제주 성상 S/S MTr #1 행원 풍력단지 전압해석

김상준(Sang-Jun Kim),조민호(Min-Ho Cho),윤기갑(Gi-Gab Yoon),장상옥(Sang-Ok Jang),안정식(Jeong-Shik Ahn) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.11

S-344 : The Role of Skeletal Muscle Mass in Development of Metabolic Syndrome

( Byung Sam Park ),( Jun Sung Moon ),( Ji Sung Yoon ),( Kyu Chang Won ),( Hyoung Woo Lee ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Introduction: It has been well known that abdominal adiposity is related with insulin resistance and greater risk of metabolic disorder. Skeletal muscle plays central role in insulin mediated glucose disposal of whole body, but we only know a little about the association between skeletal muscle mass and MetS (MetS). The aim of this study is to clarify the clinical role of skeletal muscle mass in developing MetS through using skeletal muscle parameters by body impedance analysis (BIA). Methods: 1,042 healthy adults aged from 20 to 75 years who visited Yeungnam university health promotion center from Jun. 2008 to Jun. 2010 were enrolled. 204 subjects who had prior MetS or chronic disease that can affect to skeletal muscle mass were excluded. After 24 months from baseline, the metabolic parameters were assessed and the development of MetS was diagnosed using modified NCEP-ATP III criteria. From the BIA (Inbody 720), we obtained skeletal muscle mass (SMM, Kg), body fat mass (BFM, Kg), and visceral fat area (VFA, cm2). Then, we had defined as follows; percent of skeletal muscle mass (SMM%, %): SMM (kg)/weight (Kg), skeletal muscle index (SMI, Kg/m2): SMM (Kg)/height (m)2, skeletal muscle to body fat ratio (MFR): SMM (kg)/BFM (Kg) and skeletal muscle to visceral fat ratio (SVR, Kg/cm2): SMM (Kg)/VFA (cm2) Results: Mean follow up periods were 28.7±5.4 months. Among total 838 subjects (46.9±9.9 years, M:F=477:361), 88 (10.5%) were newly diagnosed MetS. 5th quintile of the SMM%, MFR and SVR was associated with decreased risk of development of MetS after adjusting for confounding factors. Conclusion: Decreased skeletal muscle mass may play critical role in development of the MetS and not absolute amount of skeletal muscle mass but relative ratio to body composition may be more important.

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

( Ji Yong Kim ),( Jai Sung Lee ),( Yong Seok Han ),( Jun Hee Lee ),( Inhyu Bae ),( Yeo Min Yoon ),( Sang Mo Kwon ),( Sang Hun Lee ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although H2O2 (200 mM) increased intracellular ROS levels in human MSCs, lycopene (10 μmM) pretreatment suppressed H2O2-induced ROS generation and increased survival. H2O2-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by H2O2 treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

Kim, Ji Yong,Lee, Jai-Sung,Han, Yong-Seok,Lee, Jun Hee,Bae, Inhyu,Yoon, Yeo Min,Kwon, Sang Mo,Lee, Sang Hun The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although $H_2O_2$ ($200{\mu}M$) increased intracellular ROS levels in human MSCs, lycopene ($10{\mu}M$) pretreatment suppressed $H_2O_2$-induced ROS generation and increased survival. $H_2O_2$-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by $H_2O_2$ treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

동종 반월상 연골 이식술의 임상적 결과 - 내측과 외측 및 동반 손상의 유무에 따른 비교 -

조승목,윤경호,이정환,배대경,송상준,강창민,Cho, Seung-Mok,Yoon, Kyoung-Ho,Lee, Jung-Hwan,Bae, Dae-Kyung,Song, Sang-Jun,Kang, Chang-Min 대한관절경학회 2009 대한관절경학회지 Vol.13 No.1

목적: 동종 반월상 연골 이식술의 결과를 내측과 외측 및 동반손상 유무에 따라 비교하고자 하였다. 대상 및 방법: 1999년 12월부터 2007년 6월까지 동종 반월상 연골 이식술을 시행하고 1년이상 추시가 가능했던 52례를 대상으로 하였다. 내측 이식군은 19례, 외측 이식군은 33례였고, 단독 손상군은 18례 동반 손상군은 34례였다. 평균나이는 34.2세(18세~51세) 평균 추시기간은 42.2개월(12개월~90개월)이었다. 임상적 결과의 판정을 위하여 슬관절 운동범위, VAS 점수, IKDC 주관적 평가 점수, Lysholm 점수, Tegner 점수, 환자 만족도 및 이차 관절경 또는 추시 MRI를 시행하였다. 결과: 수술 후 평균 관절운동 범위는 130.3도 였다. VAS 점수는 수술 전 5.96점에서 3.05점으로, IKDC 주관적 평가 점수 46.5점에서 64.52점으로(p<0.001), Lysholm 점수는 61.94점에서 79.58점으로(p=0.0019) Tegner점수는 수술 전 2.97에서 3.62점으로 향상되었으며 수술의 만족도는 '매우만족', '대체로 만족'이라고 대답한경우가 61.5% 였다. 수술 후임상적 점수의 향상이 있었지만 내측과 외측 이식술 군간 그리고 단독 손상군과 동반 손상군 간의 통계적 유의한 차이는 없었다. 이차 관절경을 시행한 18례중 10례에서 이식된 동종 반월상 연골의 변연부의 치유 소견이 관찰되었고 6례에서 부분파열이 2례에서는 복합 파열이 관찰되었다. 추시 MRI 촬영이 가능하였던 16례에서 아탈구는 12례에서 관찰되었다. 결론: 반월상 연골 이식술 후 내측과 외측 이식군 간의 임상적 결과는 차이가 없었으며 동반된 연골 손상이나 불안정성에 대한 치료를 병행한 경우 동반손상의 유무는 결과에 영향을 미치지 않았다. Purpose: To compare the clinical outcomes after meniscus allograft transplantation between lateral and medial or isolated and combined procedure groups. Materials and Methods: Of the patients who had undergone arthroscopic meniscal allograft transplantation between Dec. 1997 and Jun. 2007, 52 patients were available for retrospective evaluation. Patients were grouped into lateral(33 cases) and medial(19 cases) transplant groups as well as those with isolated(18 cases) and combined(34 cases) procedure. The average age was 34.2 years and the mean follow-up period was 42.2 months. Postoperative range of motion (ROM), visual analog scale (VAS), International Knee Documentation Committee (IKDC) subjective score, Lysholm score, Tegner score, patient's subjective satisfaction, $2^{nd}$ look arthroscopy and MRI were evaluated retrospectively. Results: Mean postoperative ROM was $130.3^{\circ}$. The VAS showed an improvement from 5.96 to 3.05 at the last follow up. IKDC subjective score and Lysholm score also showed an improvement from 46.5 to 64.5 and from 61.9s to 79.58 respectively. Tegner score was improved from 2.9 to 3.6. Overall, 61.5% of patients reported they were completely or mostly satisfied with procedure. There were no significant differences noted between lateral and medial groups as well as isolated and combined groups. In 2nd look arthroscopy, 10 of 18 cases showed good pheripheal healing and there were 6 cases of partial and 2 of complex tear. We observed graft subluxation or extrusion in 12 of 16 cases who were evaluated with follow-up MRI. Conclusion: Meniscus allograft transplantation alone or in combination with other procedure showed an improvement in knee pain and clinical score. But there were no significant difference between lateral and medial groups or isolated and combined procedure groups.

Pathoanatomical Factors Responsible for Femoral Shortening in Legg-Calve-Perthes Disease

Sung-Man Rowe(노성만),Eun-Sun Moon(문은선),Myung-Sun Kim(김명선),Jun-Yub Lee(이준엽),Chang-Ich Hur(허창익),Tae-Yoon Ha(하태윤) 대한정형외과학회 2005 대한정형외과학회지 Vol.40 No.5

목적: Legg-Calve-Perthes 병(LCPD) 환자에서 대퇴골의 단축에 관여하는 병리해부학적 인자들의 상대적인 기여도를 결정하여 그 병리해부와 지단축의 진행과정을 이해하고, 이를 통해 지단축을 감소시킬 수 있는 방법을 찾아보고자 하였다. 대상 및 방법: 106명의 LCPD 환자들을 대상으로 원격 X-선 촬영법을 통해 대퇴골의 골두 골단, 경부 및 간부의 단축정도를 측정하였으며, 질병 시기별 분포는 활동기가 35명, 치유기가 24명 그리고 골성숙기가 47명이었다. 결과: LCPD 환아에 있어서 골성장 완료 후 대퇴골 단축은 골두 골단에서 20% (골단 편평화), 경부에서 53% (성장판의 성장 지연), 그리고 간부에서 27% (저사용에 의한 골위축)로 기여하고 있으며, 이러한 부위별 기여의 정도는 질병의 시기 및 단축의 정도에 따라 차이가 있음을 확인할 수 있었다. 또한 대퇴골 간부의 단축은 골성숙기 총 47명의 환아에 있어서는 평균 3.9 ㎜였으나, 47명 중 20 ㎜ 이상의 고도의 단축을 보인 15명의 환아에 있어서는 평균 5.8 ㎜의 단축을 보였다. 결론: 단축된 하지의 저사용 위축에 의한 대퇴골 간부의 단축은 환자의 운동부족이 원인의 하나로 생각되므로 적절한 운동처방으로 이를 예방할 수 있을 것으로 생각된다. Purpose: The purpose of this study was to determine the relative contributions made by pathoanatomical factors responsible for femoral shortening in Legg-Calve-Perthes disease (LCPD), and to devise a method of reducing the amount of residual shortening based on a better understanding of its pathoanatomy and developmental pattern in LCPD. Materials and Methods: We measured shortening of three anatomical components, namely, the femoral epiphysis, neck, and diaphysis on the teleoroentgenograms of 106 LCPD patients, comprised of 35 children with active disease, 24 in the healing stage, and 47 at skeletal maturity. Results: The proportional contributions made by these 3 anatomical components to residual shortening at skeletal maturity were; 20% by the epiphysis (epiphyseal flattening), 53% by the neck (physeal growth retardation), and 27% by the diaphysis (underuse atrophy). These contributions differed according to disease stage and shortening severity. Mean diaphyseal shortening was 3.9 ㎜ at skeletal maturity, but this increased to 5.8 ㎜ when only patients with severe shortening (20 ㎜ or more) were included. Conclusion: Our findings suggest that diaphyseal shortening is likely to be minimized by the implementation of limb exercise programs.

uPAR expression under hypoxic conditions depends on iNOS modulated ERK phosphorylation in the MDA-MB-231 breast carcinoma cell line

Yoon, So Young,Lee, Yoo Jung,Seo, Jae Hong,Sung, Hwa Jung,Park, Kyong Hwa,Choi, In Keun,Kim, Seok Jin,Oh, Sang Cheul,Choi, Chul Won,Kim, Byung Soo,Shin, Sang Won,Kim, Yeul Hong,Kim, Jun Suk Springer Science and Business Media LLC 2006 Cell research Vol.16 No.1

<P>Urokinase plasminogen activator receptor (uPAR) plays a major role in cancer invasion and metastasis and uPAR expression is correlated with a poor prognosis in various cancer types. Moreover, the expression of uPAR is increased under hypoxic conditions. Nitric oxide (NO) and its metabolites produced by inducible nitric oxide synthase (iNOS) are important products of hypoxic stress, and NO may activate or modulate extracellular signal regulated kinase (ERK). Here, we evaluated uPA, uPAR, and activated ERK levels under hypoxic conditions, and the modulatory effects of iNOS and NO in the MDA-MB-231 human breast cancer cell line. Cells were incubated in a hypoxic or normoxic incubator and treated with PD98059 (a MEK 1/2 inhibitor, which abrogates ERK phosphorylation) and aminoguanidine (a selective iNOS inhibitor). uPAR expression, ERK phosphorylation, and uPA activity were found to be increased under hypoxic conditions. Moreover, when cells were treated with PD98059 under hypoxic conditions, uPAR was downregulated, whereas aminoguanidine markedly increased ERK phosphorylation in a dose dependent manner. Furthermore, aminoguanidine increased uPAR expression and prevented the inhibition of uPAR expression by PD98059. These results demonstrated that uPAR is induced by hypoxia and that increased uPAR expression is mediated by ERK phosphorylation, which in turn is modulated by iNOS/NO in MDA-MB-231 cells. We conclude that iNOS/NO downregulates the expression of uPAR under hypoxic conditions via ERK pathway modulation.</P>

Assessment of Change of Intrahepatic Fat Amount Using Controlled Attenuation Parameter in Clinical Trial

( Sang Bong Ahn ),( Dae Won Jun ),( Jae Yoon Jeong ),( Joo Hyun Sohn ),( Chang Hong Lee ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Multi-echo modified Dixon (mDixon) sequence (MR-PDFF) is asafe and non-invasive alternative for the quantification of hepaticfat content. And it has accepted reasonable method to assess thechange of hepatic fat amount in phase II study. Recently controlledattenuation parameter (CAP) has been showed good correlation withintrahepatic fat amount compare to liver biopsy as well as MRS data in large cross sectional cohort. However there is little known whetherchange of CAP scores can be used in clinical trial. We investigatedthe correlation with CAP and MRS by serial examination in clinicaltrial setting.Methods: Sixty-five NAFLD patients were evaluated with MRS andtransient elastography including CAP in clinical study. Both MRS andCAP were evaluated after three month probiotic clinical trial in patientswith NAFLD.Results: Baseline CAP and MR-PDFF showed good correlation assessinghepatic steatosis (r=0.60, p<0.001). Also, changes of CAP value wasalso correlated with changes of intra-hepatic fat % using MR-PDFF(r=0.35, p=0.008) in clinical trial setting. Concordance rate of improvementor aggravation was comparable in both two methods. However,the less change amount was small in CAP value, the less concordancerate showed more weak with MR-PDFF. When the change of CAPvalue after treatment was less than 20, concordance rate withMR-PDFF was decreased to 15/25 (60%).Conclusions: CAP and MRS have a comparable diagnostic value forthe hepatic steatosis quantification as well as assessing changes ofhepatic fat amount in clinical trial. However, a careful interpretationof the steatosis change using CAP score should be given when theabsolute change value was less than 20 in clinical trial setting.

Efficacy and Safety of Entecavir and Tenofovir in Chronic Hepatitis B Patients with Chronic Kidney Disease: Multicenter, Retrospective Cohort Study

( Sung Eun Kim ),( Jae Yoon Jeong ),( Dae Won Jun ),( Eileen L. Yoon ),( Jae-jun Shim ),( Sang Bong Ahn ),( Yong Kyun Cho ),( Soung Won Jeong ),( Hyoung Su Kim ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: We investigate the efficacy and safety of entecavir (ETV) versus tenofovir (TDF) in treatment-naïve chronic hepatitis B (CHB) patients with chronic kidney disease (CKD) in real life setting. Methods: A total of 1,349 naïve CHB patients treated with ETV or TDF at least 1 year in 9 academic hospitals from Jan. 2012 to Dec. 2015 were enrolled. Among them, 44 patients had CKD at enrollment. CKD was defined as glomerular filtration rate <60ml/min/1.73m2. Renal functional decline was defined when serum creatinine level increased by more than 50% from baseline or over than 0.3 mg/dL at 1 year Results: A total of 44 patients (ETV=30 and TDF 14) were followed up for a median 28 months. There were no significant differences in cumulative biochemical response rate and culmulative HBeAg seroconversion rate between the ETV group and the TDF group at weeks 12, 24, and 48 of treatment. However, there were significant differences in culmulative virologic response rate between the ETV group and the TDF group at weeks 12, 24, and 48 of treatment (14%/31%/55% vs.14%/64%/86%, P =0.022). Renal functional decline occurred in 13 (29.5%) patients. Renal functional decline showed no significant difference between the ETV group and TDF group (33% vs. 21%, P=0.420). In multivariate analysis, serum albumin was an independent factor associated with renal functional decline (odd ratio, 0.28; confidence interval, 0.08- 0.95; P=0.041). However, age, sex, BMI, DM, hypertention, baseline HBV-DNA level, HBeAg positivity, cirrhosis, baseline renal function, bilirubin level, platelet count, hemoglobin level, ascites and treatment regimen were not associated with renal functional decline in CHB patient with CKD. Conclusions: TDF had better clinical efficacy than ETV in CHB patient with CKD. This study suggests that close monitoring of renal function are required for CHB patients with CKD and low albuminemia who are receiving antiviral agents.

Economic Analysis for Microalgal Removal of Urban-area Focused Carbon Dioxide from Industrial Flue Gas

Sung Jin SHIM,Yoon Young CHOI,Won Seok CHANG,Sang Jun SIM 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.4