programs and the factors associate with health promotion program using x2-test and trend test by the PC-SAS 6.12. The major findings were as fellows: 1. The tendency of participative intention for health promotion programs showed that Influenza preventive program was the highest among the health promotion programs, and the next were Weight control program, Rubella preventive program, Fitness program. On the other hand, Smoking preventive program and Non-drinking program were lower than the other program. 2. The four significant factors on participative intention for health promotion programs were grade, concern for health, and behavior change experience through the health education. On the other hand, the cognitive level for health, experience for health education were not the significant factors associate with the participative intention for health promotion programs. 3. The relationship between factors and each health promotion program showedthat Rubella preventive program, Influenza preventive program, Weight control program, Smoking cessation program and Non-drinking program were associated with the grade or the health concern. And Chronic diseases preventive program was associated with the grade and the concern for health. Fitness program and Sex education program were associated with the concern for health and the behavior change experience through health education.
영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화
정주령,김하나,박열,김민정,오영주,신수정,최윤정,김경호 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.1
Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.
Yoon, Ju-Yeon,Hong, Jin-Sung,Kim, Min-Jea,Ha, Ju-Hee,Choi, Gug-Seon,Choi, Jang-Kyung,Ryu, Ki-Hyun The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.4
A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased hot pepper plant and its biological and molecular properties were compared to that of PMMoV-J and PMMo V -So The genomic RNA of PMMoV-Kr consists of 6,356 nucleotides. The nucleotide and amino acid sequences identities of four viral proteins and two noncoding regions among PMMoV-Kr, PMMoV-S and PMMoV-J were $96.9\%\;to\;100.0\%\;and\;97.5\%\;to\;98.6\%$, respectively. Full-length cDNA amplicon of PMMoV-Kr was directly amplified by RT-PCR with a set of 5'-end primer anchoring T7 RNA promoter sequence and 3'-end virus-specific primer. Capped transcript RNAs from the full-length cDNA clone were highly infectious and caused characteristic symptoms of wild type PMMoV when mechanically inoculated to systemic host plants such as Nicotiana benthamiana and pepper plants.
FISH Karyotype Analysis of Fagopyrum tartaricum Gaerth (Tartary buckwheat) using Repetitive DNAs
Yoon-Ha Ju,Franklin Hinosa Mancia,Seyong Yoon,Heekyung Park,Soeun Yoon,Jiin Park,Sunmi Joung,Su Jeong Kim,Yul Ho Kim,Hwang Bae Sohn,Ki-Byung Lim,Hyun Hee Kim,Yoon-Jung Hwang 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.10