Developmental Competence of Bovine Embryos Cultured In Vitro According to Culture Methods

Min CS,Son GD,Chung WJ,Song SH,Rho CW,Kang YS,Kong IK,Park CS 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.2

Developmental Competence of Bovine Embryos Cultured In Vitro According to Culture Methods

Min CS,Son GD,Chung WJ,Song SH,Rho CW,Kang YS,Kong IK,Park CS 한국동물번식학회 2007 Reproductive & Developmental Biology(Supplement) Vol.31 No.2s

Tau dephosphorylation is required for regulating Pin1 phosphorylation & Production of Alzheimer's diseased transgenic mice carrying NSE controlled human Tau

SH. Min,JS. Cho,JH. Oh,DY. Hwang,SH. Lee,SB. Shim,SW. Jee,HJ. Lim,SJ. Seo,YS. Song,HK. Park,YY. Sheen,YK. Kim(김용규) 한국실험동물학회 2004 한국실험동물학회 학술발표대회 논문집 Vol.2004 No.-

인화성 물질의 SPME 분석시 조건 비교

민지숙(Min JS),박우용(Park WY),진광호(Jin KH),박윤식(Park YS),박성우(Park SW) 한국법과학회 2002 한국법과학회 학술대회 Vol.2002 No.1

양수내 Creatinine치에 관한 연구

김영석(YS Kim),민병철(BC Min),이명아(MA Lee),박창서(CS Park),박찬용(CY Park),조성진(SJ Cho) 대한산부인과학회 1988 Obstetrics & Gynecology Science Vol.31 No.10

정확한 태아 성숙도 판정을 위해서 임신 제 35주에서 제 41주까지의 정상임부 35례, 자간전 증을 합병한 임부 28예에서 양수와 모체혈청의 creatinine concentration측정 및 shake test를 동시에 실시하였다. 그리고 전례에서 호흡장해증후군의 발생을 관찰하여 위의 검사결과에 입각한 태아 성숙도 판정을 시도하여 다음과 같은 결론은 얻었다. 1. 양수내 creatinine concentration 및 AF/MS비는 정상임신군 및 자간전증군에서 임신주수에 따라 점차로 증가하였다. 2. 자간전증군의 양수와 모체혈청 creatinine concentration 및 평균 AF/MS비는 정상임신군의 수치보다 유의하게 높았다. 3. 태아 성숙기준을 임신 제 37주이상으로 정하였고 양수 creatinine concentration 2.0mg%이 상, AF/MS비 2.5이상 그리고 positive shake test를 성숙기준으로 선택할 때 ,그 정확도는 정 상임신군에서는 85.7%, 82.9% 88.9%이었고, 자간전증군에서는 89.3% 95.7% 92.9% 이었다. 그리고태아체중 2500g을 성숙기준으로 하고 똑같은 방법으로 예측한 태아 성숙판정의 정확 도는 정상임신군에서는 88.6% 91.4% 85.7% 이엇고, 자간전증군에서는 75.0% 78.6% 75.0%ㅇ 었다. 4. 총 7예의 신생아에서 신생아 호흡장해증후군이 발생했는데 4례는 정상임신군에서 3례는 자간전증군에서였다. Accurate estimation of fetal maturity is neccessary where termination of pregnancy before term is indicated. To predict the fetal maturity creatine concentration of amniotic fluid and maternal serum were measured and shake test was performed simultaneously in the 35 normal pregnancy women and 28 preeclamptic women between 35 to 41 gestational weeks. The results were as follows; 1. The creatinine concentration of amniotic fluid and aminiotic fluid/maternal serum concentration of creatinine (AF/MS) ratio increased gradually related to gestational weeks in normal and preeclamptic pregnancy. 2. The mean amniotic fluid and maternal serum creatinine concentration and the mean AF/MS ratio in preeclamptic pregnancy were significantly higher than those inf the the normal pregnancy. 3. Assuming a creatinine concentration 2.0mg% or greater in the amniotic fluid, AF/MS ratio of 2.5 or greater and positive shake test to corespond with 37 weeks of gestational age, the accuracy in predicting fetal maturity was 85.7%, 82.9% and 88.9% in normal pregnancy and 89.3% , 95.7% and 92.9% in preeclamptic pregnancy, respectively. Assuming same indication to correspond with 2500 mg of fetal weight , the accuracy in predicting fetal maturity was 88.6%, 91.4% and 85.7% in normal pregnancy and 75%, 78.6% and 75% in preeclamptic pregnancy, respectively. 4. Total 7 neonates developed respiratory distress syndrome, four in normal group and remainder in preeclamptic pregnancy group.

자궁경부암 환자의 국소 침윤 판정에 있어서 MRI의 유용성에 관한 연구

강재성,민유선 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.3

1989년 3월부터 1996년 12월까지 만 7년 10개월 동안 고려대학교 병원 산부인과에서 자궁경부암으로 병리조직학적 진단을 받고 MRI를 이용한 임상병기 결정 후 수술적 치료의 적응증이 되는 환자를 자궁경부암 근치수술 및 골반 임파절 적출술을 시행받은 87명을 연구 대상으로 하여 병기설정시 MRI의 유용성을 알아보고자 수술적 치료 후 얻은 수술적 병기의 소견과 비교 연구하여 다음과 같은 결과를 얻었다. 1. 환자의 평균연령은 50.8±11.2세 였다. 2. 연구 대상 환자의 병리조직학적 분포는 편평상피 세포암이 94.3%, 선암이 5.7%이었다. 3. 임상병기와 수술적 병기를 비교하여 보면 전체적으로 49.9%의 정확도를 보였다. 임상 병기 Ⅰa1, Ⅰa2, Ⅰb, Ⅱa에서 수술적 병기와 일치하는 정확도는 각각 40.0, 47.1, 59.4, 64.3%로 병기가 증가함에 따라 약간씩 증가하나 통계적 의의는 없었다. 4. MRI를 이용한 병기와 수술적 병기를 비교하면 전체적으로 57.5%의 정확도를 보여 일치율이 낮은 것으로 나타났다. MRI 병기 Ⅰa, Ⅰb, Ⅱa, Ⅱb에서 수술적 병기와 일치하는 정확도는 각각 100, 45.2, 66.7, 16.7%이었고 Ia, Ib간에는 통계적으로 유의한 차이가 있었다. 5. MRI상 자궁경부 침윤 깊이의 판정은 MRI 상 침윤이 없어 미세 침윤암으로 판정된 Ⅰa 환자 12명 모두 경부 두께의 1/2 미만의 침윤이 수술적으로 규명되었고 MRI상 종양이 있는 경우에서도 41명[54.7%]이 1/2 미만의 침윤이 있어 MRI 소견만으로 자궁경부 침윤 깊이를 판정하기는 부적합하였다. 6. MRI상으로 질 침윤이 없을 것으로 예측한 환 자 54명 중 4명[7.4%]에서 병리조직학적으로 침습이 있음이 확인되었고 질 침습이 있을 것으로 예측한 33명 중 3명[9.1%]에서 병리조직학적으로 침습이 없는 것으로 나타났다. 7. MRI상으로 자궁방 결합조직으로의 침습이 없 을 것으로 판정된 81명 중 6명[7.4%]이 실제 침습이 있었고 침습이 있다고 판정된 6명 중 4명[67.7%]이 실제 침습이 없었다. 8. MRI상 골반 임파절 전이가 없을 것으로 판정된 86명 중 5명[7.4%]이 수술 후 전이가 확인되었다. 9. 자궁경부암에서 주위조직으로의 침습 여부를 MRI로 판정할 때 정확도, 민감도, 특이도는 질 침습에서는 각각 92, 88, 94%, 임파절 전이는 각각 94, 100, 94%, 자궁방 결합조직은 정확도 89%, 특이도 95%였다. 이상의 결과 MRI는 자궁경부암의 병기판정에 있 어서 특이도가 높은 검사법으로 사료되며 초기 경부암 수술적 치료환자의 선별에 유효하나 이를 근거로 한 임상병기의 설정은 실제 병기와 차이를 나타내는 경우가 많으며 향후 이러한 격차를 줄이기 위한 좀 더 정확한 진단법의 개발이 요구된다. Accurate staging prior to surgery is essential in the management of cervical carcinoma. To evaluate the usefulness of magnetic resonance imaging [MRI] in the clinical staging of cervical carcinoma, from March 1989 to December 1996, at Korea University Hospital, the accuracy of clinical staging and MRI staging were determined retrospectively in 87 consecutive patients in whom the extent of disease was surgically confirmed. The results were as follows ; 1. Clinical staging had an overall accuracy of 49.9% in the assessment of histopathologic extent of the cervical carcinoma. 2. Staging of cervical carcinoma solely based upon MRI revealed an accuracy rate of 57.5%. It had an accuracy of 100% for Ia and 45.2% for Ib, and this difference was statistically significant. 3. The accuracy rate of the radiologic assessment of invasion into vagina was 92%, 89% for parametrium, and 94% for lymph node, and its specificity in the evaluation of invasion into vagina was 94%, 95% for parametrium, and 94% for lymph node metastasis. We concluded that the MRI was proved to be a highly accurate and specific modality in the assessment of the locoregional extent of the cancer, but it had a limit in the assessing the depth of cervical stromal invasion. The clinical staging of cervical carcinoma using MRI would be more accurate in the way of refinement of the diagnostic tools and more judicious assessment of the extent of the diseaes.

부인과영역에 있어서 자궁내관경에 대한 임상적 관찰

민부기(BG Min),홍석호(SH Hong),유성현(SH Yoo),박영선(YS Park) 대한산부인과학회 1982 Obstetrics & Gynecology Science Vol.25 No.2

Hysteroscopy has added a new dimension to the management of patient with common clinical problems, increasing the accuracy of diagnosis and serving as an adjunct in treatment of intrauterine conditions. Here, we have studied differential aspects of approching in the hysteroscopic observation to the uterine cavity with 95 cases. The study was conducted at department of Obstetrics and Gynecology, Eul-Ji General Hospital in period of 20 months from Dec., 1978 to June., 1980. 1. The most popular age group for hysteroscopy was in age of 31-35 years. 2. As the anesthesia for hysteroscopy, general analgesia was used successfully in 72 cases, and paracervical block was used in remainder cases. 3. The time required for diagnostic hysteroscopy was taken 10-15 minutes in 36 cases(37.9%). 4. The most common indications for hysteroscopy were abnormal uterine bleeding in 43 cases, intrauterine adhesion in 23 cases and infertility in 18 cases. 5. On the abnormal uterine bleeding in women during the reproductive ages, cervical and endometrial polyp were most common in 13 cases of the 32 cases. 6. On the abnormal uterine bleeding in postmenopausal women, endometrial hyperplasia was most common in 5 cases of the 11 cases. 7. Intrauterine adhesions were classified into the 3 types as diffuse, 11 cases, central type 5 cases, and marginal type 7 cases. 8. On hysteroscopic finding of infertile women, unexplained infertility with normal uterine cavity was most common in 10 cases of the 18 cases. 9. Abnormal uterine cavity of infertile women was seen in 8 cases of the 18 cases. 10. Complicaions on hysteroscopy were occurred in 9 cases of all 95 cases, as uterine perforation, infection and profuse uterine bleeding.

Prostaglandin E2질정에 의한 유도분만의 효과

한선남(SN Han),김양숙(YS Kim),이진희(CH Lee),박숙현(SH Park),전정희(JH Jeun),민보인(BI Min) 대한산부인과학회 1985 Obstetrics & Gynecology Science Vol.28 No.1

In order to determine the clinical usefulness of Prostaglandin E2 vaginal pessary in the induction of labour a stable 3mgm Prostaglandin E2 pessary supplied by Korea Upjohn Ltd. was used in the induction of labour in 66 patients admitted to Il Sin Women`s Hospital, Busan, between November 1982 and February 1983. In 19 cases the maximum does of 6mgm was used, a second 3mgm pessarybeing inserted 8~15hrs after the first pessary. Results 1. PGE2 pessaryalone was successful in inducing labour in 32 patients, 48.5%, and was considered to be a definite aid in a further 19, 28.8%. 2. In addition to the 32 patients already delivered or in active labour the action of cervical ripending was seen in an additional 9 patients in that the Bishops score increased by 3 points within 15 hours after the insertion of one 3mgm pessary. 3. In cases successfully induced by PGE2 pessary the average interval between rupture of the membranes and delivery was very short. 2.7hrs, and the average duration of labour was 5.4hours. 4. The overall Cesarean section rate was 21.2% but none was done for failed induction. The main indication was C.P.D., 11 out of 14 patients. 5. The incidence of postpartum hemorrhage, that is a loss of 500cc or more, was slightly higher than the overall hospital incidence. 6. Side effects were minimal. One patient vomited but only 10mls and among 29 pre-eclamptic or eclamptic patients in only 10 was there a change in the blood pressure, an increase by 20mmHg in 7 and decrease by 20mmHg in 3, within 3 hours of insertion of the pessary. 7. There were no episodes of fetal distress that could be attributed to PGE2 administration. 8. The factors possibly affecting the success rate, Bishop`s score, parity, gestational age and indication for induction were analysed. The indication for induction and gestational age were significant factors but parity and Bishop`s score had no affect on the outcome. Among patients with hypertensive complications of pregnancy there was a high success rate but a very low success rate in patients with post mature pregnancies and in these cases apart from one patient there was no other obstetric indication for induction. The high success rate in preterm pregnancies was irrespective of parity of Bishops score but there was always a definite obstetric indication for induction in these cases and this is considered to be the more important factor. This study also shows that the PGE2 pessary is simple to use, acceptable to the patient, has almost no side effects and can be safely used in all cases in which vaginal delivery is possible with a high rate of success. It is planned to continue the use of PGE2 pessary when it is commercially available.

자궁외임신 환자에서 골반경수술과 개복술후의 임신율 비교

김유곤,박양서,장봉림,이우영,김두순,민술홍 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.2

본 한림대학교 강동성심병원 산부인과에서는 1991년 7월 1일부터 1992년 10월 31일까지 16개월동안 난관임신으로 수술후 추적이 가능했던 54예를 대상으로 골반경 수술과 개복술이 다음 임신율에 미치는 영향을 관찰하여 다음과 같은 연구결과를 얻었다. 1. 골반경 수술을 한 군과 개복술을 한 군의 연령, 임신력, 출산력, 과거불임 유무, 기왕개복술 유무, 과거 골반염증 유무 등에는 특별한 차이가 없었다(p$lt;0.05). 2. 골반경수술시 다음 임신은 11예(61.1%)이었으며 그중 자궁내임신은 9예(50.0%), 자궁외임신은 2예(11.1%)이었고, 개복수술시 다음 임신은 18예(50.0%)이었으며 그중 자궁내임신은 17예(47.2%), 자궁외임신은 1예(2.8%)이었다. 3. 수술방법에 관계없이 골반내 유착의 정도와 임신율은 역비례하였다(p$lt;0.05). 4. 복강내 출혈량은 수술방법에 따른 임신율에 별 영향을 미치지 못하였다(p$lt;0.05). 본 연구결과 좀더 많은 수술후 예후 관찰이 추가되어야 확실해지겠지만 난관임신에서 골반경 수술을 한 군은 개복수술을 한 군보다 이미 알려진 장점 외에 임신율에 있어서도 개복수술을 한 군과 비교하여 비슷한 자궁내임신율을 다음 임신에서 얻을 수 있을 것으로 사료된다. To compare pelviscopic surgery with laparotomy in the management of hemodynamically stable patients with tubal pregnancies, we reviewed 54 patients, visiting Hallym University Hospital from July 1991 to October 1992, future pregnancy rates in order to find factors that can affect pregnancy outcome. The results were as follows; 1. The demographic data of two groups including age, gravidity, parity and others were not different significantly. 2. The intrauterine pregnancy rates of pelviscopic surgery and laparotomy groups after operation were 50.0% (9/18), 47.2% (16/37) respectively.There were no statistical significance(p$lt;0.05). 3. Regardless of operation methods, pelvic adhesions were correlate to future pregnancy rates inverely(p$lt;0.05). 4. In two groups, amount of hemoperitoneum did not affect the pregnancy rates(p$lt;0.05). The results suggest that intrauterine pregnancy rates of pelviscopic surgery and laparotomy groups were not different significantly.

골반에 발생한 이소성폐흡충증 1 예 보고

강재성,최안나,민유선,조현이 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.6

최근에 본 교실에서 43세 환자가 하복부통을 주소로 내원하여 골반벽에 발생한 폐흡충증을 진단받고 절개배농 및 약물투여로 치료한 바 있어 간단한 문헌고찰과 함께 보고하는 바이다. It is well known fact that Paragonimus westermani causes ectopic parasitism of various sites in human hosts. Major clinical problems of the ectopic paragonimiasis are related to the involved organs. We experienced a case of pelvic paragonimiasis. Explorative laparotomy was performed and Paragonimus granulomas were noted. This case was postoperatively treated with praziquantel.